Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_E21
(617 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g75270.1 68414.m08744 dehydroascorbate reductase, putative si... 33 0.15
At2g30870.1 68415.m03762 glutathione S-transferase, putative sup... 33 0.20
At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative / ... 32 0.26
At4g28910.2 68417.m04132 expressed protein 30 1.1
At4g28910.1 68417.m04131 expressed protein 30 1.1
At2g42880.1 68415.m05309 mitogen-activated protein kinase, putat... 30 1.4
At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative / ... 29 2.5
At1g34460.1 68414.m04281 cyclin, putative strong similarity to c... 29 3.3
At1g02940.1 68414.m00261 glutathione S-transferase, putative sim... 28 4.3
At4g10180.1 68417.m01668 light-mediated development protein 1 / ... 28 5.7
At2g20860.1 68415.m02458 lipoic acid synthase (LIP1) identical t... 28 5.7
At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1... 27 7.5
At1g49860.1 68414.m05590 glutathione S-transferase, putative sim... 27 7.5
At5g17390.1 68418.m02040 universal stress protein (USP) family p... 27 10.0
At3g62760.1 68416.m07050 glutathione S-transferase, putative Glu... 27 10.0
At3g50380.1 68416.m05511 expressed protein 27 10.0
At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1... 27 10.0
At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1... 27 10.0
At1g75110.1 68414.m08723 expressed protein 27 10.0
>At1g75270.1 68414.m08744 dehydroascorbate reductase, putative
similar to GI:6939839 from [Oryza sativa]
Length = 213
Score = 33.1 bits (72), Expect = 0.15
Identities = 35/141 (24%), Positives = 60/141 (42%), Gaps = 2/141 (1%)
Frame = +3
Query: 132 FEDHRVLSADWPD-FKPSTPFAQMPVLVIDGKQYAQSTAICRYLGRKYGIAGANEEESFE 308
++ H + +D P F +P ++PV+ +DGK A S I L KY E S +
Sbjct: 37 YKTHLINVSDKPQWFLDISPEGKVPVVKLDGKWVADSDVIVGLLEEKY------PEPSLK 90
Query: 309 IDQNVEFLHDIRAKSASVFYEADEELKAKKHEDFAKNVYPDMLEKIHAIIKKNNGHIAAG 488
+ + + +F LK+K D ++ D LE + +K ++G AG
Sbjct: 91 TPP------EFASVGSKIFGAFVTFLKSKDANDGSEKALVDELEALENHLKTHSGPFVAG 144
Query: 489 -KLTWGDFVFTGMFEYLKMML 548
K+T D +L++ L
Sbjct: 145 EKITAVDLSLAPKLYHLEVAL 165
>At2g30870.1 68415.m03762 glutathione S-transferase, putative
supported by cDNA GI:443698 GB:D17673
Length = 215
Score = 32.7 bits (71), Expect = 0.20
Identities = 21/64 (32%), Positives = 32/64 (50%), Gaps = 1/64 (1%)
Frame = +3
Query: 165 PDFKPSTPFAQMPVLVIDGKQYAQSTAICRYLGRKYGIAGAN-EEESFEIDQNVEFLHDI 341
P++ PF ++PVLV + +S AI RY+ KY G + ++ E VE D+
Sbjct: 42 PEYLAIQPFGKIPVLVDGDYKIFESRAIMRYIAEKYRSQGPDLLGKTIEERGQVEQWLDV 101
Query: 342 RAKS 353
A S
Sbjct: 102 EATS 105
>At1g57720.1 68414.m06549 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative similar to elongation factor 1B
gamma GI:3868758 from [Oryza sativa]
Length = 413
Score = 32.3 bits (70), Expect = 0.26
Identities = 19/58 (32%), Positives = 30/58 (51%), Gaps = 3/58 (5%)
Frame = +3
Query: 165 PDFKPSTPFAQMPVLVIDGKQYAQSTAICRYLGRKYG---IAGANEEESFEIDQNVEF 329
P+F P ++PVL +S AI RY+ RK G + G++ E I+Q ++F
Sbjct: 43 PEFLKMNPIGKVPVLETPEGPIFESNAIARYVSRKNGDNSLNGSSLIEYAHIEQWIDF 100
>At4g28910.2 68417.m04132 expressed protein
Length = 425
Score = 30.3 bits (65), Expect = 1.1
Identities = 16/52 (30%), Positives = 30/52 (57%)
Frame = +3
Query: 264 RKYGIAGANEEESFEIDQNVEFLHDIRAKSASVFYEADEELKAKKHEDFAKN 419
RK+G G N+++ E D + +H+ + K++ V DE A ++ED A++
Sbjct: 116 RKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTATDEGSTA-ENEDVAES 166
>At4g28910.1 68417.m04131 expressed protein
