BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_N01
(545 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g18100.1 68418.m02125 superoxide dismutase [Cu-Zn] / copper/z... 104 4e-23
At1g08830.1 68414.m00983 superoxide dismutase [Cu-Zn] (SODCC) / ... 98 3e-21
At2g28190.1 68415.m03423 superoxide dismutase [Cu-Zn], chloropla... 94 6e-20
At1g12520.1 68414.m01449 superoxide dismutase copper chaperone, ... 60 9e-10
At1g10970.1 68414.m01259 metal transporter, putative (ZIP4) simi... 31 0.38
At5g08470.1 68418.m00999 peroxisome biogenesis protein (PEX1) id... 30 0.88
At4g38660.1 68417.m05473 thaumatin, putative similar to thaumati... 30 1.2
At3g54230.1 68416.m05994 zinc finger protein-related / D111/G-pa... 29 1.5
At3g11870.1 68416.m01455 protein kinase-related contains eukaryo... 29 1.5
At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)... 29 2.0
At5g61350.1 68418.m07698 protein kinase family protein contains ... 28 4.7
At1g15780.1 68414.m01893 expressed protein 28 4.7
At5g39380.1 68418.m04771 calmodulin-binding protein-related has ... 27 6.2
At4g24800.1 68417.m03552 MA3 domain-containing protein similar t... 27 6.2
At5g19740.1 68418.m02347 peptidase M28 family protein ileal pept... 27 8.2
At4g07670.1 68417.m01203 protease-associated (PA) domain-contain... 27 8.2
At3g24180.1 68416.m03035 expressed protein contains Pfam domain ... 27 8.2
At2g30690.1 68415.m03742 expressed protein contains Pfam profile... 27 8.2
At1g60960.1 68414.m06862 metal transporter, putative (IRT3) iden... 27 8.2
>At5g18100.1 68418.m02125 superoxide dismutase [Cu-Zn] / copper/zinc
superoxide dismutase (CSD3) identical to copper/zinc
superoxide dismutase GI:3273755
Length = 164
Score = 104 bits (249), Expect = 4e-23
Identities = 48/101 (47%), Positives = 64/101 (63%), Gaps = 1/101 (0%)
Frame = +1
Query: 73 RAIAHLVGEN-IRGNITFTRLPDGKVHVEGSIVGLPPGHYGFHVHEKGDISGGCGSTGSH 249
RA+A + G+N +RG + F + G HV G I GL PG +GFH+H GD + GC STG H
Sbjct: 9 RAVALIAGDNNVRGCLQFVQDISGTTHVTGKISGLSPGFHGFHIHSFGDTTNGCISTGPH 68
Query: 250 FNPENKEHGHPSDENRHVGDLGNAEFDQNYSSKIDMIDPHL 372
FNP N+ HG P++E RH GDLGN N ++I + D H+
Sbjct: 69 FNPLNRVHGPPNEEERHAGDLGNILAGSNGVAEILIKDKHI 109
Score = 58.8 bits (136), Expect = 2e-09
Identities = 25/45 (55%), Positives = 32/45 (71%)
Frame = +2
Query: 380 TGAHGILGRAVVLHERADDFGRTDHPDSRKTGNAGGRVACGVIGI 514
+G + ILGRAVV+H DD G+ H S+ TGNAG RV CG+IG+
Sbjct: 112 SGQYSILGRAVVVHADPDDLGKGGHKLSKSTGNAGSRVGCGIIGL 156
>At1g08830.1 68414.m00983 superoxide dismutase [Cu-Zn] (SODCC) /
copper/zinc superoxide dismutase (CSD1) identical to
SWISS-PROT: P24704
Length = 152
