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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_J07
         (654 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC32C12.02 |ste11|aff1, stex|transcription factor Ste11|Schizo...    29   0.77 
SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces pomb...    27   1.8  
SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr...    27   1.8  
SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyce...    26   5.5  
SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual        25   7.2  
SPBC1105.18c ||SPBC887.21c|peptide release factor|Schizosaccharo...    25   9.5  
SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1 |Schizosaccharo...    25   9.5  
SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyc...    25   9.5  

>SPBC32C12.02 |ste11|aff1, stex|transcription factor
           Ste11|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 468

 Score = 28.7 bits (61), Expect = 0.77
 Identities = 13/24 (54%), Positives = 16/24 (66%)
 Frame = +3

Query: 534 SGYNGYSGQSSTNSGHTYDSSGRS 605
           SGY   S  S TNSGHT +++ RS
Sbjct: 372 SGYTCVSSSSVTNSGHTENNTWRS 395


>SPAC17A5.10 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 224

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 12/37 (32%), Positives = 20/37 (54%)
 Frame = +3

Query: 543 NGYSGQSSTNSGHTYDSSGRSQGYTGQVENTQELDST 653
           N  +GQ+ST++GH   SS     Y     N++ + S+
Sbjct: 25  NSSNGQTSTSAGHPSSSSSTLPNYAASSLNSRPVSSS 61


>SPAC1527.03 |||RNA-binding protein|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 475

 Score = 27.5 bits (58), Expect = 1.8
 Identities = 12/26 (46%), Positives = 14/26 (53%)
 Frame = +3

Query: 495 TYSASDSQSSRYHSGYNGYSGQSSTN 572
           +Y  SDSQ S +H G N   GQ   N
Sbjct: 267 SYKKSDSQQSFHHKGRNTRKGQRHNN 292



 Score = 26.2 bits (55), Expect = 4.1
 Identities = 11/47 (23%), Positives = 22/47 (46%)
 Frame = +3

Query: 501 SASDSQSSRYHSGYNGYSGQSSTNSGHTYDSSGRSQGYTGQVENTQE 641
           S   S  + +HS  +G++      + H+Y  S   Q +  +  NT++
Sbjct: 240 SEDSSSQAPHHSSSSGHAPSQQGGNKHSYKKSDSQQSFHHKGRNTRK 286


>SPAC27E2.09 |mak2|phk1|histidine kinase Mak2 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 2310

 Score = 25.8 bits (54), Expect = 5.5
 Identities = 15/40 (37%), Positives = 22/40 (55%)
 Frame = -3

Query: 259 PFGVALNHPYCARVQNSLPYFPSEHHKSQIYFLHPRNFWN 140
           P+ +AL     A +Q     + +E  KS ++ L PRNFWN
Sbjct: 799 PYILALKESADALMQFGSYEYATELLKSCLFLL-PRNFWN 837


>SPAC869.01 |||amidase |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 583

 Score = 25.4 bits (53), Expect = 7.2
 Identities = 12/35 (34%), Positives = 17/35 (48%)
 Frame = +3

Query: 507 SDSQSSRYHSGYNGYSGQSSTNSGHTYDSSGRSQG 611
           +D +S+ Y  GY+   GQS      T +  G S G
Sbjct: 191 ADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSG 225


>SPBC1105.18c ||SPBC887.21c|peptide release
           factor|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 162

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 9/26 (34%), Positives = 15/26 (57%)
 Frame = +1

Query: 61  LTAFSCDISFSCGKTWNCTREKEDFQ 138
           L AFSC    +   TW C++++  +Q
Sbjct: 18  LNAFSCLAELNFRHTWYCSKKETPYQ 43


>SPBC36B7.04 |||tRNA dihydrouridine synthase Dus1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 399

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 11/28 (39%), Positives = 16/28 (57%)
 Frame = -1

Query: 237 IHIVRAFKIPSHISPLSTIKARFTSFIR 154
           ++I+R FK+ S  S LS IK      +R
Sbjct: 267 LNIIREFKLESDYSSLSAIKGHLFKLMR 294


>SPAC637.05c |vma2||V-type ATPase V1 subunit B |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 503

 Score = 25.0 bits (52), Expect = 9.5
 Identities = 10/30 (33%), Positives = 15/30 (50%)
 Frame = +3

Query: 537 GYNGYSGQSSTNSGHTYDSSGRSQGYTGQV 626
           G  GY G   T+    Y+ +GR +G  G +
Sbjct: 297 GRRGYPGYMYTDLSTIYERAGRVEGRNGSI 326


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,504,480
Number of Sequences: 5004
Number of extensions: 46047
Number of successful extensions: 123
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 119
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 123
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 295793106
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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