Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_H15
(568 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g58350.1 68414.m06637 expressed protein contains Pfam profile... 83 1e-16
At1g09980.1 68414.m01126 expressed protein contains Pfam profile... 78 4e-15
At1g10040.1 68414.m01132 expressed protein non-consensus GC dono... 56 2e-08
At4g25770.1 68417.m03709 expressed protein 50 1e-06
At1g29120.2 68414.m03565 expressed protein 50 1e-06
At1g29120.1 68414.m03564 expressed protein 50 1e-06
At5g51180.2 68418.m06346 expressed protein 49 2e-06
At5g51180.1 68418.m06345 expressed protein 49 2e-06
At1g73750.1 68414.m08539 expressed protein contains Pfam profile... 30 1.2
At1g63990.1 68414.m07248 DNA topoisomerase VIA, putative (SPO11-... 30 1.2
At3g46930.1 68416.m05093 protein kinase family protein contains ... 29 1.6
At1g18620.1 68414.m02321 expressed protein 29 1.6
At1g02660.1 68414.m00216 lipase class 3 family protein contains ... 29 1.6
At1g15060.1 68414.m01800 expressed protein 29 2.9
At3g61680.1 68416.m06912 lipase class 3 family protein contains ... 28 3.8
At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein s... 28 3.8
At1g65440.1 68414.m07424 glycine-rich protein 28 5.0
At3g52900.1 68416.m05830 expressed protein contains Pfam profile... 27 6.6
At2g44810.1 68415.m05576 defective in anther dehiscence1 (DAD1) ... 27 6.6
At1g63210.1 68414.m07144 hypothetical protein contains Pfam prof... 27 6.6
At1g26680.1 68414.m03250 transcriptional factor B3 family protei... 27 6.6
At5g01970.1 68418.m00116 expressed protein 27 8.7
At1g09530.2 68414.m01069 phytochrome interacting factor 3 (PIF3)... 27 8.7
At1g09530.1 68414.m01068 phytochrome interacting factor 3 (PIF3)... 27 8.7
>At1g58350.1 68414.m06637 expressed protein contains Pfam profile
PF05057: Protein of unknown function (DUF676);
supporting cDNA gi|6520166|dbj|AB028199.1|
Length = 794
Score = 83.0 bits (196), Expect = 1e-16
Identities = 47/116 (40%), Positives = 70/116 (60%), Gaps = 9/116 (7%)
Frame = +2
Query: 248 LHLIICVHGLDGNSGDLRLVKTYLELGLPGARLDFLMSERNQGDTFSDFDTMTDRLVQEI 427
L +++ VHG G+ DLRLV+ L P +++FLMSE N+ T DF M RL QE+
Sbjct: 512 LKIVVFVHGFQGHHLDLRLVRNQWLLIDP--KIEFLMSEANEEKTHGDFREMGQRLAQEV 569
Query: 428 VTHIQNSSE---------PARISFVGHSLGTIIIRSALARAQMKAYLGKLHTFLSL 568
V+ ++ + ++SFVGHS+G +IIR+A+A + M+ Y LHT+LSL
Sbjct: 570 VSFLKRKKDRYARQGHLKSIKLSFVGHSIGNVIIRTAIADSLMEPYRKYLHTYLSL 625
>At1g09980.1 68414.m01126 expressed protein contains Pfam profile
PF05057: Protein of unknown function (DUF676);
non-consensus GC donor splice site at exon boundary
144764
Length = 802
Score = 78.2 bits (184), Expect = 4e-15