Score = 98.3 bits (234), Expect = 3e-21
Identities = 42/74 (56%), Positives = 49/74 (66%)
Frame = +1
Query: 97 ENIRGNITFTRLPDGKVHVEGSIVGLPPGHYGFHVHEKGDISGGCGSTGSHFNPENKEHG 276
E + G I FT+ DG V G++ GL PG +GFHVH GD + GC STG HFNP+ K HG
Sbjct: 12 EGVTGTIFFTQEGDGVTTVSGTVSGLKPGLHGFHVHALGDTTNGCMSTGPHFNPDGKTHG 71
Query: 277 HPSDENRHVGDLGN 318
P D NRH GDLGN
Sbjct: 72 APEDANRHAGDLGN 85
Score = 60.9 bits (141), Expect = 5e-10
Identities = 27/45 (60%), Positives = 33/45 (73%)
Frame = +2
Query: 380 TGAHGILGRAVVLHERADDFGRTDHPDSRKTGNAGGRVACGVIGI 514
TG + I+GRAVV+H DD G+ H S TGNAGGRVACG+IG+
Sbjct: 106 TGPNSIVGRAVVVHADPDDLGKGGHELSLATGNAGGRVACGIIGL 150
>At2g28190.1 68415.m03423 superoxide dismutase [Cu-Zn], chloroplast
(SODCP) / copper/zinc superoxide dismutase (CSD2)
identical to GP:3273753:AF061519
Length = 216
Score = 93.9 bits (223), Expect = 6e-20
Identities = 45/102 (44%), Positives = 60/102 (58%), Gaps = 1/102 (0%)
Frame = +1
Query: 61 SQPTRAIAHLVG-ENIRGNITFTRLPDGKVHVEGSIVGLPPGHYGFHVHEKGDISGGCGS 237
S +A+A L G ++ G +T T+ G V I GL PG +GFH+HE GD + GC S
Sbjct: 62 SAAKKAVAVLKGTSDVEGVVTLTQDDSGPTTVNVRITGLTPGPHGFHLHEFGDTTNGCIS 121
Query: 238 TGSHFNPENKEHGHPSDENRHVGDLGNAEFDQNYSSKIDMID 363
TG HFNP N HG P DE RH GDLGN + + ++ ++D
Sbjct: 122 TGPHFNPNNMTHGAPEDECRHAGDLGNINANADGVAETTIVD 163
Score = 60.1 bits (139), Expect = 9e-10
Identities = 26/45 (57%), Positives = 33/45 (73%)
Frame = +2
Query: 380 TGAHGILGRAVVLHERADDFGRTDHPDSRKTGNAGGRVACGVIGI 514
TG + ++GRA V+HE DD G+ H S TGNAGGR+ACGVIG+
Sbjct: 169 TGPNSVVGRAFVVHELKDDLGKGGHELSLTTGNAGGRLACGVIGL 213
>At1g12520.1 68414.m01449 superoxide dismutase copper chaperone,
putative similar to copper chaperone for superoxide
dismutase [Homo sapiens] gi|2431868|gb|AAC51764
Length = 254
Score = 60.1 bits (139), Expect = 9e-10
Identities = 41/127 (32%), Positives = 64/127 (50%), Gaps = 10/127 (7%)
Frame = +1
Query: 76 AIAHLVGENIRGNITFTRLPDGKVHVEGSIVGLPPGHYGFHVHEKGDISGGCGSTGSHFN 255
A+A G +I G + F ++ +E + GL PG + + ++E GD++ G STGS +N
Sbjct: 98 AVAEFKGPDIFGVVRFAQVSMELARIEANFTGLSPGTHSWCINEYGDLTNGAASTGSLYN 157
Query: 256 PENKEHGHPSDENRHVGDLGNAEFDQN----YSSK------IDMIDPHLGIYRSTRDSRQ 405
P + G +GDLG E D+N YS K D+I + +Y+ T D++
Sbjct: 158 PFQDQTG-----TEPLGDLGTLEADKNGEAFYSGKKEKLKVADLIGRAVVVYK-TDDNKS 211
Query: 406 GCGSTRA 426
G G T A
Sbjct: 212 GPGLTAA 218
>At1g10970.1 68414.m01259 metal transporter, putative (ZIP4) similar
to Zn and Cd transporter ZNT1 [Thlaspi caerulescens]
gi|7381054|gb|AAF61374; member of the Zinc (Zn2+)-Iron
(Fe2+) permease (ZIP) family, PMID:11500563
Length = 408
Score = 31.5 bits (68), Expect = 0.38
