Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_D08
(566 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 264 5e-72
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 141 7e-35
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 139 3e-34
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 134 1e-32
SPBC354.13 |rga6||GTPase activating protein Rga6|Schizosaccharom... 28 1.1
SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual 27 1.5
SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis Mei2... 27 1.9
SPBC19G7.16 |iws1||transcription elongation factor complex subun... 27 1.9
SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein Pof11|Schizos... 27 2.5
SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase Lkh1|Schi... 26 4.4
SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1 |Schizos... 25 5.9
SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1 |Schiz... 25 5.9
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 264 bits (648), Expect = 5e-72
Identities = 116/172 (67%), Positives = 136/172 (79%)
Frame = +3
Query: 51 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYV 230
MREIVHIQAGQCGNQ+GA FW I+DEHG+D G YHG S+ Q ER+NVY+NEA+GGKYV
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGGKYV 60
Query: 231 PRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVV 410
PRA+LVDLEPGTMD+V+SG FG +FRPDN ++GQSGAGN WAKGHYTEGAEL D+VLDVV
Sbjct: 61 PRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVV 120
Query: 411 RKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPS 566
R+E+E+CD LQGFQ KIREEYPDR+M T+SV P+
Sbjct: 121 RREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPA 172
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 141 bits (341), Expect = 7e-35
Identities = 60/173 (34%), Positives = 105/173 (60%), Gaps = 2/173 (1%)
Frame = +3
Query: 54 REIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERINVYYNEASGGKYVP 233
REI+ +QAGQCGNQIG++FW+ + EHGI P G + ++R +V++ ++ +Y+P
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFATEGVDRKDVFFYQSDDTRYIP 62
Query: 234 RAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQS--GAGNNWAKGHYTEGAELVDSVLDV 407
RAIL+DLEP ++++ S +G ++ P+N + ++ GAGNNWA G Y+ + + ++D+
Sbjct: 63 RAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDM 121
Query: 408 VRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPS 566
+ +E++ D L+GF ++ + YP +I+ TYSV P+
Sbjct: 122 IDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPN 174
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 139 bits (336), Expect = 3e-34
Identities = 67/174 (38%), Positives = 96/174 (55%), Gaps = 2/174 (1%)
Frame = +3
Query: 51 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTGAYHGDSDLQLER--INVYYNEASGGK 224
MREI+ I GQ G QIG WE+ EHGI P G + ++ Q + +++E GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 225 YVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLD 404
YVPR+I VDLEP +D VR+GP+ +F P+ + G+ A NN+A+GHYT G ELVD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 405 VVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPS 566
+R+ +++C LQGF ++ EY + +SV P+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPA 174
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 134 bits (323), Expect = 1e-32
Identities = 64/178 (35%), Positives = 97/178 (54%), Gaps = 6/178 (3%)
Frame = +3
Query: 51 MREIVHIQAGQCGNQIGAKFWEIISDEHGIDPTG------AYHGDSDLQLERINVYYNEA 212
MRE++ + GQ G QIG WE+ EHGI P G H ++ + +++E
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60
Query: 213 SGGKYVPRAILVDLEPGTMDSVRSGPFGQIFRPDNFVFGQSGAGNNWAKGHYTEGAELVD 392
GK+VPR+I VDLEP +D VR+GP+ +F P+ V G+ A NN+A+GHYT G E++D