Identities = 44/116 (37%), Positives = 67/116 (57%), Gaps = 9/116 (7%)
Frame = +2
Query: 248 LHLIICVHGLDGNSGDLRLVKTYLELGLPGARLDFLMSERNQGDTFSDFDTMTDRLVQEI 427
L +++ VHG G+ DLRL++ L P +++FLMSE N+ T DF M RL QE+
Sbjct: 520 LKIVVFVHGFQGHHLDLRLIRNQWLLIDP--KIEFLMSEANEERTHGDFREMGQRLAQEV 577
Query: 428 VTHIQNSSEP---------ARISFVGHSLGTIIIRSALARAQMKAYLGKLHTFLSL 568
V+ + + ++SFVGHS+G +IIR+A+A + M Y HT++SL
Sbjct: 578 VSFFKRKKDKHARYGRLKNIKLSFVGHSIGNVIIRAAIADSLMDPYRKYFHTYISL 633
>At1g10040.1 68414.m01132 expressed protein non-consensus GC donor
splice site at exon boundary 21576
Length = 412
Score = 55.6 bits (128), Expect = 2e-08
Identities = 31/93 (33%), Positives = 47/93 (50%)
Frame = +2
Query: 242 EGLHLIICVHGLDGNSGDLRLVKTYLELGLPGARLDFLMSERNQGDTFSDFDTMTDRLVQ 421
E HL++ V+GL G++ + R + P L + TF D M +RL +
Sbjct: 78 EPTHLVVMVNGLIGSAQNWRFAAKQMLKKYPQDLLVHCSKRNHSTQTFDGVDVMGERLAE 137
Query: 422 EIVTHIQNSSEPARISFVGHSLGTIIIRSALAR 520
E+ + I+ +ISFVGHSLG +I R A+ R
Sbjct: 138 EVRSVIKRHPSLQKISFVGHSLGGLIARYAIGR 170
>At4g25770.1 68417.m03709 expressed protein
Length = 418
Score = 49.6 bits (113), Expect = 1e-06
Identities = 26/90 (28%), Positives = 45/90 (50%)
Frame = +2
Query: 251 HLIICVHGLDGNSGDLRLVKTYLELGLPGARLDFLMSERNQGDTFSDFDTMTDRLVQEIV 430
HL++ V+G+ G++ D + P L + TF D M +RL E++
Sbjct: 93 HLVVMVNGIVGSAADWKYAAEQFVKKFPDKVLVHRSESNSATLTFDGVDKMGERLANEVL 152
Query: 431 THIQNSSEPARISFVGHSLGTIIIRSALAR 520
+++ S +ISFV HSLG ++ R A+ +
Sbjct: 153 GVVKHRSGLKKISFVAHSLGGLVARYAIGK 182
>At1g29120.2 68414.m03565 expressed protein
Length = 455
Score = 49.6 bits (113), Expect = 1e-06
Identities = 29/89 (32%), Positives = 43/89 (48%)
Frame = +2
Query: 251 HLIICVHGLDGNSGDLRLVKTYLELGLPGARLDFLMSERNQGDTFSDFDTMTDRLVQEIV 430
HL++ VHG+ + D V+ L+ L L + S TF D RL +E+
Sbjct: 102 HLLVLVHGILASPSDWLYVEAELKRRLGRRFLIYASSSNTFTKTFGGIDGAGKRLAEEVR 161
Query: 431 THIQNSSEPARISFVGHSLGTIIIRSALA 517
+Q S +ISF+ HSLG + R A+A
Sbjct: 162 QVVQKSKSLKKISFLAHSLGGLFSRHAVA 190
>At1g29120.1 68414.m03564 expressed protein
Length = 455
Score = 49.6 bits (113), Expect = 1e-06
Identities = 29/89 (32%), Positives = 43/89 (48%)
Frame = +2
Query: 251 HLIICVHGLDGNSGDLRLVKTYLELGLPGARLDFLMSERNQGDTFSDFDTMTDRLVQEIV 430
HL++ VHG+ + D V+ L+ L L + S TF D RL +E+
Sbjct: 102 HLLVLVHGILASPSDWLYVEAELKRRLGRRFLIYASSSNTFTKTFGGIDGAGKRLAEEVR 161
Query: 431 THIQNSSEPARISFVGHSLGTIIIRSALA 517
+Q S +ISF+ HSLG + R A+A
Sbjct: 162 QVVQKSKSLKKISFLAHSLGGLFSRHAVA 190
>At5g51180.2 68418.m06346 expressed protein
Length = 357
Score = 48.8 bits (111), Expect = 2e-06
Identities = 29/91 (31%), Positives = 46/91 (50%), Gaps = 1/91 (1%)
Frame = +2
