BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_H20 (512 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 34 0.049 At3g02280.1 68416.m00209 flavodoxin family protein low similarit... 29 2.4 At1g70900.1 68414.m08181 expressed protein 29 2.4 At1g23110.1 68414.m02889 hypothetical protein 29 2.4 At5g19010.1 68418.m02258 mitogen-activated protein kinase, putat... 28 4.2 At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 28 4.2 At3g25140.1 68416.m03139 glycosyl transferase family 8 protein c... 28 4.2 At5g35940.1 68418.m04325 jacalin lectin family protein similar t... 27 5.6 At4g30550.1 68417.m04336 glutamine amidotransferase class-I doma... 27 5.6 At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 27 5.6 At2g20170.1 68415.m02358 hypothetical protein and grail contain... 27 5.6 At5g59920.1 68418.m07514 DC1 domain-containing protein contains ... 27 7.4 At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identic... 27 7.4 At2g23990.2 68415.m02866 plastocyanin-like domain-containing pro... 27 7.4 At2g23990.1 68415.m02865 plastocyanin-like domain-containing pro... 27 7.4 At1g76740.1 68414.m08931 expressed protein weak similarity to fi... 27 9.8 >At2g26390.1 68415.m03167 serpin, putative / serine protease inhibitor, putative similar to phloem serpin-1 [Cucurbita maxima] GI:9937311; contains Pfam profile PF00079: Serpin (serine protease inhibitor) Length = 389 Score = 34.3 bits (75), Expect = 0.049 Identities = 27/114 (23%), Positives = 53/114 (46%), Gaps = 3/114 (2%) Frame = +3 Query: 180 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 359 NVV SP+ + +L+ L AG+ ++ EI FL + + ++ +E + + Sbjct: 31 NVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDHLNAVLAKIADGGTERSDLCLS 90 Query: 360 MANKIYVGNKYTLDEKF-TITVRQYQSEVETIDFSDTK--KAADIINQWANEKT 512 A+ +++ L F + Y++ +DF+ TK + D +N WA+ T Sbjct: 91 TAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFA-TKPVEVIDEVNIWADVHT 143 >At3g02280.1 68416.m00209 flavodoxin family protein low similarity to SP|Q05001 NADPH-cytochrome P450 reductase (EC 1.6.2.4) {Catharanthus roseus}, similar to NADPH-dependent FMN and FAD containing oxidoreductase [Homo sapiens] GI:6694369; contains Pfam profiles PF00258: flavodoxin, PF00667: FAD binding domain, PF00175: Oxidoreductase NAD-binding domain Length = 623 Score = 28.7 bits (61), Expect = 2.4 Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 7/80 (8%) Frame = +3 Query: 246 GSRAEIDKFLGNGD----YSGVANPY-ISLSKTFSEMNPDYFTMANKIYVGNKYTLDE-K 407 G+ I+K LG+ Y G +P+ +SL +T ++NP YF + + +D+ K Sbjct: 123 GATTIIEKGLGDDQHPSGYEGTLDPWMLSLWRTLYQINPKYFPKGPDVKIPQDEVIDKPK 182 Query: 408 FTITV-RQYQSEVETIDFSD 464 + I +Q + E + + SD Sbjct: 183 YRILFHKQEKLEPKLLSDSD 202 >At1g70900.1 68414.m08181 expressed protein Length = 244 Score = 28.7 bits (61), Expect = 2.4 Identities = 19/66 (28%), Positives = 32/66 (48%) Frame = +3 Query: 177 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 356 K +S +GV + LY + SR + K+L DY+ +A I L++ E NP + Sbjct: 103 KVYANSLIGVGIASSLYHS-----SRGKFRKYLRWADYTMIATATICLTRALREENPKFL 157 Query: 357 TMANKI 374 A+ + Sbjct: 158 