Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_M03
(503 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g25110.1 68417.m03612 latex-abundant family protein (AMC2) / ... 30 1.0
At1g36150.1 68414.m04494 protease inhibitor/seed storage/lipid t... 30 1.0
At5g51380.1 68418.m06370 F-box family protein contains Pfam PF00... 29 1.8
At5g23150.1 68418.m02707 PWWP domain-containing protein identica... 29 1.8
At5g58210.3 68418.m07288 hydroxyproline-rich glycoprotein family... 29 2.4
At5g58210.2 68418.m07287 hydroxyproline-rich glycoprotein family... 29 2.4
At5g58210.1 68418.m07286 hydroxyproline-rich glycoprotein family... 29 2.4
At1g79650.3 68414.m09289 DNA repair protein RAD23, putative simi... 29 2.4
At1g79650.2 68414.m09288 DNA repair protein RAD23, putative simi... 29 2.4
At1g79650.1 68414.m09287 DNA repair protein RAD23, putative simi... 29 2.4
At5g44220.1 68418.m05410 F-box family protein similar to unknown... 28 3.1
At1g23230.1 68414.m02906 expressed protein 28 3.1
At2g42390.1 68415.m05246 protein kinase C substrate, heavy chain... 28 4.1
At1g51660.1 68414.m05820 mitogen-activated protein kinase kinase... 27 5.4
At5g05500.1 68418.m00596 pollen Ole e 1 allergen and extensin fa... 27 7.2
At3g32400.1 68416.m04142 formin homology 2 domain-containing pro... 27 7.2
At4g18940.1 68417.m02791 cyclic phosphodiesterase, putative simi... 27 9.5
At4g18670.1 68417.m02762 leucine-rich repeat family protein / ex... 27 9.5
At1g49610.1 68414.m05562 F-box family protein contains F-box dom... 27 9.5
>At4g25110.1 68417.m03612 latex-abundant family protein (AMC2) /
caspase family protein contains similarity to
latex-abundant protein [Hevea brasiliensis] gb:AAD13216;
contains Pfam profile PF00656: ICE-like protease
(caspase) p20 domain
Length = 418
Score = 29.9 bits (64), Expect = 1.0
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -3
Query: 489 PAATTTSHALPAPVPPFSGQRAAV 418
PA++ +HA P P PP GQ+ AV
Sbjct: 96 PASSPFNHAPPGPPPPVHGQKRAV 119
>At1g36150.1 68414.m04494 protease inhibitor/seed storage/lipid
transfer protein (LTP) family protein low similarity to
glucoamylase S1/S2 [Precursor] from Saccharomyces
cerevisiae [SP|P08640], proteophosphoglycan from
Leishmania major [GI:5420387]; contains Pfam protease
inhibitor/seed storage/LTP family domain PF00234
Length = 256
Score = 29.9 bits (64), Expect = 1.0
Identities = 20/70 (28%), Positives = 30/70 (42%)
Frame = -3
Query: 501 SSEDPAATTTSHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSALTQNSRCLHLNSLH 322
S++ PA T +S A+ PP VS S P S+P RS+ + + +
Sbjct: 145 SAKSPAITPSSPAVSHSPPPVRHSSPPVSHSSPPVSHSSPPTSRSSPAVSHSSPVVAASS 204
Query: 321 PCSGMGSLTA 292
P + S TA
Sbjct: 205 PVKAVSSSTA 214
>At5g51380.1 68418.m06370 F-box family protein contains Pfam
PF00646: F-box domain; similar to F-box protein FBL2
(GI:6063090) [Homo sapiens]
Length = 479
Score = 29.1 bits (62), Expect = 1.8
Identities = 14/36 (38%), Positives = 19/36 (52%)
Frame = +2
Query: 176 CRNTKQQVGALFTRSNFVTLKYVTDNWGTDANGFKL 283
C N K+ + ALF + VT + D WG D + F L
Sbjct: 351 CLNDKEGMRALFKVCDGVTKVNIQDCWGLDDDSFSL 386
>At5g23150.1 68418.m02707 PWWP domain-containing protein identical to
cDNA putative transcription factor (HUA2) GI:4868119;
contains Pfam profile PF00855: PWWP domain
Length = 1392
Score = 29.1 bits (62), Expect = 1.8
Identities = 16/51 (31%), Positives = 21/51 (41%)
Frame = -3
Query: 471 SHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSALTQNSRCLHLNSLHP 319
S+ P P PP + +P W PS SA QN +H + HP
Sbjct: 1283 SYTHPYPFPPQRDDARRYRNEEP---WRIPSSGHSAENQNGAWIHGRNSHP 1330
