BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_J10
(484 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g04040.1 68416.m00426 expressed protein 34 0.058
At5g18250.1 68418.m02145 expressed protein similar to unknown pr... 29 1.6
At5g25850.1 68418.m03067 F-box family protein contains F-box dom... 27 5.0
At3g50910.1 68416.m05574 expressed protein 27 5.0
At5g63720.1 68418.m07998 hypothetical protein 27 6.6
At5g58520.1 68418.m07328 protein kinase family protein contains ... 27 6.6
At3g10360.1 68416.m01242 pumilio/Puf RNA-binding domain-containi... 27 8.8
At3g03060.1 68416.m00302 AAA-type ATPase family protein contains... 27 8.8
At1g15280.2 68414.m01829 glycine-rich protein 27 8.8
At1g15280.1 68414.m01828 glycine-rich protein 27 8.8
>At3g04040.1 68416.m00426 expressed protein
Length = 134
Score = 33.9 bits (74), Expect = 0.058
Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 1/46 (2%)
Frame = -3
Query: 326 GRELSPCCNHCAGGVVDDVE-HTLVVCPAWRESFRDLASDVGRVLS 192
G+E+ C+HCAG + ++E + V P R+SF + S VG S
Sbjct: 5 GKEIGSRCHHCAGPLTKNLETNEWTVAPFIRDSFSMIGSAVGGTAS 50
>At5g18250.1 68418.m02145 expressed protein similar to unknown
protein (dbj|BAA90342.1)
Length = 133
Score = 29.1 bits (62), Expect = 1.6
Identities = 16/46 (34%), Positives = 25/46 (54%), Gaps = 1/46 (2%)
Frame = -3
Query: 326 GRELSPCCNHCAGGVVDDVEHT-LVVCPAWRESFRDLASDVGRVLS 192
G ++ C+HCAG + ++E + V P R+SF + S VG S
Sbjct: 5 GHDIEIRCHHCAGPLTRNLETSKWTVDPFIRDSFSMIGSAVGGTAS 50
>At5g25850.1 68418.m03067 F-box family protein contains F-box domain
Pfam:PF00646
Length = 481
Score = 27.5 bits (58), Expect = 5.0
Identities = 16/38 (42%), Positives = 20/38 (52%)
Frame = +1
Query: 370 IDMRVYFLVNYSYISLFALLYNKYGSINHNCMCLHKVR 483
+++ YFL N S + L NKYGS N LHK R
Sbjct: 432 LNLAKYFLENSSILQKLTLHPNKYGSTYAN--MLHKFR 467
>At3g50910.1 68416.m05574 expressed protein
Length = 447
Score = 27.5 bits (58), Expect = 5.0
Identities = 19/52 (36%), Positives = 26/52 (50%), Gaps = 6/52 (11%)
Frame = +1
Query: 202 LPTSDAKSLKDSLHAGHTTNVCSTSSTT------PPAQ*LQHGESSLPSVPT 339
+PT A +L L G +T+V S STT PP L+ G+ LP+ T
Sbjct: 1 MPTFSAIALDRMLEPGASTSVESVPSTTNLFYSKPPISKLEKGKGKLPNERT 52
>At5g63720.1 68418.m07998 hypothetical protein
Length = 492
Score = 27.1 bits (57), Expect = 6.6
Identities = 14/35 (40%), Positives = 17/35 (48%)
Frame = +1
Query: 229 KDSLHAGHTTNVCSTSSTTPPAQ*LQHGESSLPSV 333
K LH +NV +S TTPPA Q S + V
Sbjct: 71 KTVLHGAKPSNVADSSETTPPAMLSQINGSHITRV 105
>At5g58520.1 68418.m07328 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 604
Score = 27.1 bits (57), Expect = 6.6
Identities = 8/34 (23%), Positives = 18/34 (52%)
Frame = -1
Query: 319 NSLRVVITVPAESSTTSSTRWSCVPRGGSPSGTW 218
+++++ + +P+ S S W + GG+ G W
Sbjct: 308 STIQIQVPLPSSSFAVSVDEWQTIQSGGNEIGKW 341
>At3g10360.1 68416.m01242 pumilio/Puf RNA-binding domain-containing
protein similar to RNA binding protein PufA GB:AAD39751
[Dictyostelium discoideum] and similar to Pumilio
protein GB:A46221 [Drosophila sp.]
Length = 1003
Score = 26.6 bits (56), Expect = 8.8
Identities = 16/48 (33%), Positives = 19/48 (39%), Gaps = 2/48 (4%)
Frame = +3
Query: 114 HDGFATGRDNRPSPRAAD--HRPHERRQGEDPTHVGRQVPEGLPPRGT 251
HD + N P+ P R + D VGR GLPP GT
Sbjct: 277 HDSLHSTAKNSPNTMLGSTMSSPVPRNRTPDSHLVGRSTASGLPPIGT 324
>At3g03060.1 68416.m00302 AAA-type ATPase family protein contains a
ATP/GTP-binding site motif A (P-loop), PROSITE:PS00017
Length = 639
Score = 26.6 bits (56), Expect = 8.8
Identities = 16/54 (29%), Positives = 25/54 (46%)
Frame = -3
Query: 239 RESFRDLASDVGRVLSLPSLVRAMVGSSRAWAVVSSCCEAIMLAKEDAEQERER 78
RE + + S V R+L PSL+R W+ S + + KE A + +R
Sbjct: 313 REGAKVIWSYVDRILGQPSLIRESSRGKYPWSGSLSRVMSTLRGKESASKNGKR 366
>At1g15280.2 68414.m01829 glycine-rich protein
Length = 585
Score = 26.6 bits (56), Expect = 8.8
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +1
Query: 196 RTLPTSDAKSLKDSLHAGHTTNVCSTSSTTPP 291
R PT+ AK+ HA V S SS +PP
Sbjct: 255 RRSPTAPAKTENQGAHAKKNVAVSSLSSASPP 286
>At1g15280.1 68414.m01828 glycine-rich protein
Length = 584
Score = 26.6 bits (56), Expect = 8.8
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = +1
Query: 196 RTLPTSDAKSLKDSLHAGHTTNVCSTSSTTPP 291
R PT+ AK+ HA V S SS +PP
Sbjct: 254 RRSPTAPAKTENQGAHAKKNVAVSSLSSASPP 285
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,894,256
Number of Sequences: 28952
Number of extensions: 142876
Number of successful extensions: 485
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 480
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 485
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 829097472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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