Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_G01
(520 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g37720.1 68418.m04541 RNA and export factor-binding protein, ... 33 0.087
At5g08580.1 68418.m01021 calcium-binding EF hand family protein ... 33 0.087
At5g13480.1 68418.m01554 WD-40 repeat family protein similar to ... 31 0.47
At5g57660.1 68418.m07205 zinc finger (B-box type) family protein... 30 0.81
At5g24930.1 68418.m02952 zinc finger (B-box type) family protein... 30 1.1
At3g02380.1 68416.m00223 zinc finger protein CONSTANS-LIKE 2 (CO... 30 1.1
At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N) f... 29 2.5
At5g56900.2 68418.m07101 CwfJ-like family protein / zinc finger ... 28 3.3
At5g56900.1 68418.m07100 CwfJ-like family protein / zinc finger ... 28 3.3
At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR... 28 3.3
At4g02430.1 68417.m00329 pre-mRNA splicing factor, putative / SR... 28 3.3
At3g59870.1 68416.m06681 expressed protein hypothetical protein ... 27 5.7
At5g22010.1 68418.m02561 AAA-type ATPase family protein / BRCT d... 27 7.6
At4g29310.1 68417.m04190 expressed protein 27 7.6
>At5g37720.1 68418.m04541 RNA and export factor-binding protein,
putative transcriptional coactivator ALY, Mus musculus,
EMBL:MMU89876
Length = 288
Score = 33.5 bits (73), Expect = 0.087
Identities = 17/47 (36%), Positives = 21/47 (44%)
Frame = -2
Query: 168 SRCTPAFPCKSRSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 28
SR P + R RGG RG GRGRG G + P+ +S
Sbjct: 223 SRRLPIHNQQGGGMRGGRGGFRARGRGNGGRGRGGGRGNGKKPVEKS 269
>At5g08580.1 68418.m01021 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 391
Score = 33.5 bits (73), Expect = 0.087
Identities = 21/53 (39%), Positives = 30/53 (56%), Gaps = 5/53 (9%)
Frame = +3
Query: 336 LDAHDNDRFVSRHELFPIRAPLMSLEHCIAP-----FLDQCDADEDHRITLAE 479
LD +D D ++S EL PI + + EH A + Q D+D+D R+TLAE
Sbjct: 311 LDKND-DGYLSDVELLPIISKIHPTEHYYAKQQADYIISQADSDKDRRLTLAE 362
>At5g13480.1 68418.m01554 WD-40 repeat family protein similar to
WD-repeat protein WDC146 (SP:Q9C0J8|) {Homo sapiens};
contains 3 weak Pfam PF00400: WD domain, G-beta repeats;
Length = 711
Score = 31.1 bits (67), Expect = 0.47
Identities = 13/42 (30%), Positives = 23/42 (54%)
Frame = +2
Query: 71 LPRPLGAVPRGAVPPRADRVLRDLQGNAGVQRERNVRLPAAH 196
LPRP+ P G +PP + + + G+ G+Q N ++ +H
Sbjct: 608 LPRPMQMPPHGHMPPPSMPMSHQMPGSMGMQGGMNPQMSQSH 649
>At5g57660.1 68418.m07205 zinc finger (B-box type) family protein
contains Pfam domain, PF00643: B-box zinc finger
Length = 355
Score = 30.3 bits (65), Expect = 0.81
Identities = 16/50 (32%), Positives = 24/50 (48%)
Frame = +3
Query: 276 EAESNLTRRWTNAAIWKWCDLDAHDNDRFVSRHELFPIRAPLMSLEHCIA 425
+A + +T + AA+ CD D H + SRHE P+ S E +A
Sbjct: 66 QAPAAVTCKADAAALCVSCDADIHSANPLASRHERVPVETFFDSAETAVA 115
>At5g24930.1 68418.m02952 zinc finger (B-box type) family protein