>At5g58210.3 68418.m07288 hydroxyproline-rich glycoprotein family
protein
Length = 380
Score = 28.7 bits (61), Expect = 2.4
Identities = 17/40 (42%), Positives = 20/40 (50%)
Frame = -3
Query: 483 ATTTSHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSA 364
A T+SH PAPVP + A+S PS T SH A
Sbjct: 177 ADTSSHFSPAPVPIVEAE--ALSVVSPSVPADTSSHFSPA 214
>At5g58210.2 68418.m07287 hydroxyproline-rich glycoprotein family
protein
Length = 380
Score = 28.7 bits (61), Expect = 2.4
Identities = 17/40 (42%), Positives = 20/40 (50%)
Frame = -3
Query: 483 ATTTSHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSA 364
A T+SH PAPVP + A+S PS T SH A
Sbjct: 177 ADTSSHFSPAPVPIVEAE--ALSVVSPSVPADTSSHFSPA 214
>At5g58210.1 68418.m07286 hydroxyproline-rich glycoprotein family
protein
Length = 380
Score = 28.7 bits (61), Expect = 2.4
Identities = 17/40 (42%), Positives = 20/40 (50%)
Frame = -3
Query: 483 ATTTSHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSA 364
A T+SH PAPVP + A+S PS T SH A
Sbjct: 177 ADTSSHFSPAPVPIVEAE--ALSVVSPSVPADTSSHFSPA 214
>At1g79650.3 68414.m09289 DNA repair protein RAD23, putative similar
to DNA repair by nucleotide excision (NER) RAD23
protein, isoform II GI:1914685 from [Daucus carota];
contains Pfam profiles PF00240: Ubiquitin family,
PF00627: UBA/TS-N domain
Length = 351
Score = 28.7 bits (61), Expect = 2.4
Identities = 15/49 (30%), Positives = 23/49 (46%)
Frame = -3
Query: 501 SSEDPAATTTSHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSALTQ 355
SS PAA +T+ + P P P Q + +D Q ++ S+L Q
Sbjct: 102 SSTKPAAPSTTQSSPVPASPIPAQEQPAAQTDTYGQAASTLVSGSSLEQ 150
>At1g79650.2 68414.m09288 DNA repair protein RAD23, putative similar
to DNA repair by nucleotide excision (NER) RAD23
protein, isoform II GI:1914685 from [Daucus carota];
contains Pfam profiles PF00240: Ubiquitin family,
PF00627: UBA/TS-N domain
Length = 365
Score = 28.7 bits (61), Expect = 2.4
Identities = 15/49 (30%), Positives = 23/49 (46%)
Frame = -3
Query: 501 SSEDPAATTTSHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSALTQ 355
SS PAA +T+ + P P P Q + +D Q ++ S+L Q
Sbjct: 96 SSTKPAAPSTTQSSPVPASPIPAQEQPAAQTDTYGQAASTLVSGSSLEQ 144
>At1g79650.1 68414.m09287 DNA repair protein RAD23, putative similar
to DNA repair by nucleotide excision (NER) RAD23
protein, isoform II GI:1914685 from [Daucus carota];
contains Pfam profiles PF00240: Ubiquitin family,
PF00627: UBA/TS-N domain
Length = 371
Score = 28.7 bits (61), Expect = 2.4
Identities = 15/49 (30%), Positives = 23/49 (46%)
Frame = -3
Query: 501 SSEDPAATTTSHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSALTQ 355
SS PAA +T+ + P P P Q + +D Q ++ S+L Q
Sbjct: 102 SSTKPAAPSTTQSSPVPASPIPAQEQPAAQTDTYGQAASTLVSGSSLEQ 150
>At5g44220.1 68418.m05410 F-box family protein similar to unknown
protein (pir||T06086)
Length = 295
Score = 28.3 bits (60), Expect = 3.1
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +2
Query: 308 IPEHGCKEFRCKQREFCV 361
I EH CK RCK+R C+
Sbjct: 263 ITEHNCKLLRCKKRHPCI 280
>At1g23230.1 68414.m02906 expressed protein
Length = 1615
Score = 28.3 bits (60), Expect = 3.1
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Frame = -3
Query: 408 DPSAQWSTPSHMRSALTQNSRCLH-LNSLHPCSGMGSLTAVITNLKPFASVPQLSVTYLS 232
DP+ W+ + +A+ LH S P + A+ITNL+P SV L V +
Sbjct: 1438 DPT--WAAQDNTSTAIGNIVALLHAFFSNLPQEWLDGTNAIITNLRPVTSVAMLRVVFRI 1495
Query: 231 VTKLL 217
+ LL
Sbjct: 1496 MGPLL 1500
>At2g42390.1 68415.m05246 protein kinase C substrate, heavy
chain-related similar to Protein kinase C substrate, 80
kDa protein, heavy chain (PKCSH) (80K-H protein)
(Swiss-Prot:P14314) [Homo sapiens]; contains 1