Length = 425
Score = 30.3 bits (65), Expect = 1.1
Identities = 16/52 (30%), Positives = 30/52 (57%)
Frame = +3
Query: 264 RKYGIAGANEEESFEIDQNVEFLHDIRAKSASVFYEADEELKAKKHEDFAKN 419
RK+G G N+++ E D + +H+ + K++ V DE A ++ED A++
Sbjct: 116 RKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTATDEGSTA-ENEDVAES 166
>At2g42880.1 68415.m05309 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK20) mitogen-activated protein
kinase (MAPK)(AtMPK20), PMID:12119167
Length = 606
Score = 29.9 bits (64), Expect = 1.4
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +1
Query: 226 NMPRAQQSAGT*AVNTVSPEQTKRNPSKSIRTLNSCMTFAP 348
N+P +QQSA T A+ +T P K R ++ +AP
Sbjct: 495 NLPFSQQSAATVAMGKQQERRTTMEPEKQARQISQYNRYAP 535
>At1g09640.1 68414.m01081 elongation factor 1B-gamma, putative /
eEF-1B gamma, putative Similar to elongation factor
1-gamma (gb|EF1G_XENLA). ESTs
gb|T20564,gb|T45940,gb|T04527 come from this gene
Length = 414
Score = 29.1 bits (62), Expect = 2.5
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 3/58 (5%)
Frame = +3
Query: 165 PDFKPSTPFAQMPVLVIDGKQYAQSTAICRYLGRKYG---IAGANEEESFEIDQNVEF 329
P F P ++PVL +S AI RY+ R G + G++ E +I+Q ++F
Sbjct: 43 PAFLKMNPIGKVPVLETPEGSVFESNAIARYVSRLNGDNSLNGSSLIEYAQIEQWIDF 100
>At1g34460.1 68414.m04281 cyclin, putative strong similarity to
cyclin [Arabidopsis thaliana] GI:1360646
Length = 504
Score = 28.7 bits (61), Expect = 3.3
Identities = 23/86 (26%), Positives = 41/86 (47%)
Frame = +3
Query: 201 PVLVIDGKQYAQSTAICRYLGRKYGIAGANEEESFEIDQNVEFLHDIRAKSASVFYEADE 380
PV VI+ K+ AQS A C GI N+ + +ID++ + H + Y +
Sbjct: 231 PVEVIETKRNAQSKAAC-------GI--VNKPKILDIDESDKDNHVAAVEYVDDMYSFYK 281
Query: 381 ELKAKKHEDFAKNVYPDMLEKIHAII 458
E++ + ++ +M EK+ AI+
Sbjct: 282 EVEKESQPKMYMHIQTEMNEKMRAIL 307
>At1g02940.1 68414.m00261 glutathione S-transferase, putative
similar to glutathione S-transferase GI:860955 from
[Hyoscyamus muticus]
Length = 256
Score = 28.3 bits (60), Expect = 4.3
Identities = 12/32 (37%), Positives = 17/32 (53%)
Frame = +3
Query: 165 PDFKPSTPFAQMPVLVIDGKQYAQSTAICRYL 260
P F PF Q+PV + G + +S AI Y+
Sbjct: 79 PSFLAINPFGQVPVFLDGGLKLTESRAISEYI 110
>At4g10180.1 68417.m01668 light-mediated development protein 1 /
deetiolated1 (DET1) identical to Light-mediated
development protein DET1 (Deetiolated1)
(Swiss-Prot:P48732) [Arabidopsis thaliana]
Length = 543
Score = 27.9 bits (59), Expect = 5.7
Identities = 10/29 (34%), Positives = 21/29 (72%)
Frame = -1
Query: 467 ILLYDGVNLLQHIGVYVFREVLMFLRLQF 381
+ L+D VNL ++GV+++ ++L L L++
Sbjct: 180 VFLHDFVNLAHNMGVFLYDDLLAILSLRY 208
>At2g20860.1 68415.m02458 lipoic acid synthase (LIP1) identical to
gi:3928758 contains Pfam profile PF04055: radical SAM
domain protein
Length = 374
Score = 27.9 bits (59), Expect = 5.7
Identities = 26/115 (22%), Positives = 52/115 (45%), Gaps = 7/115 (6%)
Frame = +3
Query: 228 YAQSTAICRYLGRKYGIAGANEEESFEI------DQNVEFLHDIRAKSASVFYEADEELK 389
+ QS + R + ++Y AG + S + DQ V+ + +RA V
Sbjct: 252 FKQSLDVLR-MAKEYAPAGTLTKTSVMLGCGETPDQVVKTMEKVRAAGVDVMTFGQYMRP 310
Query: 390 AKKHEDFAKNVYPDMLEKIHAI-IKKNNGHIAAGKLTWGDFVFTGMFEYLKMMLQ 551
+K+H A+ V PD E+ + ++ ++A+G + + G + Y+K M++
Sbjct: 311 SKRHMPVAEYVTPDAFERYRLLGMEMGFRYVASGPMVRSSYK-AGEY-YIKSMIE 363
>At2g02390.3 68415.m00179 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 228
Score = 27.5 bits (58), Expect = 7.5
Identities = 18/50 (36%), Positives = 20/50 (40%)
Frame = +3
Query: 123 GQDFEDHRVLSADWPDFKPSTPFAQMPVLVIDGKQYAQSTAICRYLGRKY 272
G F+ V D DFK P +P LV S AI YL KY
