Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_D18
(323 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family pr... 61 2e-10
At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolas... 57 2e-09
At5g43280.1 68418.m05290 enoyl-CoA hydratase/isomerase family pr... 52 1e-07
At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolas... 50 5e-07
At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolas... 49 8e-07
At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family pr... 46 4e-06
At3g60510.1 68416.m06768 enoyl-CoA hydratase/isomerase family pr... 46 6e-06
At3g24360.1 68416.m03058 enoyl-CoA hydratase/isomerase family pr... 43 5e-05
At4g13360.1 68417.m02089 enoyl-CoA hydratase/isomerase family pr... 41 2e-04
At1g60550.1 68414.m06816 naphthoate synthase, putative / dihydro... 40 3e-04
At1g06550.1 68414.m00694 enoyl-CoA hydratase/isomerase family pr... 40 4e-04
At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fat... 33 0.033
At4g29050.1 68417.m04155 lectin protein kinase family protein co... 30 0.41
At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase f... 29 0.94
At1g12820.1 68414.m01489 transport inhibitor response protein, p... 27 2.9
At2g46740.1 68415.m05832 FAD-binding domain-containing protein s... 27 3.8
At1g17360.1 68414.m02116 COP1-interacting protein-related simila... 27 3.8
At5g52100.1 68418.m06467 dihydrodipicolinate reductase family pr... 26 5.0
At3g28360.1 68416.m03544 ABC transporter family protein similar ... 26 6.7
At1g64670.1 68414.m07331 hydrolase, alpha/beta fold family prote... 25 8.8
>At4g16210.1 68417.m02460 enoyl-CoA hydratase/isomerase family
protein similar to 3-hydroxybutyryl-CoA dehydratase
(Crotonase) from Clostridium acetobutylicum [SP|P52046],
FadB1x (enoyl-CoA hydratase) from Pseudomonas putida
[GI:13310130]; contains Pfam profile PF00378 enoyl-CoA
hydratase/isomerase family protein
Length = 265
Score = 60.9 bits (141), Expect = 2e-10
Identities = 26/74 (35%), Positives = 47/74 (63%)
Frame = +1
Query: 88 VYENIKIDVVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGNE 267
V EN+ I V + +I +NRPK+LN+L + + V+L KA D D+D ++ ++ TG+
Sbjct: 5 VSENL-IQVKKESGGIAVITINRPKSLNSLTRAMMVDLAKAFKDMDSDESVQVVIFTGSG 63
Query: 268 KAFAAGADITEMQN 309
++F +G D+T ++
Sbjct: 64 RSFCSGVDLTAAES 77
>At2g30660.1 68415.m03739 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 378
Score = 57.2 bits (132), Expect = 2e-09
Identities = 25/70 (35%), Positives = 41/70 (58%)
Frame = +1
Query: 112 VVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGNEKAFAAGAD 291
+V K +V ++ LNRPK LNALC + L + ++ DP++ ++L G +AF AG D
Sbjct: 8 LVEEKSSVRILTLNRPKQLNALCFNMISRLLQLFRAYEEDPSVKLVILKGQGRAFCAGGD 67
Query: 292 ITEMQNNTIQ 321
+ + N +Q
Sbjct: 68 VPPVVQNMVQ 77
>At5g43280.1 68418.m05290 enoyl-CoA hydratase/isomerase family
protein similar to Delta 3,5-delta2,4-dienoyl-CoA
isomerase, mitochondrial (ECH1) from Rattus norvegicus
[SP|Q62651], from Homo sapiens [SP|Q13011]; contains
Pfam profile PF00378 enoyl-CoA hydratase/isomerase
family protein
Length = 278
Score = 51.6 bits (118), Expect = 1e-07
Identities = 24/77 (31%), Positives = 44/77 (57%)
Frame = +1
Query: 91 YENIKIDVVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGNEK 270
Y+ ++I + +V + +NRP LNAL F+E KA++ D +P ++ I+L+G K
