Samia BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_E01
         (372 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein L20|Sc...    51   5e-08
SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosom...    51   5e-08
SPBC8D2.05c |sfi1||spindle pole body protein Sfi1|Schizosaccharo...    25   5.0  
SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces pombe...    24   8.8  
SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual    24   8.8  

>SPAC26A3.04 |rpl2002|rpl20, rpl20-2|60S ribosomal protein
           L20|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 176

 Score = 51.2 bits (117), Expect = 5e-08
 Identities = 29/73 (39%), Positives = 38/73 (52%)
 Frame = +3

Query: 150 VKKTTGVKLVSSRGNSRKESC*D*NFGIWLRYESRSGVHNMYREYRELEW*VCSHQCYRD 329
           VKK TG ++V+    S  +      FGIW+RY+SRSG HNMY+E+R+          Y D
Sbjct: 50  VKKATG-EIVAINEISEPKPLKAKVFGIWIRYDSRSGTHNMYKEFRDTTRVGAVEAMYAD 108

Query: 330 MGXXXXXXXXSIR 368
           M         SIR
Sbjct: 109 MAARHRARFRSIR 121



 Score = 29.1 bits (62), Expect = 0.23
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = +2

Query: 86  FLSDPIVAKSRFWYFLRQLXERQK 157
           F  +  VAKSR+WYFL+ + + +K
Sbjct: 29  FAPNESVAKSRYWYFLKMINKVKK 52


>SPAC3A12.10 |rpl2001|rpl20-1, rpl20, yl17b, rpl18a-2|60S ribosomal
           protein L20a|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 176

 Score = 51.2 bits (117), Expect = 5e-08
 Identities = 29/73 (39%), Positives = 38/73 (52%)
 Frame = +3

Query: 150 VKKTTGVKLVSSRGNSRKESC*D*NFGIWLRYESRSGVHNMYREYRELEW*VCSHQCYRD 329
           VKK TG ++V+    S  +      FGIW+RY+SRSG HNMY+E+R+          Y D
Sbjct: 50  VKKATG-EIVAINEISEPKPLKAKVFGIWIRYDSRSGTHNMYKEFRDTTRVGAVEAMYAD 108

Query: 330 MGXXXXXXXXSIR 368
           M         SIR
Sbjct: 109 MAARHRARFRSIR 121



 Score = 29.1 bits (62), Expect = 0.23
 Identities = 11/24 (45%), Positives = 17/24 (70%)
 Frame = +2

Query: 86  FLSDPIVAKSRFWYFLRQLXERQK 157
           F  +  VAKSR+WYFL+ + + +K
Sbjct: 29  FAPNESVAKSRYWYFLKMINKVKK 52


>SPBC8D2.05c |sfi1||spindle pole body protein
           Sfi1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 840

 Score = 24.6 bits (51), Expect = 5.0
 Identities = 16/65 (24%), Positives = 30/65 (46%), Gaps = 5/65 (7%)
 Frame = +2

Query: 95  DPIVAKSRFWYFLRQL--XERQKDY---WXEIGILKGKFQKRVLLRLELWYLVALRVSLW 259
           D ++   RFWY+L +    + ++D+          K  +Q+R+ L+ E   L   R+   
Sbjct: 476 DRLIVTFRFWYYLMKAKSFQHKRDHRLLTIAWEAWKDNYQRRIKLQKEAQELQLTRIQKI 535

Query: 260 CPQYV 274
             QY+
Sbjct: 536 AKQYL 540


>SPBC8D2.18c |||adenosylhomocysteinase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 433

 Score = 23.8 bits (49), Expect = 8.8
 Identities = 7/15 (46%), Positives = 13/15 (86%)
 Frame = +3

Query: 246 ESRSGVHNMYREYRE 290
           E+ +GVHN+Y+ ++E
Sbjct: 157 ETTTGVHNLYKMFKE 171


>SPAC630.13c |tsc2||tuberin|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1339

 Score = 23.8 bits (49), Expect = 8.8
 Identities = 15/42 (35%), Positives = 21/42 (50%)
 Frame = -2

Query: 323 IALMTAHLPLKFTVFTVHIVDTRARLVTQPDTKVLILTGLFS 198
           + L    LP+        I+ T +RL+T+PD  V IL  L S
Sbjct: 812 LMLCCYELPVSIRKQLPAILVTLSRLITKPDLSVHILEFLCS 853


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,449,764
Number of Sequences: 5004
Number of extensions: 26717
Number of successful extensions: 65
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 62
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 65
length of database: 2,362,478
effective HSP length: 65
effective length of database: 2,037,218
effective search space used: 118158644
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)