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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_D03
         (504 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein co...   106   7e-24
At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein si...    99   8e-22
At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein si...    97   4e-21
At4g16510.1 68417.m02498 YbaK/prolyl-tRNA synthetase-related con...    28   4.1  
At1g79800.1 68414.m09316 plastocyanin-like domain-containing pro...    27   7.2  
At1g47840.1 68414.m05325 hexokinase, putative similar to hexokin...    27   7.2  
At5g36820.1 68418.m04411 F-box family protein contains Pfam PF00...    27   9.5  
At5g36730.1 68418.m04395 F-box family protein contains Pfam PF00...    27   9.5  
At1g18460.1 68414.m02303 lipase family protein similar to triacy...    27   9.5  

>At1g65590.1 68414.m07441 glycosyl hydrolase family 20 protein
           contains Pfam PF00728: Glycosyl hydrolase family 20,
           catalytic domain; contains Pfam PF02838: Glycosyl
           hydrolase family 20, domain 2; similar to
           Beta-hexosaminidase beta chain precursor (EC 3.2.1.52)
           (N-acetyl-beta- glucosaminidase)
           (Beta-N-acetylhexosaminidase) (Hexosaminidase B)
           (Swiss-Prot:P07686) [Homo sapiens]
          Length = 535

 Score =  106 bits (255), Expect = 7e-24
 Identities = 61/142 (42%), Positives = 83/142 (58%), Gaps = 7/142 (4%)
 Frame = +3

Query: 90  VQELHVELTAPCEKLPYFGMDESYNLXXXX-----XXXXXXXXIWGVIRGLETWSQL--F 248
           +Q LHV +++  ++L Y G DESY L                 ++G + GL+T+SQL  F
Sbjct: 102 LQGLHVIISSSTDELEY-GADESYKLVVPSPEKPSYAQLEAKSVYGALHGLQTFSQLCHF 160

Query: 249 YLTNDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLHWHI 428
            L     E+ +   +I D PR+++RGLL+DTSRHYL L  I   ID+M   K+NVLHWHI
Sbjct: 161 NLKKKVIEILMTPWNIIDQPRFSYRGLLIDTSRHYLPLPVIKNVIDSMTYAKLNVLHWHI 220

Query: 429 VDDQSFPYHSEKFPELSRLGAY 494
           VD QSFP     +P+L   GAY
Sbjct: 221 VDTQSFPLEIPSYPKLWN-GAY 241


>At1g05590.1 68414.m00579 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase precursor SP:P43077 from
           [Candida albicans]
          Length = 580

 Score =   99 bits (238), Expect = 8e-22
 Identities = 52/124 (41%), Positives = 70/124 (56%), Gaps = 3/124 (2%)
 Frame = +3

Query: 138 YFGMDESYNLXX---XXXXXXXXXXIWGVIRGLETWSQLFYLTNDFRELRINSTDIYDYP 308
           + G+DESY L                WG +RGLET+SQ+ + T+    L +    I D P
Sbjct: 111 HHGVDESYKLSIPIGSFSAHLLAHSAWGAMRGLETFSQMIWGTSPDLCLPVGIY-IQDSP 169

Query: 309 RYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLG 488
            + HRG+LLDTSR+Y  +  I++ I AM+ANK+NV HWHI D QSFP      P L+  G
Sbjct: 170 LFGHRGVLLDTSRNYYGVDDIMRTIKAMSANKLNVFHWHITDSQSFPLVLPSEPSLAAKG 229

Query: 489 AYHP 500
           +  P
Sbjct: 230 SLGP 233


>At3g55260.1 68416.m06137 glycosyl hydrolase family 20 protein
           similar to beta-hexosaminidase A SP:P13723 from
           [Dictyostelium discoideum]
          Length = 541

 Score = 97.5 bits (232), Expect = 4e-21
 Identities = 56/145 (38%), Positives = 82/145 (56%), Gaps = 10/145 (6%)
 Frame = +3

Query: 90  VQELHVELTAPCEKLPYFGMDESYNLXXXX--------XXXXXXXXIWGVIRGLETWSQL 245
           +  L + + +  E+L   G+DESY L                    ++G +RGLET+SQL
Sbjct: 102 ITSLKIVVHSDSEELQ-LGVDESYTLMVSKKNEQSIVGAATIEANTVYGALRGLETFSQL 160

Query: 246 FYLTNDFRELRINSTDIY--DYPRYAHRGLLLDTSRHYLSLSKILKNIDAMAANKMNVLH 419
                  + ++I     Y  D PR+ +RGLL+DTSRHYL +  I + I++M+  K+NVLH
Sbjct: 161 CAFDYITKSVQIYKAPWYIQDKPRFGYRGLLIDTSRHYLPIDVIKQIIESMSFAKLNVLH 220

Query: 420 WHIVDDQSFPYHSEKFPELSRLGAY 494
           WHIVD+QSFP  +  +P L + GAY
Sbjct: 221 WHIVDEQSFPLETPTYPNLWK-GAY 244


>At4g16510.1 68417.m02498 YbaK/prolyl-tRNA synthetase-related
           contains weak hit to Pfam profile PF04073:
           YbaK/prolyl-tRNA synthetases associated domain
          Length = 232

 Score = 27.9 bits (59), Expect = 4.1
 Identities = 17/44 (38%), Positives = 25/44 (56%)
 Frame = +3

Query: 258 NDFRELRINSTDIYDYPRYAHRGLLLDTSRHYLSLSKILKNIDA 389
           NDF   R+ +TD YD+P  + R +L  +S  +L  S +L N  A
Sbjct: 66  NDFCFKRV-ATDYYDWPLESRRDVLGASSVDHLCKSIVLVNTQA 108


>At1g79800.1 68414.m09316 plastocyanin-like domain-containing
           protein
          Length = 192

 Score = 27.1 bits (57), Expect = 7.2
 Identities = 13/42 (30%), Positives = 22/42 (52%)
 Frame = +3

Query: 372 LKNIDAMAANKMNVLHWHIVDDQSFPYHSEKFPELSRLGAYH 497
           L+N  A+ ++  +   +HI D  SF Y  +   E+ + G YH
Sbjct: 46  LQNDSAVYSHWASSNRFHIGDSLSFVYDKDSVMEVDKWGFYH 87


>At1g47840.1 68414.m05325 hexokinase, putative similar to hexokinase
           1 [Arabidopsis thaliana] Swiss-Prot:Q42525
          Length = 493

 Score = 27.1 bits (57), Expect = 7.2
 Identities = 13/30 (43%), Positives = 18/30 (60%)
 Frame = -1

Query: 423 ANGVRSFYSRPWHRYSLVSSITINSAWMCP 334
           A G  +F SRP   Y ++S++  NSA  CP
Sbjct: 13  ALGSFTFSSRPRSNYIVMSAVRSNSASTCP 42


>At5g36820.1 68418.m04411 F-box family protein contains Pfam
           PF00646: F-box domain; contains TIGRFAM TIGR01640 :
           F-box protein interaction domain
          Length = 410

 Score = 26.6 bits (56), Expect = 9.5
 Identities = 9/16 (56%), Positives = 13/16 (81%)
 Frame = -1

Query: 498 DDKLRVDLVRETFHCD 451
           DD++RVD + + FHCD
Sbjct: 100 DDRVRVDDICKVFHCD 115


>At5g36730.1 68418.m04395 F-box family protein contains Pfam
           PF00646: F-box domain; contains TIGRFAM TIGR01640 :
           F-box protein interaction domain
          Length = 410

 Score = 26.6 bits (56), Expect = 9.5
 Identities = 9/16 (56%), Positives = 13/16 (81%)
 Frame = -1

Query: 498 DDKLRVDLVRETFHCD 451
           DD++RVD + + FHCD
Sbjct: 100 DDRVRVDDICKVFHCD 115


>At1g18460.1 68414.m02303 lipase family protein similar to
           triacylglycerol lipase, gastric precursor (EC 3.1.1.3)
           {Canis familiaris} [SP|P80035]
          Length = 701

 Score = 26.6 bits (56), Expect = 9.5
 Identities = 13/34 (38%), Positives = 16/34 (47%)
 Frame = -1

Query: 159 SFHPCQSKGASRTVQSVPRATPELSLGIVHRLQL 58
           S  PC SK  S     VP  T +   G++  LQL
Sbjct: 178 SSRPCFSKAISGKEHDVPNRTTDKRRGVIEDLQL 211


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,634,803
Number of Sequences: 28952
Number of extensions: 206011
Number of successful extensions: 498
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 491
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 495
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 898188928
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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