Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_I12
(570 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase f... 55 4e-08
At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam ... 33 0.18
At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to polyu... 32 0.23
At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC1... 32 0.31
At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding ... 30 1.2
At4g25350.1 68417.m03648 EXS family protein / ERD1/XPR1/SYG1 fam... 29 1.7
At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 fam... 29 1.7
At2g30100.1 68415.m03663 ubiquitin family protein low similarity... 29 2.2
At3g60860.1 68416.m06808 guanine nucleotide exchange family prot... 29 2.9
At2g03240.1 68415.m00277 EXS family protein / ERD1/XPR1/SYG1 fam... 29 2.9
At1g02990.2 68414.m00269 expressed protein similar to mature-par... 29 2.9
At1g02990.1 68414.m00270 expressed protein similar to mature-par... 29 2.9
At1g25390.1 68414.m03152 protein kinase family protein contains ... 28 3.8
At1g14040.1 68414.m01660 EXS family protein / ERD1/XPR1/SYG1 fam... 28 3.8
At3g20350.1 68416.m02578 expressed protein 28 5.0
At1g69480.1 68414.m07983 EXS family protein / ERD1/XPR1/SYG1 fam... 28 5.0
At5g22070.1 68418.m02570 expressed protein contains Pfam profile... 27 6.7
At4g28650.1 68417.m04095 leucine-rich repeat transmembrane prote... 27 6.7
At3g44530.1 68416.m04786 transducin family protein / WD-40 repea... 27 6.7
At3g16555.1 68416.m02115 F-box family protein contains F-box dom... 27 6.7
At3g18200.1 68416.m02315 nodulin MtN21 family protein similar to... 27 8.8
>At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to synaptic glycoprotein SC2 spliced
variant from Homo sapiens [EMBL:AF038958], SC2 from
Rattus sp. [gi:256994]; contains Pfam
3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
Length = 310
Score = 54.8 bits (126), Expect = 4e-08
Identities = 35/108 (32%), Positives = 56/108 (51%), Gaps = 9/108 (8%)
Frame = +1
Query: 211 MEIEILSVAGSKPL-GKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEA------KG 369
M++ ++S +G + L + L + +T+ +++E H K YP RQ + L K
Sbjct: 1 MKVTVVSRSGREVLKAPLDLPDSATVADLQEAFHKRAK-KFYPSRQRLTLPVTPGSKDKP 59
Query: 370 KTLNDEDTLKSLNIQNGYKLYV--KDLGPQISWKNVFLAEYAGPLFVY 507
LN + +LK N L V KDLG Q+S++ +F EY GPL +Y
Sbjct: 60 VVLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYLGPLLIY 107
>At1g53930.1 68414.m06139 ubiquitin family protein contains Pfam
profile: PF00240 ubiquitin family
Length = 158
Score = 32.7 bits (71), Expect = 0.18
Identities = 26/76 (34%), Positives = 41/76 (53%)
Frame = +1
Query: 208 IMEIEILSVAGSKPLGKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDE 387
+M I I ++ G + + +++ TI+N+K KI K + D+Q +L KGK L D
Sbjct: 85 VMFIFIKTLTGRRIV--LEVENCDTIENIKAKIQD--KQGIPVDQQ--RLIFKGKQLEDG 138
Query: 388 DTLKSLNIQNGYKLYV 435
TL NIQN L++
Sbjct: 139 LTLADYNIQNDSILHL 154
