Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_G04
(603 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2 (e... 93 2e-19
At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1 (e... 88 4e-18
At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta id... 88 4e-18
At2g18110.1 68415.m02105 elongation factor 1-beta, putative / EF... 87 6e-18
At2g40660.1 68415.m05017 tRNA-binding region domain-containing p... 32 0.25
At5g08415.1 68418.m00991 lipoic acid synthase family protein sim... 30 1.4
At4g18710.1 68417.m02766 shaggy-related protein kinase eta / ASK... 29 2.4
At1g06390.2 68414.m00676 shaggy-related protein kinase iota / AS... 29 3.1
At1g06390.1 68414.m00675 shaggy-related protein kinase iota / AS... 29 3.1
At2g36190.1 68415.m04442 beta-fructosidase, putative / beta-fruc... 28 4.1
At2g30980.1 68415.m03778 shaggy-related protein kinase delta / A... 28 5.5
At2g44460.1 68415.m05528 glycosyl hydrolase family 1 protein con... 27 7.2
At2g05170.1 68415.m00544 vacuolar protein sorting 11 family prot... 27 7.2
At5g01280.1 68418.m00037 expressed protein 27 9.6
>At5g19510.1 68418.m02324 elongation factor 1B alpha-subunit 2
(eEF1Balpha2) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686821
Length = 224
Score = 92.7 bits (220), Expect = 2e-19
Identities = 42/64 (65%), Positives = 48/64 (75%)
Frame = +3
Query: 408 RHYADKKSKKPALIAKSSXILDVXPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYG 587
R A K +KKP KSS ++DV PWDDETDMK++E VR +EM GL WGASKLVPVGYG
Sbjct: 120 REAAKKDTKKPKESGKSSVLMDVKPWDDETDMKKLEEAVRGVEMPGLFWGASKLVPVGYG 179
Query: 588 INKL 599
I KL
Sbjct: 180 IKKL 183
Score = 47.2 bits (107), Expect = 8e-06
Identities = 19/61 (31%), Positives = 36/61 (59%)
Frame = +2
Query: 47 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 226
+ D+ T +G+ + ++LA ++Y+SG S D+KV+ V P+ + P+ +WY +A
Sbjct: 3 VTFSDLHTEEGVKSVEEHLAGKTYISGDQLSVDDVKVYAAVPVKPSDAFPNASKWYESVA 62
Query: 227 S 229
S
Sbjct: 63 S 63
>At5g12110.1 68418.m01422 elongation factor 1B alpha-subunit 1
(eEF1Balpha1) identical to elongation factor 1B
alpha-subunit [Arabidopsis thaliana] GI:6686819
Length = 228
Score = 88.2 bits (209), Expect = 4e-18
Identities = 40/64 (62%), Positives = 48/64 (75%)
Frame = +3
Query: 408 RHYADKKSKKPALIAKSSXILDVXPWDDETDMKEMENQVRTIEMDGLLWGASKLVPVGYG 587
R A K +KK KSS +L+V PWDDETDMK++E VR+++M GL WGASKLVPVGYG
Sbjct: 124 REAAKKDTKKTKESGKSSVLLEVKPWDDETDMKKLEEAVRSVQMPGLTWGASKLVPVGYG 183
Query: 588 INKL 599
I KL
Sbjct: 184 IKKL 187
Score = 49.2 bits (112), Expect = 2e-06
Identities = 23/74 (31%), Positives = 40/74 (54%)
Frame = +2
Query: 47 MALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHIA 226
+ D+ T +GL L ++LA ++Y+SG S D+KV+ V + P P+ +WY +A
Sbjct: 3 VTFSDLHTERGLKTLEEHLAGKTYISGDQLSVDDVKVYAAVLENPGDGFPNASKWYDSVA 62
Query: 227 SYTPAERKTWSEGV 268
S+ ++GV
Sbjct: 63 SHLAKSFPGKADGV 76
>At1g30230.1 68414.m03698 elongation factor 1-beta / EF-1-beta
identical to SP|P48006 Elongation factor 1-beta
(EF-1-beta) {Arabidopsis thaliana}
Length = 231
Score = 88.2 bits (209), Expect = 4e-18
Identities = 42/77 (54%), Positives = 53/77 (68%), Gaps = 2/77 (2%)
Frame = +3
Query: 378 KQPESVKKVLRHYAD--KKSKKPALIAKSSXILDVXPWDDETDMKEMENQVRTIEMDGLL 551
++ E KK A K S K KSS ++D+ PWDDETDMK++E V++I+M+GL
Sbjct: 115 EETEEEKKAAEERAASVKASTKKKESGKSSVLIDIKPWDDETDMKKLEEAVKSIQMEGLF 174
Query: 552 WGASKLVPVGYGINKLQ 602
