Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_H13
(563 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 225 3e-60
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 213 2e-56
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 27 1.9
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 1.9
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 26 3.3
SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyce... 25 5.8
SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 25 5.8
SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase |Schiz... 25 5.8
SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|c... 25 7.7
SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces pomb... 25 7.7
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 25 7.7
SPBP35G2.04c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 25 7.7
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 225 bits (551), Expect = 3e-60
Identities = 105/147 (71%), Positives = 124/147 (84%)
Frame = +1
Query: 1 EKELREICYDVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQ 180
E+EL IC D+L +L+KHLIP A++ ESKVFY KMKGDYYRYLAE A GE R + S
Sbjct: 92 EQELDTICQDILTVLEKHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSADQSL 151
Query: 181 KAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTL 360
+ Y+ A EI+ A++ PTHPIRLGLALNFSVFYYEILNSPD+AC LAKQAFD+AI+ELD+L
Sbjct: 152 EGYKAASEIATAELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSL 211
Query: 361 NEDSYKDSTLIMQLLRDNLTLWTSDTQ 441
+E+SYKDSTLIMQLLRDNLTLWTSD +
Sbjct: 212 SEESYKDSTLIMQLLRDNLTLWTSDAE 238
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 213 bits (519), Expect = 2e-56
Identities = 99/147 (67%), Positives = 120/147 (81%)
Frame = +1
Query: 1 EKELREICYDVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQ 180
E EL +IC+DVL +L+KHLIP A+ ESKVFY KMKGDYYRYLAE GE + S
Sbjct: 91 EDELSDICHDVLSVLEKHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADSSL 150
Query: 181 KAYQDAFEISKAKMQPTHPIRLGLALNFSVFYYEILNSPDKACQLAKQAFDDAIAELDTL 360
+AY+ A +I+ A++ PT P+RLGLALNFSVFYYEIL+SP+ AC LAKQ FD+AI+ELD+L
Sbjct: 151 EAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISELDSL 210
Query: 361 NEDSYKDSTLIMQLLRDNLTLWTSDTQ 441
+E+SYKDSTLIMQLLRDNLTLWTSD +
Sbjct: 211 SEESYKDSTLIMQLLRDNLTLWTSDAE 237
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 27.1 bits (57), Expect = 1.9
Identities = 17/51 (33%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Frame = +1
Query: 121 RYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAKMQPTHP-IRLGLALNFS 267
RY+ ++ E R ED+++A + ++ + A +QP H I L LA+N S
Sbjct: 1049 RYIVALSCELERRRLGEEDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 1.9
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Frame = +1
Query: 40 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 177
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 26.2 bits (55), Expect = 3.3
Identities = 20/81 (24%), Positives = 34/81 (41%), Gaps = 2/81 (2%)
Frame = +1
Query: 178 QKAYQDAFEISKAKMQPTHPIRL-GLALNF-SVFYYEILNSPDKACQLAKQAFDDAIAEL 351
Q+ D E + P H L F S Y ++ D QLA Q F ++
Sbjct: 415 QRLVADLHEFYNSVKGPAHSFETEDPVLKFVSQSYDDLFPYMDNFIQLAVQLFYHISKKV 474
Query: 352 DTLNEDSYKDSTLIMQLLRDN 414
+ L +++ S ++Q ++DN
Sbjct: 475 NCLYVEAFSSSDAVLQRIQDN 495
>SPAC3G6.11 |||ATP-dependent DNA helicase Chl1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 844
Score = 25.4 bits (53), Expect = 5.8
Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 3/36 (8%)
Frame = +1
Query: 31 VLCLLDKHLIPKASN-PESKVFYLKMKGD--YYRYL 129
VL L+ L+ A+ PE K+FY K GD Y +YL
Sbjct: 509 VLMQLESFLLNIANPAPEGKLFYEKQTGDNPYLKYL 544
>SPAC30D11.02c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 85
Score = 25.4 bits (53), Expect = 5.8
Identities = 13/34 (38%), Positives = 19/34 (55%)
Frame = -3
Query: 231 CGLHFCLADLKSILVCFL*ILYNRMSGFSCGHFC 130
C L+F L + K+ L+ I+Y + GFS H C
Sbjct: 46 CLLNFSLRENKNYLI----IVYLPIEGFSANHMC 75
>SPAC21E11.08 |lcb2|SPAC2C4.02|serine palmitoyltransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 603
Score = 25.4 bits (53), Expect = 5.8
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -3
Query: 141 GHFCEIPVVISLHFEVKYFTF 79
G+FC++P V+ L K++ F
Sbjct: 341 GNFCDLPKVVELKNRYKFYLF 361
>SPBC1683.05 |||thiamine transporter |Schizosaccharomyces pombe|chr
2|||Manual
Length = 559
Score = 25.0 bits (52), Expect = 7.7
Identities = 15/64 (23%), Positives = 29/64 (45%), Gaps = 1/64 (1%)
Frame = -3
Query: 237 WVCGLHFCLADLKSILVCFL*ILYNRMSGF-SCGHFCEIPVVISLHFEVKYFTFWITSFR 61
++ GL + ++ + C ILY F S + I+ F + YF +W+ SF
Sbjct: 152 FIAGLFYYGTNVYTGAECVQTILYAIFKSFRSYKNRLPADAGITSDFLISYFVYWVISFP 211
Query: 60 YKVL 49
+ ++
Sbjct: 212 FHLI 215
>SPAC664.02c |||actin-like protein Arp8 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 620
Score = 25.0 bits (52), Expect = 7.7
Identities = 21/83 (25%), Positives = 33/83 (39%)
Frame = +1
Query: 28 DVLCLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEI 207
DV+C + L+P PE K+FY + +G S SQ+ D +EI
Sbjct: 178 DVICGKEAFLLPLNEYPEYKLFY------------PIKSGVFNESDYASSQQLLADIYEI 225
Query: 208 SKAKMQPTHPIRLGLALNFSVFY 276
K + I + +SV +
Sbjct: 226 FKYSITSLLQIPVSQLSQYSVIF 248
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 25.0 bits (52), Expect = 7.7
Identities = 13/42 (30%), Positives = 18/42 (42%)
Frame = +1
Query: 52 HLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDS 177
H SNP S K KG++ Y A G ++ +DS
Sbjct: 225 HTSKTYSNPSSFATRKKEKGEHLSYAEAAAVGTQAKNIKKDS 266
>SPBP35G2.04c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 249
Score = 25.0 bits (52), Expect = 7.7
Identities = 10/20 (50%), Positives = 14/20 (70%), Gaps = 1/20 (5%)
Frame = -3
Query: 126 IPVVISLHFEVKYFT-FWIT 70
+P++I LHF K FT W+T
Sbjct: 62 LPILIRLHFYKKLFTILWLT 81
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,048,205
Number of Sequences: 5004
Number of extensions: 38723
Number of successful extensions: 113
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 113
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 113
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 238029836
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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