Sbjct: 6 YKTLEIIRKNTDSSVFHLIINRPSHLNALSLDFFIEFPKALSSLDQNPDVSVIILSGAGK 65
Query: 271 AFAAGADITEMQNNTIQ 321
F +G D+ + + + Q
Sbjct: 66 HFCSGIDLNSLSSISTQ 82
>At5g65940.1 68418.m08301 3-hydroxyisobutyryl-coenzyme A hydrolase /
CoA-thioester hydrolase (CHY1) identical to gi:8572760;
contains Pfam profile PF00388 enoyl-CoA
hydratase/isomerase family protein
Length = 378
Score = 49.6 bits (113), Expect = 5e-07
Identities = 23/61 (37%), Positives = 37/61 (60%)
Frame = +1
Query: 112 VVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGNEKAFAAGAD 291
+V K +V ++ LNRPK LNAL + L + F+ DP++ ++L G+ +AF AG D
Sbjct: 12 LVEEKSSVRILTLNRPKQLNALSFHMISRLLQLFLAFEEDPSVKLVILKGHGRAFCAGGD 71
Query: 292 I 294
+
Sbjct: 72 V 72
>At2g30650.1 68415.m03738 3-hydroxyisobutyryl-coenzyme A hydrolase,
putative / CoA-thioester hydrolase, putative strong
similarity to gi:8572760; contains Pfam profile PF00388
enoyl-CoA hydratase/isomerase family protein
Length = 422
Score = 48.8 bits (111), Expect = 8e-07
Identities = 24/70 (34%), Positives = 40/70 (57%)
Frame = +1
Query: 112 VVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGNEKAFAAGAD 291
+V K +V ++ NRPK LNAL + L + ++ DP++ +VL G +AF+AG D
Sbjct: 52 LVEEKSSVRILTFNRPKQLNALSFHMVSRLLQLFLAYEEDPSVKLVVLKGQGRAFSAGGD 111
Query: 292 ITEMQNNTIQ 321
I + + +Q
Sbjct: 112 IPPIVRDILQ 121
>At4g31810.1 68417.m04521 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 409
Score = 46.4 bits (105), Expect = 4e-06
Identities = 24/80 (30%), Positives = 44/80 (55%)
Frame = +1
Query: 73 YSAAPVYENIKIDVVGSKKNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIV 252
YSA+ ++ V G K+ I LN P +LNAL P+ L + ++ +P I+ ++
Sbjct: 32 YSASDADFEDQVLVEGKAKSRAAI-LNNPSSLNALSAPMVGRLKRLYESWEENPAISFVL 90
Query: 253 LTGNEKAFAAGADITEMQNN 312
+ G+ K F +GAD+ + ++
Sbjct: 91 MKGSGKTFCSGADVLSLYHS 110
>At3g60510.1 68416.m06768 enoyl-CoA hydratase/isomerase family
protein similar to enoyl-CoA-hydratase, Avicennia
marina, EMBL:AF190450 [GI:6014701], CoA-thioester
hydrolase CHY1 from Arabidopsis thaliana [GI:8572760];
contains Pfam profile PF00378 enoyl-CoA
hydratase/isomerase family protein
Length = 401
Score = 46.0 bits (104), Expect = 6e-06
Identities = 21/52 (40%), Positives = 31/52 (59%)
Frame = +1
Query: 148 LNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGNEKAFAAGADITEM 303
LNRP ALNAL + L K +++ DP I +++ G+ +AF AG DI +
Sbjct: 52 LNRPPALNALTTHMGYRLQKLYKNWEEDPNIGFVMMKGSGRAFCAGGDIVSL 103
>At3g24360.1 68416.m03058 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 418
Score = 42.7 bits (96), Expect = 5e-05
Identities = 21/55 (38%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
Frame = +1
Query: 133 VGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGN-EKAFAAGADI 294
V LI L+RPKALNA+ + ++ +++++ DP + +V+ G+ +AF AG DI
Sbjct: 56 VALITLDRPKALNAMNLEMDLKYKSLLDEWEYDPGVKCVVVEGSTSRAFCAGMDI 110
>At4g13360.1 68417.m02089 enoyl-CoA hydratase/isomerase family
protein similar to CoA-thioester hydrolase CHY1
(beta-hydroxyisobutyryl-CoA hydrolase) [Arabidopsis
thaliana] GI:8572760; contains Pfam profile PF00378:
enoyl-CoA hydratase/isomerase family protein
Length = 381
Score = 41.1 bits (92), Expect = 2e-04
Identities = 19/55 (34%), Positives = 36/55 (65%), Gaps = 1/55 (1%)
Frame = +1
Query: 133 VGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGN-EKAFAAGADI 294
