BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_D03 (504 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co... 106 7e-24 At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si... 99 8e-22 At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si... 97 4e-21 At4g16510.1 68417.m02498 YbaK/prolyl-tRNA synthetase-related con... 28 4.1 At1g79800.1 68414.m09316 plastocyanin-like domain-containing pro... 27 7.2 At1g47840.1 68414.m05325 hexokinase, putative similar to hexokin... 27 7.2 At5g36820.1 68418.m04411 F-box family protein contains Pfam PF00... 27 9.5 At5g36730.1 68418.m04395 F-box family protein contains Pfam PF00... 27 9.5 At1g18460.1 68414.m02303 lipase family protein similar to triacy... 27 9.5 >At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein contains Pfam PF00728: Glycosyl hydrolase family 20, catalytic domain; contains Pfam PF02838: Glycosyl hydrolase family 20, domain 2; similar to Beta-hexosaminidase beta chain precursor (EC 3.2.1.52) (N-acetyl-beta- glucosaminidase) (Beta-N-acetylhexosaminidase) (Hexosaminidase B) (Swiss-Prot:P07686) [Homo sapiens] Length = 535 Score = 106 bits (255), Expect = 7e-24 Identities = 61/142 (42%), Positives = 83/142 (58%), Gaps = 7/142 (4%) Frame = +3 Query: 90 VQELHVELTAPCEKLPYFGMDESYNLXXXX-----XXXXXXXXIWGVIRGLETWSQL--F 248 +Q LHV +++ ++L Y G DESY L ++G + GL+T+SQL F Sbjct: 102 LQGLHVIISSSTDELEY-GADESYKLVVPSPEKPSYAQLEAKSVYGALHGLQTFSQLCHF 160 Query: 249 YLTNDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLHWHI 428 L E+ + +I D PR+++RGLL+DTSRHYL L I ID+M K+NVLHWHI Sbjct: 161 NLKKKVIEILMTPWNIIDQPRFSYRGLLIDTSRHYLPLPVIKNVIDSMTYAKLNVLHWHI 220 Query: 429 VDDQSFPYHSEKFPELSRLGAY 494 VD QSFP +P+L GAY Sbjct: 221 VDTQSFPLEIPSYPKLWN-GAY 241 >At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein similar to beta-hexosaminidase precursor SP:P43077 from [Candida albicans] Length = 580 Score = 99 bits (238), Expect = 8e-22 Identities = 52/124 (41%), Positives = 70/124 (56%), Gaps = 3/124 (2%) Frame = +3 Query: 138 YFGMDESYNLXX---XXXXXXXXXXIWGVIRGLETWSQLFYLTNDFRELRINSTDIYDYP 308 + G+DESY L WG +RGLET+SQ+ + T+ L + I D P Sbjct: 111 HHGVDESYKLSIPIGSFSAHLLAHSAWGAMRGLETFSQMIWGTSPDLCLPVGIY-IQDSP 169 Query: 309 RYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLG 488 + HRG+LLDTSR+Y + I++ I AM+ANK+NV HWHI D QSFP P L+ G Sbjct: 170 LFGHRGVLLDTSRNYYGVDDIMRTIKAMSANKLNVFHWHITDSQSFPLVLPSEPSLAAKG 229 Query: 489 AYHP 500 + P Sbjct: 230 SLGP 233 >At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein similar to beta-hexosaminidase A SP:P13723 from [Dictyostelium discoideum] Length = 541 Score = 97.5 bits (232), Expect = 4e-21 Identities = 56/145 (38%), Positives = 82/145 (56%), Gaps = 10/145 (6%) Frame = +3 Query: 90 VQELHVELTAPCEKLPYFGMDESYNLXXXX--------XXXXXXXXIWGVIRGLETWSQL 245 + L + + + E+L G+DESY L ++G +RGLET+SQL Sbjct: 102 ITSLKIVVHSDSEELQ-LGVDESYTLMVSKKNEQSIVGAATIEANTVYGALRGLETFSQL 160 Query: 246 FYLTNDFRELRINSTDIY--DYPRYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLH 419 + ++I Y D PR+ +RGLL+DTSRHYL + I + I++M+ K+NVLH Sbjct: 161 CAFDYITKSVQIYKAPWYIQDKPRFGYRGLLIDTSRHYLPIDVIKQIIESMSFAKLNVLH 220 Query: 420 WHIVDDQSFPYHSEKFPELSRLGAY 494 WHIVD+QSFP + +P L + GAY Sbjct: 221 WHIVDEQSFPLETPTYPNLWK-GAY 244 >At4g16510.1 68417.m02498 YbaK/prolyl-tRNA synthetase-related contains weak hit to Pfam profile PF04073: YbaK/prolyl-tRNA synthetases associated domain Length = 232 Score = 27.9 bits (59), Expect = 4.1 Identities = 17/44 (38%), Positives = 25/44 (56%) Frame = +3 Query: 258 NDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSLSKILKNIDA 389 NDF R+ +TD YD+P + R +L +S +L S +L N A Sbjct: 66 NDFCFKRV-ATDYYDWPLESRRDVLGASSVDHLCKSIVLVNTQA 108 >At1g79800.1 68414.m09316 plastocyanin-like domain-containing protein Length = 192 Score = 27.1 bits (57), Expect = 7.2 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = +3 Query: 372 LKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLGAYH 497 L+N A+ ++ + +HI D SF Y + E+ + G YH Sbjct: 46 LQNDSAVYSHWASSNRFHIGDSLSFVYDKDSVMEVDKWGFYH 87 >At1g47840.1 68414.m05325 hexokinase, putative similar to hexokinase 1 [Arabidopsis thaliana] Swiss-Prot:Q42525 Length = 493 Score = 27.1 bits (57), Expect = 7.2 Identities = 13/30 (43%), Positives = 18/30 (60%) Frame = -1 Query: 423 ANGVRSFYSRPWHRYSLVSSITINSAWMCP 334 A G +F SRP Y ++S++ NSA CP Sbjct: 13 ALGSFTFSSRPRSNYIVMSAVRSNSASTCP 42 >At5g36820.1 68418.m04411 F-box family protein contains Pfam PF00646: F-box domain; contains TIGRFAM TIGR01640 : F-box protein interaction domain Length = 410 Score = 26.6 bits (56), Expect = 9.5 Identities = 9/16 (56%), Positives = 13/16 (81%) Frame = -1 Query: 498 DDKLRVDLVRETFHCD 451 DD++RVD + + FHCD Sbjct: 100 DDRVRVDDICKVFHCD 115 >At5g36730.1 68418.m04395 F-box family protein contains Pfam PF00646: F-box domain; contains TIGRFAM TIGR01640 : F-box protein interaction domain Length = 410 Score = 26.6 bits (56), Expect = 9.5 Identities = 9/16 (56%), Positives = 13/16 (81%) Frame = -1 Query: 498 DDKLRVDLVRETFHCD 451 DD++RVD + + FHCD Sbjct: 100 DDRVRVDDICKVFHCD 115 >At1g18460.1 68414.m02303 lipase family protein similar to triacylglycerol lipase, gastric precursor (EC 3.1.1.3) {Canis familiaris} [SP|P80035] Length = 701 Score = 26.6 bits (56), Expect = 9.5 Identities = 13/34 (38%), Positives = 16/34 (47%) Frame = -1 Query: 159 SFHPCQSKGASRTVQSVPRATPELSLGIVHRLQL 58 S PC SK S VP T + G++ LQL Sbjct: 178 SSRPCFSKAISGKEHDVPNRTTDKRRGVIEDLQL 211 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,634,803 Number of Sequences: 28952 Number of extensions: 206011 Number of successful extensions: 498 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 491 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 495 length of database: 12,070,560 effective HSP length: 76 effective length of database: 9,870,208 effective search space used: 898188928 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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