Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_J23
(472 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) simi... 97 7e-21
At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S ... 92 2e-19
At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A) 76 1e-14
At1g61900.2 68414.m06984 expressed protein contains similarity t... 29 1.6
At1g61900.1 68414.m06983 expressed protein contains similarity t... 29 1.6
At2g18850.1 68415.m02196 SET domain-containing protein low simil... 28 2.8
At5g11350.1 68418.m01325 endonuclease/exonuclease/phosphatase fa... 28 3.7
At3g28360.1 68416.m03544 ABC transporter family protein similar ... 28 3.7
At3g59820.1 68416.m06675 calcium-binding mitochondrial protein-r... 27 6.4
At3g28380.1 68416.m03546 P-glycoprotein, putative similar to P-g... 27 6.4
At3g28415.1 68416.m03551 P-glycoprotein, putative contains ATP-b... 27 8.5
At3g28390.1 68416.m03547 P-glycoprotein, putative similar to P-g... 27 8.5
>At3g05590.1 68416.m00621 60S ribosomal protein L18 (RPL18B) similar
to GB:P42791
Length = 187
Score = 96.7 bits (230), Expect = 7e-21
Identities = 50/107 (46%), Positives = 69/107 (64%)
Frame = +2
Query: 23 RKVRRTEVKSQDVXXXXXXXXXXXXXXXTNAKFNQIILRRLFMSRINRPPISLSRLARHM 202
+K +RT KS DV TN+KFN +IL+RLFMS++N+ P+SLSRL M
Sbjct: 12 KKTKRTAPKSDDVYLKLTVKLYRFLVRRTNSKFNGVILKRLFMSKVNKAPLSLSRLVEFM 71
Query: 203 KKPTREGLIAVVVGTVSNDVRLYTVPKMTVAALHVTEKARARILAAG 343
+E IAV+VGT+++D+R++ +P M V AL TE+ARARI AG
Sbjct: 72 --TGKEDKIAVLVGTITDDLRVHEIPAMKVTALRFTERARARIEKAG 116
Score = 72.1 bits (169), Expect = 2e-13
Identities = 31/42 (73%), Positives = 37/42 (88%)
Frame = +3
Query: 345 GEILTFDQLALRAPTGRKTVLVQGRRNAREAVRHFGPAPGAP 470
GE LTFDQLALRAP G+ TVL++G +N+REAV+HFGPAPG P
Sbjct: 117 GECLTFDQLALRAPLGQNTVLLRGPKNSREAVKHFGPAPGVP 158
>At5g27850.1 68418.m03341 60S ribosomal protein L18 (RPL18C) 60S
ribosomal protein L18, Arabidopsis thaliana,
SWISSPROT:RL18_ARATH
Length = 187
Score = 91.9 bits (218), Expect = 2e-19
Identities = 47/107 (43%), Positives = 68/107 (63%)
Frame = +2
Query: 23 RKVRRTEVKSQDVXXXXXXXXXXXXXXXTNAKFNQIILRRLFMSRINRPPISLSRLARHM 202
+K +RT KS DV +N+ FN +IL+RLFMS++N+ P+SLSRL M
Sbjct: 12 KKTKRTAPKSDDVYLKLLVKLYRFLVRRSNSNFNAVILKRLFMSKVNKAPLSLSRLVEFM 71
Query: 203 KKPTREGLIAVVVGTVSNDVRLYTVPKMTVAALHVTEKARARILAAG 343
++ IAV+VGT+++D+R++ +P M V AL TE+ARARI AG
Sbjct: 72 --TGKDDKIAVLVGTITDDLRVHEIPAMKVTALRFTERARARIEKAG 116
Score = 72.1 bits (169), Expect = 2e-13
Identities = 31/42 (73%), Positives = 37/42 (88%)
Frame = +3
Query: 345 GEILTFDQLALRAPTGRKTVLVQGRRNAREAVRHFGPAPGAP 470
GE LTFDQLALRAP G+ TVL++G +N+REAV+HFGPAPG P
Sbjct: 117 GECLTFDQLALRAPLGQNTVLLRGPKNSREAVKHFGPAPGVP 158
>At2g47570.1 68415.m05936 60S ribosomal protein L18 (RPL18A)
Length = 135
Score = 75.8 bits (178), Expect = 1e-14
Identities = 40/82 (48%), Positives = 55/82 (67%)
Frame = +2
Query: 149 MSRINRPPISLSRLARHMKKPTREGLIAVVVGTVSNDVRLYTVPKMTVAALHVTEKARAR 328
MS++N+ P+SLSRL R+M ++G IAV+VGTV++DVR+ VP +TV AL TE ARAR
Sbjct: 1 MSKVNKAPLSLSRLVRYMDG--KDGKIAVIVGTVTDDVRIEDVPALTVTALRFTESARAR 58
Query: 329 ILAAGR*DSNV*PVSPPCAHWT 394
I AG ++ PC W+
Sbjct: 59 IHKAGGECLTFDQLALPCPTWS 80
Score = 62.5 bits (145), Expect = 1e-10
Identities = 29/43 (67%), Positives = 33/43 (76%), Gaps = 1/43 (2%)
Frame = +3
Query: 345 GEILTFDQLALRAPT-GRKTVLVQGRRNAREAVRHFGPAPGAP 470
GE LTFDQLAL PT TVL++G +N REAV+HFGPAPG P
Sbjct: 64 GECLTFDQLALPCPTWSENTVLLRGPKNTREAVKHFGPAPGVP 106
>At1g61900.2 68414.m06984 expressed protein contains similarity to
glutamic acid/alanine-rich protein GI:6707830 from
[Trypanosoma congolense]
Length = 413
Score = 29.1 bits (62), Expect = 1.6
Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 5/42 (11%)
Frame = -1