Identities = 24/78 (30%), Positives = 33/78 (42%), Gaps = 2/78 (2%)
Frame = +1
Query: 76 AIAHLVGENIRGNITFTRLPDGKVHVEGSIVGLPPGHYGFHVHEKGDISGGCGSTGSHFN 255
A+ +VGE + N F G +H IVG+ H H H + G C +H +
Sbjct: 182 AVVPVVGERVTDNKVFGEEDGGGIH----IVGI-RAHAAHHRHSHSNSHGTCDG-HAHGH 235
Query: 256 PENKEHGHPSDEN--RHV 303
HG+ EN RHV
Sbjct: 236 SHGHMHGNSDVENGARHV 253
>At5g08470.1 68418.m00999 peroxisome biogenesis protein (PEX1)
identical to peroxisome biogenesis protein PEX1
[Arabidopsis thaliana] gi|12006272|gb|AAG44817; contains
Pfam profile PF00004: ATPase, AAA family; identical to
cDNA peroxisome biogenesis protein PEX1 (PEX1) mRNA,
partial cds GI:12006271
Length = 1130
Score = 30.3 bits (65), Expect = 0.88
Identities = 16/49 (32%), Positives = 25/49 (51%), Gaps = 3/49 (6%)
Frame = -3
Query: 531 REMFYSIPMTPQATRPPALPVFLESG---WSVRPKSSARSCRTTALPRI 394
R++ +++ T + PP LPV L SG WSV S+ S + R+
Sbjct: 34 RQLLHALQSTSSSPLPPLLPVELRSGDRRWSVAWSGSSSSSSAIEIARV 82
>At4g38660.1 68417.m05473 thaumatin, putative similar to
thaumatin-like protein [Arabidopsis thaliana]
GI:2435406, thaumatin-like protein precursor [Pyrus
pyrifolia] GI:3241854; contains Pfam profile PF00314:
Thaumatin family
Length = 345
Score = 29.9 bits (64), Expect = 1.2
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 5/47 (10%)
Frame = -1
Query: 185 WPG-----GSPTIDPSTWTLPSGSRVNVILPLMFSPTRWAIARVGCE 60
WPG GSPT+ + + LP G+ ++ P +S W AR GC+
Sbjct: 43 WPGILSNAGSPTLSTTGFELPKGTSRSLQAPTGWSGRFW--ARTGCK 87
>At3g54230.1 68416.m05994 zinc finger protein-related / D111/G-patch
domain-containing protein / RNA recognition motif
(RRM)-containing protein KIAA0122 gene , Homo sapiens,
EMBL:HSDKG02; contains Pfam profiles PF00076: RNA
recognition motif. (a.k.a. RRM, RBD, or RNP domain),
PF01585: G-patch domain, weak hit to PF00641: Zn-finger
in Ran binding protein and others
Length = 1105
Score = 29.5 bits (63), Expect = 1.5
Identities = 18/62 (29%), Positives = 24/62 (38%), Gaps = 2/62 (3%)
Frame = +1
Query: 160 SIVGLPPGHYGFHVHEKGDISGGCGSTGSHFNP-ENKEHGHPSDENRHVG-DLGNAEFDQ 333
S G GHY H HE+ GC + N +K + H D+ R D +D
Sbjct: 124 SEAGSRNGHYRDHEHERSSRYDGCDDYSCNDNNYRSKNYHHSRDDGREKDYDYTRRSYDS 183
Query: 334 NY 339
Y
Sbjct: 184 EY 185
>At3g11870.1 68416.m01455 protein kinase-related contains eukaryotic
protein kinase domain, INTERPRO:IPR000719
Length = 554
Score = 29.5 bits (63), Expect = 1.5
Identities = 14/32 (43%), Positives = 17/32 (53%)
Frame = +1
Query: 199 VHEKGDISGGCGSTGSHFNPENKEHGHPSDEN 294
V +K D SGG G N E+K PSD+N
Sbjct: 58 VEKKSDPSGGLGEENEKTNSESKVLSVPSDQN 89
>At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)
