Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_E07
(596 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g45620.1 68415.m05672 nucleotidyltransferase family protein l... 31 0.77
At1g60160.1 68414.m06777 potassium transporter family protein si... 29 1.8
At3g02125.1 68416.m00179 hypothetical protein 29 2.4
At4g39400.1 68417.m05577 brassinosteroid insensitive 1 (BRI1) id... 28 5.4
At3g54240.1 68416.m05995 hydrolase, alpha/beta fold family prote... 28 5.4
At3g44340.1 68416.m04764 sec23/sec24 transport family protein co... 28 5.4
At2g26340.1 68415.m03160 expressed protein 28 5.4
At4g32640.1 68417.m04646 sec23/sec24 transport protein-related 27 7.2
At3g07430.1 68416.m00886 YGGT family protein contains Pfam profi... 27 7.2
At2g05990.2 68415.m00652 enoyl-[acyl-carrier protein] reductase ... 27 7.2
At2g05990.1 68415.m00651 enoyl-[acyl-carrier protein] reductase ... 27 7.2
At1g54000.1 68414.m06152 myrosinase-associated protein, putative... 27 7.2
At5g15740.1 68418.m01841 expressed protein contains Pfam PF03138... 27 9.5
At4g29380.1 68417.m04197 protein kinase family protein / WD-40 r... 27 9.5
At3g48110.2 68416.m05246 aminoacyl-t-RNA synthetase, putative si... 27 9.5
At3g48110.1 68416.m05245 aminoacyl-t-RNA synthetase, putative si... 27 9.5
At3g44330.1 68416.m04763 expressed protein 27 9.5
>At2g45620.1 68415.m05672 nucleotidyltransferase family protein low
similarity to SP|O13833| Caffeine-induced death protein
1 {Schizosaccharomyces pombe}; contains Pfam profiles
PF03828: PAP/25A associated domain, PF01909:
Nucleotidyltransferase domain
Length = 764
Score = 30.7 bits (66), Expect = 0.77
Identities = 32/96 (33%), Positives = 49/96 (51%), Gaps = 9/96 (9%)
Frame = +2
Query: 137 LKEGQEMFLGADTLLA---PAALLGIKSSIGTSFNLFPKLARDILDAIENNDIAKARALQ 307
L++ +++ + L+A P A L + S SF FPK D+ AIE +DI K+ L
Sbjct: 452 LEKQRQLMAHLENLVAKEWPHAKLYLYGSCANSFG-FPKSDIDVCLAIEGDDINKSEMLL 510
Query: 308 EKLSLAIEAHTAEG------AWVPIMKAGMEIATGI 397
KL+ +E+ + A VPI+K M+ TGI
Sbjct: 511 -KLAEILESDNLQNVQALTRARVPIVKL-MDPVTGI 544
>At1g60160.1 68414.m06777 potassium transporter family protein
similar to potassium transporter HAK2p [Mesembryanthemum
crystallinum] gi|14091471|gb|AAK53759; KUP/HAK/KT
Transporter family member, PMID:11500563; contains Pfam
profile PF02705: K+ potassium transporter
Length = 827
Score = 29.5 bits (63), Expect = 1.8
Identities = 13/41 (31%), Positives = 21/41 (51%)
Frame = -3
Query: 396 IPVAISIPAFIIGTHAPSAVCASMAKLSFSCKALALAMSLF 274
+P ++ P F+I T A +M +FSC A+A+ F
Sbjct: 399 VPKSLFWPVFVIATLAAMIASQAMISATFSCVKQAMALGCF 439
>At3g02125.1 68416.m00179 hypothetical protein
Length = 355
Score = 29.1 bits (62), Expect = 2.4
Identities = 26/80 (32%), Positives = 35/80 (43%)
Frame = -3
Query: 357 THAPSAVCASMAKLSFSCKALALAMSLFSIASSISRASLGNRLNEVPILDFMPRSAAGAN 178
T PS+V +S S +LA SLFSI S RL E+P + N
Sbjct: 92 TRIPSSVFSSKPGNSTEW-SLASNESLFSIHDGNFSISTALRLAEIPRFEETVHVITEIN 150
Query: 177 SVSAPRNISWPSLRERKTWA 118
SV P + P+ E++T A
Sbjct: 151 SVPLPPPVKKPNEYEKETIA 170
>At4g39400.1 68417.m05577 brassinosteroid insensitive 1 (BRI1)
identical to GI:2392895
Length = 1196
Score = 27.9 bits (59), Expect = 5.4
Identities = 25/112 (22%), Positives = 46/112 (41%)
Frame = -3
Query: 507 LSYSFYLVNPKLLNFVWIRCFASADIGLCGSDGGPTLIPVAISIPAFIIGTHAPSAVCAS 328
L+ F V+ LL+ + ++ + GS G + S+ + + ++ S +