Query: 251 HLIICVHGLDGNSGDLRLVKTYLELGLPGARLDFLMSERNQGD-TFSDFDTMTDRLVQEI 427
HL++ VHG+ G++ D + +P ++ SE+N T D M +RL E+
Sbjct: 35 HLVVMVHGILGSTDDWKFGAEQFVKKMPD-KVFVHCSEKNVSALTLDGVDVMGERLAAEV 93
Query: 428 VTHIQNSSEPARISFVGHSLGTIIIRSALAR 520
+ IQ +ISFV HSLG + R A+ +
Sbjct: 94 LDIIQRKPNICKISFVAHSLGGLAARYAIGK 124
>At5g51180.1 68418.m06345 expressed protein
Length = 357
Score = 48.8 bits (111), Expect = 2e-06
Identities = 29/91 (31%), Positives = 46/91 (50%), Gaps = 1/91 (1%)
Frame = +2
Query: 251 HLIICVHGLDGNSGDLRLVKTYLELGLPGARLDFLMSERNQGD-TFSDFDTMTDRLVQEI 427
HL++ VHG+ G++ D + +P ++ SE+N T D M +RL E+
Sbjct: 35 HLVVMVHGILGSTDDWKFGAEQFVKKMPD-KVFVHCSEKNVSALTLDGVDVMGERLAAEV 93
Query: 428 VTHIQNSSEPARISFVGHSLGTIIIRSALAR 520
+ IQ +ISFV HSLG + R A+ +
Sbjct: 94 LDIIQRKPNICKISFVAHSLGGLAARYAIGK 124
>At1g73750.1 68414.m08539 expressed protein contains Pfam profile:
PF00561 alpha/beta hydrolase fold
Length = 452
Score = 29.9 bits (64), Expect = 1.2
Identities = 15/50 (30%), Positives = 27/50 (54%), Gaps = 2/50 (4%)
Frame = +2
Query: 389 DFDTMTDRLVQEIVTHI--QNSSEPARISFVGHSLGTIIIRSALARAQMK 532
DFD + V + ++ Q S+ ++ VGHS+G I++ + L+R K
Sbjct: 204 DFDNYLEEDVPSAMDYVRTQTKSKDGKLLAVGHSMGGILLYALLSRCGFK 253
>At1g63990.1 68414.m07248 DNA topoisomerase VIA, putative (SPO11-2)
similar to topoisomerase 6 subunit A (spo11)
[Arabidopsis thaliana] GI:12331186; contains Pfam
profile PF04406: Type IIB DNA topoisomerase; identical
to cDNA putative topoisomerase VIA (SPO11 gene 2)
GI:7270976
Length = 383
Score = 29.9 bits (64), Expect = 1.2
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = -2
Query: 309 FTRRKSPEFPSNPWTQIIRWSPSGLNILYSSLIWKYGS 196
F R S FP P ++ W+P+GL IL + +K+GS
Sbjct: 252 FLHRMSTTFPDLPILVLVDWNPAGLAILCT---FKFGS 286
>At3g46930.1 68416.m05093 protein kinase family protein contains
protein kinase domain, Pfam:PF00069
Length = 475
Score = 29.5 bits (63), Expect = 1.6
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 3/50 (6%)
Frame = +2
Query: 410 RLVQEIV---THIQNSSEPARISFVGHSLGTIIIRSALARAQMKAYLGKL 550
RL +E + T + S P + FVG + G II + R +++YL KL
Sbjct: 204 RLEKEFIVEATLLSRLSHPNVVKFVGVNTGNCIITEYVPRGSLRSYLHKL 253
>At1g18620.1 68414.m02321 expressed protein
Length = 978
Score = 29.5 bits (63), Expect = 1.6
Identities = 28/96 (29%), Positives = 45/96 (46%), Gaps = 1/96 (1%)
Frame = +2
Query: 35 KILQKSENKRESNLISIFGLAESERLFVKCREEFRQSVKYPGAIYSDFPPVDNSLPYFHI 214
+ILQK KR NL+ + E E F+KC + + + ++DF D+++P +
Sbjct: 888 EILQKQAKKRSENLLLLEEEEEEEEDFLKCILD--EDMAIQSEKWTDF---DDAIPGLVL 942
Query: 215 SDEYRMFNPEGLHLIICVHG-LDGNSGDLRLVKTYL 319
E +F + VHG +D G+ R KT L
Sbjct: 943 DMERLLFKDLVKEI---VHGEIDRLQGNSRRQKTVL 975
