BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_J07 (654 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC32C12.02 |ste11|aff1, stex|transcription factor Ste11|Schizo... 29 0.77 SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pomb... 27 1.8 SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr... 27 1.8 SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce... 26 5.5 SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual 25 7.2 SPBC1105.18c ||SPBC887.21c|peptide release factor|Schizosaccharo... 25 9.5 SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharo... 25 9.5 SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc... 25 9.5 >SPBC32C12.02 |ste11|aff1, stex|transcription factor Ste11|Schizosaccharomyces pombe|chr 2|||Manual Length = 468 Score = 28.7 bits (61), Expect = 0.77 Identities = 13/24 (54%), Positives = 16/24 (66%) Frame = +3 Query: 534 SGYNGYSGQSSTNSGHTYDSSGRS 605 SGY S S TNSGHT +++ RS Sbjct: 372 SGYTCVSSSSVTNSGHTENNTWRS 395 >SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 224 Score = 27.5 bits (58), Expect = 1.8 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = +3 Query: 543 NGYSGQSSTNSGHTYDSSGRSQGYTGQVENTQELDST 653 N +GQ+ST++GH SS Y N++ + S+ Sbjct: 25 NSSNGQTSTSAGHPSSSSSTLPNYAASSLNSRPVSSS 61 >SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 475 Score = 27.5 bits (58), Expect = 1.8 Identities = 12/26 (46%), Positives = 14/26 (53%) Frame = +3 Query: 495 TYSASDSQSSRYHSGYNGYSGQSSTN 572 +Y SDSQ S +H G N GQ N Sbjct: 267 SYKKSDSQQSFHHKGRNTRKGQRHNN 292 Score = 26.2 bits (55), Expect = 4.1 Identities = 11/47 (23%), Positives = 22/47 (46%) Frame = +3 Query: 501 SASDSQSSRYHSGYNGYSGQSSTNSGHTYDSSGRSQGYTGQVENTQE 641 S S + +HS +G++ + H+Y S Q + + NT++ Sbjct: 240 SEDSSSQAPHHSSSSGHAPSQQGGNKHSYKKSDSQQSFHHKGRNTRK 286 >SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces pombe|chr 1|||Manual Length = 2310 Score = 25.8 bits (54), Expect = 5.5 Identities = 15/40 (37%), Positives = 22/40 (55%) Frame = -3 Query: 259 PFGVALNHPYCARVQNSLPYFPSEHHKSQIYFLHPRNFWN 140 P+ +AL A +Q + +E KS ++ L PRNFWN Sbjct: 799 PYILALKESADALMQFGSYEYATELLKSCLFLL-PRNFWN 837 >SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual Length = 583 Score = 25.4 bits (53), Expect = 7.2 Identities = 12/35 (34%), Positives = 17/35 (48%) Frame = +3 Query: 507 SDSQSSRYHSGYNGYSGQSSTNSGHTYDSSGRSQG 611 +D +S+ Y GY+ GQS T + G S G Sbjct: 191 ADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSG 225 >SPBC1105.18c ||SPBC887.21c|peptide release factor|Schizosaccharomyces pombe|chr 2|||Manual Length = 162 Score = 25.0 bits (52), Expect = 9.5 Identities = 9/26 (34%), Positives = 15/26 (57%) Frame = +1 Query: 61 LTAFSCDISFSCGKTWNCTREKEDFQ 138 L AFSC + TW C++++ +Q Sbjct: 18 LNAFSCLAELNFRHTWYCSKKETPYQ 43 >SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharomyces pombe|chr 2|||Manual Length = 399 Score = 25.0 bits (52), Expect = 9.5 Identities = 11/28 (39%), Positives = 16/28 (57%) Frame = -1 Query: 237 IHIVRAFKIPSHISPLSTIKARFTSFIR 154 ++I+R FK+ S S LS IK +R Sbjct: 267 LNIIREFKLESDYSSLSAIKGHLFKLMR 294 >SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces pombe|chr 1|||Manual Length = 503 Score = 25.0 bits (52), Expect = 9.5 Identities = 10/30 (33%), Positives = 15/30 (50%) Frame = +3 Query: 537 GYNGYSGQSSTNSGHTYDSSGRSQGYTGQV 626 G GY G T+ Y+ +GR +G G + Sbjct: 297 GRRGYPGYMYTDLSTIYERAGRVEGRNGSI 326 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,504,480 Number of Sequences: 5004 Number of extensions: 46047 Number of successful extensions: 123 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 119 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 123 length of database: 2,362,478 effective HSP length: 70 effective length of database: 2,012,198 effective search space used: 295793106 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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