BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_G03
(595 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC777.15 |||tRNA dihydrouridine synthase Dus4 |Schizosaccharom... 31 0.17
SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomy... 28 0.89
SPAC1687.12c |coq4||ubiquinone biosynthesis protein Coq4|Schizos... 28 1.2
SPCC1620.08 |||succinate-CoA ligase |Schizosaccharomyces pombe|c... 27 1.6
SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces pom... 27 2.1
SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyc... 26 3.6
SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces ... 25 6.3
SPAC1783.07c |pap1|caf3, caf3|transcription factor Caf3|Schizosa... 25 6.3
SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual 25 6.3
SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase E3|Schizosac... 25 6.3
SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces... 25 8.3
SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosacch... 25 8.3
>SPCC777.15 |||tRNA dihydrouridine synthase Dus4
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 326
Score = 30.7 bits (66), Expect = 0.17
Identities = 11/18 (61%), Positives = 13/18 (72%)
Frame = +1
Query: 310 YYLHDYSLNAHYYYHHLT 363
+Y YSLN H +YHHLT
Sbjct: 258 WYSTSYSLNFHLFYHHLT 275
>SPAC19B12.02c |||1,3-beta-glucanosyltransferase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 542
Score = 28.3 bits (60), Expect = 0.89
Identities = 12/28 (42%), Positives = 16/28 (57%)
Frame = -1
Query: 238 ICKWKDSTRSCVIRELQSFGQYAKFHRY 155
+C+ DSTRSCVI + S Y+ Y
Sbjct: 366 VCECMDSTRSCVINDDVSSDDYSDLFSY 393
>SPAC1687.12c |coq4||ubiquinone biosynthesis protein
Coq4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 272
Score = 27.9 bits (59), Expect = 1.2
Identities = 13/34 (38%), Positives = 23/34 (67%)
Frame = +1
Query: 373 WLGGDVVPLLKERRGEWYWFVHKQLVNTLLYGKT 474
W GGD++ +L + G+ + F+H+ L+N +L KT
Sbjct: 74 WRGGDMISVLGDASGQPF-FLHR-LLNKMLVDKT 105
>SPCC1620.08 |||succinate-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 433
Score = 27.5 bits (58), Expect = 1.6
Identities = 16/53 (30%), Positives = 28/53 (52%), Gaps = 4/53 (7%)
Frame = +3
Query: 261 VEYYCLALPLPKRFYVLLLT*LQFKCPLLLSSS----DLQQVARRRCSSSIKR 407
V Y C + K +Y +L + +CP++++S D++ VA S+ IKR
Sbjct: 124 VVYVCERKFIRKEYYFAILMDRENQCPMIVASDQGGVDIETVAAENPSAIIKR 176
>SPAC22H10.03c |kap114||karyopherin Kap14|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 986
Score = 27.1 bits (57), Expect = 2.1
Identities = 9/27 (33%), Positives = 17/27 (62%)
Frame = +1
Query: 109 GYAIPPIYEVLPEYFNNGEILHTAQRI 189
GY +PP+Y++ + + E+L +Q I
Sbjct: 666 GYVLPPVYKITQIHSGDTELLQLSQEI 692
>SPCC1672.10 |mis16||kinetochore protein Mis16 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 430
Score = 26.2 bits (55), Expect = 3.6
Identities = 18/53 (33%), Positives = 26/53 (49%), Gaps = 2/53 (3%)
Frame = -1
Query: 238 ICKWKDSTRSCVIRELQSFGQYAKFHRY*NI--PVKPHILEE*RNLLSQNDEC 86
IC W T+S E + AK+HR+ +I V+ H E L S +D+C
Sbjct: 207 ICLWDVQTQSFTSSETKVISPIAKYHRHTDIVNDVQFHPQHE-ALLASVSDDC 258
>SPCC1494.07 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1502
Score = 25.4 bits (53), Expect = 6.3
Identities = 8/16 (50%), Positives = 12/16 (75%)
Frame = +3
Query: 465 WKDFLMDLVKIGET*W 512
WK+ +M+L+K ET W
Sbjct: 622 WKNIVMELIKASETIW 637
>SPAC1783.07c |pap1|caf3, caf3|transcription factor
Caf3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 552
Score = 25.4 bits (53), Expect = 6.3
Identities = 10/19 (52%), Positives = 11/19 (57%)
Frame = +3
Query: 177 CPKDWSSRITHDRVLSFHL 233
CPK WS I H R SF +
Sbjct: 501 CPKVWSKIINHPRFESFDI 519
>SPAC18B11.05 |gpi18||pig-V|Schizosaccharomyces pombe|chr 1|||Manual
Length = 426
Score = 25.4 bits (53), Expect = 6.3
Identities = 8/22 (36%), Positives = 13/22 (59%)
Frame = +1
Query: 214 EYYPSTYKWDNSVVIRSNTTVW 279
+ YP+ + W + +IRSN W
Sbjct: 205 QQYPAAFLWSLATLIRSNGIFW 226
>SPAC12G12.01c ||SPAC630.02|ubiquitin-protein ligase
E3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 905
Score = 25.4 bits (53), Expect = 6.3
Identities = 30/125 (24%), Positives = 53/125 (42%), Gaps = 7/125 (5%)
Frame = +1
Query: 133 EVLPEYFN--NGEILHTAQRIGVHGSRMIEYYPSTYKWDNSVVIRSNTTVWHYHCQSASM 306
EV+P FN N I +++ S ++D S + N+ C S+ +
Sbjct: 325 EVIPFSFNPYNDLIFSFKEKLYPLNSSPFNTLSDVPQFDVSEFVDENSFDSSSSC-SSKV 383
Query: 307 SYYLHDYSLNAHYYYHH--LTYNKWLGGDVVPLLKERRGEWYWF---VHKQLVNTLLYGK 471
+ S+N+ H L+YN+ LG D+ + +R + Y F + +LV+ L
Sbjct: 384 FLTTRNNSINSEDSAHEVLLSYNRVLGSDIQGTILDRVKKGYQFDSQKNSELVSDLYLKD 443
Query: 472 TF*WI 486
+ WI
Sbjct: 444 LWSWI 448
>SPCC970.08 |||inositol polyphosphate kinase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 967
Score = 25.0 bits (52), Expect = 8.3
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -1
Query: 388 HRLLATCCKSDDDNNSGHLNCNHV 317
H L+ K+DDD ++ HL +H+
Sbjct: 800 HALMRYLGKTDDDEDNSHLLVHHI 823
>SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 474
Score = 25.0 bits (52), Expect = 8.3
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = +1
Query: 292 QSASMSYYLHDYSLNAHYYYHHLTYNKWLG 381
Q+A + Y HD +L H Y H +N W+G
Sbjct: 336 QAAGIFY--HDLALRPHAYQH---FNTWMG 360
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,660,882
Number of Sequences: 5004
Number of extensions: 58157
Number of successful extensions: 144
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 139
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 258201856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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