>At3g09790.1 68416.m01163 polyubiquitin (UBQ8) identical to
polyubiquitin (ubq8) GI:870793, GB:L05917 [Arabidopsis
thaliana] (Genetics 139 (2), 921-939 (1995))
Length = 631
Score = 32.3 bits (70), Expect = 0.23
Identities = 26/75 (34%), Positives = 38/75 (50%)
Frame = +1
Query: 211 MEIEILSVAGSKPLGKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDED 390
M+I I ++ G + +++ TI NVKEKI VK + PD+Q L G+ L D
Sbjct: 552 MQIFIKTLTGKTII--LEVESSDTIANVKEKI--QVKEGIKPDQQ--MLIFFGQQLEDGV 605
Query: 391 TLKSLNIQNGYKLYV 435
TL +I LY+
Sbjct: 606 TLGDYDIHKKSTLYL 620
Score = 27.5 bits (58), Expect = 6.7
Identities = 19/59 (32%), Positives = 26/59 (44%)
Frame = +1
Query: 259 IQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDEDTLKSLNIQNGYKLYV 435
+++ TI NVK K+ + + R L GK L TL NIQ G LY+
Sbjct: 257 LEVESSDTIDNVKAKLQDKERIPMDLHR----LIFAGKPLEGGRTLAHYNIQKGSTLYL 311
>At1g32750.1 68414.m04038 HAC13 protein (HAC13) identical to HAC13
[Arabidopsis thaliana] gi|21105767|gb|AAM34782; contains
Pfam domains, PF00439: Bromodomain and PF00240:
Ubiquitin family
Length = 1919
Score = 31.9 bits (69), Expect = 0.31
Identities = 18/68 (26%), Positives = 38/68 (55%)
Frame = +1
Query: 211 MEIEILSVAGSKPLGKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDED 390
M+I + S+ G K+ + E ++ +++ K + + + +A+K+ KGK L+DE
Sbjct: 661 MKIIVKSLGGKG--SKLHVGIEESVSSLRAKASRKLD---FKETEAVKMFYKGKELDDEK 715
Query: 391 TLKSLNIQ 414
+L + N+Q
Sbjct: 716 SLAAQNVQ 723
>At3g19040.1 68416.m02418 ubiquitin family protein / DNA-binding
bromodomain-containing protein low similarity to
SP|P51123 Transcription initiation factor TFIID 230 kDa
subunit {Drosophila melanogaster}; contains Pfam
profiles: PF00439 bromodomain, PF00240: Ubiquitin family
Length = 1700
Score = 29.9 bits (64), Expect = 1.2
Identities = 17/68 (25%), Positives = 36/68 (52%)
Frame = +1
Query: 211 MEIEILSVAGSKPLGKIQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDED 390
M+I++ ++ G L + + E ++ ++K K + + + +A+K+ GK L DE
Sbjct: 500 MKIKLSNLGGKGSL--LTVGREESVSSLKAKASRKLD---FKETEAVKMFYMGKELEDEK 554
Query: 391 TLKSLNIQ 414
+L N+Q
Sbjct: 555 SLAEQNVQ 562
>At4g25350.1 68417.m03648 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 745
Score = 29.5 bits (63), Expect = 1.7
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +2
Query: 455 YLGRTYFWLNMPGHYSYTFGFTQG 526
Y YFW +YS+ FGF QG
Sbjct: 398 YAANIYFWRRYRVNYSFIFGFKQG 421
>At2g03250.1 68415.m00278 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 756
Score = 29.5 bits (63), Expect = 1.7
Identities = 11/24 (45%), Positives = 13/24 (54%)
Frame = +2
Query: 455 YLGRTYFWLNMPGHYSYTFGFTQG 526
Y YFW +YS+ FGF QG
Sbjct: 411 YAANIYFWRQYRVNYSFIFGFKQG 434
>At2g30100.1 68415.m03663 ubiquitin family protein low similarity to
SP|Q9UQ13 Leucine-rich repeat protein SHOC-2
(Ras-binding protein Sur-8) {Homo sapiens}; contains
Pfam profiles PF00240: Ubiquitin family, PF01535: PPR