Query: 262 HIIGDCANDYSDQTLACRLL-----HVACQTGQRNRRSVDTA 152
HI G+C+N+ S+QT CR + H+ QT N +++D A
Sbjct: 271 HIGGNCSNELSNQTGCCRAMESYVSHLQKQTLITNLQALDCA 312
>At1g61900.1 68414.m06983 expressed protein contains similarity to
glutamic acid/alanine-rich protein GI:6707830 from
[Trypanosoma congolense]
Length = 433
Score = 29.1 bits (62), Expect = 1.6
Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 5/42 (11%)
Frame = -1
Query: 262 HIIGDCANDYSDQTLACRLL-----HVACQTGQRNRRSVDTA 152
HI G+C+N+ S+QT CR + H+ QT N +++D A
Sbjct: 271 HIGGNCSNELSNQTGCCRAMESYVSHLQKQTLITNLQALDCA 312
>At2g18850.1 68415.m02196 SET domain-containing protein low
similarity to ribulose-1,5-bisphosphate
carboxylase/oxygenase small subunit N-methyltransferase
I [Spinacia oleracea] GI:3403236; contains Pfam profile
PF00856: SET domain
Length = 448
Score = 28.3 bits (60), Expect = 2.8
Identities = 10/17 (58%), Positives = 14/17 (82%)
Frame = -2
Query: 426 HYGDPEPVLSYVQWAHG 376
HYG P P+L+Y++ AHG
Sbjct: 346 HYGLPTPLLNYLRKAHG 362
>At5g11350.1 68418.m01325 endonuclease/exonuclease/phosphatase
family protein contains Pfam profile PF03372:
Endonuclease/Exonuclease/phosphatase family
Length = 754
Score = 27.9 bits (59), Expect = 3.7
Identities = 17/50 (34%), Positives = 24/50 (48%)
Frame = -1
Query: 259 IIGDCANDYSDQTLACRLLHVACQTGQRNRRSVDTAHKQSP*DDLVELGI 110
+ G A SDQ L C + CQ G NR+ D+ + + +DL L I
Sbjct: 468 LAGHEATSSSDQVLPCENMASDCQFGIENRKPDDSGNLSTA-EDLSSLTI 516
>At3g28360.1 68416.m03544 ABC transporter family protein similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1158
Score = 27.9 bits (59), Expect = 3.7
Identities = 12/31 (38%), Positives = 17/31 (54%)
Frame = +3
Query: 348 EILTFDQLALRAPTGRKTVLVQGRRNAREAV 440
E L FD L L+ P+G+ LV G + + V
Sbjct: 290 ETLIFDDLCLKIPSGKTVALVGGSGSGKSTV 320
>At3g59820.1 68416.m06675 calcium-binding mitochondrial
protein-related contains weak similarity to
Calcium-binding mitochondrial protein Anon-60Da
(Swiss-Prot:P91927) [Drosophila melanogaster]
Length = 755
Score = 27.1 bits (57), Expect = 6.4
Identities = 11/36 (30%), Positives = 18/36 (50%)
Frame = +2
Query: 122 NQIILRRLFMSRINRPPISLSRLARHMKKPTREGLI 229
+Q + + S INRPP+ + H R+GL+
Sbjct: 34 SQTVHSHAYHSGINRPPVETKPVTEHKSFTRRDGLL 69
>At3g28380.1 68416.m03546 P-glycoprotein, putative similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1240
Score = 27.1 bits (57), Expect = 6.4
Identities = 13/40 (32%), Positives = 19/40 (47%)
Frame = +3
Query: 321 VPASWLLEGEILTFDQLALRAPTGRKTVLVQGRRNAREAV 440
V ++L E FD L L+ P G+ LV G + + V
Sbjct: 364 VKFTYLSRPETTIFDDLCLKIPAGKTVALVGGSGSGKSTV 403
>At3g28415.1 68416.m03551 P-glycoprotein, putative contains
ATP-binding cassette; related to multi drug resistance
proteins
Length = 1221
Score = 26.6 bits (56), Expect = 8.5
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +3
Query: 360 FDQLALRAPTGRKTVLVQGRRNAREAV 440
FD L LR P+G+ LV G + + V
Sbjct: 356 FDDLCLRIPSGKSVALVGGSGSGKSTV 382
>At3g28390.1 68416.m03547 P-glycoprotein, putative similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1225
Score = 26.6 bits (56), Expect = 8.5
Identities = 11/27 (40%), Positives = 15/27 (55%)
Frame = +3
Query: 360 FDQLALRAPTGRKTVLVQGRRNAREAV 440
FD L LR P+G+ LV G + + V
Sbjct: 365 FDDLCLRVPSGKTVALVGGSGSGKSTV 391
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,013,684
Number of Sequences: 28952
Number of extensions: 194329
Number of successful extensions: 469
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 454
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 465
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 801831960
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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