Sbjct: 61 GQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMID 120
Query: 393 SVLDVVRKESESCDCLQGFQXXXXXXXXXXXXXXXXXXXKIREEYPDRIMNTYSVVPS 566
SVL+ +R+ +++C LQGF ++ EY + +SV P+
Sbjct: 121 SVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPA 178
>SPBC354.13 |rga6||GTPase activating protein
Rga6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 733
Score = 27.9 bits (59), Expect = 1.1
Identities = 16/36 (44%), Positives = 25/36 (69%), Gaps = 1/36 (2%)
Frame = +1
Query: 460 TLSE-AVPAPVWAHYLSQRSVKSTPTES*THTQSSP 564
T+S+ AVP+ +A+ +S RSV S T+S T++SP
Sbjct: 643 TVSDTAVPSMSFANNISSRSVISAATDSKPSTRTSP 678
>SPAC6C3.08 |||gankyrin|Schizosaccharomyces pombe|chr 1|||Manual
Length = 234
Score = 27.5 bits (58), Expect = 1.5
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 354 AKGHYTEGAELVDSVLDVVRKESESCDCLQ 443
A+GH G ELV + D +RK+SE+ L+
Sbjct: 183 AEGHPDVGVELVRAGADTLRKDSENHTALE 212
>SPAC27D7.03c |mei2||RNA-binding protein involved in meiosis
Mei2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 750
Score = 27.1 bits (57), Expect = 1.9
Identities = 21/80 (26%), Positives = 38/80 (47%), Gaps = 1/80 (1%)
Frame = +1
Query: 292 SDRYSALTTLFSDSPAPVTTGPRDTTQRALS*STQFLT*SEK-NPNHVIVYKDSNSHTLS 468
++ Y +TL P+P+ T+ + S S +LT ++ PN V + N++ S
Sbjct: 45 NEHYPPKSTLLLSGPSPIRNIQLSATKSSESNSIDYLTDTQNIFPNFV---NNENNYQFS 101
Query: 469 EAVPAPVWAHYLSQRSVKST 528
A P+ A + +R V +T
Sbjct: 102 TAPLNPIDACRVGERKVFTT 121
>SPBC19G7.16 |iws1||transcription elongation factor complex subunit
Iws1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 428
Score = 27.1 bits (57), Expect = 1.9
Identities = 10/34 (29%), Positives = 22/34 (64%)
Frame = +3
Query: 90 NQIGAKFWEIISDEHGIDPTGAYHGDSDLQLERI 191
N++G E+++++ +DPT A + DLQ++ +
Sbjct: 133 NELGENEEEVLTEQKQLDPTLAAKKELDLQMDAV 166
>SPAC29E6.01 |pof11|SPAC30.05, mug156|F-box protein
Pof11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 506
Score = 26.6 bits (56), Expect = 2.5
Identities = 14/35 (40%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = +3
Query: 285 GPFGQIFRPDNFVFGQSGAG-NNWAKGHYTEGAEL 386
GP+G +F P F+F +G NW+ Y E A L
Sbjct: 157 GPYGTMFLPQQFIFDSNGRPLLNWSY-LYKEHAHL 190
>SPAC1D4.11c |lkh1|kic1|dual specificity protein kinase
Lkh1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 690
Score = 25.8 bits (54), Expect = 4.4
Identities = 14/40 (35%), Positives = 18/40 (45%)
Frame = +1
Query: 421 PNHVIVYKDSNSHTLSEAVPAPVWAHYLSQRSVKSTPTES 540
P VIV +DS S L+ +P P H L P+ S
Sbjct: 23 PQEVIVIEDSASPRLTPNLPPPFSVHQLQSFVPPQPPSSS 62
>SPAC23A1.19c ||SPAC26H5.01c|RecQ type DNA helicase Hrq1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1063
Score = 25.4 bits (53), Expect = 5.9
Identities = 12/44 (27%), Positives = 20/44 (45%)
Frame = +3
Query: 306 RPDNFVFGQSGAGNNWAKGHYTEGAELVDSVLDVVRKESESCDC 437
RP +F G++ G + E D ++ + + ESCDC
Sbjct: 955 RPSRLIF-YDNCGDSSGAGLCNKAYEHTDELITMAIERIESCDC 997
>SPAC8F11.09c |nnt1||nicotinamide N-methyltransferase Nnt1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 255
Score = 25.4 bits (53), Expect = 5.9
Identities = 13/32 (40%), Positives = 16/32 (50%)
Frame = -2
Query: 343 PAPDCPKTKLSGRNICPKGPERTESMVPGSRS 248
P+ PK L R I P GPE + + GS S
Sbjct: 20 PSTPPPKEVLHTRVIVPNGPEEIKLRLVGSHS 51
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,127,385
Number of Sequences: 5004
Number of extensions: 39816
Number of successful extensions: 115
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 110
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 111
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 240047038
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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