repeat, PF00560: Leucine Rich Repeat
Length = 897
Score = 29.1 bits (62), Expect = 2.2
Identities = 18/57 (31%), Positives = 33/57 (57%)
Frame = +1
Query: 259 IQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDEDTLKSLNIQNGYKL 429
+ ++ + T+K++K ++ T++ P Q KL KGK L + TLK ++ +G KL
Sbjct: 549 LSVSPDCTVKDLKSQLQPI--TNVLPRGQ--KLIFKGKVLVETSTLKQSDVGSGAKL 601
>At3g60860.1 68416.m06808 guanine nucleotide exchange family protein
similar to guanine nucleotide exchange factor, Homo
sapiens, GI:5456754; contains Pfam profile PF01369: Sec7
domain
Length = 1793
Score = 28.7 bits (61), Expect = 2.9
Identities = 15/39 (38%), Positives = 20/39 (51%), Gaps = 1/39 (2%)
Frame = -2
Query: 428 SLYPFCMFKDFRVSSSFKVL-PLASNLIACRSGYNEVFT 315
+L C + + K L PL +NLI+C G NEV T
Sbjct: 1737 TLQAMCTLDEASFEKNLKCLFPLLANLISCEHGSNEVQT 1775
>At2g03240.1 68415.m00277 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 823
Score = 28.7 bits (61), Expect = 2.9
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +2
Query: 455 YLGRTYFWLNMPGHYSYTFGFTQG 526
Y G Y+W +YS+ FGF G
Sbjct: 476 YAGNIYYWRRYRVNYSFIFGFKHG 499
>At1g02990.2 68414.m00269 expressed protein similar to
mature-parasite-infected erythrocyte surface antigen
(GI:160409) {Plasmodium falciparum}
Length = 1238
Score = 28.7 bits (61), Expect = 2.9
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +1
Query: 271 EESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDEDTLKSLNIQNGYKLYVKDLGP 450
E+ +I+N+K K K+ YP I+ ++K K+ +E + S + G V+DLG
Sbjct: 807 EDCSIENLKPK-----KSGRYPGENCIEGDSKQKSREEESSAPSKDNNWGLVNNVQDLGT 861
Query: 451 QISWK 465
+ K
Sbjct: 862 AVKVK 866
>At1g02990.1 68414.m00270 expressed protein similar to
mature-parasite-infected erythrocyte surface antigen
(GI:160409) {Plasmodium falciparum}
Length = 1069
Score = 28.7 bits (61), Expect = 2.9
Identities = 19/65 (29%), Positives = 33/65 (50%)
Frame = +1
Query: 271 EESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLNDEDTLKSLNIQNGYKLYVKDLGP 450
E+ +I+N+K K K+ YP I+ ++K K+ +E + S + G V+DLG
Sbjct: 807 EDCSIENLKPK-----KSGRYPGENCIEGDSKQKSREEESSAPSKDNNWGLVNNVQDLGT 861
Query: 451 QISWK 465
+ K
Sbjct: 862 AVKVK 866
>At1g25390.1 68414.m03152 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 629
Score = 28.3 bits (60), Expect = 3.8
Identities = 11/17 (64%), Positives = 11/17 (64%)
Frame = -1
Query: 558 VEVCSPIKIPGPWVNPK 508
V CSPIK P WV PK
Sbjct: 153 VSGCSPIKTPESWVTPK 169
>At1g14040.1 68414.m01660 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 798
Score = 28.3 bits (60), Expect = 3.8
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +2
Query: 455 YLGRTYFWLNMPGHYSYTFGFTQG 526
Y Y+W +YS+ FGF QG
Sbjct: 467 YAANIYYWRRYRVNYSFIFGFKQG 490
>At3g20350.1 68416.m02578 expressed protein
Length = 673
Score = 27.9 bits (59), Expect = 5.0
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +2
Query: 8 LPNLCCTHRERNNITPSGTEY 70
+PN+ TH +N++ +GTEY