WGASKLVPVGYGI KLQ
Sbjct: 175 WGASKLVPVGYGIKKLQ 191
Score = 52.0 bits (119), Expect = 3e-07
Identities = 22/58 (37%), Positives = 36/58 (62%)
Frame = +2
Query: 50 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHI 223
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + + RWY+HI
Sbjct: 3 AFPNLNSDAGLKKLDEHLLTRSYITGYQASKDDITVFAALAKPPTSQYVNASRWYNHI 60
>At2g18110.1 68415.m02105 elongation factor 1-beta, putative /
EF-1-beta, putative nearly identical to eEF-1beta
[Arabidopsis thaliana] GI:398606
Length = 231
Score = 87.4 bits (207), Expect = 6e-18
Identities = 42/76 (55%), Positives = 52/76 (68%), Gaps = 2/76 (2%)
Frame = +3
Query: 378 KQPESVKKVLRHYAD--KKSKKPALIAKSSXILDVXPWDDETDMKEMENQVRTIEMDGLL 551
++ E KK A K S K KSS ++D+ PWDDETDMK++E VR+I+M+GL
Sbjct: 115 EETEEEKKAAEERAASVKASTKKKESGKSSVLMDIKPWDDETDMKKLEEAVRSIQMEGLF 174
Query: 552 WGASKLVPVGYGINKL 599
WGASKLVPVGYGI KL
Sbjct: 175 WGASKLVPVGYGIKKL 190
Score = 53.6 bits (123), Expect = 1e-07
Identities = 22/58 (37%), Positives = 37/58 (63%)
Frame = +2
Query: 50 ALGDVKTAQGLNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASLPHVLRWYSHI 223
A ++ + GL +L+++L RSY++GY S+ DI VF + K P + +V RW++HI
Sbjct: 3 AFPNLNSGSGLKKLDEHLLTRSYITGYQASKDDITVFTALSKPPTSEFVNVSRWFNHI 60
>At2g40660.1 68415.m05017 tRNA-binding region domain-containing
protein similar to SP|Q12904 Multisynthetase complex
auxiliary component p43 [Contains: Endothelial-monocyte
activating polypeptide II (EMAP-II) (Small inducible
cytokine subfamily E member 1)] {Homo sapiens}; contains
Pfam profile PF01588: Putative tRNA binding domain
Length = 389
Score = 32.3 bits (70), Expect = 0.25
Identities = 22/57 (38%), Positives = 32/57 (56%), Gaps = 9/57 (15%)
Frame = +2
Query: 80 LNELNQYLAERSYV--SGYTPSQADIKVFEQV-GKVPAAS------LPHVLRWYSHI 223
L +LN LA +S + +G TPS AD+ VF + V S +PHV+RW ++I
Sbjct: 76 LEKLNLELATKSVLLGNGLTPSAADVAVFSALHSSVLGLSDSDKEKVPHVIRWVNYI 132
>At5g08415.1 68418.m00991 lipoic acid synthase family protein
similar to lipoic acid synthase from Arabidopsis
thaliana [gi:3928758], from Mus musculus [gi:14669826]
Pfam profile PF04055: radical SAM domain protein
Length = 394
Score = 29.9 bits (64), Expect = 1.4
Identities = 20/57 (35%), Positives = 30/57 (52%)
Frame = +3
Query: 387 ESVKKVLRHYADKKSKKPALIAKSSXILDVXPWDDETDMKEMENQVRTIEMDGLLWG 557
E VL+H K KP +I K+S +L + D+E +KE +R I++D L G
Sbjct: 277 EQSMSVLKH---AKISKPGMITKTSIMLGLGETDEE--LKEAMADLRAIDVDILTLG 328
>At4g18710.1 68417.m02766 shaggy-related protein kinase eta /
ASK-eta (ASK7) identical to shaggy-related protein
kinase eta (ASK-eta) [Arabidopsis thaliana]
SWISS-PROT:Q39011
Length = 380
Score = 29.1 bits (62), Expect = 2.4
Identities = 16/51 (31%), Positives = 24/51 (47%)
Frame = +3
Query: 303 LLHXXXXXXXXXSIFLALVMRKRMQKQPESVKKVLRHYADKKSKKPALIAK 455
L H +FL LVM + PES+ +VL+HY+ + P + K
Sbjct: 96 LKHCFFSTTSKDELFLNLVM----EYVPESLYRVLKHYSSANQRMPLVYVK 142
>At1g06390.2 68414.m00676 shaggy-related protein kinase iota /
ASK-iota (ASK9) (GSK1) identical to shaggy-related
protein kinase iota (ASK-iota) [Arabidopsis thaliana]
SWISS-PROT:Q39012
Length = 407
Score = 28.7 bits (61), Expect = 3.1
Identities = 16/51 (31%), Positives = 22/51 (43%)
Frame = +3
Query: 303 LLHXXXXXXXXXSIFLALVMRKRMQKQPESVKKVLRHYADKKSKKPALIAK 455
L H +FL LVM + PE++ +VLRHY + P K
Sbjct: 126 LKHCFFSTTSRDELFLNLVM----EYVPETLYRVLRHYTSSNQRMPIFYVK 172