similar to CONSTANS-like protein 1 GI:4091804 from
[Malus x domestica]
Length = 406
Score = 29.9 bits (64), Expect = 1.1
Identities = 19/63 (30%), Positives = 29/63 (46%), Gaps = 7/63 (11%)
Frame = +3
Query: 276 EAESNLTRRWTNAAIWKWCDLDAHDNDRFVSRHELFPIRAPLM-------SLEHCIAPFL 434
+A +++T + AA+ CD D H + RHE P+ P S++H FL
Sbjct: 98 QAPAHVTCKADAAALCVTCDRDIHSANPLARRHERVPV-TPFYDSVSSDGSVKHTAVNFL 156
Query: 435 DQC 443
D C
Sbjct: 157 DDC 159
>At3g02380.1 68416.m00223 zinc finger protein CONSTANS-LIKE 2 (COL2)
identical to putative flowering-time gene CONSTANS
(COL2) GB:AAB67879 GI:1507699 SP:Q96502 (Arabidopsis
thaliana)
Length = 347
Score = 29.9 bits (64), Expect = 1.1
Identities = 21/59 (35%), Positives = 28/59 (47%), Gaps = 2/59 (3%)
Frame = +3
Query: 312 AAIWKWCDLDAHDNDRFVSRHELFPIRAPLMSLEHC--IAPFLDQCDADEDHRITLAEW 482
A++ CD + H + RH+ PI PL S C +AP D DED R +A W
Sbjct: 76 ASLCTACDAEIHSANPLARRHQRVPI-LPL-SANSCSSMAPSETDADNDEDDR-EVASW 131
>At3g13490.1 68416.m01697 tRNA synthetase class II (D, K and N)
family protein similar to SP|Q9RHV9 Lysyl-tRNA
synthetase (EC 6.1.1.6) (Lysine--tRNA ligase) {Bacillus
stearothermophilus}; contains Pfam profile: PF00152 tRNA
synthetases class II (D, K and N)
Length = 602
Score = 28.7 bits (61), Expect = 2.5
Identities = 13/33 (39%), Positives = 18/33 (54%)
Frame = -2
Query: 159 TPAFPCKSRSTRSARGGTAPRGTAPSGRGRGSA 61
+PA C S ++ S+ T + PSGR R SA
Sbjct: 40 SPALRCASAASSSSSSATTAETSKPSGRNRRSA 72
>At5g56900.2 68418.m07101 CwfJ-like family protein / zinc finger
(CCCH-type) family protein contains Pfam domain,
PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and
similar), PF04677: Protein similar to CwfJ C-terminus 1,
PF04676: Protein similar to CwfJ C-terminus 2
Length = 593
Score = 28.3 bits (60), Expect = 3.3
Identities = 30/111 (27%), Positives = 47/111 (42%), Gaps = 2/111 (1%)
Frame = -1
Query: 340 SRSHHFQMAALVQRRVRLDSASRSMRR*WGVSSRRSARSRIMLNSQSRMRRGKSDISFSL 161
S H FQ + +QR+ R ++A+RS + W S S S ++++ SL
Sbjct: 354 SYKHEFQDESSIQRKPRSENANRS-KECWFCLSSPSVESHLIVSVGESFYCALP--KGSL 410
Query: 160 HSGISLQVP*YSICTWWYCAPRHSSEWSRQRQRCR--YTSQSDDQVSLKFV 14
L +P + +P SE SR + R Y SQ +D V + V
Sbjct: 411 VEDHILIIPIEHLPNTLVLSPEVESELSRYQNGLRNCYKSQGNDAVFFELV 461
>At5g56900.1 68418.m07100 CwfJ-like family protein / zinc finger
(CCCH-type) family protein contains Pfam domain,
PF00642: Zinc finger C-x8-C-x5-C-x3-H type (and
similar), PF04677: Protein similar to CwfJ C-terminus 1,
PF04676: Protein similar to CwfJ C-terminus 2
Length = 404
Score = 28.3 bits (60), Expect = 3.3
Identities = 30/111 (27%), Positives = 47/111 (42%), Gaps = 2/111 (1%)
Frame = -1
Query: 340 SRSHHFQMAALVQRRVRLDSASRSMRR*WGVSSRRSARSRIMLNSQSRMRRGKSDISFSL 161
S H FQ + +QR+ R ++A+RS + W S S S ++++ SL
Sbjct: 165 SYKHEFQDESSIQRKPRSENANRS-KECWFCLSSPSVESHLIVSVGESFYCALP--KGSL 221
Query: 160 HSGISLQVP*YSICTWWYCAPRHSSEWSRQRQRCR--YTSQSDDQVSLKFV 14
L +P + +P SE SR + R Y SQ +D V + V
