Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_L11
(392 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein L36|Schizosacc... 85 5e-18
SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein L36|Sch... 85 5e-18
SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gc... 28 0.45
SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor |Schizo... 26 1.8
SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase Ssp2|Schiz... 26 2.4
SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces pombe... 25 5.5
SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly sc... 24 7.3
SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa... 24 7.3
SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor Raf1|S... 24 7.3
SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pm... 24 7.3
SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering compo... 24 9.7
SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1 |S... 24 9.7
SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1 |Schizosaccharo... 24 9.7
SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr 3|... 24 9.7
>SPCC970.05 |rpl3601|rpl36-1|60S ribosomal protein
L36|Schizosaccharomyces pombe|chr 3|||Manual
Length = 99
Score = 84.6 bits (200), Expect = 5e-18
Identities = 43/84 (51%), Positives = 58/84 (69%)
Frame = +3
Query: 87 KGITDKAIKIRPARLKGIQTKHSKFVRDLVREVVGHAQYEKRAMELLKVSKDKRALKFLK 266
K +T + + RP+R KG +K + FVR +VREV G A YE+R MEL++ S+DKRA K K
Sbjct: 13 KVLTKRQLPERPSRRKGQLSKRTSFVRSIVREVAGFAPYERRVMELIRNSQDKRARKLAK 72
Query: 267 RRLGTHIRAKRKREELSNVLTQMR 338
+RLGT RAK K EEL++V+ R
Sbjct: 73 KRLGTLKRAKGKIEELTSVIQSSR 96
>SPBC405.07 |rpl3602|rpl36-2, rpl36|60S ribosomal protein
L36|Schizosaccharomyces pombe|chr 2|||Manual
Length = 99
Score = 84.6 bits (200), Expect = 5e-18
Identities = 43/84 (51%), Positives = 58/84 (69%)
Frame = +3
Query: 87 KGITDKAIKIRPARLKGIQTKHSKFVRDLVREVVGHAQYEKRAMELLKVSKDKRALKFLK 266
K +T + + RP+R KG +K + FVR +VREV G A YE+R MEL++ S+DKRA K K
Sbjct: 13 KTLTKRQLPERPSRRKGHLSKRTAFVRSIVREVAGFAPYERRVMELIRNSQDKRARKLAK 72
Query: 267 RRLGTHIRAKRKREELSNVLTQMR 338
+RLGT RAK K EEL++V+ R
Sbjct: 73 KRLGTLKRAKGKIEELTSVIQSSR 96
>SPBC36B7.09 |gcn2|ppk28, ppk28, SPBP18G5.01|eIF2 alpha kinase Gcn2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1576
Score = 28.3 bits (60), Expect = 0.45
Identities = 19/53 (35%), Positives = 28/53 (52%), Gaps = 1/53 (1%)
Frame = +3
Query: 183 VVGHAQYEKRAMELLKVSKDKRALKFLKRRLGTHIRAK-RKREELSNVLTQMR 338
++ HA ++ L+VSKD KRR+ THI + +R LS V Q+R
Sbjct: 1119 LINHADILSSILDYLQVSKD-------KRRMATHILGQINQRLTLSQVRNQLR 1164
>SPBC3E7.12c |chr1|cfh4|chitin synthase regulatory factor
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 456
Score = 26.2 bits (55), Expect = 1.8
Identities = 8/22 (36%), Positives = 17/22 (77%)
Frame = -2
Query: 244 LSFDTLSNSIALFSYCACPTTS 179
+S+D+LS+ +++ S+ CP T+
Sbjct: 79 MSYDSLSDDVSVLSFLDCPLTT 100
>SPCC74.03c |ssp2|ucp9|serine/threonine protein kinase
Ssp2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 576
Score = 25.8 bits (54), Expect = 2.4
Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 4/48 (8%)
Frame = +3
Query: 171 LVREVVGHAQYEKRAMELLKVSKDKRALKFLKRRL----GTHIRAKRK 302
++RE +G + K + ++ K ALKF+ R+L H+R +R+
Sbjct: 35 IIRETLGEGSFGKVKLATHYKTQQKVALKFISRQLLKKSDMHMRVERE 82
>SPBC26H8.12 |||cytochrome c heme lyase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 377
Score = 24.6 bits (51), Expect = 5.5
Identities = 8/20 (40%), Positives = 11/20 (55%)
Frame = +2
Query: 35 RNWSAKRPQNDKDIRWQKGY 94
+NW PQ + W+KGY
Sbjct: 219 KNWEYPSPQQMYNAMWRKGY 238