>At1g06390.1 68414.m00675 shaggy-related protein kinase iota /
ASK-iota (ASK9) (GSK1) identical to shaggy-related
protein kinase iota (ASK-iota) [Arabidopsis thaliana]
SWISS-PROT:Q39012
Length = 407
Score = 28.7 bits (61), Expect = 3.1
Identities = 16/51 (31%), Positives = 22/51 (43%)
Frame = +3
Query: 303 LLHXXXXXXXXXSIFLALVMRKRMQKQPESVKKVLRHYADKKSKKPALIAK 455
L H +FL LVM + PE++ +VLRHY + P K
Sbjct: 126 LKHCFFSTTSRDELFLNLVM----EYVPETLYRVLRHYTSSNQRMPIFYVK 172
>At2g36190.1 68415.m04442 beta-fructosidase, putative /
beta-fructofuranosidase, putative similar to
beta-fructofuranosidase GI:18324 from [Daucus carota]
Length = 591
Score = 28.3 bits (60), Expect = 4.1
Identities = 13/38 (34%), Positives = 22/38 (57%)
Frame = +2
Query: 80 LNELNQYLAERSYVSGYTPSQADIKVFEQVGKVPAASL 193
+N + + +R V G TP+QAD++V VG + A +
Sbjct: 386 MNNHDIKMGQRIEVKGITPAQADVEVTFYVGSLEKAEI 423
>At2g30980.1 68415.m03778 shaggy-related protein kinase delta /
ASK-delta / ASK-dzeta (ASK4) identical to shaggy-related
protein kinase delta (ASK-delta) (ASK- dzeta)
[Arabidopsis thaliana] SWISS-PROT:Q39010
Length = 412
Score = 27.9 bits (59), Expect = 5.5
Identities = 22/84 (26%), Positives = 36/84 (42%), Gaps = 2/84 (2%)
Frame = +3
Query: 210 GTATSHRTPLLNAKPGQRELAH*QLELNPR--PLLHXXXXXXXXXSIFLALVMRKRMQKQ 383
G + + + L + + REL +L +P L H +FL LVM +
Sbjct: 95 GESVAIKKVLQDRRYKNRELQLMRLMDHPNVVSLKHCFFSTTTRDELFLNLVM----EYV 150
Query: 384 PESVKKVLRHYADKKSKKPALIAK 455
PE++ +VL+HY + P K
Sbjct: 151 PETLYRVLKHYTSSNQRMPIFYVK 174
>At2g44460.1 68415.m05528 glycosyl hydrolase family 1 protein
contains Pfam PF00232 : Glycosyl hydrolase family 1
domain; TIGRFAM TIGR01233: 6-phospho-beta-galactosidase;
similar to beta-glucosidase 1 (GI:12043529) [Arabidopsis
thaliana]
Length = 582
Score = 27.5 bits (58), Expect = 7.2
Identities = 16/46 (34%), Positives = 25/46 (54%)
Frame = +3
Query: 372 MQKQPESVKKVLRHYADKKSKKPALIAKSSXILDVXPWDDETDMKE 509
+Q PE ++KVL + DK + P + K + I D +DD T +E
Sbjct: 385 LQSHPEGLRKVLNYIKDKYN-NPIVYIKENGIND---YDDGTKSRE 426
>At2g05170.1 68415.m00544 vacuolar protein sorting 11 family protein
/ VPS11 family protein similar to Vacuolar protein
sorting 11 (hVPS11) (PP3476) (Swiss-Prot:Q9H270) [Homo
sapiens]; similar to Vacuolar biogenesis protein END1
(PEP5 protein) (Vacuolar protein sorting 11)
(Swiss-Prot:P12868) [Saccharomyces cerevisiae]
Length = 932
Score = 27.5 bits (58), Expect = 7.2
Identities = 14/33 (42%), Positives = 18/33 (54%)
Frame = +2
Query: 80 LNELNQYLAERSYVSGYTPSQADIKVFEQVGKV 178
L +L Y YVS PSQA + + EQ GK+
Sbjct: 495 LEDLGNYDEALQYVSSLEPSQAGVTI-EQYGKI 526
>At5g01280.1 68418.m00037 expressed protein
Length = 460
Score = 27.1 bits (57), Expect = 9.6
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +3
Query: 369 RMQKQPESVKKVLRHYADKKSKKPALIAKSSXILDVXPWD 488
R P VLR K KPAL ++S I+ PW+
Sbjct: 201 RRPSTPNGSSTVLRSKPTKPLSKPALSLEASPIVRSRPWE 240
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,234,778
Number of Sequences: 28952
Number of extensions: 187735
Number of successful extensions: 521
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 511
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 520
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1197101088
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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