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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_H23
         (533 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po...   221   7e-59
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po...   208   5e-55
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb...    27   1.8  
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb...    27   1.8  
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce...    26   3.1  
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce...    25   5.4  
SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1...    25   5.4  
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz...    25   5.4  
SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c...    25   7.1  
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb...    25   7.1  
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S...    25   7.1  
SPBP35G2.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2|...    25   7.1  
SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz...    25   9.4  
SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein L18|S...    25   9.4  

>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  221 bits (539), Expect = 7e-59
 Identities = 102/141 (72%), Positives = 120/141 (85%)
 Frame = +3

Query: 6   ICYDVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDA 185
           IC D+L +L+KHLIP A++ ESKVFY KMKGDYYRYLAE A GE R    + S + Y+ A
Sbjct: 98  ICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAA 157

Query: 186 FEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYK 365
            EI+ A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LAKQAFD+AI+ELD+L+E+SYK
Sbjct: 158 SEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYK 217

Query: 366 DSTLIMQLLRDNLTLWTSDTQ 428
           DSTLIMQLLRDNLTLWTSD +
Sbjct: 218 DSTLIMQLLRDNLTLWTSDAE 238


>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 270

 Score =  208 bits (507), Expect = 5e-55
 Identities = 96/142 (67%), Positives = 117/142 (82%)
 Frame = +3

Query: 3   EICYDVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQD 182
           +IC+DVL +L+KHLIP A+  ESKVFY KMKGDYYRYLAE   GE      + S +AY+ 
Sbjct: 96  DICHDVLSVLEKHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKA 155

Query: 183 AFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSY 362
           A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD+AI+ELD+L+E+SY
Sbjct: 156 ASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESY 215

Query: 363 KDSTLIMQLLRDNLTLWTSDTQ 428
           KDSTLIMQLLRDNLTLWTSD +
Sbjct: 216 KDSTLIMQLLRDNLTLWTSDAE 237


>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
            2|||Manual
          Length = 1207

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
 Frame = +3

Query: 108  RYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 254
            RY+  ++   E R    ED+++A + ++  + A +QP H  I L LA+N S
Sbjct: 1049 RYIVALSCELERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099


>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1778

 Score = 27.1 bits (57), Expect = 1.8
 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
 Frame = +3

Query: 27   LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 164
            +++K  IP++   E+K  Y +  GD+     +L E A  E  H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656


>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 817

 Score = 26.2 bits (55), Expect = 3.1
 Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 2/81 (2%)
 Frame = +3

Query: 165 QKAYQDAFEISKAKMQPTHPIRL-GLALNF-SVFYYEILNSPDKACQLAKQAFDDAIAEL 338
           Q+   D  E   +   P H        L F S  Y ++    D   QLA Q F     ++
Sbjct: 415 QRLVADLHEFYNSVKGPAHSFETEDPVLKFVSQSYDDLFPYMDNFIQLAVQLFYHISKKV 474

Query: 339 DTLNEDSYKDSTLIMQLLRDN 401
           + L  +++  S  ++Q ++DN
Sbjct: 475 NCLYVEAFSSSDAVLQRIQDN 495


>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 844

 Score = 25.4 bits (53), Expect = 5.4
 Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 3/36 (8%)
 Frame = +3

Query: 18  VLCLLDKHLIPKASN-PESKVFYLKMKGD--YYRYL 116
           VL  L+  L+  A+  PE K+FY K  GD  Y +YL
Sbjct: 509 VLMQLESFLLNIANPAPEGKLFYEKQTGDNPYLKYL 544


>SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 85

 Score = 25.4 bits (53), Expect = 5.4
 Identities = 13/34 (38%), Positives = 19/34 (55%)
 Frame = -1

Query: 218 CGLHFCLADLKSILVCFL*ILYNRMSGFSCGHFC 117
           C L+F L + K+ L+    I+Y  + GFS  H C
Sbjct: 46  CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75


>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 603

 Score = 25.4 bits (53), Expect = 5.4
 Identities = 8/21 (38%), Positives = 14/21 (66%)
 Frame = -1

Query: 128 GHFCEIPVVISLHFEVKYFTF 66
           G+FC++P V+ L    K++ F
Sbjct: 341 GNFCDLPKVVELKNRYKFYLF 361


>SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 559

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
 Frame = -1

Query: 224 WVCGLHFCLADLKSILVCFL*ILYNRMSGF-SCGHFCEIPVVISLHFEVKYFTFWITSFR 48
           ++ GL +   ++ +   C   ILY     F S  +       I+  F + YF +W+ SF 
Sbjct: 152 FIAGLFYYGTNVYTGAECVQTILYAIFKSFRSYKNRLPADAGITSDFLISYFVYWVISFP 211

Query: 47  YKVL 36
           + ++
Sbjct: 212 FHLI 215


>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 620

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 21/83 (25%), Positives = 33/83 (39%)
 Frame = +3

Query: 15  DVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEI 194
           DV+C  +  L+P    PE K+FY             + +G    S    SQ+   D +EI
Sbjct: 178 DVICGKEAFLLPLNEYPEYKLFY------------PIKSGVFNESDYASSQQLLADIYEI 225

Query: 195 SKAKMQPTHPIRLGLALNFSVFY 263
            K  +     I +     +SV +
Sbjct: 226 FKYSITSLLQIPVSQLSQYSVIF 248


>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 971

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 13/42 (30%), Positives = 18/42 (42%)
 Frame = +3

Query: 39  HLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS 164
           H     SNP S     K KG++  Y    A G    ++ +DS
Sbjct: 225 HTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDS 266


>SPBP35G2.04c |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 249

 Score = 25.0 bits (52), Expect = 7.1
 Identities = 10/20 (50%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
 Frame = -1

Query: 113 IPVVISLHFEVKYFT-FWIT 57
           +P++I LHF  K FT  W+T
Sbjct: 62  LPILIRLHFYKKLFTILWLT 81


>SPAC3A11.05c |kms1||meiotic spindle pole body protein
           Kms1|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 607

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 14/40 (35%), Positives = 19/40 (47%)
 Frame = +3

Query: 33  DKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSV 152
           +KHLI  ++NP+   F      D   Y  EV    T+ SV
Sbjct: 354 EKHLIALSNNPKGDEFLSPSNLDEMVYSKEVGLSFTQPSV 393


>SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein
           L18|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 187

 Score = 24.6 bits (51), Expect = 9.4
 Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 2/32 (6%)
 Frame = +3

Query: 30  LDKHLIPKA--SNPESKVFYLKMKGDYYRYLA 119
           +++H + K+  S P S+  YLK+    YR+LA
Sbjct: 5   IERHHVKKSQRSKPASENVYLKLLVKLYRFLA 36


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,907,479
Number of Sequences: 5004
Number of extensions: 36139
Number of successful extensions: 110
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 110
length of database: 2,362,478
effective HSP length: 68
effective length of database: 2,022,206
effective search space used: 220420454
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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