BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_D03
(504 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 106 7e-24
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 99 8e-22
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si... 97 4e-21
At4g16510.1 68417.m02498 YbaK/prolyl-tRNA synthetase-related con... 28 4.1
At1g79800.1 68414.m09316 plastocyanin-like domain-containing pro... 27 7.2
At1g47840.1 68414.m05325 hexokinase, putative similar to hexokin... 27 7.2
At5g36820.1 68418.m04411 F-box family protein contains Pfam PF00... 27 9.5
At5g36730.1 68418.m04395 F-box family protein contains Pfam PF00... 27 9.5
At1g18460.1 68414.m02303 lipase family protein similar to triacy... 27 9.5
>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
contains Pfam PF00728: Glycosyl hydrolase family 20,
catalytic domain; contains Pfam PF02838: Glycosyl
hydrolase family 20, domain 2; similar to
Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
(N-acetyl-beta- glucosaminidase)
(Beta-N-acetylhexosaminidase) (Hexosaminidase B)
(Swiss-Prot:P07686) [Homo sapiens]
Length = 535
Score = 106 bits (255), Expect = 7e-24
Identities = 61/142 (42%), Positives = 83/142 (58%), Gaps = 7/142 (4%)
Frame = +3
Query: 90 VQELHVELTAPCEKLPYFGMDESYNLXXXX-----XXXXXXXXIWGVIRGLETWSQL--F 248
+Q LHV +++ ++L Y G DESY L ++G + GL+T+SQL F
Sbjct: 102 LQGLHVIISSSTDELEY-GADESYKLVVPSPEKPSYAQLEAKSVYGALHGLQTFSQLCHF 160
Query: 249 YLTNDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLHWHI 428
L E+ + +I D PR+++RGLL+DTSRHYL L I ID+M K+NVLHWHI
Sbjct: 161 NLKKKVIEILMTPWNIIDQPRFSYRGLLIDTSRHYLPLPVIKNVIDSMTYAKLNVLHWHI 220
Query: 429 VDDQSFPYHSEKFPELSRLGAY 494
VD QSFP +P+L GAY
Sbjct: 221 VDTQSFPLEIPSYPKLWN-GAY 241
>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase precursor SP:P43077 from
[Candida albicans]
Length = 580
Score = 99 bits (238), Expect = 8e-22
Identities = 52/124 (41%), Positives = 70/124 (56%), Gaps = 3/124 (2%)
Frame = +3
Query: 138 YFGMDESYNLXX---XXXXXXXXXXIWGVIRGLETWSQLFYLTNDFRELRINSTDIYDYP 308
+ G+DESY L WG +RGLET+SQ+ + T+ L + I D P
Sbjct: 111 HHGVDESYKLSIPIGSFSAHLLAHSAWGAMRGLETFSQMIWGTSPDLCLPVGIY-IQDSP 169
Query: 309 RYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLG 488
+ HRG+LLDTSR+Y + I++ I AM+ANK+NV HWHI D QSFP P L+ G
Sbjct: 170 LFGHRGVLLDTSRNYYGVDDIMRTIKAMSANKLNVFHWHITDSQSFPLVLPSEPSLAAKG 229
Query: 489 AYHP 500
+ P
Sbjct: 230 SLGP 233
>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
similar to beta-hexosaminidase A SP:P13723 from
[Dictyostelium discoideum]
Length = 541
Score = 97.5 bits (232), Expect = 4e-21
Identities = 56/145 (38%), Positives = 82/145 (56%), Gaps = 10/145 (6%)
Frame = +3
Query: 90 VQELHVELTAPCEKLPYFGMDESYNLXXXX--------XXXXXXXXIWGVIRGLETWSQL 245
+ L + + + E+L G+DESY L ++G +RGLET+SQL
Sbjct: 102 ITSLKIVVHSDSEELQ-LGVDESYTLMVSKKNEQSIVGAATIEANTVYGALRGLETFSQL 160
Query: 246 FYLTNDFRELRINSTDIY--DYPRYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLH 419
+ ++I Y D PR+ +RGLL+DTSRHYL + I + I++M+ K+NVLH
Sbjct: 161 CAFDYITKSVQIYKAPWYIQDKPRFGYRGLLIDTSRHYLPIDVIKQIIESMSFAKLNVLH 220
Query: 420 WHIVDDQSFPYHSEKFPELSRLGAY 494
WHIVD+QSFP + +P L + GAY
Sbjct: 221 WHIVDEQSFPLETPTYPNLWK-GAY 244
>At4g16510.1 68417.m02498 YbaK/prolyl-tRNA synthetase-related
contains weak hit to Pfam profile PF04073:
YbaK/prolyl-tRNA synthetases associated domain
Length = 232
Score = 27.9 bits (59), Expect = 4.1
Identities = 17/44 (38%), Positives = 25/44 (56%)
Frame = +3
Query: 258 NDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSLSKILKNIDA 389
NDF R+ +TD YD+P + R +L +S +L S +L N A
Sbjct: 66 NDFCFKRV-ATDYYDWPLESRRDVLGASSVDHLCKSIVLVNTQA 108
>At1g79800.1 68414.m09316 plastocyanin-like domain-containing
protein
Length = 192
Score = 27.1 bits (57), Expect = 7.2
Identities = 13/42 (30%), Positives = 22/42 (52%)
Frame = +3
Query: 372 LKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLGAYH 497
L+N A+ ++ + +HI D SF Y + E+ + G YH
Sbjct: 46 LQNDSAVYSHWASSNRFHIGDSLSFVYDKDSVMEVDKWGFYH 87
>At1g47840.1 68414.m05325 hexokinase, putative similar to hexokinase
1 [Arabidopsis thaliana] Swiss-Prot:Q42525
Length = 493
Score = 27.1 bits (57), Expect = 7.2
Identities = 13/30 (43%), Positives = 18/30 (60%)
Frame = -1
Query: 423 ANGVRSFYSRPWHRYSLVSSITINSAWMCP 334
A G +F SRP Y ++S++ NSA CP
Sbjct: 13 ALGSFTFSSRPRSNYIVMSAVRSNSASTCP 42
>At5g36820.1 68418.m04411 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 410
Score = 26.6 bits (56), Expect = 9.5
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = -1
Query: 498 DDKLRVDLVRETFHCD 451
DD++RVD + + FHCD
Sbjct: 100 DDRVRVDDICKVFHCD 115
>At5g36730.1 68418.m04395 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 410
Score = 26.6 bits (56), Expect = 9.5
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = -1
Query: 498 DDKLRVDLVRETFHCD 451
DD++RVD + + FHCD
Sbjct: 100 DDRVRVDDICKVFHCD 115
>At1g18460.1 68414.m02303 lipase family protein similar to
triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
{Canis familiaris} [SP|P80035]
Length = 701
Score = 26.6 bits (56), Expect = 9.5
Identities = 13/34 (38%), Positives = 16/34 (47%)
Frame = -1
Query: 159 SFHPCQSKGASRTVQSVPRATPELSLGIVHRLQL 58
S PC SK S VP T + G++ LQL
Sbjct: 178 SSRPCFSKAISGKEHDVPNRTTDKRRGVIEDLQL 211
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,634,803
Number of Sequences: 28952
Number of extensions: 206011
Number of successful extensions: 498
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 491
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 495
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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