>At1g02660.1 68414.m00216 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 713
Score = 29.5 bits (63), Expect = 1.6
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +2
Query: 401 MTDRLVQEIVTHIQNSSEPARISFVGHSLG 490
M ++++ E+ HI+ A+ F GHSLG
Sbjct: 401 MYEQMLPEVKAHIKTHGTSAKFRFTGHSLG 430
>At1g15060.1 68414.m01800 expressed protein
Length = 578
Score = 28.7 bits (61), Expect = 2.9
Identities = 13/46 (28%), Positives = 27/46 (58%), Gaps = 2/46 (4%)
Frame = +2
Query: 389 DFDTMTDRLVQEIVTHIQNSSEP--ARISFVGHSLGTIIIRSALAR 520
DFD + V + +++ S+P ++ +GHS+G I++ + L+R
Sbjct: 330 DFDHYLEEDVPAAIEYVRAQSKPKDGKLFAIGHSMGGILLYAMLSR 375
>At3g61680.1 68416.m06912 lipase class 3 family protein contains
Pfam profile PF01764: Lipase
Length = 649
Score = 28.3 bits (60), Expect = 3.8
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = +2
Query: 407 DRLVQEIVTHIQNSSEPARISFVGHSLG 490
++ + EI H+ + A+ F GHSLG
Sbjct: 397 EQFLPEITEHLSRHGDRAKFQFTGHSLG 424
>At3g20440.1 68416.m02588 glycoside hydrolase family 13 protein
similar to 1,4-alpha-glucan branching enzyme [Solanum
tuberosum] GI:1621012, 1,4-alpha-glucan branching enzyme
(EC 2.4.1.18) from [Homo sapiens] SP|Q04446, {Solanum
tuberosum} SP|P30924; contains Pfam profiles: PF00128
Alpha amylase catalytic domain, PF02922 Isoamylase
N-terminal domain
Length = 777
Score = 28.3 bits (60), Expect = 3.8
Identities = 20/61 (32%), Positives = 25/61 (40%), Gaps = 4/61 (6%)
Frame = +1
Query: 373 RRHFLRFRHYDR*TCTRNCHAHSK----FKRTGPNKLRGALFGDNNNQIRSSKGPNEGLF 540
+RHF FR + H H + F GP GA+ GD N + EGLF
Sbjct: 105 KRHF-DFRDFASGFELLGMHRHMEHRVDFMDWGPGSRYGAIIGDFNGWSPTENAAREGLF 163
Query: 541 G 543
G
Sbjct: 164 G 164
>At1g65440.1 68414.m07424 glycine-rich protein
Length = 1647
Score = 27.9 bits (59), Expect = 5.0
Identities = 10/22 (45%), Positives = 15/22 (68%)
Frame = +2
Query: 377 DTFSDFDTMTDRLVQEIVTHIQ 442
DTF D D + DR V +V+H++
Sbjct: 1301 DTFEDLDEVMDRYVDPLVSHLK 1322
>At3g52900.1 68416.m05830 expressed protein contains Pfam profile
PF04949: Family of unknown function (DUF662)
Length = 164
Score = 27.5 bits (58), Expect = 6.6
Identities = 15/34 (44%), Positives = 24/34 (70%), Gaps = 2/34 (5%)
Frame = +2
Query: 53 ENKRESNLISIFG--LAESERLFVKCREEFRQSV 148
+NK +++L+S+ LAESERL +K EE ++V
Sbjct: 127 KNKEKAHLVSMLMELLAESERLRIKKLEEINKTV 160
>At2g44810.1 68415.m05576 defective in anther dehiscence1 (DAD1)
identical to DEFECTIVE IN ANTHER DEHISCENCE1
[Arabidopsis thaliana] GI:16215706; contains Pfam
profile PF01764: Lipase
Length = 357
Score = 27.5 bits (58), Expect = 6.6
Identities = 23/75 (30%), Positives = 34/75 (45%), Gaps = 6/75 (8%)
Frame = +2
