Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_G12
(624 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g06850.2 68416.m00813 branched chain alpha-keto acid dehydrog... 73 2e-13
At3g06850.1 68416.m00812 branched chain alpha-keto acid dehydrog... 73 2e-13
At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase, p... 39 0.002
At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein ... 38 0.007
At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase, p... 35 0.038
At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase, p... 34 0.067
At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase, p... 34 0.088
At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase (L... 33 0.15
At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein ... 32 0.36
At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein ... 32 0.36
At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP... 31 0.47
At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein ... 31 0.47
At4g00450.1 68417.m00062 expressed protein 30 1.1
At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP... 29 1.9
At2g43230.1 68415.m05373 serine/threonine protein kinase, putati... 29 3.3
At2g27240.1 68415.m03273 expressed protein contains Pfam profile... 28 4.4
At3g09960.1 68416.m01194 calcineurin-like phosphoesterase family... 28 5.8
>At3g06850.2 68416.m00813 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 72.5 bits (170), Expect = 2e-13
Identities = 33/77 (42%), Positives = 47/77 (61%)
Frame = +2
Query: 392 IVAFKLSDIGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITRLYH 571
++ L+ GEGI E + +WFVK GD+VE+F +CEVQSDKA + ITSR+ G + + H
Sbjct: 75 LIDVPLAQTGEGIAECELLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISH 134
Query: 572 EVDQTALVGNPLIDIDV 622
VG L+ + V
Sbjct: 135 SPGDIIKVGETLVRLAV 151
>At3g06850.1 68416.m00812 branched chain alpha-keto acid
dehydrogenase E2 subunit (din3) identical to branched
chain alpha-keto acid dehydrogenase E2 subunit (din3)
[Arabidopsis thaliana] GI:7021284
Length = 483
Score = 72.5 bits (170), Expect = 2e-13
Identities = 33/77 (42%), Positives = 47/77 (61%)
Frame = +2
Query: 392 IVAFKLSDIGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITRLYH 571
++ L+ GEGI E + +WFVK GD+VE+F +CEVQSDKA + ITSR+ G + + H
Sbjct: 75 LIDVPLAQTGEGIAECELLKWFVKEGDSVEEFQPLCEVQSDKATIEITSRFKGKVALISH 134
Query: 572 EVDQTALVGNPLIDIDV 622
VG L+ + V
Sbjct: 135 SPGDIIKVGETLVRLAV 151
>At3g52200.1 68416.m05733 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide acetyltransferase
(E2) subunit of PDC [Arabidopsis thaliana] GI:559395;
contains Pfam profiles PF00198: 2-oxo acid
dehydrogenases acyltransferase (catalytic domain),
PF00364: Biotin-requiring enzyme, PF02817: e3 binding
domain; supporting cDNA
gi|5881964|gb|AF066080.1|AF066080
Length = 637
Score = 39.1 bits (87), Expect = 0.002
Identities = 15/41 (36%), Positives = 26/41 (63%)
Frame = +2
Query: 443 IKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITRL 565
+ +W K GD VE D +CE+++DKA V S+ +G + ++
Sbjct: 102 VVKWMKKEGDKVEVGDVLCEIETDKATVEFESQEEGFLAKI 142
Score = 36.7 bits (81), Expect = 0.013
Identities = 19/56 (33%), Positives = 31/56 (55%), Gaps = 1/56 (1%)
Frame = +2
Query: 443 IKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITR-LYHEVDQTALVGNPL 607
I +W+ K GD +E D I E+++DKA + S +G + + L E + VG P+
Sbjct: 229 IAKWWKKEGDKIEVGDVIGEIETDKATLEFESLEEGYLAKILIPEGSKDVAVGKPI 284
>At5g55070.1 68418.m06864 2-oxoacid dehydrogenase family protein
similar to SP|Q01205 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Rattus norvegicus}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 37.5 bits (83), Expect = 0.007