V LI L+R KALNA+ + ++ ++++++DP + +++ G+ +AF AG DI
Sbjct: 19 VALITLDRTKALNAMNLDMDIKYKSFLDEWESDPRVKCVIVEGSTSRAFCAGMDI 73
>At1g60550.1 68414.m06816 naphthoate synthase, putative /
dihydroxynaphthoic acid synthetase, putative / DHNA
synthetase, putative contains similarity to MENB from
Escherichia coli [SP|P27290], Bacillus subtilis
[SP|P23966]; contains Pfam profile PF00378 enoyl-CoA
hydratase/isomerase family protein
Length = 337
Score = 40.3 bits (90), Expect = 3e-04
Identities = 23/66 (34%), Positives = 35/66 (53%), Gaps = 4/66 (6%)
Frame = +1
Query: 106 IDVVGSK---KNVGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTG-NEKA 273
+D++ K + + I +NRP+ NA EL +A ND D ++ I+LTG KA
Sbjct: 75 VDIIYEKALDEGIAKITINRPERRNAFRPQTVKELMRAFNDARDDSSVGVIILTGKGTKA 134
Query: 274 FAAGAD 291
F +G D
Sbjct: 135 FCSGGD 140
>At1g06550.1 68414.m00694 enoyl-CoA hydratase/isomerase family
protein similar to CHY1 [gi:8572760]; contains Pfam
profile PF00388 enoyl-CoA hydratase/isomerase family
protein
Length = 387
Score = 39.9 bits (89), Expect = 4e-04
Identities = 20/62 (32%), Positives = 36/62 (58%), Gaps = 1/62 (1%)
Frame = +1
Query: 112 VVGSKK-NVGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGNEKAFAAGA 288
V+G +K +V L LNRP+ LN + + +L + + ++ D I++ G +AF+AG
Sbjct: 13 VIGEEKGSVRLTTLNRPRQLNVISPEVVFKLAEYLELWEKDDQTKLILIKGTGRAFSAGG 72
Query: 289 DI 294
D+
Sbjct: 73 DL 74
>At4g29010.1 68417.m04147 abnormal inflorescence meristem 1 / fatty
acid multifunctional protein (AIM1) identical to
gi:4337025; contains Pfam profiles PF02737
(3-hydroxyacyl-CoA dehydrogenase, NAD binding domain),
PF00378 (enoyl-CoA hydratase/isomerase family protein),
PF00725 (3-hydroxyacyl-CoA dehydrogenase)
Length = 721
Score = 33.5 bits (73), Expect = 0.033
Identities = 19/58 (32%), Positives = 29/58 (50%)
Frame = +1
Query: 133 VGLIQLNRPKALNALCKPLFVELGKAVNDFDADPTIAAIVLTGNEKAFAAGADITEMQ 306
V +I ++ P +N+L P+ L + D + + AIVL GN F+ G DI Q
Sbjct: 16 VAVITISNPP-VNSLASPIISGLKEKFRDANQRNDVKAIVLIGNNGRFSGGFDINVFQ 72
>At4g29050.1 68417.m04155 lectin protein kinase family protein
contains Pfam domains, PF00138: Legume lectins alpha
domain, PF00139: Legume lectins beta domain and PF00069:
Protein kinase domain
Length = 669
Score = 29.9 bits (64), Expect = 0.41
Identities = 19/61 (31%), Positives = 33/61 (54%), Gaps = 2/61 (3%)
Frame = +1
Query: 19 LFCNTKIAAV--SNTVLVKFYSAAPVYENIKIDVVGSKKNVGLIQLNRPKALNALCKPLF 192
LFC+ +AAV S + +++F N D+V +++NVG I+ +A +L +P
Sbjct: 596 LFCSHPVAAVRPSMSSVIQFLDGVAQLPNNLFDIVKARENVGAIE-GFGEAAESLAEPCS 654
Query: 193 V 195
V
Sbjct: 655 V 655
>At3g55360.1 68416.m06148 3-oxo-5-alpha-steroid 4-dehydrogenase
family protein / steroid 5-alpha-reductase family
protein similar to synaptic glycoprotein SC2 spliced
variant from Homo sapiens [EMBL:AF038958], SC2 from
Rattus sp. [gi:256994]; contains Pfam
3-oxo-5-alpha-steroid 4-dehydrogenase domain PF02544
Length = 310
Score = 28.7 bits (61), Expect = 0.94
Identities = 21/66 (31%), Positives = 30/66 (45%), Gaps = 3/66 (4%)
Frame = -1
Query: 284 PAAKAFSLPV---STIAAMVGSASKSLTALPSSTNSGLQRAFSALGRFSCIRPTFFFEPT 114
P+ + +LPV S +V ++ KSL N+ L F LG R FFFE
Sbjct: 42 PSRQRLTLPVTPGSKDKPVVLNSKKSLKEYCDGNNNSLTVVFKDLGAQVSYRTLFFFEYL 101
Query: 113 TSILIF 96
+LI+
Sbjct: 102 GPLLIY 107
>At1g12820.1 68414.m01489 transport inhibitor response protein,