Query: 293 DLRLVKTYLELGLPGARLDFLMSERNQGDTF-----SDFDTMTDRLVQEIVTHIQN-SSE 454
+LR T+L G GA L+ S F S ++ D + +EI +Q+ E
Sbjct: 136 NLRATLTHLPNGPTGANLNGSNSGPMVESGFLSLYTSGVHSLRDMVREEIARLLQSYGDE 195
Query: 455 PARISFVGHSLGTII 499
P ++ GHSLG I
Sbjct: 196 PLSVTITGHSLGAAI 210
>At1g63210.1 68414.m07144 hypothetical protein contains Pfam profile:
PF04283 protein of unknown function (DUF439)
Length = 1197
Score = 27.5 bits (58), Expect = 6.6
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 377 DTFSDFDTMTDRLVQEIVTHI 439
+TF D D + DR V +VTH+
Sbjct: 1085 ETFEDLDEVMDRYVDPLVTHL 1105
>At1g26680.1 68414.m03250 transcriptional factor B3 family protein
low similarity to reproductive meristem gene 1 from
[Brassica oleracea var. botrytis] GI:3170424,
[Arabidopsis thaliana] GI:13604227; contains Pfam
profile PF02362: B3 DNA binding domain
Length = 920
Score = 27.5 bits (58), Expect = 6.6
Identities = 9/26 (34%), Positives = 14/26 (53%)
Frame = -2
Query: 216 LIWKYGSELSTGGKSEYIAPGYFTLC 139
L WK+ + + GK Y+ PG+ C
Sbjct: 659 LSWKFNLKYNQAGKHTYVRPGWSRFC 684
>At5g01970.1 68418.m00116 expressed protein
Length = 351
Score = 27.1 bits (57), Expect = 8.7
Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = +2
Query: 365 RNQG-DTFSDFDTMTDRLVQEIVTHIQNSSEPARIS 469
R QG F+ F DRL E +T +++ S PAR S
Sbjct: 5 RRQGIGKFATFKEEVDRLPPESITAVKDRSPPARSS 40
>At1g09530.2 68414.m01069 phytochrome interacting factor 3 (PIF3)
identical to phytochrome interacting factor 3 (PIF3)
GI:3929585 from [Arabidopsis thaliana]
Length = 524
Score = 27.1 bits (57), Expect = 8.7
Identities = 12/49 (24%), Positives = 20/49 (40%)
Frame = -2
Query: 174 SEYIAPGYFTLCLNSSLHFTNSRSDSASPNXXXXXXXXXXXXFCNILND 28
S ++ P F N++LH T +S + PN +LN+
Sbjct: 201 SHFLRPATFAKTTNNNLHDTKEKSPQSPPNVFQTRVLGAKDSEDKVLNE 249
>At1g09530.1 68414.m01068 phytochrome interacting factor 3 (PIF3)
identical to phytochrome interacting factor 3 (PIF3)
GI:3929585 from [Arabidopsis thaliana]
Length = 524
Score = 27.1 bits (57), Expect = 8.7
Identities = 12/49 (24%), Positives = 20/49 (40%)
Frame = -2
Query: 174 SEYIAPGYFTLCLNSSLHFTNSRSDSASPNXXXXXXXXXXXXFCNILND 28
S ++ P F N++LH T +S + PN +LN+
Sbjct: 201 SHFLRPATFAKTTNNNLHDTKEKSPQSPPNVFQTRVLGAKDSEDKVLNE 249
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,337,766
Number of Sequences: 28952
Number of extensions: 266727
Number of successful extensions: 758
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 741
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 752
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1092379416
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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