BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_E22
(358 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g17270.1 68414.m02103 expressed protein 27 2.8
At1g59670.1 68414.m06711 glutathione S-transferase, putative sim... 27 4.9
At4g19720.1 68417.m02896 glycosyl hydrolase family 18 protein si... 26 6.4
At1g68110.1 68414.m07780 epsin N-terminal homology (ENTH) domain... 26 6.4
At1g48390.1 68414.m05405 syntaxin-related family protein contain... 26 6.4
At1g06720.1 68414.m00714 expressed protein contains Pfam domain,... 26 6.4
At5g40530.2 68418.m04918 expressed protein contains Pfam profile... 26 8.5
At5g40530.1 68418.m04919 expressed protein contains Pfam profile... 26 8.5
>At1g17270.1 68414.m02103 expressed protein
Length = 564
Score = 27.5 bits (58), Expect = 2.8
Identities = 16/60 (26%), Positives = 30/60 (50%)
Frame = +2
Query: 50 LSALVTQASLTPLSNLTVAFINYEELSPLNNINSYEQLYDSVVVGDYKAAVMKTLRLEND 229
+S+ V ++ L L+ L + L N NS + SVV+ + KAA++K + + +
Sbjct: 102 MSSRVKESELQALNLLRQQQLALVSLLNRTNFNSSNAISSSVVIDNVKAALLKQISVNKE 161
>At1g59670.1 68414.m06711 glutathione S-transferase, putative
similar to glutathione S-transferase GB:AAF29773
GI:6856103 from [Gossypium hirsutum]
Length = 233
Score = 26.6 bits (56), Expect = 4.9
Identities = 10/21 (47%), Positives = 14/21 (66%)
Frame = +2
Query: 287 NIVEYAYKLWNMFGTNIVKDH 349
NIVEY + WN G++I+ H
Sbjct: 72 NIVEYIDETWNSSGSSILPSH 92
>At4g19720.1 68417.m02896 glycosyl hydrolase family 18 protein
similar to chitinase, class V GI:899342 from [Nicotiana
tabacum]
Length = 363
Score = 26.2 bits (55), Expect = 6.4
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = +2
Query: 110 INYEELSPLNNINSYEQLYDSVVVGDY 190
INY+++ L + ++DS VVGDY
Sbjct: 272 INYDQIKRLIVDHKARPVFDSTVVGDY 298
>At1g68110.1 68414.m07780 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related similar to clathrin assembly protein
AP180 (GI:6492344) [Xenopus laevis]; contains Pfam
profile: PF01417 ENTH domain, suggesting involvement in
endocytosis or cytoskeletal machinery
Length = 379
Score = 26.2 bits (55), Expect = 6.4
Identities = 13/44 (29%), Positives = 22/44 (50%)
Frame = +2
Query: 176 VVGDYKAAVMKTLRLENDGQGQVINLVVNRLLSEGKRNIVEYAY 307
+ G AVMK L L + + +VN+ S+G+ IV + +
Sbjct: 219 IYGRICGAVMKVLPLAGKSEAATVLKIVNKTTSQGEDLIVYFEF 262
>At1g48390.1 68414.m05405 syntaxin-related family protein contains a
novel domain similar to F-box that is shared among other
proteins in Arabidopsis; similar to proteins At3g54160,
At1g47920 (syntaxin SYP81), At5g41830, At3g44180,
At3g58890, At1g56610, At1g48390, At3g59270 [Arabidopsis
thaliana]
Length = 413
Score = 26.2 bits (55), Expect = 6.4
Identities = 11/33 (33%), Positives = 17/33 (51%)
Frame = +3
Query: 75 PLHL*ATSRLHLLTTKSFRHSITSTLTNSCTTV 173
PL + + LH L K F H +TS ++C +
Sbjct: 307 PLLIRSCPNLHTLVIKGFVHRVTSKCGDACACI 339
>At1g06720.1 68414.m00714 expressed protein contains Pfam domain,
PF04950: Protein of unknown function (DUF663)
Length = 1147
Score = 26.2 bits (55), Expect = 6.4
Identities = 12/45 (26%), Positives = 19/45 (42%)
Frame = +2
Query: 107 FINYEELSPLNNINSYEQLYDSVVVGDYKAAVMKTLRLENDGQGQ 241
F+NY L E + D GD+ A ++ L G+G+
Sbjct: 619 FVNYGYLKNWKEKEVCESIRDRFTTGDWSKAALRDKNLGTGGEGE 663
>At5g40530.2 68418.m04918 expressed protein contains Pfam profile
PF05148: Protein of unknown function (DUF691)
Length = 301
Score = 25.8 bits (54), Expect = 8.5
Identities = 16/72 (22%), Positives = 31/72 (43%)
Frame = +1
Query: 4 TTANRDQRNDFNDNGAECTGDASVPYTSKQPHGCIY*LRRAFATQ*HQLLRTVVRQCSCR 183
T N++++ + D + G +S P +P + LR + ++L + CS +
Sbjct: 31 TPKNQNEKKNQRDTKNQQHGGSSAPSKRPKPSNFLDALRERLSGGQFRMLNEKLYTCSGK 90
Query: 184 RLQGCSNEDPQI 219
EDPQ+
Sbjct: 91 EALDYFKEDPQM 102
>At5g40530.1 68418.m04919 expressed protein contains Pfam profile
PF05148: Protein of unknown function (DUF691)
Length = 287
Score = 25.8 bits (54), Expect = 8.5
Identities = 16/72 (22%), Positives = 31/72 (43%)
Frame = +1
Query: 4 TTANRDQRNDFNDNGAECTGDASVPYTSKQPHGCIY*LRRAFATQ*HQLLRTVVRQCSCR 183
T N++++ + D + G +S P +P + LR + ++L + CS +
Sbjct: 31 TPKNQNEKKNQRDTKNQQHGGSSAPSKRPKPSNFLDALRERLSGGQFRMLNEKLYTCSGK 90
Query: 184 RLQGCSNEDPQI 219
EDPQ+
Sbjct: 91 EALDYFKEDPQM 102
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,673,687
Number of Sequences: 28952
Number of extensions: 105521
Number of successful extensions: 327
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 326
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 327
length of database: 12,070,560
effective HSP length: 72
effective length of database: 9,986,016
effective search space used: 459356736
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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