BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_I23
(342 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g72760.1 68414.m08413 protein kinase family protein contains ... 30 0.35
At3g50120.1 68416.m05479 expressed protein contains Pfam profile... 29 0.81
At1g47450.1 68414.m05260 expressed protein 29 1.1
At3g53390.1 68416.m05892 transducin family protein / WD-40 repea... 28 1.9
At3g06520.1 68416.m00756 agenet domain-containing protein contai... 28 1.9
At2g27570.1 68415.m03340 sulfotransferase family protein similar... 27 3.3
At2g13680.1 68415.m01508 glycosyl transferase family 48 protein ... 27 3.3
At2g32810.1 68415.m04016 beta-galactosidase, putative / lactase,... 27 4.3
At1g05850.1 68414.m00612 chitinase-like protein 1 (CTL1) similar... 27 4.3
At1g31830.2 68414.m03909 amino acid permease family protein weak... 26 7.6
At1g31830.1 68414.m03910 amino acid permease family protein weak... 26 7.6
At4g03490.1 68417.m00476 ankyrin repeat family protein contains ... 25 10.0
At3g45070.1 68416.m04858 sulfotransferase family protein similar... 25 10.0
At2g37160.1 68415.m04559 transducin family protein / WD-40 repea... 25 10.0
>At1g72760.1 68414.m08413 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 697
Score = 30.3 bits (65), Expect = 0.35
Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 4/64 (6%)
Frame = +3
Query: 117 PQCEPGHVKLWDGYSLLYIDGNEKAHNQDLGYAGSCVRK-FSTMPFLFCDL---NDVCNY 284
PQC HV+L D + D ++AH L + G C RK + D+ N + NY
Sbjct: 43 PQCVLIHVQLGDTGGHFHQDNPDEAHEFFLPFRGFCARKGIIAKEVILHDIDISNAIVNY 102
Query: 285 ASRN 296
+ N
Sbjct: 103 ITNN 106
>At3g50120.1 68416.m05479 expressed protein contains Pfam profile
PF03140: Plant protein of unknown function; expression
supported by MPSS
Length = 531
Score = 29.1 bits (62), Expect = 0.81
Identities = 14/30 (46%), Positives = 17/30 (56%), Gaps = 1/30 (3%)
Frame = +2
Query: 5 NRYYWKKWPTWPTRCTMRHYRLLN-WYIIS 91
NRYY KW W R T++H N W I+S
Sbjct: 477 NRYYDHKWNAW--RATLKHKYFNNPWAIVS 504
>At1g47450.1 68414.m05260 expressed protein
Length = 169
Score = 28.7 bits (61), Expect = 1.1
Identities = 15/53 (28%), Positives = 30/53 (56%), Gaps = 4/53 (7%)
Frame = +3
Query: 87 LVRHSQREVVPQCEPGHVKLWDGYSLLYIDGNEKAHNQDLGYA----GSCVRK 233
++ RE++ + H +L +G+S +Y+ GN A ++D+ Y+ CVR+
Sbjct: 105 IINECCREILKMGKDCHFRLVEGWSAMYVYGNSIA-SKDIPYSKQSWNECVRR 156
>At3g53390.1 68416.m05892 transducin family protein / WD-40 repeat
family protein contains 5 WD-40 repeats (PF00400);
similar to Dystrophia myotonica-containing WD repeat
motif protein DMR-N9 protein (DMWD) (DM9) (SP:Q08274)
[Mus musculus]; simlar to DMR protein GI:18028289 [Homo
sapiens];
Length = 558
Score = 27.9 bits (59), Expect = 1.9
Identities = 11/29 (37%), Positives = 18/29 (62%)
Frame = +3
Query: 63 TDYLTGILLVRHSQREVVPQCEPGHVKLW 149
T+ L+G++ +Q VV C GH+K+W
Sbjct: 487 TEPLSGLMF---TQESVVTACREGHIKIW 512
>At3g06520.1 68416.m00756 agenet domain-containing protein contains
Pfam PF05641: Agenet domain
Length = 466
Score = 27.9 bits (59), Expect = 1.9
Identities = 11/26 (42%), Positives = 14/26 (53%)
Frame = -3
Query: 265 RSQKRNGIVLNFRTHEPAYPRSWLCA 188
R Q G ++ R+ E AY SW CA
Sbjct: 166 RDQYEKGALVEVRSEEKAYKGSWYCA 191
Score = 25.4 bits (53), Expect = 10.0
Identities = 12/33 (36%), Positives = 19/33 (57%), Gaps = 2/33 (6%)
Frame = -3
Query: 280 LQTSLRSQK--RNGIVLNFRTHEPAYPRSWLCA 188
L++ R++K NG+ + R+ EP Y SW A
Sbjct: 323 LKSCERAEKVFNNGMEVEVRSDEPGYEASWFSA 355
>At2g27570.1 68415.m03340 sulfotransferase family protein similar to
steroid sulfotransferase from [Brassica napus]
GI:3420008, GI:3420006; contains Pfam profile PF00685:
Sulfotransferase domain
Length = 273
Score = 27.1 bits (57), Expect = 3.3
Identities = 16/49 (32%), Positives = 25/49 (51%)
Frame = -3
Query: 277 QTSLRSQKRNGIVLNFRTHEPAYPRSWLCAFSFPSIYNSE*PSHNFTCP 131