Identities = 24/88 (27%), Positives = 40/88 (45%)
Frame = +2
Query: 353 RWRRFHTNQILNKIVAFKLSDIGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKAAVTI 532
RW R ++ +V + +GE I + + + K GD VE + I ++++DK + I
Sbjct: 81 RWVRPFSSDS-GDVVEAVVPHMGESITDGTLAAFLKKPGDRVEADEAIAQIETDKVTIDI 139
Query: 533 TSRYDGVITRLYHEVDQTALVGNPLIDI 616
S GVI + T GN + I
Sbjct: 140 ASPASGVIQEFLVKEGDTVEPGNKVARI 167
>At3g13930.1 68416.m01759 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
[Zea mays] GI:5669871; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 35.1 bits (77), Expect = 0.038
Identities = 15/44 (34%), Positives = 24/44 (54%)
Frame = +2
Query: 443 IKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITRLYHE 574
I W K GD V + +CEV++DKA V + +G + ++ E
Sbjct: 128 IARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGFLAKIVKE 171
>At1g34430.1 68414.m04277 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963; contains Pfam
profiles PF00198: 2-oxo acid dehydrogenases
acyltransferase (catalytic domain), PF00364:
Biotin-requiring enzyme, PF02817: e3 binding domain
Length = 465
Score = 34.3 bits (75), Expect = 0.067
Identities = 19/73 (26%), Positives = 35/73 (47%)
Frame = +2
Query: 389 KIVAFKLSDIGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITRLY 568
KI + + + E I W GD + + +++ V+SDKA + + + YDG + +
Sbjct: 38 KIREIFMPALSSTMTEGKIVSWVKSEGDKLNKGESVVVVESDKADMDVETFYDGYLAAIM 97
Query: 569 HEVDQTALVGNPL 607
E A VG+ +
Sbjct: 98 VEEGGVAPVGSAI 110
>At1g54220.1 68414.m06182 dihydrolipoamide S-acetyltransferase,
putative similar to dihydrolipoamide S-acetyltransferase
GI:5669871 [Zea mays]; contains Pfam profiles PF00198:
2-oxo acid dehydrogenases acyltransferase (catalytic
domain), PF00364: Biotin-requiring enzyme, PF02817: e3
binding domain
Length = 539
Score = 33.9 bits (74), Expect = 0.088
Identities = 14/41 (34%), Positives = 23/41 (56%)
Frame = +2
Query: 443 IKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITRL 565
I W K GD V + +CEV++DKA V + +G + ++
Sbjct: 128 IARWLKKEGDKVAPGEVLCEVETDKATVEMECMEEGYLAKI 168
>At3g25860.1 68416.m03222 dihydrolipoamide S-acetyltransferase
(LTA2) identical to dihydrolipoamide S-acetyltransferase
(LTA2) [Arabidopsis thaliana] GI:5881963
Length = 480
Score = 33.1 bits (72), Expect = 0.15
Identities = 18/73 (24%), Positives = 36/73 (49%)
Frame = +2
Query: 380 ILNKIVAFKLSDIGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVIT 559
+ +KI + + + E I W G+ + + +++ V+SDKA + + + YDG +
Sbjct: 51 VRSKIREIFMPALSSTMTEGKIVSWIKTEGEKLAKGESVVVVESDKADMDVETFYDGYLA 110
Query: 560 RLYHEVDQTALVG 598
+ +TA VG
Sbjct: 111 AIVVGEGETAPVG 123
>At4g26910.2 68417.m03873 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 463
Score = 31.9 bits (69), Expect = 0.36
Identities = 18/68 (26%), Positives = 35/68 (51%)
Frame = +2
Query: 353 RWRRFHTNQILNKIVAFKLSDIGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKAAVTI 532
RW R + + + + A + +GE I + + + K G+ V+ + I ++++DK + I
Sbjct: 79 RWVRPFSAETGDTVEAV-VPHMGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDI 137
Query: 533 TSRYDGVI 556
S GVI
Sbjct: 138 ASPASGVI 145
>At4g26910.1 68417.m03872 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 464
Score = 31.9 bits (69), Expect = 0.36
Identities = 18/68 (26%), Positives = 35/68 (51%)
Frame = +2
Query: 353 RWRRFHTNQILNKIVAFKLSDIGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKAAVTI 532
RW R + + + + A + +GE I + + + K G+ V+ + I ++++DK + I
Sbjct: 80 RWVRPFSAETGDTVEAV-VPHMGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDI 138
Query: 533 TSRYDGVI 556
S GVI