putative E3 ubiquitin ligase SCF complex F-box subunit;
similar to transport inhibitor response 1 GI:2352492
from [Arabidopsis thaliana]
Length = 577
Score = 27.1 bits (57), Expect = 2.9
Identities = 18/58 (31%), Positives = 29/58 (50%), Gaps = 5/58 (8%)
Frame = +1
Query: 112 VVGSKKNVGLIQLNRPKALNAL-----CKPLFVELGKAVNDFDADPTIAAIVLTGNEK 270
+V N+ ++LNR L+AL C P V+LG + + DP A ++T +K
Sbjct: 201 LVARSPNLKSLKLNRAVPLDALARLMSCAPQLVDLGVGSYENEPDPESFAKLMTAIKK 258
>At2g46740.1 68415.m05832 FAD-binding domain-containing protein
strong similarity to At1g32300, At5g56490, At2g46750,
At2g46760; contains PF01565: FAD binding domain
Length = 590
Score = 26.6 bits (56), Expect = 3.8
Identities = 14/55 (25%), Positives = 28/55 (50%)
Frame = -1
Query: 269 FSLPVSTIAAMVGSASKSLTALPSSTNSGLQRAFSALGRFSCIRPTFFFEPTTSI 105
FS+P++ + + + KSL + S + GL+ + L R+ P + + T +I
Sbjct: 403 FSIPLTQVKSFINDI-KSLLKIDSKSLCGLELYYGILMRYVTSSPAYLGKETEAI 456
>At1g17360.1 68414.m02116 COP1-interacting protein-related similar
to COP1-Interacting Protein 7 (CIP7) (GI:3327870)
[Arabidopsis thaliana]
Length = 1032
Score = 26.6 bits (56), Expect = 3.8
Identities = 16/37 (43%), Positives = 21/37 (56%)
Frame = -1
Query: 275 KAFSLPVSTIAAMVGSASKSLTALPSSTNSGLQRAFS 165
+A +LP ST+ V S S + P S +S LQ AFS
Sbjct: 875 QANTLPSSTLQHNVASLFDSPSESPLSWSSNLQHAFS 911
>At5g52100.1 68418.m06467 dihydrodipicolinate reductase family
protein weak similarity to dihydrodipicolinate reductase
[Corynebacterium glutamicum] GI:311768; contains Pfam
profiles PF01113: Dihydrodipicolinate reductase
N-terminus, PF05173: Dihydrodipicolinate reductase
C-terminus
Length = 298
Score = 26.2 bits (55), Expect = 5.0
Identities = 15/44 (34%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
Frame = +1
Query: 58 VLVKFYSAAPVYENIKIDVVGSKKNVGLIQLNRPK---ALNALC 180
V++ F + VYEN+K K+V + +P+ AL+ALC
Sbjct: 107 VVIDFTDPSTVYENVKQATAFGMKSVVYVPRIKPETVSALSALC 150
>At3g28360.1 68416.m03544 ABC transporter family protein similar to
P-glycoprotein homologue GI:2292907 from [Hordeum
vulgare subsp. vulgare]
Length = 1158
Score = 25.8 bits (54), Expect = 6.7
Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 3/47 (6%)
Frame = +1
Query: 139 LIQLNRPKALNALCKPLFVELGKAVND---FDADPTIAAIVLTGNEK 270
L+ +NRP+ +ALC L LG AV + + I+ LT +E+
Sbjct: 577 LMAMNRPEWKHALCGCLSASLGGAVQPIYAYSSGLMISVFFLTNHEQ 623
>At1g64670.1 68414.m07331 hydrolase, alpha/beta fold family protein
low similarity to
2-hydroxy-6-oxo-7-methylocta-2,4-dienoate hydrolase
[Pseudomonas putida] GI:2822275; contains Pfam profile
PF00561: hydrolase, alpha/beta fold family
Length = 469
Score = 25.4 bits (53), Expect = 8.8
Identities = 17/51 (33%), Positives = 23/51 (45%), Gaps = 4/51 (7%)
Frame = +3
Query: 120 FKK---KCRPYTAESPQSTERSLQTTVCGAR*SSQRL-RCRSNHCRYCTDW 260
FKK KC + + ST+++L T QR R HC +CT W
Sbjct: 111 FKKDVVKCCDESKKRSPSTKKTLLTVNSTVVEKLQRTPRWSDCHCTFCTSW 161
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,189,017
Number of Sequences: 28952
Number of extensions: 136990
Number of successful extensions: 366
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 361
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 366
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 360538848
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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