transmembrane domain;
Length = 212
Score = 27.9 bits (59), Expect = 4.1
Identities = 13/37 (35%), Positives = 18/37 (48%)
Frame = +2
Query: 335 RCKQREFCVSADLMCDGVDHCADGSDEDTAALCPENG 445
+CK + D + D C DG+DE + CP NG
Sbjct: 42 KCKDGSKSFTRDRLNDNFCDCLDGTDEPGTSACP-NG 77
>At1g51660.1 68414.m05820 mitogen-activated protein kinase kinase
(MAPKK), putative (MKK4) identical to MAP kinase kinase
4 [Arabidopsis thaliana] gi|3219271|dbj|BAA28830
gi_13265419
Length = 366
Score = 27.5 bits (58), Expect = 5.4
Identities = 14/31 (45%), Positives = 17/31 (54%)
Frame = -3
Query: 471 SHALPAPVPPFSGQRAAVSSSDPSAQWSTPS 379
S A+P P+PP SG S S PS+ S S
Sbjct: 34 SLAVPLPLPPTSGGSGGSSGSAPSSGGSASS 64
>At5g05500.1 68418.m00596 pollen Ole e 1 allergen and extensin
family protein contains Pfam domain, PF01190: Pollen
proteins Ole e I family
Length = 183
Score = 27.1 bits (57), Expect = 7.2
Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 4/78 (5%)
Frame = +2
Query: 137 GAHATTPAKVDLSCRNTKQQVGALFTRSNFVTLKYVTDNWG---TDANGFKLVITAVKDP 307
GA A AK+ + C+N +QQV +F + + TD++G + GFK+ + P
Sbjct: 64 GAEAIAGAKISIICKNHRQQV-------SFYKV-FRTDSYGHFYGELKGFKMTPHFLDHP 115
Query: 308 IPEHGCK-EFRCKQREFC 358
+ H C+ + RE C
Sbjct: 116 L--HSCRAKLVSSPREDC 131
>At3g32400.1 68416.m04142 formin homology 2 domain-containing
protein / FH2 domain-containing protein common family
members: At2g43800, At3g25500, At5g48360, At4g15200,
At3g05470, At3g07540, At5g07780, At5g07650 [Arabidopsis
thaliana];
Length = 488
Score = 27.1 bits (57), Expect = 7.2
Identities = 20/69 (28%), Positives = 30/69 (43%)
Frame = -3
Query: 474 TSHALPAPVPPFSGQRAAVSSSDPSAQWSTPSHMRSALTQNSRCLHLNSLHPCSGMGSLT 295
+S LP P+PP ++ +++ P P H S + + L L S H G G
Sbjct: 29 SSSPLPPPLPP---KKLLATTNTPPPP-PPPLHSNSQMGAPTSSLVLKSPHNLKGQGQTR 84
Query: 294 AVITNLKPF 268
NLKP+
Sbjct: 85 K--ANLKPY 91
>At4g18940.1 68417.m02791 cyclic phosphodiesterase, putative similar
to cyclic phosphodiesterase [Arabidopsis thaliana]
gi|2065013|emb|CAA72363
Length = 196
Score = 26.6 bits (56), Expect = 9.5
Identities = 15/52 (28%), Positives = 26/52 (50%), Gaps = 3/52 (5%)
Frame = -3
Query: 369 SALTQNSRCLHLNSLHPCSGMGSLTAVITNLKPFAS---VPQLSVTYLSVTK 223
SA T +CL+++ H M + + + KPF VP +S+ Y +T+
Sbjct: 96 SAGTSYFQCLYVSLRHTVEVMNAAGHFMAHFKPFTGKFYVPHMSILYGDLTE 147
>At4g18670.1 68417.m02762 leucine-rich repeat family protein /
extensin family protein similar to extensin-like protein
[Lycopersicon esculentum] gi|5917664|gb|AAD55979;
contains leucine-rich repeats, Pfam:PF00560; contains
proline rich extensin domains, INTERPRO:IPR002965
Length = 839
Score = 26.6 bits (56), Expect = 9.5
Identities = 13/33 (39%), Positives = 19/33 (57%), Gaps = 3/33 (9%)
Frame = -1
Query: 485 LLPPPATHYQHQYRHSQDRE---QQYLHLIRRR 396
LL P +H HQY H + R ++ HL+RR+
Sbjct: 632 LLLPQLSHLPHQYLHHRLRHILPSRHRHLLRRK 664
>At1g49610.1 68414.m05562 F-box family protein contains F-box domain
Pfam:PF00646
Length = 354
Score = 26.6 bits (56), Expect = 9.5
Identities = 17/60 (28%), Positives = 24/60 (40%), Gaps = 3/60 (5%)
Frame = +2
Query: 8 LWSKNERNL---DCTVTFQTHSILQRFMLHFDLLQLDCNDHLYVYDGAHATTPAKVDLSC 178
LW N NL D + F ++ L FD D D+LY+ + AK+ C
Sbjct: 98 LWITNGINLPYIDGWIKFAMSRNVENLFLSFDFRLYDVPDYLYINSSVKQLSIAKILSGC 157
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,252,204
Number of Sequences: 28952
Number of extensions: 187848
Number of successful extensions: 757
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 706
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 754
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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