Q + + Q N IV +F P +WL A +F ++ S+ PSH+ P
Sbjct: 57 QKNFKPQDTNIIVASF----PKCGTTWLKALTFSLVHRSKHPSHDHHHP 101
>At2g13680.1 68415.m01508 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1923
Score = 27.1 bits (57), Expect = 3.3
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +3
Query: 183 EKAHNQDLGYAGSCVRKFSTMPFLFCDLNDVCNYASR 293
EKAH D G VR+F T+ F + ++ + ASR
Sbjct: 71 EKAHRLDPSSGGRGVRQFKTLLFQRLERDNASSLASR 107
>At2g32810.1 68415.m04016 beta-galactosidase, putative / lactase,
putative similar to beta-galactosidase GI:7939617 from
[Lycopersicon esculentum]
Length = 887
Score = 26.6 bits (56), Expect = 4.3
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +3
Query: 57 ATTDYLTGILLVRHSQREVVPQCEPGHV 140
+T DY+ G + + EV CE GHV
Sbjct: 779 STPDYINGTMSINSVAPEVHLHCEDGHV 806
>At1g05850.1 68414.m00612 chitinase-like protein 1 (CTL1) similar to
class I chitinase GI:7798656 from [Halimolobos perplexa
var. perplexa]; contains Pfam profile PF00182: Chitinase
class I; identical to cDNA chitinase-like protein 1
(CTL1) CTL1-ELP1 allele GI:17226328
Length = 321
Score = 26.6 bits (56), Expect = 4.3
Identities = 6/23 (26%), Positives = 16/23 (69%), Gaps = 1/23 (4%)
Frame = -2
Query: 68 ICSGAW-CTWWARWAISSSNTDF 3
+C+ W C+WW+++ + + +D+
Sbjct: 41 VCTQGWECSWWSKYCCNQTISDY 63
>At1g31830.2 68414.m03909 amino acid permease family protein weak
similarity to y+LAT1a (amino acid transporter) [Mus
musculus] GI:3970791; contains Pfam profile PF00324:
Amino acid permease
Length = 479
Score = 25.8 bits (54), Expect = 7.6
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2
Query: 59 GAWCTWWARWAISSSN 12
GAW WW + A ++SN
Sbjct: 290 GAWLRWWVQAAAATSN 305
>At1g31830.1 68414.m03910 amino acid permease family protein weak
similarity to y+LAT1a (amino acid transporter) [Mus
musculus] GI:3970791; contains Pfam profile PF00324:
Amino acid permease
Length = 495
Score = 25.8 bits (54), Expect = 7.6
Identities = 8/16 (50%), Positives = 11/16 (68%)
Frame = -2
Query: 59 GAWCTWWARWAISSSN 12
GAW WW + A ++SN
Sbjct: 306 GAWLRWWVQAAAATSN 321
>At4g03490.1 68417.m00476 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 587
Score = 25.4 bits (53), Expect = 10.0
Identities = 11/26 (42%), Positives = 18/26 (69%), Gaps = 4/26 (15%)
Frame = +3
Query: 135 HVKLWDGYSLLY----IDGNEKAHNQ 200
H+KLW+ YSL++ DG +K ++Q
Sbjct: 561 HLKLWNHYSLVFSGHLYDGPKKTYSQ 586
>At3g45070.1 68416.m04858 sulfotransferase family protein similar to
steroid sulfotransferase 3 [Brassica napus] GI:3420008,
steroid sulfotransferase 1 [Brassica napus] GI:3420004;
contains Pfam profile PF00685: Sulfotransferase domain
Length = 323
Score = 25.4 bits (53), Expect = 10.0
Identities = 15/45 (33%), Positives = 24/45 (53%)
Frame = -3
Query: 277 QTSLRSQKRNGIVLNFRTHEPAYPRSWLCAFSFPSIYNSE*PSHN 143
Q S + Q + IV +F P +WL A +F ++ S+ PSH+
Sbjct: 52 QKSFKPQDTDIIVASF----PKCGTTWLKALTFALLHRSKQPSHD 92
>At2g37160.1 68415.m04559 transducin family protein / WD-40 repeat
family protein contains 4 WD-40 repeats (PF00400);
similar to Dystrophia myotonica-containing WD repeat
motif protein DMR-N9 protein (DMWD) (DM9) (SP:Q08274)
[Mus musculus]; simlar to DMR protein GI:18028289 [Homo
sapiens];
Length = 544
Score = 25.4 bits (53), Expect = 10.0
Identities = 9/29 (31%), Positives = 18/29 (62%)
Frame = +3
Query: 63 TDYLTGILLVRHSQREVVPQCEPGHVKLW 149
T+ L+G++ +Q ++ C GH+K+W
Sbjct: 488 TEPLSGLIF---TQESLITACREGHLKIW 513
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,257,235
Number of Sequences: 28952
Number of extensions: 141749
Number of successful extensions: 329
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 323
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 329
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 409426656
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -