Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_J17
(388 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C) 38 0.003
At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) simi... 37 0.004
At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A) 33 0.067
At5g66520.1 68418.m08387 pentatricopeptide (PPR) repeat-containi... 29 1.4
At5g21222.1 68418.m02532 protein kinase family protein contains ... 28 1.9
At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase, puta... 28 2.5
At2g21730.1 68415.m02585 mannitol dehydrogenase, putative simila... 28 2.5
At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding ... 27 3.3
At1g34140.1 68414.m04235 polyadenylate-binding protein, putative... 27 3.3
At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase / lon... 27 4.4
At1g79340.1 68414.m09246 latex-abundant protein, putative (AMC7)... 27 4.4
At1g19570.1 68414.m02437 dehydroascorbate reductase, putative si... 27 4.4
At1g19550.1 68414.m02435 dehydroascorbate reductase, putative si... 27 4.4
At5g51660.1 68418.m06405 cleavage and polyadenylation specificit... 27 5.8
At5g21326.1 68418.m02534 protein kinase family protein / NAF dom... 27 5.8
At5g02830.1 68418.m00225 pentatricopeptide (PPR) repeat-containi... 27 5.8
At1g18260.1 68414.m02277 suppressor of lin-12-like protein-relat... 27 5.8
At5g24690.1 68418.m02918 expressed protein 26 7.6
At3g53480.1 68416.m05904 ABC transporter family protein PDR5-lik... 26 7.6
>At4g15000.1 68417.m02304 60S ribosomal protein L27 (RPL27C)
Length = 135
Score = 37.5 bits (83), Expect = 0.003
Identities = 28/72 (38%), Positives = 35/72 (48%), Gaps = 5/72 (6%)
Frame = +2
Query: 77 VIGKDSLMKADVKMKEICIMKLLNYV-LQPTVYE---DIKEVAREYMLEENTDKYSK-SD 241
VI KDS K K + C +KL+NY L PT Y D+KEVA L+ K + +
Sbjct: 53 VIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALQSKDKKVAALKE 112
Query: 242 VVTKFMETFKMG 277
K E FK G
Sbjct: 113 AKAKLEERFKTG 124
>At3g22230.1 68416.m02804 60S ribosomal protein L27 (RPL27B) similar
to 60S RIBOSOMAL PROTEIN L27 GB:P41101 from [Solanum
tuberosum]
Length = 135
Score = 37.1 bits (82), Expect = 0.004
Identities = 28/72 (38%), Positives = 35/72 (48%), Gaps = 5/72 (6%)
Frame = +2
Query: 77 VIGKDSLMKADVKMKEICIMKLLNYV-LQPTVYE---DIKEVAREYMLEENTDKYSK-SD 241
VI KDS K K + C +KL+NY L PT Y D+KEVA L+ K + +
Sbjct: 53 VIRKDSAKKTAKKSRVKCFIKLVNYQHLMPTRYTLDVDLKEVATLDALKSKDKKVTALKE 112
Query: 242 VVTKFMETFKMG 277
K E FK G
Sbjct: 113 AKAKLEERFKTG 124
>At2g32220.1 68415.m03937 60S ribosomal protein L27 (RPL27A)
Length = 135
Score = 33.1 bits (72), Expect = 0.067
Identities = 24/72 (33%), Positives = 32/72 (44%), Gaps = 5/72 (6%)
Frame = +2
Query: 77 VIGKDSLMKADVKMKEICIMKLLNYV-LQPTVYE---DIKEVAREYMLEENTDKYSK-SD 241
VI KDS K K + C K++NY + PT Y D+K V + K + +
Sbjct: 53 VIRKDSAKKTAKKSRVKCFFKVINYQHVMPTRYTLDLDLKNVVSADAISSKDKKVTALKE 112
Query: 242 VVTKFMETFKMG 277
KF E FK G
Sbjct: 113 AKAKFEERFKTG 124
>At5g66520.1 68418.m08387 pentatricopeptide (PPR) repeat-containing
protein contains INTERPRO:IPR002885 PPR repeats
Length = 620
Score = 28.7 bits (61), Expect = 1.4
Identities = 12/42 (28%), Positives = 23/42 (54%)
Frame = +2
Query: 239 DVVTKFMETFKMGMLPRGEVFVHTNAVANGTGPVKVSRILYF 364
+ ++KFME KMG+ P F + TG V+ +++++
Sbjct: 331 EAISKFMEMQKMGIKPNVITFTAVLTACSYTGLVEEGKLIFY 372
>At5g21222.1 68418.m02532 protein kinase family protein contains
Pfam profile: PF00069 protein kinase domain
Length = 831
Score = 28.3 bits (60), Expect = 1.9
Identities = 16/50 (32%), Positives = 30/50 (60%), Gaps = 3/50 (6%)
Frame = +2
Query: 74 VVIGKDSLMK---ADVKMKEICIMKLLNYVLQPTVYEDIKEVAREYMLEE 214