Sbjct: 528 IPNISNTHHRNSNVSMNGTEY 548
>At1g69480.1 68414.m07983 EXS family protein / ERD1/XPR1/SYG1 family
protein similar to PHO1 protein [Arabidopsis thaliana]
GI:20069032; contains Pfam profiles PF03105: SPX domain,
PF03124: EXS family
Length = 777
Score = 27.9 bits (59), Expect = 5.0
Identities = 10/24 (41%), Positives = 13/24 (54%)
Frame = +2
Query: 455 YLGRTYFWLNMPGHYSYTFGFTQG 526
Y YFW +Y++ FGF QG
Sbjct: 430 YSANIYFWKRYRVNYTFIFGFKQG 453
>At5g22070.1 68418.m02570 expressed protein contains Pfam profile
PF03267: Arabidopsis protein of unknown function, DUF266
Length = 362
Score = 27.5 bits (58), Expect = 6.7
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -3
Query: 553 SLFSNKDPRPLGKPKGIRIMAR 488
S+F DP P P+GIRI+ R
Sbjct: 184 SIFDKSDPDPNPNPRGIRILYR 205
>At4g28650.1 68417.m04095 leucine-rich repeat transmembrane protein
kinase, putative receptor-like protein kinase 5,
Arabidopsis thaliana, PIR1:S27756
Length = 1013
Score = 27.5 bits (58), Expect = 6.7
Identities = 15/34 (44%), Positives = 24/34 (70%), Gaps = 1/34 (2%)
Frame = +1
Query: 220 EILSVAG-SKPLGKIQLNEESTIKNVKEKIHSSV 318
+++S+ G +KP K NEE+T +++ EK HSSV
Sbjct: 971 DVISMLGEAKPRRKSNSNEENTSRSLAEK-HSSV 1003
>At3g44530.1 68416.m04786 transducin family protein / WD-40 repeat
family protein contains 6 (4 significant) WD-40 repeats
(PF0400); nuclear protein HIRA, mouse, PIR:S68141
Length = 1051
Score = 27.5 bits (58), Expect = 6.7
Identities = 21/76 (27%), Positives = 35/76 (46%), Gaps = 1/76 (1%)
Frame = +1
Query: 208 IMEIEILSVAGSKPLGK-IQLNEESTIKNVKEKIHSSVKTSLYPDRQAIKLEAKGKTLND 384
++E AGSK +Q N+ +T +V + + + S DR E+ G+TLN
Sbjct: 397 LLETASTKQAGSKRAASDVQQNQVTTKPSVSVESTAKRRKSQVDDRNKAA-ESTGQTLNK 455
Query: 385 EDTLKSLNIQNGYKLY 432
TL ++ K+Y
Sbjct: 456 ASTLNRVSSPVNQKVY 471
>At3g16555.1 68416.m02115 F-box family protein contains F-box domain
Pfam:PF00646
Length = 349
Score = 27.5 bits (58), Expect = 6.7
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = +3
Query: 159 LLIQTCNRFQIFWIIYNNGDRNFKRCWFQTSRKNSVK*RIYYKECKRKD 305
L I C+ I WI Y N N + +R V + Y EC+R++
Sbjct: 95 LTITACDGRWIKWIEYENKGFNVCGVGYDNTRPEKVYKILEYLECRREE 143
>At3g18200.1 68416.m02315 nodulin MtN21 family protein similar to
MtN21 GI:2598575 (root nodule development) from
[Medicago truncatula]
Length = 360
Score = 27.1 bits (57), Expect = 8.8
Identities = 11/29 (37%), Positives = 17/29 (58%)
Frame = +1
Query: 454 ISWKNVFLAEYAGPLFVYLWVYPRAWDLY 540
+SW+ +F YAG + L VY + W +Y
Sbjct: 244 VSWEELFTILYAGIIASGLVVYLQTWCIY 272
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,888,639
Number of Sequences: 28952
Number of extensions: 241874
Number of successful extensions: 584
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 574
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 584
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1102220672
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -