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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_I11
         (558 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si...    33   0.17 
At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co...    31   0.39 
At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    28   3.7  
At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon domain-con...    28   3.7  
At2g25730.1 68415.m03084 expressed protein                             28   4.9  
At1g51380.1 68414.m05780 eukaryotic translation initiation facto...    27   8.5  
At1g04120.1 68414.m00401 ABC transporter family protein Strong s...    27   8.5  

>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase precursor SP:P43077 from
           [Candida albicans]
          Length = 580

 Score = 32.7 bits (71), Expect = 0.17
 Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 3/44 (6%)
 Frame = +3

Query: 429 YFGMDESYNLXX---XXXXXXXXXXIWGVIRGLETWSQLFYLTN 551
           + G+DESY L                WG +RGLET+SQ+ + T+
Sbjct: 111 HHGVDESYKLSIPIGSFSAHLLAHSAWGAMRGLETFSQMIWGTS 154


>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
           contains Pfam PF00728: Glycosyl hydrolase family 20,
           catalytic domain; contains Pfam PF02838: Glycosyl
           hydrolase family 20, domain 2; similar to
           Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
           (N-acetyl-beta- glucosaminidase)
           (Beta-N-acetylhexosaminidase) (Hexosaminidase B)
           (Swiss-Prot:P07686) [Homo sapiens]
          Length = 535

 Score = 31.5 bits (68), Expect = 0.39
 Identities = 19/57 (33%), Positives = 30/57 (52%), Gaps = 5/57 (8%)
 Frame = +3

Query: 381 VQELHVELTAPCEKLPYFGMDESYNLXXXX-----XXXXXXXXIWGVIRGLETWSQL 536
           +Q LHV +++  ++L Y G DESY L                 ++G + GL+T+SQL
Sbjct: 102 LQGLHVIISSSTDELEY-GADESYKLVVPSPEKPSYAQLEAKSVYGALHGLQTFSQL 157


>At5g36230.1 68418.m04371 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|Q13144
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Homo sapiens};
           contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 28.3 bits (60), Expect = 3.7
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +3

Query: 237 FLTKALERYQFLVRDLHRITRRFVK 311
           ++ K L R  FL+++L  +TRRF++
Sbjct: 109 YIQKILRRKPFLIKNLENVTRRFLQ 133


>At1g65220.1 68414.m07394 eIF4-gamma/eIF5/eIF2-epsilon
           domain-containing protein low similarity to SP|P47823
           Translation initiation factor eIF-2B epsilon subunit
           (eIF-2B GDP-GTP exchange factor) {Oryctolagus
           cuniculus}; contains Pfam profile PF02020:
           eIF4-gamma/eIF5/eIF2-epsilon
          Length = 411

 Score = 28.3 bits (60), Expect = 3.7
 Identities = 10/25 (40%), Positives = 18/25 (72%)
 Frame = +3

Query: 237 FLTKALERYQFLVRDLHRITRRFVK 311
           ++ K L R  FL+++L  +TRRF++
Sbjct: 109 YIQKILRRKAFLIKNLENVTRRFLQ 133


>At2g25730.1 68415.m03084 expressed protein 
          Length = 2464

 Score = 27.9 bits (59), Expect = 4.9
 Identities = 15/52 (28%), Positives = 26/52 (50%)
 Frame = +3

Query: 246  KALERYQFLVRDLHRITRRFVKRSLGSQYRSLDDEAANDERFQGTVQELHVE 401
            +AL     ++ D HR  R+F+   L +  R+L DE    +  +G   ++ VE
Sbjct: 915  RALAMLHQMIEDAHRGKRQFLSGKLHNLARALADEKPEVDVLKGDGSDMAVE 966


>At1g51380.1 68414.m05780 eukaryotic translation initiation factor
           4A, putative / eIF-4A, putative
          Length = 392

 Score = 27.1 bits (57), Expect = 8.5
 Identities = 12/38 (31%), Positives = 22/38 (57%)
 Frame = +3

Query: 225 QKCDFLTKALERYQFLVRDLHRITRRFVKRSLGSQYRS 338
           QK D+LT+ +    F+V  +H   R+  +  + +Q+RS
Sbjct: 272 QKVDWLTEKMRSSNFIVSSMHGDKRQKERDDIMNQFRS 309


>At1g04120.1 68414.m00401 ABC transporter family protein Strong
           similarity to MRP-like ABC transporter gb|U92650 from A.
           thaliana and canalicular multi-drug resistance protein
           gb|L49379 from Rattus norvegicus
          Length = 1514

 Score = 27.1 bits (57), Expect = 8.5
 Identities = 14/42 (33%), Positives = 20/42 (47%), Gaps = 5/42 (11%)
 Frame = -2

Query: 224 EWFLYC-----KYSWVKCIIFIVFHLYLRFRPNLPFCWRILW 114
           +WF+ C       +W   + F+V HL  +    LPF  RI W
Sbjct: 109 DWFVLCFPASQSLAWF-VLSFLVLHLKYKSSEKLPFLVRIWW 149


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,105,150
Number of Sequences: 28952
Number of extensions: 289892
Number of successful extensions: 637
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 624
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 637
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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