+++ KD ++K A+ +EI IMKL+N+ +YE + A+ Y++ E
Sbjct: 42 MILDKDKVLKHKMAEQIKREISIMKLINHPNVVQLYEVLASKAKIYIVLE 91
>At4g11030.1 68417.m01794 long-chain-fatty-acid--CoA ligase,
putative / long-chain acyl-CoA synthetase, putative
similar to acyl-CoA synthetase (MF7P) gi:1617270 from
Brassica napus
Length = 666
Score = 27.9 bits (59), Expect = 2.5
Identities = 18/58 (31%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
Frame = -2
Query: 291 PRGSMPILKVSMNFVTTSDFEYLSVFS-SSIYSLATSLMSSYTVGCST*FKSFMMQIS 121
P GSM I+ N + EY++V + ++YS + S + G S F+SF++ I+
Sbjct: 508 PNGSMKIIDRKKNIFKLAQGEYVAVENLENVYSQVEVIESIWVYGNS--FESFLVAIA 563
>At2g21730.1 68415.m02585 mannitol dehydrogenase, putative similar
to ELI3-2 (SP|Q02972), sinapyl alcohol dehydrogenase
(Populus tremuloides) (gi:14279694); contains Pfam
zinc-binding dehydrogenase domain PF00107
Length = 376
Score = 27.9 bits (59), Expect = 2.5
Identities = 21/88 (23%), Positives = 42/88 (47%), Gaps = 1/88 (1%)
Frame = +2
Query: 68 FDVVIGKDSLMKADVK-MKEICIMKLLNYVLQPTVYEDIKEVAREYMLEENTDKYSKSDV 244
F +V+G+ + + + MKE ++L + + + DI E+ + + D+ +KSDV
Sbjct: 285 FSLVLGRKMVGGSQIGGMKET--QEMLEFCAKHKIVSDI-ELIKMSDINSAMDRLAKSDV 341
Query: 245 VTKFMETFKMGMLPRGEVFVHTNAVANG 328
+F+ +LP + T V +G
Sbjct: 342 RYRFVIDVANSLLPESSAEILTEQVDHG 369
>At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding
bromodomain-containing protein low similarity to
SP|P51123 Transcription initiation factor TFIID 230 kDa
subunit {Drosophila melanogaster}; contains Pfam
profiles: PF00439 bromodomain, PF00240: Ubiquitin family
Length = 1700
Score = 27.5 bits (58), Expect = 3.3
Identities = 12/41 (29%), Positives = 17/41 (41%)
Frame = +3
Query: 162 QLCTRTSRKSRGNICWRKTRTSTRNPMLLRNSWRPSKWACY 284
Q+C+ R + G CW K R + P+ L P Y
Sbjct: 783 QVCSDLERDANGKACWSKKRKFDKIPLGLNTLVAPEDVCSY 823
>At1g34140.1 68414.m04235 polyadenylate-binding protein, putative /
PABP, putative non-consensus splice donor TA at exon 1;
similar to polyadenylate-binding protein
(poly(A)-binding protein) from [Triticum aestivum]
GI:1737492, [Nicotiana tabacum] GI:7673355, {Arabidopsis
thaliana} SP|P42731; contains InterPro entry IPR000504:
RNA-binding region RNP-1 (RNA recognition motif) (RRM)
Length = 407
Score = 27.5 bits (58), Expect = 3.3
Identities = 18/63 (28%), Positives = 34/63 (53%), Gaps = 5/63 (7%)
Frame = +2
Query: 176 DIKEVAREYMLEENTDKYSKS--DVV--TKFMETF-KMGMLPRGEVFVHTNAVANGTGPV 340
+++++ R+ + + Y K+ D V TK E F + G + +V VH+N ++ G G V
Sbjct: 209 ELEKIIRDMKTRKGMNLYVKNLDDSVDNTKLEELFSEFGTITSCKVMVHSNGISKGVGFV 268
Query: 341 KVS 349
+ S
Sbjct: 269 EFS 271
>At4g23850.1 68417.m03429 long-chain-fatty-acid--CoA ligase /
long-chain acyl-CoA synthetase nearly identical to
acyl-CoA synthetase (MF7P) from Brassica napus
[gi:1617270]
Length = 666
Score = 27.1 bits (57), Expect = 4.4
Identities = 19/58 (32%), Positives = 31/58 (53%), Gaps = 1/58 (1%)
Frame = -2
Query: 291 PRGSMPILKVSMNFVTTSDFEYLSVFS-SSIYSLATSLMSSYTVGCST*FKSFMMQIS 121
P GSM I+ N S EY++V + +IY ++ S + G S F+SF++ I+
Sbjct: 508 PDGSMKIIDRKKNIFKLSQGEYVAVENIENIYGEVQAVDSVWVYGNS--FESFLIAIA 563
>At1g79340.1 68414.m09246 latex-abundant protein, putative (AMC7) /
caspase family protein similar to latex-abundant protein
[Hevea brasiliensis] gb:AAD13216; contains Pfam domain,
PF00656: ICE-like protease (caspase) p20 domain
Length = 418
Score = 27.1 bits (57), Expect = 4.4
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = +1
Query: 178 HQGSREGIYAGGKHGQV 228
H GS+E +YAGG G V
Sbjct: 316 HVGSKEEVYAGGSRGSV 332
>At1g19570.1 68414.m02437 dehydroascorbate reductase, putative