family protein similar to SP|P41972 Isoleucyl-tRNA
synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase) (IleRS)
{Staphylococcus aureus}; contains Pfam profile PF00133:
tRNA synthetases class I (I, L, M and V)
Length = 1093
Score = 29.1 bits (62), Expect = 2.0
Identities = 12/31 (38%), Positives = 16/31 (51%)
Frame = -2
Query: 400 ENPVCSGKCRDAGRSYQSCWSSSGQIPRYQD 308
EN V G R G + CW+ SGQ+ + D
Sbjct: 1035 ENKVWIGVSRAEGSKCERCWNYSGQVGSFSD 1065
>At5g61350.1 68418.m07698 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 842
Score = 27.9 bits (59), Expect = 4.7
Identities = 19/46 (41%), Positives = 23/46 (50%), Gaps = 7/46 (15%)
Frame = -1
Query: 224 PDMSPFSWTW---NP*--WPGGSP--TIDPSTWTLPSGSRVNVILP 108
P + P S TW P +P GS T+DPST T P G +I P
Sbjct: 228 PKIDPLSRTWLSDKPYNTFPEGSRNVTVDPSTITYPDGGATALIAP 273
>At1g15780.1 68414.m01893 expressed protein
Length = 1335
Score = 27.9 bits (59), Expect = 4.7
Identities = 12/34 (35%), Positives = 16/34 (47%), Gaps = 2/34 (5%)
Frame = -1
Query: 197 WNP*WPGGSPTIDPSTW--TLPSGSRVNVILPLM 102
W P P G P +D W LP SR ++ +M
Sbjct: 6 WRPSLPNGEPAMDTGDWRTQLPPDSRQKIVNKIM 39
>At5g39380.1 68418.m04771 calmodulin-binding protein-related has
weak similarity to calmodulin-binding proteins
Length = 507
Score = 27.5 bits (58), Expect = 6.2
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = -3
Query: 510 PMTPQATRPPALPVFLESGWSVRPKSSARSCRTTALPRIPCAPVNAEMRVDHIN 349
P+TP+A+ P L V L S+R S ++ +++ PVN R D N
Sbjct: 241 PVTPRASLSPRLSVRLAGSSSLRKSQSLKAASSSSRQNQKPRPVN---RTDEFN 291
>At4g24800.1 68417.m03552 MA3 domain-containing protein similar to
programmed cell death 4 protein [Gallus gallus]
GI:12958564; contains Pfam profile PF02847: MA3 domain
Length = 702
Score = 27.5 bits (58), Expect = 6.2
Identities = 16/57 (28%), Positives = 23/57 (40%)
Frame = +1
Query: 139 GKVHVEGSIVGLPPGHYGFHVHEKGDISGGCGSTGSHFNPENKEHGHPSDENRHVGD 309
GK + V H G + K D GG G+ G + + H P+D N G+
Sbjct: 44 GKASGASNAVKHRRSHAGRSIRSKKDGGGGKGNWGKLIDTDGDYHIDPNDPNYDSGE 100
>At5g19740.1 68418.m02347 peptidase M28 family protein ileal
peptidase I100 - Rattus norvegicus, EMBL:AF009921;
contains Pfam profiles PF04389: Peptidase family M28,
PF02225: PA domain
Length = 681
Score = 27.1 bits (57), Expect = 8.2
Identities = 16/42 (38%), Positives = 19/42 (45%), Gaps = 1/42 (2%)
Frame = -1
Query: 179 GGSPTIDPSTWTLPSGSRVNVILPLMFSPTRWAIARV-GCER 57
GG S W PSG +V + + PT A V GCER
Sbjct: 206 GGDEWFPASKWMPPSGVQVGTVYNGLGDPTTPGWASVDGCER 247
>At4g07670.1 68417.m01203 protease-associated (PA) domain-containing
protein similar to PF02225: PA domain; similar to
