BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_I11 (558 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 33 0.17 At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 31 0.39 At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 3.7 At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con... 28 3.7 At2g25730.1 68415.m03084 expressed protein 28 4.9 At1g51380.1 68414.m05780 eukaryotic translation initiation facto... 27 8.5 At1g04120.1 68414.m00401 ABC transporter family protein Strong s... 27 8.5 >At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein similar to beta-hexosaminidase precursor SP:P43077 from [Candida albicans] Length = 580 Score = 32.7 bits (71), Expect = 0.17 Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 3/44 (6%) Frame = +3 Query: 429 YFGMDESYNLXX---XXXXXXXXXXIWGVIRGLETWSQLFYLTN 551 + G+DESY L WG +RGLET+SQ+ + T+ Sbjct: 111 HHGVDESYKLSIPIGSFSAHLLAHSAWGAMRGLETFSQMIWGTS 154 >At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein contains Pfam PF00728: Glycosyl hydrolase family 20, catalytic domain; contains Pfam PF02838: Glycosyl hydrolase family 20, domain 2; similar to Beta-hexosaminidase beta chain precursor (EC 3.2.1.52) (N-acetyl-beta- glucosaminidase) (Beta-N-acetylhexosaminidase) (Hexosaminidase B) (Swiss-Prot:P07686) [Homo sapiens] Length = 535 Score = 31.5 bits (68), Expect = 0.39 Identities = 19/57 (33%), Positives = 30/57 (52%), Gaps = 5/57 (8%) Frame = +3 Query: 381 VQELHVELTAPCEKLPYFGMDESYNLXXXX-----XXXXXXXXIWGVIRGLETWSQL 536 +Q LHV +++ ++L Y G DESY L ++G + GL+T+SQL Sbjct: 102 LQGLHVIISSSTDELEY-GADESYKLVVPSPEKPSYAQLEAKSVYGALHGLQTFSQL 157 >At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|Q13144 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Homo sapiens}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 28.3 bits (60), Expect = 3.7 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +3 Query: 237 FLTKALERYQFLVRDLHRITRRFVK 311 ++ K L R FL+++L +TRRF++ Sbjct: 109 YIQKILRRKPFLIKNLENVTRRFLQ 133 >At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-containing protein low similarity to SP|P47823 Translation initiation factor eIF-2B epsilon subunit (eIF-2B GDP-GTP exchange factor) {Oryctolagus cuniculus}; contains Pfam profile PF02020: eIF4-gamma/eIF5/eIF2-epsilon Length = 411 Score = 28.3 bits (60), Expect = 3.7 Identities = 10/25 (40%), Positives = 18/25 (72%) Frame = +3 Query: 237 FLTKALERYQFLVRDLHRITRRFVK 311 ++ K L R FL+++L +TRRF++ Sbjct: 109 YIQKILRRKAFLIKNLENVTRRFLQ 133 >At2g25730.1 68415.m03084 expressed protein Length = 2464 Score = 27.9 bits (59), Expect = 4.9 Identities = 15/52 (28%), Positives = 26/52 (50%) Frame = +3 Query: 246 KALERYQFLVRDLHRITRRFVKRSLGSQYRSLDDEAANDERFQGTVQELHVE 401 +AL ++ D HR R+F+ L + R+L DE + +G ++ VE Sbjct: 915 RALAMLHQMIEDAHRGKRQFLSGKLHNLARALADEKPEVDVLKGDGSDMAVE 966 >At1g51380.1 68414.m05780 eukaryotic translation initiation factor 4A, putative / eIF-4A, putative Length = 392 Score = 27.1 bits (57), Expect = 8.5 Identities = 12/38 (31%), Positives = 22/38 (57%) Frame = +3 Query: 225 QKCDFLTKALERYQFLVRDLHRITRRFVKRSLGSQYRS 338 QK D+LT+ + F+V +H R+ + + +Q+RS Sbjct: 272 QKVDWLTEKMRSSNFIVSSMHGDKRQKERDDIMNQFRS 309 >At1g04120.1 68414.m00401 ABC transporter family protein Strong similarity to MRP-like ABC transporter gb|U92650 from A. thaliana and canalicular multi-drug resistance protein gb|L49379 from Rattus norvegicus Length = 1514 Score = 27.1 bits (57), Expect = 8.5 Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 5/42 (11%) Frame = -2 Query: 224 EWFLYC-----KYSWVKCIIFIVFHLYLRFRPNLPFCWRILW 114 +WF+ C +W + F+V HL + LPF RI W Sbjct: 109 DWFVLCFPASQSLAWF-VLSFLVLHLKYKSSEKLPFLVRIWW 149 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 13,105,150 Number of Sequences: 28952 Number of extensions: 289892 Number of successful extensions: 637 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 624 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 637 length of database: 12,070,560 effective HSP length: 77 effective length of database: 9,841,256 effective search space used: 1062855648 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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