similar to GB:BAA90672 from (Oryza sativa)
Length = 213
Score = 27.1 bits (57), Expect = 4.4
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -2
Query: 294 SPRGSMPILKVSMNFVTTSD 235
SP+G +P+LK+ +VT SD
Sbjct: 55 SPQGKVPVLKIDDKWVTDSD 74
>At1g19550.1 68414.m02435 dehydroascorbate reductase, putative
similar to dehydroascorbate reductase [Arabidopsis
thaliana] gi|10952514|gb|AAG24946
Length = 153
Score = 27.1 bits (57), Expect = 4.4
Identities = 10/20 (50%), Positives = 15/20 (75%)
Frame = -2
Query: 294 SPRGSMPILKVSMNFVTTSD 235
SP+G +P+LK+ +VT SD
Sbjct: 19 SPQGKVPVLKIDDKWVTDSD 38
>At5g51660.1 68418.m06405 cleavage and polyadenylation specificity
factor (CPSF) A subunit C-terminal domain-containing
protein similar to SP|Q9EPU4 Cleavage and
polyadenylation specificity factor, 160 kDa subunit
(CPSF 160 kDa subunit) {Mus musculus}; contains Pfam
profile PF03178: CPSF A subunit region
Length = 1442
Score = 26.6 bits (56), Expect = 5.8
Identities = 19/56 (33%), Positives = 29/56 (51%)
Frame = +1
Query: 106 RCENEGDLHHETLELRTAANCVRGHQGSREGIYAGGKHGQVLEIRCCYEIHGDLQN 273
R + EG+ T ELR RG G +G+Y G LE+ C Y +HG++++
Sbjct: 74 RAQEEGN----TQELRNPKLAKRG--GVMDGVY-----GVSLELVCHYRLHGNVES 118
>At5g21326.1 68418.m02534 protein kinase family protein / NAF
domain-containing protein contains Pfam profiles:
PF00069 protein kinase domain, PF03822 NAF domain
Length = 439
Score = 26.6 bits (56), Expect = 5.8
Identities = 14/49 (28%), Positives = 28/49 (57%), Gaps = 3/49 (6%)
Frame = +2
Query: 77 VIGKDSLMK---ADVKMKEICIMKLLNYVLQPTVYEDIKEVAREYMLEE 214
++ K+ ++K A+ +EIC MKL+N+ +YE + + Y++ E
Sbjct: 43 ILDKEKVLKHKMAEQIRREICTMKLINHPNVVRLYEVLASKTKIYIVLE 91
>At5g02830.1 68418.m00225 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 852
Score = 26.6 bits (56), Expect = 5.8
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +1
Query: 205 AGGKHGQVLEIRCCYEIHGDLQN 273
A K+G +LE+R C I+ D++N
Sbjct: 638 ARSKYGSLLEVRQCLAIYQDMRN 660
>At1g18260.1 68414.m02277 suppressor of lin-12-like protein-related
/ sel-1 protein-related similar to Sel-1 homolog
precursor (Suppressor of lin-12-like protein)
(Sel-1L)(SP:Q9UBV2) {Homo sapiens}
Length = 678
Score = 26.6 bits (56), Expect = 5.8
Identities = 18/52 (34%), Positives = 27/52 (51%), Gaps = 2/52 (3%)
Frame = -1
Query: 250 RNNIGFRVLVRVF--LQHIFPRDFLDVLVHSWLQYVVQEFHDADLLHFHICL 101
R N VLVRV L ++P+ V +W++ VV E +A +L +CL
Sbjct: 591 RRNYADTVLVRVVDSLPEVYPK------VETWIENVVFEEGNATILTLFVCL 636
>At5g24690.1 68418.m02918 expressed protein
Length = 521
Score = 26.2 bits (55), Expect = 7.6
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = -1
Query: 220 RVFLQHIFPRDFLDVLVHSW 161
R+FL IF R F+D +++ W
Sbjct: 165 RMFLAEIFDRKFVDAVLNEW 184
>At3g53480.1 68416.m05904 ABC transporter family protein PDR5-like
ABC transporter, Spirodela polyrrhiza, EMBL:Z70524
Length = 1450
Score = 26.2 bits (55), Expect = 7.6
Identities = 14/43 (32%), Positives = 22/43 (51%)
Frame = -2
Query: 225 LSVFSSSIYSLATSLMSSYTVGCST*FKSFMMQISFIFTSAFM 97
LS F S +++ + + YT S FK F++ + FTS M
Sbjct: 632 LSFFESLVWTCLSYYVIGYTPEASRFFKQFILLFAVHFTSISM 674
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,096,621
Number of Sequences: 28952
Number of extensions: 167534
Number of successful extensions: 559
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 546
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 559
length of database: 12,070,560
effective HSP length: 73
effective length of database: 9,957,064
effective search space used: 547638520
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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