MAASAL 163 >At1g23110.1 68414.m02889 hypothetical protein Length = 261 Score = 28.7 bits (61), Expect = 2.4 Identities = 20/66 (30%), Positives = 32/66 (48%) Frame = +3 Query: 177 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 356 K +S +GV + LY A SR ++ K+L DY+ +A I LS+ NP + Sbjct: 120 KVYANSLIGVGIASSLYHA-----SRGKLRKYLRWVDYTMIATTTICLSRALRNENPKFL 174 Query: 357 TMANKI 374 A+ + Sbjct: 175 MAASAL 180 >At5g19010.1 68418.m02258 mitogen-activated protein kinase, putative / MAPK, putative (MPK16) mitogen-activated protein kinase (MAPK)(AtMPK16), PMID:12119167; similar to ATMPK9, Arabidopsis thaliana, EMBL:AB038694 Length = 567 Score = 27.9 bits (59), Expect = 4.2 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 240 GEGSRAEIDKFLGNGDYSGVANPY 311 GEGSR I++ +G G Y V + Y Sbjct: 20 GEGSRYRIEEVIGKGSYGVVCSAY 43 >At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein similar to alpha-mannosidase II SP:P27046 from [Mus musculus] Length = 1173 Score = 27.9 bits (59), Expect = 4.2 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = -2 Query: 229 LYRSISIITPNGEDTTFLSFDDVYVSLRN 143 LYR+ +++ P G+D ++S D+ RN Sbjct: 401 LYRTNTLLIPLGDDFRYISIDEAEAQFRN 429 >At3g25140.1 68416.m03139 glycosyl transferase family 8 protein contains Pfam profile: PF01501 glycosyl transferase family 8 Length = 559 Score = 27.9 bits (59), Expect = 4.2 Identities = 17/77 (22%), Positives = 35/77 (45%) Frame = +3 Query: 180 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 359 +VV+ + + + +++K +G+ E+ DY+ + + Y+ + K N F Sbjct: 281 HVVTDKMNLGAMQVMFKLKEYKGAHVEVKAV---EDYTFLNSSYVPVLKQLESANLQKFY 337 Query: 360 MANKIYVGNKYTLDEKF 410 NK+ K T + KF Sbjct: 338 FENKLENATKDTTNMKF 354 >At5g35940.1 68418.m04325 jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI:2997767; contains Pfam profile PF01419 jacalin-like lectin domain Length = 444 Score = 27.5 bits (58), Expect = 5.6 Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 1/61 (1%) Frame = +3 Query: 174 DKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKT-FSEMNPD 350 DKNV S LG + +L +G + + GDY G+ Y++ T M D Sbjct: 131 DKNVYS--LGAYFIKVLATKSGMQGGQNTGKSYDYGGDYDGIRKVYVTYDGTSIRHMRVD 188 Query: 351 Y 353 Y Sbjct: 189 Y 189 >At4g30550.1 68417.m04336 glutamine amidotransferase class-I domain-containing protein similar to defense-related protein [Brassica carinata] GI:14009290, component of aniline dioxygenase (GMP synthase like protein) - Acinetobacter sp.,PID:d1013698; contains Pfam profile PF00117: glutamine amidotransferase class-I Length = 249 Score = 27.5 bits (58), Expect = 5.6 Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 8/60 (13%) Frame = +3 Query: 39 LLAVIAVCNVRAFYSSDH--------EYNRTALGDAIDKTSLQFLKETYTSSKDKNVVSS 194 LLA VCNV F DH EYN+ L + +D+ L E + K K+ + + Sbjct: 172 LLASSEVCNVEMFSIGDHFFCIQGHPEYNKEILFEIVDRVLNMKLMEQEFADKAKSTMET 231 >At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing protein low similarity to nucleolar phosphoprotein (Nopp52), Tetrahymena thermophila, EMBL:TT51555; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 597 Score = 27.5 bits (58), Expect = 5.6 