N-acetylated-alpha-linked acidic dipeptidase II
(NAALADase II) (SP:Q9Y3Q) {Homo sapiens};similar to
Glutamate carboxypeptidase II (Membrane glutamate
carboxypeptidase) (mGCP) (N-acetylated-alpha-linked
acidic dipeptidase I) (NAALADase I)
(Pteroylpoly-gamma-glutamate
carboxypeptidase)(Folylpoly-gamma-glutamate
carboxypeptidase) (FGCP) (Folate hydrolase 1)
(Prostate-specific membrane antigen homolog) (SP|O77564)
{Sus scrofa}
Length = 280
Score = 27.1 bits (57), Expect = 8.2
Identities = 16/42 (38%), Positives = 19/42 (45%), Gaps = 1/42 (2%)
Frame = -1
Query: 179 GGSPTIDPSTWTLPSGSRVNVILPLMFSPTRWAIARV-GCER 57
GG S W PSG +V + + PT A V GCER
Sbjct: 71 GGDEWFPASKWMPPSGFQVGTVYNGLGDPTTPGWASVDGCER 112
>At3g24180.1 68416.m03035 expressed protein contains Pfam domain
PF04685: Protein of unknown function, DUF608
Length = 950
Score = 27.1 bits (57), Expect = 8.2
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 484 WTRRLRCHRNTVKHLSIS 537
W RRL CH N +K +I+
Sbjct: 40 WRRRLNCHANILKEFTIT 57
>At2g30690.1 68415.m03742 expressed protein contains Pfam profile
PF04576: Protein of unknown function, DUF593; expression
supported by MPSS
Length = 788
Score = 27.1 bits (57), Expect = 8.2
Identities = 15/51 (29%), Positives = 25/51 (49%)
Frame = +1
Query: 193 FHVHEKGDISGGCGSTGSHFNPENKEHGHPSDENRHVGDLGNAEFDQNYSS 345
FH + ++SG +G F + E+G S E R +++F QN +S
Sbjct: 371 FHAENEAEVSGSSSPSGGEFLSPSAENG-ASREIRIQEHDDSSDFSQNITS 420
>At1g60960.1 68414.m06862 metal transporter, putative (IRT3)
identical to putative metal transporter IRT3
[Arabidopsis thaliana] gi|17385796|gb|AAL38438; similar
to iron-regulated transporter 1 [Lycopersicon
esculentum] gi|9716481|gb|AAF97509; member of the Zinc
(Zn2+)-Iron (Fe2+) permease (ZIP) family, PMID:11500563
Length = 425
Score = 27.1 bits (57), Expect = 8.2
Identities = 21/81 (25%), Positives = 28/81 (34%), Gaps = 7/81 (8%)
Frame = +1
Query: 58 RSQPTRAIAHLVGENIRGNITFTRLPDGKVHVEGSIVGL-------PPGHYGFHVHEKGD 216
R Q + ++GE F G +H+ G PPGH H K D
Sbjct: 180 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 239
Query: 217 ISGGCGSTGSHFNPENKEHGH 279
I G +H + HGH
Sbjct: 240 I----GHAHAHGHGHGHGHGH 256
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,409,602
Number of Sequences: 28952
Number of extensions: 278470
Number of successful extensions: 822
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 792
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 822
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1023490624
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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