Sbjct: 139 ASPASGVI 146
>At5g15530.1 68418.m01818 biotin carboxyl carrier protein 2 (BCCP2)
identical to biotin carboxyl carrier protein isoform 2
[Arabidopsis thaliana] gi|8886869|gb|AAF80592
Length = 255
Score = 31.5 bits (68), Expect = 0.47
Identities = 18/54 (33%), Positives = 26/54 (48%)
Frame = +2
Query: 455 FVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITRLYHEVDQTALVGNPLIDI 616
FVKVGD V++ +C +++ K I + G I L E + V PL I
Sbjct: 200 FVKVGDKVQKGQIVCIIEAMKLMNEIEAEKSGTIMELLAEDGKPVSVDTPLFVI 253
>At4g26910.3 68417.m03871 2-oxoacid dehydrogenase family protein
similar to SP|P36957 Dihydrolipoamide
succinyltransferase component of 2-oxoglutarate
dehydrogenase complex, mitochondrial precursor (EC
2.3.1.61) {Homo sapiens}; contains Pfam profiles
PF00198: 2-oxo acid dehydrogenases acyltransferase
(catalytic domain), PF00364: Biotin-requiring enzyme
Length = 365
Score = 31.5 bits (68), Expect = 0.47
Identities = 14/47 (29%), Positives = 26/47 (55%)
Frame = +2
Query: 416 IGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVI 556
+GE I + + + K G+ V+ + I ++++DK + I S GVI
Sbjct: 1 MGESITDGTLATFLKKPGERVQADEAIAQIETDKVTIDIASPASGVI 47
>At4g00450.1 68417.m00062 expressed protein
Length = 2124
Score = 30.3 bits (65), Expect = 1.1
Identities = 19/49 (38%), Positives = 29/49 (59%), Gaps = 3/49 (6%)
Frame = -1
Query: 471 SPTLTNHSLI-TTSRIPSPISDSLKATILLRI*FVWKRLHLIC--PSFE 334
SP+L S TT P P +L+A++ LR+ F+ + L +IC PSF+
Sbjct: 1846 SPSLARRSSANTTDTSPPPSPAALRASMSLRLQFLLRLLPVICGEPSFK 1894
>At5g16390.1 68418.m01915 biotin carboxyl carrier protein 1 (BCCP1)
identical to biotin carboxyl carrier protein of
acetyl-CoA carboxylase precursor [Arabidopsis thaliana]
gi|9759121|dbj|BAB09606
Length = 280
Score = 29.5 bits (63), Expect = 1.9
Identities = 15/51 (29%), Positives = 26/51 (50%)
Frame = +2
Query: 455 FVKVGDTVEQFDNICEVQSDKAAVTITSRYDGVITRLYHEVDQTALVGNPL 607
F+KVGD V++ +C V++ K I S + G + + E + + PL
Sbjct: 225 FIKVGDKVQKGQVLCIVEAMKLMNEIESDHTGTVVDIVAEDGKPVSLDTPL 275
>At2g43230.1 68415.m05373 serine/threonine protein kinase, putative
similar to Pto kinase interactor 1 (Pti1)[Lycopersicon
esculentum] gi|3668069|gb|AAC61805
Length = 406
Score = 28.7 bits (61), Expect = 3.3
Identities = 14/32 (43%), Positives = 20/32 (62%), Gaps = 1/32 (3%)
Frame = -3
Query: 457 EPFFNHDFSDSFANIRQLKSDNFIENL-ICVE 365
EP N +F + + +LKSDNF++ L CVE
Sbjct: 146 EPETNVEFLTQVSKVSRLKSDNFVQLLGYCVE 177
>At2g27240.1 68415.m03273 expressed protein contains Pfam profile
PF01027: Uncharacterized protein family UPF0005
Length = 506
Score = 28.3 bits (60), Expect = 4.4
Identities = 15/47 (31%), Positives = 26/47 (55%)
Frame = +2
Query: 380 ILNKIVAFKLSDIGEGIREVVIKEWFVKVGDTVEQFDNICEVQSDKA 520
+L KI +++L+D G+ EVV + +V + FDN V + K+
Sbjct: 221 LLRKIKSWRLADFGDKYCEVVENDGAKEVDKRKKDFDNYKSVLNSKS 267
>At3g09960.1 68416.m01194 calcineurin-like phosphoesterase family
protein contains Pfam profile: PF00149 calcineurin-like
phosphoesterase
Length = 311
Score = 27.9 bits (59), Expect = 5.8
Identities = 10/27 (37%), Positives = 18/27 (66%)
Frame = +2
Query: 245 NAGRAHKVWLLMQSDRHRSSFTSNIIL 325
N + +K+WL +QSD S F+S +++
Sbjct: 18 NISKLNKLWLNLQSDIQNSDFSSALVI 44
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,947,947
Number of Sequences: 28952
Number of extensions: 221374
Number of successful extensions: 619
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 610
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 619
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1265787216
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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