BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_H23 (533 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 221 7e-59 SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 208 5e-55 SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 1.8 SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 1.8 SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 26 3.1 SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 25 5.4 SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 5.4 SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 25 5.4 SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c... 25 7.1 SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 25 7.1 SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 25 7.1 SPBP35G2.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 7.1 SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schiz... 25 9.4 SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein L18|S... 25 9.4 >SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 221 bits (539), Expect = 7e-59 Identities = 102/141 (72%), Positives = 120/141 (85%) Frame = +3 Query: 6 ICYDVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDA 185 IC D+L +L+KHLIP A++ ESKVFY KMKGDYYRYLAE A GE R + S + Y+ A Sbjct: 98 ICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAA 157 Query: 186 FEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSYK 365 EI+ A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LAKQAFD+AI+ELD+L+E+SYK Sbjct: 158 SEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYK 217 Query: 366 DSTLIMQLLRDNLTLWTSDTQ 428 DSTLIMQLLRDNLTLWTSD + Sbjct: 218 DSTLIMQLLRDNLTLWTSDAE 238 >SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces pombe|chr 1|||Manual Length = 270 Score = 208 bits (507), Expect = 5e-55 Identities = 96/142 (67%), Positives = 117/142 (82%) Frame = +3 Query: 3 EICYDVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQD 182 +IC+DVL +L+KHLIP A+ ESKVFY KMKGDYYRYLAE GE + S +AY+ Sbjct: 96 DICHDVLSVLEKHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKA 155 Query: 183 AFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTLNEDSY 362 A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD+AI+ELD+L+E+SY Sbjct: 156 ASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSLSEESY 215 Query: 363 KDSTLIMQLLRDNLTLWTSDTQ 428 KDSTLIMQLLRDNLTLWTSD + Sbjct: 216 KDSTLIMQLLRDNLTLWTSDAE 237 >SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr 2|||Manual Length = 1207 Score = 27.1 bits (57), Expect = 1.8 Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 2/51 (3%) Frame = +3 Query: 108 RYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 254 RY+ ++ E R ED+++A + ++ + A +QP H I L LA+N S Sbjct: 1049 RYIVALSCELERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099 >SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr 1|||Manual Length = 1778 Score = 27.1 bits (57), Expect = 1.8 Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%) Frame = +3 Query: 27 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 164 +++K IP++ E+K Y + GD+ +L E A E H V+ D+ Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656 >SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 817 Score = 26.2 bits (55), Expect = 3.1 Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 2/81 (2%) Frame = +3 Query: 165 QKAYQDAFEISKAKMQPTHPIRL-GLALNF-SVFYYEILNSPDKACQLAKQAFDDAIAEL 338 Q+ D E + P H L F S Y ++ D QLA Q F ++ Sbjct: 415 QRLVADLHEFYNSVKGPAHSFETEDPVLKFVSQSYDDLFPYMDNFIQLAVQLFYHISKKV 474 Query: 339 DTLNEDSYKDSTLIMQLLRDN 401 + L +++ S ++Q ++DN Sbjct: 475 NCLYVEAFSSSDAVLQRIQDN 495 >SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces pombe|chr 1|||Manual Length = 844 Score = 25.4 bits (53), Expect = 5.4 Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 3/36 (8%) Frame = +3 Query: 18 VLCLLDKHLIPKASN-PESKVFYLKMKGD--YYRYL 116 VL L+ L+ A+ PE K+FY K GD Y +YL Sbjct: 509 VLMQLESFLLNIANPAPEGKLFYEKQTGDNPYLKYL 544 >SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 85 Score = 25.4 bits (53), Expect = 5.4 Identities = 13/34 (38%), Positives = 19/34 (55%) Frame = -1 Query: 218 CGLHFCLADLKSILVCFL*ILYNRMSGFSCGHFC 117 C L+F L + K+ L+ I+Y + GFS H C Sbjct: 46 CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75 >SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 603 Score = 25.4 bits (53), Expect = 5.4 Identities = 8/21 (38%), Positives = 14/21 (66%) Frame = -1 Query: 128 GHFCEIPVVISLHFEVKYFTF 66 G+FC++P V+ L K++ F Sbjct: 341 GNFCDLPKVVELKNRYKFYLF 361 >SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 559 Score = 25.0 bits (52), Expect = 7.1 Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 1/64 (1%) Frame = -1 Query: 224 WVCGLHFCLADLKSILVCFL*ILYNRMSGF-SCGHFCEIPVVISLHFEVKYFTFWITSFR 48 ++ GL + ++ + C ILY F S + I+ F + YF +W+ SF Sbjct: 152 FIAGLFYYGTNVYTGAECVQTILYAIFKSFRSYKNRLPADAGITSDFLISYFVYWVISFP 211 Query: 47 YKVL 36 + ++ Sbjct: 212 FHLI 215 >SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pombe|chr 1|||Manual Length = 620 Score = 25.0 bits (52), Expect = 7.1 Identities = 21/83 (25%), Positives = 33/83 (39%) Frame = +3 Query: 15 DVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEI 194 DV+C + L+P PE K+FY + +G S SQ+ D +EI Sbjct: 178 DVICGKEAFLLPLNEYPEYKLFY------------PIKSGVFNESDYASSQQLLADIYEI 225 Query: 195 SKAKMQPTHPIRLGLALNFSVFY 263 K + I + +SV + Sbjct: 226 FKYSITSLLQIPVSQLSQYSVIF 248 >SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |Schizosaccharomyces pombe|chr 1|||Manual Length = 971 Score = 25.0 bits (52), Expect = 7.1 Identities = 13/42 (30%), Positives = 18/42 (42%) Frame = +3 Query: 39 HLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS 164 H SNP S K KG++ Y A G ++ +DS Sbjct: 225 HTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDS 266 >SPBP35G2.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 249 Score = 25.0 bits (52), Expect = 7.1 Identities = 10/20 (50%), Positives = 14/20 (70%), Gaps = 1/20 (5%) Frame = -1 Query: 113 IPVVISLHFEVKYFT-FWIT 57 +P++I LHF K FT W+T Sbjct: 62 LPILIRLHFYKKLFTILWLT 81 >SPAC3A11.05c |kms1||meiotic spindle pole body protein Kms1|Schizosaccharomyces pombe|chr 1|||Manual Length = 607 Score = 24.6 bits (51), Expect = 9.4 Identities = 14/40 (35%), Positives = 19/40 (47%) Frame = +3 Query: 33 DKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSV 152 +KHLI ++NP+ F D Y EV T+ SV Sbjct: 354 EKHLIALSNNPKGDEFLSPSNLDEMVYSKEVGLSFTQPSV 393 >SPBC11C11.07 |rpl1801|rpl18-1, rpl18|60S ribosomal protein L18|Schizosaccharomyces pombe|chr 2|||Manual Length = 187 Score = 24.6 bits (51), Expect = 9.4 Identities = 12/32 (37%), Positives = 20/32 (62%), Gaps = 2/32 (6%) Frame = +3 Query: 30 LDKHLIPKA--SNPESKVFYLKMKGDYYRYLA 119 +++H + K+ S P S+ YLK+ YR+LA Sbjct: 5 IERHHVKKSQRSKPASENVYLKLLVKLYRFLA 36 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,907,479 Number of Sequences: 5004 Number of extensions: 36139 Number of successful extensions: 110 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 110 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 110 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 220420454 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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