Sbjct: 44 GDQFDS--VYRFDLQDFKKINPMGTVPALVDGDVVINDSFAIIMYLDEKY 91
>At1g49860.1 68414.m05590 glutathione S-transferase, putative
similar to GI:860955 from [Hyoscyamus muticus] (Plant
Physiol. 109 (1), 253-260 (1995))
Length = 254
Score = 27.5 bits (58), Expect = 7.5
Identities = 13/35 (37%), Positives = 18/35 (51%)
Frame = +3
Query: 186 PFAQMPVLVIDGKQYAQSTAICRYLGRKYGIAGAN 290
PF ++PVL + + AI RYL +Y G N
Sbjct: 53 PFGEVPVLEDGDLKLFEPKAITRYLAEQYKDVGTN 87
>At5g17390.1 68418.m02040 universal stress protein (USP) family
protein contains Pfam profile: PF00582 universal stress
protein family
Length = 285
Score = 27.1 bits (57), Expect = 10.0
Identities = 17/55 (30%), Positives = 27/55 (49%)
Frame = +3
Query: 255 YLGRKYGIAGANEEESFEIDQNVEFLHDIRAKSASVFYEADEELKAKKHEDFAKN 419
+LG AG+ EE++ E + E + D + V E +EE K +D AK+
Sbjct: 40 FLGENGDDAGSGEEDTKEANNESEEVEDKEEERVKVIVEEEEE----KEQDDAKS 90
>At3g62760.1 68416.m07050 glutathione S-transferase, putative
Glutathione transferase III(b) - Zea mays, EMBL:AJ010296
Length = 219
Score = 27.1 bits (57), Expect = 10.0
Identities = 13/42 (30%), Positives = 19/42 (45%)
Frame = +3
Query: 165 PDFKPSTPFAQMPVLVIDGKQYAQSTAICRYLGRKYGIAGAN 290
P F PF ++P L D +S AI Y+ K+ G +
Sbjct: 43 PSFLSMNPFGKVPALQDDDLTLFESRAITAYIAEKHRDKGTD 84
>At3g50380.1 68416.m05511 expressed protein
Length = 3071
Score = 27.1 bits (57), Expect = 10.0
Identities = 11/36 (30%), Positives = 23/36 (63%)
Frame = -1
Query: 377 ISLVEHRCGLGANVMQEFNVLIDFEGFLFVCSGDTV 270
+S+ EH+C + ++ + +VL FEG ++ SG+ +
Sbjct: 990 LSVEEHKCLINVDLCKLQSVLFKFEGNIWKSSGNFI 1025
>At2g02390.2 68415.m00177 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 191
Score = 27.1 bits (57), Expect = 10.0
Identities = 21/61 (34%), Positives = 26/61 (42%), Gaps = 4/61 (6%)
Frame = +3
Query: 102 RMLLSYGGQDFE--DHRVLSADW--PDFKPSTPFAQMPVLVIDGKQYAQSTAICRYLGRK 269
R+ L+ G D+E +L D DFK P +P LV S AI YL K
Sbjct: 24 RIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPALVDGDVVINDSFAIIMYLDEK 83
Query: 270 Y 272
Y
Sbjct: 84 Y 84
>At2g02390.1 68415.m00178 glutathione S-transferase zeta 1 (GSTZ1)
(GST18) identical to SP|Q9ZVQ3|GTZ1_ARATH Glutathione
S-transferase zeta-class 1 (EC 2.5.1.18) (AtGSTZ1)
(Maleylacetone isomerase) (EC 5.2.1.-) (MAI)
{Arabidopsis thaliana}; contains Pfam profiles PF02798:
Glutathione S-transferase, N-terminal domain and
PF00043:Glutathione S-transferase, C-terminal domain
Length = 221
Score = 27.1 bits (57), Expect = 10.0
Identities = 21/61 (34%), Positives = 26/61 (42%), Gaps = 4/61 (6%)
Frame = +3
Query: 102 RMLLSYGGQDFE--DHRVLSADW--PDFKPSTPFAQMPVLVIDGKQYAQSTAICRYLGRK 269
R+ L+ G D+E +L D DFK P +P LV S AI YL K
Sbjct: 24 RIALALKGLDYEYIPVNLLKGDQFDSDFKKINPMGTVPALVDGDVVINDSFAIIMYLDEK 83
Query: 270 Y 272
Y
Sbjct: 84 Y 84
>At1g75110.1 68414.m08723 expressed protein
Length = 428
Score = 27.1 bits (57), Expect = 10.0
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = +3
Query: 375 DEELKAKKHEDFAKNVYPDMLEKIHAIIK 461
++ELK K N +PD LE++HA+++
Sbjct: 382 NQELKKLKPVIVHLNYHPDKLERMHAVVE 410
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,326,264
Number of Sequences: 28952
Number of extensions: 279192
Number of successful extensions: 810
Number of sequences better than 10.0: 19
Number of HSP's better than 10.0 without gapping: 789
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 810
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1246162608
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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