Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_C15
(542 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 60 1e-09
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 58 5e-09
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 39 0.002
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 38 0.006
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 36 0.013
At3g51070.1 68416.m05592 dehydration-responsive protein-related ... 31 0.66
At1g27385.1 68414.m03338 expressed protein 29 2.7
At5g05190.1 68418.m00553 expressed protein similar to unknown pr... 28 3.5
At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEME... 28 3.5
At5g33230.1 68418.m03927 hypothetical protein 27 6.1
At4g37750.1 68417.m05344 ovule development protein aintegumenta ... 27 6.1
At1g30660.1 68414.m03749 toprim domain-containing protein contai... 27 6.1
At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family prot... 27 8.1
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 59.7 bits (138), Expect = 1e-09
Identities = 26/73 (35%), Positives = 43/73 (58%)
Frame = +1
Query: 292 REQLKPQESYILDTVSGNIYVWIGKQSTEKEKSEAMTKAQQTLSAKNYPSWVHVTRVLQG 471
+ L+ + Y+LD IY+W+G+ + E+ A A++ L+++N P HVTRV+QG
Sbjct: 271 KSMLENTKCYLLDC-GAEIYIWVGRVTQVDERKAASQSAEEFLASENRPKATHVTRVIQG 329
Query: 472 TEPAAFKQYFSTW 510
E +FK F +W
Sbjct: 330 YESHSFKSNFDSW 342
Score = 36.7 bits (81), Expect = 0.010
Identities = 20/75 (26%), Positives = 35/75 (46%), Gaps = 4/75 (5%)
Frame = +1
Query: 292 REQLKPQESYILDTVSGNIYVWIGKQSTEKEKSEAMTKAQQTLSAKNYPSWVH----VTR 459
++ L ++ Y LDT ++VW+G+ KEK Q+ + +H + +
Sbjct: 643 QDDLLTEDIYFLDT-HAEVFVWVGQCVEPKEKQTVFEIGQKYIDLAGSLEGLHPKVPIYK 701
Query: 460 VLQGTEPAAFKQYFS 504
+ +G EP F YFS
Sbjct: 702 INEGNEPCFFTTYFS 716
Score = 27.5 bits (58), Expect = 6.1
Identities = 16/53 (30%), Positives = 25/53 (47%)
Frame = +1
Query: 343 NIYVWIGKQSTEKEKSEAMTKAQQTLSAKNYPSWVHVTRVLQGTEPAAFKQYF 501
+I+ WIGK +++ E A K + + + H R +QG E F YF
Sbjct: 62 DIHFWIGKDTSQDEAGTAAVKTVELDAVLGGRAVQH--REIQGHESDKFLSYF 112
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 57.6 bits (133), Expect = 5e-09
Identities = 24/73 (32%), Positives = 45/73 (61%)
Frame = +1
Query: 292 REQLKPQESYILDTVSGNIYVWIGKQSTEKEKSEAMTKAQQTLSAKNYPSWVHVTRVLQG 471
+ L+ + Y+LD S I++W+G+ + +E+ A+ A+ ++++N P +TRV+QG
Sbjct: 273 KSMLENNKCYLLDCGS-EIFIWVGRVTQVEERKTAIQAAEDFVASENRPKATRITRVIQG 331
Query: 472 TEPAAFKQYFSTW 510
EP +FK F +W
Sbjct: 332 YEPHSFKSNFDSW 344
Score = 38.7 bits (86), Expect = 0.002
Identities = 22/75 (29%), Positives = 38/75 (50%), Gaps = 4/75 (5%)
Frame = +1
Query: 292 REQLKPQESYILDTVSGNIYVWIGKQSTEKEKSEAMTKAQQTL----SAKNYPSWVHVTR 459
++ L +E ++LDT ++VW+G+ KEK A Q+ + S + V + +
Sbjct: 645 QDDLLTEEMHLLDT-HAEVFVWVGQCVDPKEKQTAFEIGQRYINLAGSLEGLSPKVPLYK 703
Query: 460 VLQGTEPAAFKQYFS 504
+ +G EP F YFS
Sbjct: 704 ITEGNEPCFFTTYFS 718
Score = 27.1 bits (57), Expect = 8.1
Identities = 13/45 (28%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +3
Query: 15 EKLKAISMANQIRDQDHNGRGRVEIID--QYSNEVDTQKFFTALG 143
E+ KA+ + ++D+ H G V I+D + E D+ +F+ G
Sbjct: 188 ERAKALVVIQYLKDKFHEGTSDVAIVDDGKLDTESDSGEFWVLFG 232
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 38.7 bits (86), Expect = 0.002
Identities = 25/88 (28%), Positives = 47/88 (53%), Gaps = 4/88 (4%)