Sbjct: 84 LNVGFSAVSSSLLSLTGLESLFLSNSHINGSVSG---FKCSASLTSLDLSRNSLSGPVTT 140
Query: 327 MAKLSFSCKALALAMSLFSIASSISRASLGNRLNEVPILDFMPRSAAGANSV 172
+ L SC L + + S G +LN + +LD S +GAN V
Sbjct: 141 LTSLG-SCSGLKFLNVSSNTLDFPGKVSGGLKLNSLEVLDLSANSISGANVV 191
>At3g54240.1 68416.m05995 hydrolase, alpha/beta fold family protein
low similarity to SP|P22862|ESTE_PSEFL Arylesterase (EC
3.1.1.2) (Aryl-ester hydrolase) {Pseudomonas
fluorescens}; contains Pfam profile PF00561: hydrolase,
alpha/beta fold family
Length = 350
Score = 27.9 bits (59), Expect = 5.4
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = -3
Query: 216 ILDFMPRSAAGANSVSAPRNISWPSLRERKTW 121
+L ++P AGA + N WPS + TW
Sbjct: 155 VLKYIPHRLAGATLLCPVTNSWWPSFPDSLTW 186
>At3g44340.1 68416.m04764 sec23/sec24 transport family protein
contains Pfam domains PF04811: Sec23/Sec24 trunk domain,
PF04815: Sec23/Sec24 helical domain and PF04810:
Sec23/Sec24 zinc finger
Length = 1096
Score = 27.9 bits (59), Expect = 5.4
Identities = 16/71 (22%), Positives = 31/71 (43%)
Frame = +2
Query: 122 QVLRSLKEGQEMFLGADTLLAPAALLGIKSSIGTSFNLFPKLARDILDAIENNDIAKARA 301
QVL L EG F+G T + +K ++ L +D+ +E + I +
Sbjct: 535 QVLSDLPEGPRTFVGIATFDSTIHFYNLKRALQQPLMLIVPDVQDVYTPLETDVIVQLSE 594
Query: 302 LQEKLSLAIEA 334
++ L + +E+
Sbjct: 595 CRQHLEILLES 605
>At2g26340.1 68415.m03160 expressed protein
Length = 230
Score = 27.9 bits (59), Expect = 5.4
Identities = 24/92 (26%), Positives = 44/92 (47%)
Frame = +2
Query: 56 ATARISNFKGLKFTSNDLNEGAQVLRSLKEGQEMFLGADTLLAPAALLGIKSSIGTSFNL 235
A A+ ++ G T+ D + + LR+L+E +FL A + A ++ +KS +GT+
Sbjct: 125 AVAQYASDDGFSKTATD--DVDRCLRALEELDSLFLRASRKDSNATVVLMKSQLGTALTA 182
Query: 236 FPKLARDILDAIENNDIAKARALQEKLSLAIE 331
L L + + + K +A+ E A E
Sbjct: 183 LDSL----LQTVPSQVLDKGKAMVEVYRSASE 210
>At4g32640.1 68417.m04646 sec23/sec24 transport protein-related
Length = 1069
Score = 27.5 bits (58), Expect = 7.2
Identities = 17/80 (21%), Positives = 34/80 (42%)
Frame = +2
Query: 95 TSNDLNEGAQVLRSLKEGQEMFLGADTLLAPAALLGIKSSIGTSFNLFPKLARDILDAIE 274
T+ N QVL L EG F+G T + +K ++ L +D+ +E
Sbjct: 523 TAAACNAIQQVLSDLPEGPRTFVGIATFDSTIHFYNLKRALQQPLMLIVPDVQDVYTPLE 582
Query: 275 NNDIAKARALQEKLSLAIEA 334
+ + + ++ L L +++
Sbjct: 583 TDVVVQLSECRQHLELLLDS 602
>At3g07430.1 68416.m00886 YGGT family protein contains Pfam profile
PF02325: YGGT family (unknown function)
Length = 232
Score = 27.5 bits (58), Expect = 7.2
Identities = 18/57 (31%), Positives = 27/57 (47%), Gaps = 3/57 (5%)
Frame = +2
Query: 284 IAKARALQEKLSLAIEAHT---AEGAWVPIMKAGMEIATGIKVGPPSLPQRPISAEA 445
IA R L +KLSLAI+ + A+G + AG ++ PP P++ A
Sbjct: 95 IAVTRVLAQKLSLAIQTSSPVIADGLRFSLSTAGPVFFASLRDRPPGYLNTPLTVVA 151
>At2g05990.2 68415.m00652 enoyl-[acyl-carrier protein] reductase
[NADH], chloroplast, putative / NADH-dependent enoyl-ACP
reductase, putative strong similarity to
enoyl-[acyl-carrier protein] reductase [NADH] SP:P80030
from [Brassica napus]
Length = 390
Score = 27.5 bits (58), Expect = 7.2
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -3
Query: 126 TWAPSLRSFEVNLRPLKFD 70
TW P+L FE +LR KFD
Sbjct: 127 TWVPALNIFETSLRRGKFD 145
>At2g05990.1 68415.m00651 enoyl-[acyl-carrier protein] reductase
[NADH], chloroplast, putative / NADH-dependent enoyl-ACP
reductase, putative strong similarity to
enoyl-[acyl-carrier protein] reductase [NADH] SP:P80030
from [Brassica napus]
Length = 390