>SPAC227.13c |isu1||mitochondrial iron-sulfur cluster assembly
scaffold protein Isu1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 192
Score = 24.2 bits (50), Expect = 7.3
Identities = 12/35 (34%), Positives = 18/35 (51%)
Frame = +3
Query: 117 RPARLKGIQTKHSKFVRDLVREVVGHAQYEKRAME 221
RPA L+ ++T SKFV + V Y K ++
Sbjct: 27 RPANLQFLKTNSSKFVPQVTAN-VSRRMYHKNVLD 60
>SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 941
Score = 24.2 bits (50), Expect = 7.3
Identities = 12/28 (42%), Positives = 18/28 (64%)
Frame = -2
Query: 232 TLSNSIALFSYCACPTTSRTKSRTNLEC 149
T+++S LF+ A T+ TKS N+EC
Sbjct: 850 TINHSRTLFATHAHQLTNLTKSFKNVEC 877
>SPCC613.12c |raf1|dos1, cmc1, clr8|Rik1-associated factor
Raf1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 638
Score = 24.2 bits (50), Expect = 7.3
Identities = 10/26 (38%), Positives = 16/26 (61%)
Frame = -3
Query: 339 SSSVLVRC*ALHASSSRGCVCRGDAS 262
S V++ + H S++RGC+ DAS
Sbjct: 406 SDKVIIGYDSFHTSNNRGCLALFDAS 431
>SPAPB2B4.04c ||pmc1, pmc1|P-type ATPase, calcium transporting Pmc1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1292
Score = 24.2 bits (50), Expect = 7.3
Identities = 14/31 (45%), Positives = 16/31 (51%), Gaps = 2/31 (6%)
Frame = -1
Query: 254 KGALIFRYFEQFHCPL--LILCVPNNFTNQI 168
KG I F PL LI CVPNNF ++
Sbjct: 1117 KGWAISIVFGVISIPLGALIRCVPNNFLRKV 1147
>SPAC17G6.08 |pep7|vac1|prevacuole/endosomal FYVE tethering
component Pep7 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 536
Score = 23.8 bits (49), Expect = 9.7
Identities = 21/94 (22%), Positives = 44/94 (46%), Gaps = 2/94 (2%)
Frame = +3
Query: 60 KTTKISAGKKGITDKAIKIR--PARLKGIQTKHSKFVRDLVREVVGHAQYEKRAMELLKV 233
K ++ K+ + + ++++ P L+ Q S+ R+L ++ Q EK+ EL++
Sbjct: 424 KQNVVNEAKRYLQETILRLQAIPYHLQVGQAWTSESERELEKK---KEQVEKKQEELMQT 480
Query: 234 SKDKRALKFLKRRLGTHIRAKRKREELSNVLTQM 335
FL + +AKRK E+ +L+ +
Sbjct: 481 RIVLEEQVFLVENMIEDAKAKRKFSEVETLLSSL 514
>SPBC4F6.06 |kin1||microtubule affinity-regulating kinase Kin1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 891
Score = 23.8 bits (49), Expect = 9.7
Identities = 20/63 (31%), Positives = 31/63 (49%), Gaps = 7/63 (11%)
Frame = +3
Query: 54 GHKTTKISAGKKGITDKAIKIRPARLKGI------QTKHSKFV-RDLVREVVGHAQYEKR 212
G+ K S TDK I+P LKG+ TK ++ + RDL+R V+G E +
Sbjct: 730 GNPVDKNSTSPSKSTDKLDPIKPVFLKGLFSVSTTSTKSTESIQRDLIR-VMGMLDIEYK 788
Query: 213 AME 221
++
Sbjct: 789 EIK 791
>SPAPB1A10.06c |||ATP-dependent RNA helicase Dhr1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1183
Score = 23.8 bits (49), Expect = 9.7
Identities = 12/39 (30%), Positives = 26/39 (66%)
Frame = +3
Query: 219 ELLKVSKDKRALKFLKRRLGTHIRAKRKREELSNVLTQM 335
+LLK ++ + K K+RL +I K K+EE+++++ ++
Sbjct: 75 QLLKENEGSISSK-KKKRLDKYIENKLKKEEVASLIAKL 112
>SPCC794.12c |mae2||malic enzyme|Schizosaccharomyces pombe|chr
3|||Manual
Length = 565
Score = 23.8 bits (49), Expect = 9.7
Identities = 11/52 (21%), Positives = 22/52 (42%)
Frame = +3
Query: 45 LRKGHKTTKISAGKKGITDKAIKIRPARLKGIQTKHSKFVRDLVREVVGHAQ 200
L+K ++ +G + ++P L G + KF +RE+ H +
Sbjct: 359 LKKDSDFKEVPSGDINLESAIALVKPTILLGCSGQPGKFTEKAIREMSKHVE 410
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,530,743
Number of Sequences: 5004
Number of extensions: 29047
Number of successful extensions: 104
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 95
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 104
length of database: 2,362,478
effective HSP length: 66
effective length of database: 2,032,214
effective search space used: 130061696
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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