Frame = +1
Query: 259 VKATGLARPWRREQLKPQESYILDTVSGNIYVWIGKQSTEKEKSEAMTKAQQTLS----A 426
+KAT + + ++ L ++ +ILD + ++VW+G+Q K+K +A+ + L
Sbjct: 636 LKATEIFN-FTQDDLMTEDIFILDCHT-EVFVWVGQQVDPKKKPQALDIGENFLKHDFLL 693
Query: 427 KNYPSWVHVTRVLQGTEPAAFKQYFSTW 510
+N S + V +G EP F ++F TW
Sbjct: 694 ENLASETPIYIVTEGNEPPFFTRFF-TW 720
Score = 29.5 bits (63), Expect = 1.5
Identities = 16/46 (34%), Positives = 27/46 (58%), Gaps = 1/46 (2%)
Frame = +1
Query: 355 WIGKQSTEKEKSEAMTKAQQTL-SAKNYPSWVHVTRVLQGTEPAAF 489
W GKQS E++++ A++ A + + S K P+ R+ +G EP F
Sbjct: 442 WFGKQSVEEDRASAISLANKMVESMKFVPA---QARINEGKEPIQF 484
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 37.5 bits (83), Expect = 0.006
Identities = 21/78 (26%), Positives = 44/78 (56%), Gaps = 5/78 (6%)
Frame = +1
Query: 292 REQLKPQESYILDTVSGNIYVWIGKQSTEKEKSEAMTKAQQ-----TLSAKNYPSWVHVT 456
++ L ++ +I+D S I+VW+G++ K K A+T ++ +L K P +
Sbjct: 644 QDDLMTEDIFIIDCHS-EIFVWVGQEVVPKNKLLALTIGEKFIEKDSLLEKLSPE-APIY 701
Query: 457 RVLQGTEPAAFKQYFSTW 510
+++G EP+ F ++F++W
Sbjct: 702 VIMEGGEPSFFTRFFTSW 719
Score = 31.9 bits (69), Expect = 0.28
Identities = 18/49 (36%), Positives = 27/49 (55%), Gaps = 1/49 (2%)
Frame = +1
Query: 346 IYVWIGKQSTEKEKSEAMTKAQQTL-SAKNYPSWVHVTRVLQGTEPAAF 489
I W GKQS E+E+ A++ A + + S K P+ R+ +G EP F
Sbjct: 437 IGTWFGKQSVEEERGSAVSMASKMVESMKFVPA---QARIYEGKEPIQF 482
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 36.3 bits (80), Expect = 0.013
Identities = 22/74 (29%), Positives = 36/74 (48%), Gaps = 4/74 (5%)
Frame = +1
Query: 292 REQLKPQESYILDTVSGNIYVWIGKQSTEKEKSEAMTKA----QQTLSAKNYPSWVHVTR 459
++ L ++ ++LD S +YVWIG S K K EA+T + + + V
Sbjct: 641 QDDLTTEDVFLLDCQS-EVYVWIGSNSNIKSKEEALTLGLKFLEMDILEEGLTMRTPVYV 699
Query: 460 VLQGTEPAAFKQYF 501
V +G EP F ++F
Sbjct: 700 VTEGHEPPFFTRFF 713
Score = 34.3 bits (75), Expect = 0.053
Identities = 16/54 (29%), Positives = 30/54 (55%)
Frame = +1
Query: 346 IYVWIGKQSTEKEKSEAMTKAQQTLSAKNYPSWVHVTRVLQGTEPAAFKQYFST 507
+YVWIG +S ++++++A+T A + S + + QG EP+ F F +
Sbjct: 434 LYVWIGCESIQQDRADAITNASAIVGTTKGES--VLCHIYQGNEPSRFFPMFQS 485
Score = 31.1 bits (67), Expect = 0.50
Identities = 15/45 (33%), Positives = 26/45 (57%), Gaps = 2/45 (4%)
Frame = +3
Query: 15 EKLKAISMANQIRDQDHNGRGRVEIID--QYSNEVDTQKFFTALG 143
EK KA+ + I+D H+GR V I+ ++S + D +F++ G
Sbjct: 187 EKAKAMEVVEYIKDNKHDGRCEVATIEDGKFSGDSDAGEFWSFFG 231
>At3g51070.1 68416.m05592 dehydration-responsive protein-related
similar to early-responsive to dehydration stress ERD3
protein [Arabidopsis thaliana] GI:15320410; contains
Pfam profile PF03141: Putative methyltransferase
Length = 895
Score = 30.7 bits (66), Expect = 0.66
Identities = 17/47 (36%), Positives = 25/47 (53%)
Frame = +3
Query: 165 PEESAGGDDQAFERNEEQTVSLSEVSDSSGKSQGDGPGQAMASGTVE 305
PEE G+++ ++NEE+T + SE + KS D GQ T E
Sbjct: 243 PEEQNSGNEETGQQNEEKTTA-SEENGKGEKSMKDENGQQEEHTTAE 288
>At1g27385.1 68414.m03338 expressed protein
Length = 202
Score = 28.7 bits (61), Expect = 2.7
Identities = 20/72 (27%), Positives = 31/72 (43%)
Frame = +3
Query: 90 IDQYSNEVDTQKFFTALGSGTRDIVPEESAGGDDQAFERNEEQTVSLSEVSDSSGKSQGD 269
+DQ N T++ FTA+G+G D V + + E V + S S
Sbjct: 115 LDQKVNTYPTERGFTAIGTGGNDFVHAMVVAVESVIDRQIPEDCVKQTLSSKGKYVSVNI 174
Query: 270 GPGQAMASGTVE 305
GP Q ++S V+