Score = 27.5 bits (58), Expect = 7.2
Identities = 11/19 (57%), Positives = 13/19 (68%)
Frame = -3
Query: 126 TWAPSLRSFEVNLRPLKFD 70
TW P+L FE +LR KFD
Sbjct: 127 TWVPALNIFETSLRRGKFD 145
>At1g54000.1 68414.m06152 myrosinase-associated protein, putative
similar to myrosinase-associated proteins GI:1769968,
GI:1769970, GI:1216391, GI:1216389 from [Brassica
napus]; contains Pfam profile PF00657: GDSL-like
Lipase/Acylhydrolase; contains 1 predicted transmembrane
domain
Length = 391
Score = 27.5 bits (58), Expect = 7.2
Identities = 9/22 (40%), Positives = 14/22 (63%)
Frame = -1
Query: 593 PIQTPLRPYSFPPTSITLHLTD 528
P+ P+R + PPT T+H +D
Sbjct: 358 PVDEPMREFWVPPTPATVHASD 379
>At5g15740.1 68418.m01841 expressed protein contains Pfam PF03138:
Plant protein family. The function of this family of
plant proteins is unknown;
Length = 508
Score = 27.1 bits (57), Expect = 9.5
Identities = 14/37 (37%), Positives = 22/37 (59%)
Frame = +2
Query: 35 MPAFVKEATARISNFKGLKFTSNDLNEGAQVLRSLKE 145
+P V++ R+ NF GLKFT G +V++ L+E
Sbjct: 234 LPVEVQKLRCRV-NFNGLKFTPQIEELGRRVVKILRE 269
>At4g29380.1 68417.m04197 protein kinase family protein / WD-40 repeat
family protein contains Pfam PF00400: WD domain, G-beta
repeat; contains Pfam PF00069: Protein kinase domain;
contains PF02985: HEAT repeat; similar to adaptor protein
(GI:1817584) [Homo sapiens]; similar to VPS15 protein
(GI:6103009) [Pichia pastoris]
Length = 1494
Score = 27.1 bits (57), Expect = 9.5
Identities = 20/55 (36%), Positives = 26/55 (47%)
Frame = -3
Query: 315 SFSCKALALAMSLFSIASSISRASLGNRLNEVPILDFMPRSAAGANSVSAPRNIS 151
SFS A + SS+ SLG VP +D M +S A+SV P+ IS
Sbjct: 938 SFSMDKRAATNPPVASESSLQMNSLGMGSLSVPWMDSMSKSFNLASSVPVPKLIS 992
>At3g48110.2 68416.m05246 aminoacyl-t-RNA synthetase, putative
similar to aminoacyl-t-RNA synthetase GI:2654226 from
[Arabidopsis thaliana]; contains Pfam profiles: PF02092
glycyl-tRNA synthetase beta subunit,PF02091 glycyl-tRNA
synthetase alpha subunit
Length = 1063
Score = 27.1 bits (57), Expect = 9.5
Identities = 11/23 (47%), Positives = 13/23 (56%)
Frame = +1
Query: 505 EALREYWRSVKCNVMDVGGKEYG 573
+ L+EYW SV C VM E G
Sbjct: 78 QRLQEYWASVGCAVMQPSNTEVG 100
>At3g48110.1 68416.m05245 aminoacyl-t-RNA synthetase, putative
similar to aminoacyl-t-RNA synthetase GI:2654226 from
[Arabidopsis thaliana]; contains Pfam profiles: PF02092
glycyl-tRNA synthetase beta subunit,PF02091 glycyl-tRNA
synthetase alpha subunit
Length = 1064
Score = 27.1 bits (57), Expect = 9.5
Identities = 11/23 (47%), Positives = 13/23 (56%)
Frame = +1
Query: 505 EALREYWRSVKCNVMDVGGKEYG 573
+ L+EYW SV C VM E G
Sbjct: 78 QRLQEYWASVGCAVMQPSNTEVG 100
>At3g44330.1 68416.m04763 expressed protein
Length = 565
Score = 27.1 bits (57), Expect = 9.5
Identities = 11/25 (44%), Positives = 16/25 (64%)
Frame = -3
Query: 177 SVSAPRNISWPSLRERKTWAPSLRS 103
SVS PR I+ P++ + W P LR+
Sbjct: 192 SVSEPRKIASPTITNIQGWLPGLRA 216
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,269,209
Number of Sequences: 28952
Number of extensions: 267306
Number of successful extensions: 810
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 792
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 810
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1190791976
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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