Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_F24
(437 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g29630.2 68415.m03600 thiamine biosynthesis family protein / ... 28 3.2
At2g29630.1 68415.m03599 thiamine biosynthesis family protein / ... 28 3.2
At5g13130.1 68418.m01504 hypothetical protein low similarity to ... 27 4.2
At5g07760.1 68418.m00888 formin homology 2 domain-containing pro... 27 4.2
At1g27850.1 68414.m03413 expressed protein similar to En/Spm-lik... 27 4.2
At2g35630.1 68415.m04369 microtubule organization 1 protein (MOR... 27 7.3
At1g13110.1 68414.m01520 cytochrome P450 71B7 (CYP71B7) identica... 27 7.3
At4g22190.1 68417.m03208 expressed protein 26 9.7
>At2g29630.2 68415.m03600 thiamine biosynthesis family protein /
thiC family protein contains Pfam profile: PF01964 ThiC
family
Length = 644
Score = 27.9 bits (59), Expect = 3.2
Identities = 26/84 (30%), Positives = 40/84 (47%), Gaps = 5/84 (5%)
Frame = -1
Query: 395 PTHVPLHKLPGATGIQLLTAG-IVYLTLGPIVGWIK---DNASTAVTLHCLNIFTWMTAV 228
P HVP+HK+P QL + TLGP+ I D+ ++A+ NI A+
Sbjct: 424 PGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAI--GAANI----GAL 477
Query: 227 SWGLQKYITTRRQ-NISNKDTIKA 159
L Y+T + + N+D +KA
Sbjct: 478 GTALLCYVTPKEHLGLPNRDDVKA 501
>At2g29630.1 68415.m03599 thiamine biosynthesis family protein /
thiC family protein contains Pfam profile: PF01964 ThiC
family
Length = 644
Score = 27.9 bits (59), Expect = 3.2
Identities = 26/84 (30%), Positives = 40/84 (47%), Gaps = 5/84 (5%)
Frame = -1
Query: 395 PTHVPLHKLPGATGIQLLTAG-IVYLTLGPIVGWIK---DNASTAVTLHCLNIFTWMTAV 228
P HVP+HK+P QL + TLGP+ I D+ ++A+ NI A+
Sbjct: 424 PGHVPMHKIPENMQKQLEWCNEAPFYTLGPLTTDIAPGYDHITSAI--GAANI----GAL 477
Query: 227 SWGLQKYITTRRQ-NISNKDTIKA 159
L Y+T + + N+D +KA
Sbjct: 478 GTALLCYVTPKEHLGLPNRDDVKA 501
>At5g13130.1 68418.m01504 hypothetical protein low similarity to
microrchidia [Mus musculus] GI:5410255
Length = 706
Score = 27.5 bits (58), Expect = 4.2
Identities = 14/56 (25%), Positives = 23/56 (41%)
Frame = -3
Query: 405 FSYSNARTFT*IAWSNWHTIANSWNSISHIRTNSWLDKRQCFHSGNIALSKHIYLD 238
F Y + + ++ + N W I + TN WLD + + LS+ LD
Sbjct: 230 FLYETRKCEAIVPTVDYELVDNKWKEIVYNSTNEWLDNLETILRWSPYLSQQDLLD 285
>At5g07760.1 68418.m00888 formin homology 2 domain-containing
protein / FH2 domain-containing protein contains formin
homology 2 domain, Pfam:PF02181
Length = 853
Score = 27.5 bits (58), Expect = 4.2
Identities = 14/50 (28%), Positives = 28/50 (56%), Gaps = 2/50 (4%)
Frame = -3
Query: 354 HTIANSWNSISHIRTNSWLDKRQCFHSGNIALSKHIYLDD--CSILGFTE 211
H + NSW + +IR ++ +D+R+ F++ + + L D ++LG E
Sbjct: 506 HVVHNSWLNNLNIRASTLIDQRRAFNTMIMLQKVEMPLPDMMAAVLGMDE 555
>At1g27850.1 68414.m03413 expressed protein similar to En/Spm-like
transposon protein GB:AAB95292 GI:2088658 from
[Arabidopsis thaliana]
Length = 1148
Score = 27.5 bits (58), Expect = 4.2
Identities = 14/43 (32%), Positives = 25/43 (58%)
Frame = -3
Query: 417 SFHGFSYSNARTFT*IAWSNWHTIANSWNSISHIRTNSWLDKR 289
S+ GFSYS R+ + + + + ++SW+ S IR S + +R
Sbjct: 726 SYEGFSYSRDRSISLRSSTETASASSSWDYGSSIRKGSHIRQR 768
>At2g35630.1 68415.m04369 microtubule organization 1 protein (MOR1)
identical to microtubule organization 1 protein
GI:14317953 from [Arabidopsis thaliana]
Length = 1978
Score = 26.6 bits (56), Expect = 7.3
Identities = 11/30 (36%), Positives = 18/30 (60%)
Frame = +1
Query: 166 IVSLLDIF*RLVVMYFCKPQDTAVIQVNMF 255
I+ +LD+ R V+ FCK T +++V F
Sbjct: 1206 IIEVLDVLLRWFVLQFCKSNTTCLLKVLEF 1235
>At1g13110.1 68414.m01520 cytochrome P450 71B7 (CYP71B7) identical
to (SP:Q96514) cytochrome P450 71B7 [Arabidopsis
thaliana]; PF|00067 Cytochrome P450 family. ESTs
gb|T44875, gb|T04814, gb|R65111, gb|T44310 and gb|T04541
come from this gene; identical to cDNA cytochrome P450
GI:1523795, ATCYP71B7
Length = 504
Score = 26.6 bits (56), Expect = 7.3
Identities = 20/64 (31%), Positives = 31/64 (48%), Gaps = 2/64 (3%)
Frame = -1
Query: 350 QLLTAGIVYLTLGPIVGWIKDNAS-TAVTLHCLNIFTWM-TAVSWGLQKYITTRRQNISN 177
++L AG+ + P VGW+ D S TL+ N+F+ + T L +I RQ N
Sbjct: 211 EMLVAGVAFTDFFPGVGWLVDRISGQNKTLN--NVFSELDTFFQNVLDDHIKPGRQVSEN 268
Query: 176 KDTI 165
D +
Sbjct: 269 PDVV 272
>At4g22190.1 68417.m03208 expressed protein
Length = 387
Score = 26.2 bits (55), Expect = 9.7
Identities = 10/35 (28%), Positives = 18/35 (51%)
Frame = +2
Query: 248 ICLDSAMLPLWKHCLLSNQLLVLM*DILFQLLAIV 352
+C L +W HC S+ L L +++ LL ++
Sbjct: 1 MCYVPQTLHIWSHCTCSSSFLSLTNSLIYYLLLLL 35
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,583,054
Number of Sequences: 28952
Number of extensions: 142429
Number of successful extensions: 345
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 342
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 345
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 692941200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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