Sbjct: 175 GPIQVVSSEQVQ 186
>At5g05190.1 68418.m00553 expressed protein similar to unknown
protein (emb|CAB88044.1)
Length = 615
Score = 28.3 bits (60), Expect = 3.5
Identities = 18/76 (23%), Positives = 37/76 (48%), Gaps = 3/76 (3%)
Frame = +3
Query: 54 DQDHNGRGRVEIIDQYSNEVDTQKFFTALGSGTRDIVPEESAGGDDQAFERNEEQTVS-- 227
DQ++ +GR ++ E+D + G GT +I +E + GD + ER + +
Sbjct: 93 DQEYE-KGRNASMESTEKELDDLELSN--GDGTNEIQEQECSLGDSEKNEREDNSRLESH 149
Query: 228 -LSEVSDSSGKSQGDG 272
++ V++++G G
Sbjct: 150 MMNTVAEAAGSGSSSG 165
>At5g04560.1 68418.m00456 DEMETER protein (DME) identical to DEMETER
protein [Arabidopsis thaliana] GI:21743571; contains
Pfam profile PF00730: HhH-GPD superfamily base excision
DNA repair protein
Length = 1729
Score = 28.3 bits (60), Expect = 3.5
Identities = 25/105 (23%), Positives = 46/105 (43%), Gaps = 7/105 (6%)
Frame = +1
Query: 121 KSSSQPSDQAPG----I*CRKKVPEVTIKLSK---GTKNRL*VFQKYQTAAVKVKATGLA 279
+ S Q S +A G + C+ + E+ ++ G K R ++ Q A V K G
Sbjct: 629 RKSIQDSGKARGPSGELLCQDSIAEIIYRMQNLYLGDKER----EQEQNAMVLYKGDGAL 684
Query: 280 RPWRREQLKPQESYILDTVSGNIYVWIGKQSTEKEKSEAMTKAQQ 414
P+ ++ KP+ +D + I+ + + EKE E K ++
Sbjct: 685 VPYESKKRKPRPKVDIDDETTRIWNLLMGKGDEKEGDEEKDKKKE 729
>At5g33230.1 68418.m03927 hypothetical protein
Length = 126
Score = 27.5 bits (58), Expect = 6.1
Identities = 16/54 (29%), Positives = 27/54 (50%), Gaps = 3/54 (5%)
Frame = +3
Query: 114 DTQKFFTALGSGTRDIVP---EESAGGDDQAFERNEEQTVSLSEVSDSSGKSQG 266
DTQ + SG+RD VP E + G D++ E ++++ ++S K G
Sbjct: 39 DTQSKALVIHSGSRDPVPPTIESLSDGSDESDEEDKKKKKKKKQISKKKRKHVG 92
>At4g37750.1 68417.m05344 ovule development protein aintegumenta
(ANT) identical to ovule development protein
aintegumenta (ANT) (GI:1244708) ) [Arabidopsis thaliana]
Length = 555
Score = 27.5 bits (58), Expect = 6.1
Identities = 13/38 (34%), Positives = 19/38 (50%)
Frame = -3
Query: 507 GREVLFEGSRLSALQYSGYVDPRWIIFS*QSLLGFCHG 394
GRE ++ S SA S V P+ ++ S G C+G
Sbjct: 35 GREAIYSSSTSSAATSSSSVPPQLVVGDNTSNFGVCYG 72
>At1g30660.1 68414.m03749 toprim domain-containing protein contains
Pfam profile PF01751: Toprim domain
Length = 321
Score = 27.5 bits (58), Expect = 6.1
Identities = 19/59 (32%), Positives = 23/59 (38%), Gaps = 6/59 (10%)
Frame = +3
Query: 216 QTVSLSEVSDSSGKSQ------GDGPGQAMASGTVETTGVVHPGYCKWEHLRMDREAVD 374
+TVS E+ S S+ GDGPGQA+A G KW D D
Sbjct: 236 ETVSSKEIPSESKASRIVIATDGDGPGQALAEELARRLGKERCWLVKWPKKSEDEHFKD 294
>At5g03980.1 68418.m00378 GDSL-motif lipase/hydrolase family protein
similar to lipase [Arabidopsis thaliana] GI:1145627;
contains Pfam profile:PF00657 Lipase/Acylhydrolase with
GDSL-like motif
Length = 323
Score = 27.1 bits (57), Expect = 8.1
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = -1
Query: 383 SFSVDCFPIHT*MFPLTVSRMYDSCG 306
+F V CFPI+ FP+ ++ YD G
Sbjct: 177 NFPVGCFPIYLTSFPVKDTKDYDDNG 202
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,117,693
Number of Sequences: 28952
Number of extensions: 247883
Number of successful extensions: 861
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 823
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 860
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1013649368
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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