Sbjct: 222 VEDHILIIPIEHLPNTLVLSPEVESELSRYQNGLRNCYKSQGNDAVFFELV 272
>At4g02430.2 68417.m00330 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 278
Score = 28.3 bits (60), Expect = 3.3
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = -2
Query: 135 RSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 28
RS+ ARG + RG GRG G G R P RS
Sbjct: 85 RSSHDARGSYSGRGRG--GRGGGDGGGRERGPSRRS 118
Score = 27.9 bits (59), Expect = 4.3
Identities = 24/61 (39%), Positives = 32/61 (52%), Gaps = 1/61 (1%)
Frame = -2
Query: 204 SRACAAGSLTFRSRCTPAFPCKSRS-TRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 28
SR+ + G +SR P +SRS +RS +P+ A S R R A T RSP +RS
Sbjct: 199 SRSPSRGRSYSKSRSRGRSPSRSRSRSRSRSKSRSPK--AKSLR-RSPAKSTSRSPRSRS 255
Query: 27 R 25
R
Sbjct: 256 R 256
>At4g02430.1 68417.m00329 pre-mRNA splicing factor, putative / SR1
protein, putative strong similarity to SP|O22315
Pre-mRNA splicing factor SF2 (SR1 protein) {Arabidopsis
thaliana}; cDNA NCBI_gi:15810292 supports a truncated
version while protein evidence supports a longer model.
Length = 178
Score = 28.3 bits (60), Expect = 3.3
Identities = 16/36 (44%), Positives = 18/36 (50%)
Frame = -2
Query: 135 RSTRSARGGTAPRGTAPSGRGRGSAVGTPRSPMTRS 28
RS+ ARG + RG GRG G G R P RS
Sbjct: 85 RSSHDARGSYSGRGRG--GRGGGDGGGRERGPSRRS 118
>At3g59870.1 68416.m06681 expressed protein hypothetical protein
F6E13.7 - Arabidopsis thaliana, PIR:T00674
Length = 288
Score = 27.5 bits (58), Expect = 5.7
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = -1
Query: 370 RDTNLSLSCASRSHHFQMAALVQRRVRLDSASRSM 266
R ++ S SC+S+S FQ+ ++ +RR R S S M
Sbjct: 33 RRSSSSTSCSSQSQSFQLFSVRRRRSRSSSPSIPM 67
>At5g22010.1 68418.m02561 AAA-type ATPase family protein / BRCT
domain-containing protein contains Pfam profiles:
PF00533 BRCA1 C Terminus (BRCT) domain, PF00004 ATPase
family associated with various cellular activities (AAA)
Length = 956
Score = 27.1 bits (57), Expect = 7.6
Identities = 12/27 (44%), Positives = 14/27 (51%)
Frame = -2
Query: 135 RSTRSARGGTAPRGTAPSGRGRGSAVG 55
+S RGG A G + GRGRG G
Sbjct: 154 KSAGRGRGGRAAPGASTGGRGRGGGRG 180
>At4g29310.1 68417.m04190 expressed protein
Length = 424
Score = 27.1 bits (57), Expect = 7.6
Identities = 12/28 (42%), Positives = 14/28 (50%)
Frame = -2
Query: 156 PAFPCKSRSTRSARGGTAPRGTAPSGRG 73
P F CK S R+ R + P G S RG
Sbjct: 186 PVFSCKFSSDRNGRSRSLPSGFTYSSRG 213
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.315 0.123 0.449
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,819,802
Number of Sequences: 28952
Number of extensions: 191149
Number of successful extensions: 733
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 688
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 733
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 947539968
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
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