SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_L01
         (477 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces...   175   4e-45
SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog 1|Schizosa...    32   0.052
SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual    28   0.64 
SPAC767.01c |vps1|SPAC9G1.14c|dynamin family protein Vps1|Schizo...    27   1.9  
SPBC17A3.03c |||phosphoprotein phosphatase |Schizosaccharomyces ...    26   2.6  
SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3 Rfp1|Schizo...    25   5.9  
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo...    25   5.9  
SPBC530.02 |||membrane transporter|Schizosaccharomyces pombe|chr...    25   7.9  
SPCC74.04 |||amino acid permease, unknown 15|Schizosaccharomyces...    25   7.9  
SPAC513.02 |||phosphoglycerate mutase family|Schizosaccharomyces...    25   7.9  

>SPAC18G6.14c |rps7||40S ribosomal protein S7|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 195

 Score =  175 bits (425), Expect = 4e-45
 Identities = 81/134 (60%), Positives = 103/134 (76%)
 Frame = +2

Query: 8   KKSIIIYVPMPKLKAFQKIQIRLVRELEKKFSGKHVVFVGDRKILPKPSHKTRVANKQKR 187
           KK+I+++VP P LKAF K Q RL RELEKKF+ +HV+F+  R+ILPKP  K+RV   QKR
Sbjct: 57  KKAIVVFVPQPLLKAFHKCQARLTRELEKKFADRHVIFIAQRRILPKPGRKSRVT--QKR 114

Query: 188 PRSRTLTSVYDAILEDLVFPAEIVGKRIRVKLDGSQLIKVHLDXNQQTTIEHKVDTFQSV 367
           PRSRTLT+V++AILED+VFP EI+GKR R   DG + IKV LD     T+++K+ +F SV
Sbjct: 115 PRSRTLTAVHNAILEDIVFPTEIIGKRTRQATDGRKTIKVFLDNRDANTVDYKLGSFSSV 174

Query: 368 YKKLTGREVTFEFP 409
           Y KLTG+ VTFEFP
Sbjct: 175 YHKLTGKNVTFEFP 188


>SPAC13F5.01c |msh1|SPAC23C11.18c|MutS protein homolog
           1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 941

 Score = 31.9 bits (69), Expect = 0.052
 Identities = 21/72 (29%), Positives = 39/72 (54%), Gaps = 1/72 (1%)
 Frame = +2

Query: 197 RTLTSVYDAILEDLVFPAEIVGKRIRVKLDGSQLIKVHLDXNQQTTIEHKVDTF-QSVYK 373
           ++L + YD + EDL   ++ +GK+  ++   ++L  VHL  +   TIE  +  F Q+V  
Sbjct: 565 QSLFASYDKLQEDL---SKRLGKKATLRKSPAKLYYVHLKLSGNETIERFIKKFTQAVLF 621

Query: 374 KLTGREVTFEFP 409
           + T    +F+ P
Sbjct: 622 QSTKSTASFQLP 633


>SPCC5E4.04 |cut1||separase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 1828

 Score = 28.3 bits (60), Expect = 0.64
 Identities = 23/81 (28%), Positives = 35/81 (43%), Gaps = 1/81 (1%)
 Frame = +2

Query: 110  HVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKRIRVKLDG 289
            H V V D+ +   P       N+Q   R  +L+ + D + +  V   E V  R   K  G
Sbjct: 1594 HTVLVLDKSVHQFPWESLPCLNRQSVSRVPSLSILRDILSQSFVVNGEYVEVR---KEAG 1650

Query: 290  SQLIKVHLD-XNQQTTIEHKV 349
            S ++   LD  + Q   EHK+
Sbjct: 1651 SYILNPSLDLKHTQEMFEHKL 1671


>SPAC767.01c |vps1|SPAC9G1.14c|dynamin family protein
           Vps1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 678

 Score = 26.6 bits (56), Expect = 1.9
 Identities = 13/31 (41%), Positives = 22/31 (70%)
 Frame = +2

Query: 23  IYVPMPKLKAFQKIQIRLVRELEKKFSGKHV 115
           +++P  K   F+KI+  +VRE E+K +GK+V
Sbjct: 101 LHLPGQKFFEFEKIREEIVRETEEK-TGKNV 130


>SPBC17A3.03c |||phosphoprotein phosphatase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 263

 Score = 26.2 bits (55), Expect = 2.6
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 4/49 (8%)
 Frame = -3

Query: 307 HFDEL*AIELHSDA--FANN--LGREYQIFQYGVIYRSQCPGTGPFLFV 173
           H D +  ++  +DA  F+N+  +   + +   G+IYRS CP    F F+
Sbjct: 36  HKDGIKVVDTSNDASTFSNSPLVPDNFGVVYPGIIYRSACPRASNFNFL 84


>SPAC19A8.10 |rfp1|mug140|ubiquitin-protein ligase E3
           Rfp1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 254

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 13/42 (30%), Positives = 22/42 (52%)
 Frame = +2

Query: 143 PKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIVGKR 268
           P  S + R  N+++  RSR   S + + LED+++    V  R
Sbjct: 49  PVLSPRRRRMNRRRNERSRNFPSNHLSYLEDMIYLGPQVSTR 90


>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharomyces
            pombe|chr 1|||Manual
          Length = 1811

 Score = 25.0 bits (52), Expect = 5.9
 Identities = 14/38 (36%), Positives = 17/38 (44%)
 Frame = -1

Query: 336  IVVCWFXSRCTLMSCEPSSFTLMRLPTISAGNTRSSNM 223
            I V W    C+L    P  F+L +L  IS  N R   M
Sbjct: 1173 IEVSWEEIECSLELSNPRLFSLQKLVEISYYNMRRIRM 1210


>SPBC530.02 |||membrane transporter|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 541

 Score = 24.6 bits (51), Expect = 7.9
 Identities = 11/32 (34%), Positives = 15/32 (46%)
 Frame = -1

Query: 99  NFFSNSRTSLIWIFWNAFSLGIGT*MIIDFFM 4
           +F   S     W+FW    +G    +II FFM
Sbjct: 245 DFICQSYLGWCWVFWINMIMGAVVIVIIFFFM 276


>SPCC74.04 |||amino acid permease, unknown 15|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 557

 Score = 24.6 bits (51), Expect = 7.9
 Identities = 10/31 (32%), Positives = 14/31 (45%)
 Frame = -2

Query: 227 IWRHIQKSVSWNGAFSVCWLHVSCGWAWAGS 135
           +W HI+    W   F++  L   CG  W  S
Sbjct: 247 VWSHIENYTDWPDGFAI--LMSFCGVIWTMS 275


>SPAC513.02 |||phosphoglycerate mutase family|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 216

 Score = 24.6 bits (51), Expect = 7.9
 Identities = 18/66 (27%), Positives = 28/66 (42%)
 Frame = +2

Query: 80  RELEKKFSGKHVVFVGDRKILPKPSHKTRVANKQKRPRSRTLTSVYDAILEDLVFPAEIV 259
           ++LEK+F G       +  + PK     +   K    RSR L   + A + + VF     
Sbjct: 108 KDLEKQFPGYDYTACHEDPVFPKKEKIYKADYKTSIQRSRVLAEFF-AKVPEKVFAVVTH 166

Query: 260 GKRIRV 277
           G  IR+
Sbjct: 167 GVDIRL 172


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,037,473
Number of Sequences: 5004
Number of extensions: 41866
Number of successful extensions: 130
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 125
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 129
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 184476110
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -