Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_G04
         (603 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC1450.04 |tef5||translation elongation factor EF-1 beta subun...    90   3e-19
SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces po...    27   2.1  
SPAC24C9.12c |||glycine hydroxymethyltransferase |Schizosaccharo...    26   4.9  
SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces...    25   6.4  
SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein L7|...    25   6.4  

>SPCC1450.04 |tef5||translation elongation factor EF-1 beta subunit
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 214

 Score = 89.8 bits (213), Expect = 3e-19
 Identities = 39/63 (61%), Positives = 47/63 (74%)
 Frame = +3

Query: 414 YADKKSKKPALIAKSSXILDVXPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYGIN 593
           Y  KK+ KP  + KS   LDV PWDDET M E+E  VR+I+MDGL+WG SKLVPVG+G+N
Sbjct: 115 YNKKKAAKPKAVHKSLVTLDVKPWDDETPMDELEKAVRSIQMDGLVWGLSKLVPVGFGVN 174

Query: 594 KLQ 602
           K Q
Sbjct: 175 KFQ 177



 Score = 62.9 bits (146), Expect = 3e-11
 Identities = 28/63 (44%), Positives = 39/63 (61%), Gaps = 1/63 (1%)
 Frame = +2

Query: 47  MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVP-AASLPHVLRWYSHI 223
           M   D+ +  GL +LN +L ++S++ GY PSQAD  VF+ VG  P  A  P+  RWY  I
Sbjct: 1   MGFSDLTSDAGLKQLNDFLLDKSFIEGYEPSQADAVVFKAVGVAPDTAKYPNGARWYKQI 60

Query: 224 ASY 232
           A+Y
Sbjct: 61  ATY 63


>SPAC323.01c |||mitochondrial NADH kinase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 361

 Score = 27.1 bits (57), Expect = 2.1
 Identities = 17/77 (22%), Positives = 39/77 (50%), Gaps = 5/77 (6%)
 Frame = +3

Query: 354 LVMRKRMQKQPES-----VKKVLRHYADKKSKKPALIAKSSXILDVXPWDDETDMKEMEN 518
           L+++KRM ++ +      V+ + + Y D        +AK    L++  W + +D+++  +
Sbjct: 67  LILKKRMDERVDHCFETLVQHLQQTYPDICIITETDVAKKFSYLNLYTWTEISDLEQKVD 126

Query: 519 QVRTIEMDGLLWGASKL 569
            + T+  DG +  A+ L
Sbjct: 127 AIITVGGDGTILHAASL 143


>SPAC24C9.12c |||glycine hydroxymethyltransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 25.8 bits (54), Expect = 4.9
 Identities = 10/35 (28%), Positives = 21/35 (60%)
 Frame = +3

Query: 357 VMRKRMQKQPESVKKVLRHYADKKSKKPALIAKSS 461
           +M   +++Q  +V +++RH AD++     LIA  +
Sbjct: 7   IMLTPLKEQDPTVAEIMRHEADRQRSSVVLIASEN 41


>SPBC1198.04c |zas1||zinc finger protein Zas1|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 897

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 12/34 (35%), Positives = 19/34 (55%)
 Frame = +3

Query: 423 KKSKKPALIAKSSXILDVXPWDDETDMKEMENQV 524
           +KSK P ++      LDV PWD   + K++  Q+
Sbjct: 858 EKSKNPIILHVVLQALDVFPWDLLGEHKKIIQQL 891


>SPAC3H5.07 |rpl702|rpl7-2, rpl7, rpl7b|60S ribosomal protein
           L7|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 250

 Score = 25.4 bits (53), Expect = 6.4
 Identities = 19/66 (28%), Positives = 33/66 (50%), Gaps = 3/66 (4%)
 Frame = +3

Query: 339 SIFLALVMRKRMQKQPESVKKVLRHYADKKS---KKPALIAKSSXILDVXPWDDETDMKE 509
           SIF    + K+ + Q +S ++++   A+KKS   KK  LIAK +   +      E +  E
Sbjct: 12  SIFAPESLLKKTKAQKQSREQIVAAAAEKKSARQKKRELIAKRAEAYEAEYRAAEREQIE 71

Query: 510 MENQVR 527
           +  + R
Sbjct: 72  LARKAR 77


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,123,543
Number of Sequences: 5004
Number of extensions: 35555
Number of successful extensions: 78
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 75
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 77
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 264253462
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)