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = +3 Query: 264 DKFLGNGDYSGVANPYISLSKTFSEMNPDYFTMANKIYVGN 386 D++L Y P I KT S P+ N++Y+GN Sbjct: 228 DRYLTIQQYVKTTTPSIPRRKTSSGFAPEMVDGYNRVYIGN 268 >At2g20170.1 68415.m02358 hypothetical protein and grail contains Pfam profile PF03080: Arabidopsis proteins of unknown function Length = 401 Score = 27.5 bits (58), Expect = 5.6 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +3 Query: 102 RTALGDAIDKTSLQFLKETYTSSKDKNVVS 191 RT L D I L++L YT+SKDK+ ++ Sbjct: 121 RTTLEDLIQIQRLKYLGVKYTTSKDKDFLN 150 >At5g59920.1 68418.m07514 DC1 domain-containing protein contains Pfam profile PF03107: DC1 domain Length = 710 Score = 27.1 bits (57), Expect = 7.4 Identities = 15/41 (36%), Positives = 20/41 (48%) Frame = +2 Query: 95 VQQDSLRRCH*QNFFAVS*GNVYIIEGQECCVFTIRRDDAN 217 V D RRC V G++Y+ E +ECC F + AN Sbjct: 384 VIHDERRRCE-ACIHPVQFGSIYVCEEEECC-FVLHEKCAN 422 >At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identical to AtSPO11-1 [Arabidopsis thaliana] GI:13383478; contains Pfam profile PF04406: Type IIB DNA topoisomerase; identical to cDNA putative topoisomerase VIA (SPO11 gene 1) GI:7270974 Length = 362 Score = 27.1 bits (57), Expect = 7.4 Identities = 13/27 (48%), Positives = 16/27 (59%) Frame = +3 Query: 117 DAIDKTSLQFLKETYTSSKDKNVVSSP 197 +A+ SL FL E Y SK + VSSP Sbjct: 336 EALSVHSLSFLSEVYIPSKIRREVSSP 362 >At2g23990.2 68415.m02866 plastocyanin-like domain-containing protein Length = 226 Score = 27.1 bits (57), Expect = 7.4 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = -2 Query: 286 SPLPKNLSISARLPSPAPALYRSISIITPNG 194 +P PK+ S S P+PAPA ++ ++ NG Sbjct: 181 TPSPKS-STSTMAPAPAPAKSSAVGLVAGNG 210 >At2g23990.1 68415.m02865 plastocyanin-like domain-containing protein Length = 207 Score = 27.1 bits (57), Expect = 7.4 Identities = 12/31 (38%), Positives = 19/31 (61%) Frame = -2 Query: 286 SPLPKNLSISARLPSPAPALYRSISIITPNG 194 +P PK+ S S P+PAPA ++ ++ NG Sbjct: 162 TPSPKS-STSTMAPAPAPAKSSAVGLVAGNG 191 >At1g76740.1 68414.m08931 expressed protein weak similarity to fimbriae-associated protein Fap1 (GI:3929312) [Streptococcus parasanguinis]; weak similarity to 1MDa_1 protein (GI:24620455) [Caenorhabditis elegans] Length = 1532 Score = 26.6 bits (56), Expect = 9.8 Identities = 28/97 (28%), Positives = 41/97 (42%), Gaps = 2/97 (2%) Frame = +3 Query: 216 MLLYKAGAGEGSRAEIDKFLGNGDYSGVANPY-ISLSKTFSEMNPDYFTMANKIYVGNKY 392 +LL +G G E G D A P I+ K S + PD T+ N + N+ Sbjct: 1383 LLLNSSGGGSNVSGETSILAGTADEDEAAGPIAIAFVKAMS-LTPDELTVKNHM---NEK 1438 Query: 393 TLDEKFTITVRQYQSEVETI-DFSDTKKAADIINQWA 500 DE+ IT ETI + S+ +K I++ A Sbjct: 1439 AADEEM-ITRETTPIPEETINESSENRKEYSSIDESA 1474 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,785,264 Number of Sequences: 28952 Number of extensions: 224252 Number of successful extensions: 686 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 670 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 686 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 927799552 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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