Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_C11
(536 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g12870.1 68417.m02015 gamma interferon responsive lysosomal t... 66 1e-11
At4g12960.1 68417.m02025 gamma interferon responsive lysosomal t... 66 2e-11
At4g12900.1 68417.m02018 gamma interferon responsive lysosomal t... 66 2e-11
At4g12890.1 68417.m02017 gamma interferon responsive lysosomal t... 64 7e-11
At1g07080.1 68414.m00754 gamma interferon responsive lysosomal t... 52 2e-07
At5g01580.1 68418.m00073 gamma interferon responsive lysosomal t... 52 3e-07
At4g38350.1 68417.m05422 patched family protein similar to SP|O1... 29 2.0
At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing pr... 27 6.0
>At4g12870.1 68417.m02015 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 229
Score = 66.5 bits (155), Expect = 1e-11
Identities = 37/106 (34%), Positives = 56/106 (52%), Gaps = 1/106 (0%)
Frame = +3
Query: 69 SYKYKMKLLLLICAVTMICGVLAKNKKTDDQKVKIAVYYESLCPDSKKFITTQLAPVW-R 245
S + KL+ C V ++D KV++ +YYESLCP + FI +L V+
Sbjct: 3 SPSFSTKLVFFACFVLFTFSHKLVTGESD--KVELNLYYESLCPGCQSFIVDELVKVFDS 60
Query: 246 DFRGLVKVKLVPYGKSTHDKVNEKWTFTCHHGPDECYGNKMQACIL 383
D + VKLVP+G + KV+ T C HG +EC N ++AC++
Sbjct: 61 DLDTITDVKLVPFG---YAKVSNNLTVICQHGEEECKLNALEACVI 103
>At4g12960.1 68417.m02025 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 65.7 bits (153), Expect = 2e-11
Identities = 37/115 (32%), Positives = 64/115 (55%), Gaps = 1/115 (0%)
Frame = +3
Query: 162 KVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKVKLVPYGKSTHDKVNEKWTFTCHH 338
KVK+ +YYESLCP ++FI L ++ D + +KL P+G + ++++ T TC H
Sbjct: 31 KVKLNLYYESLCPGCQEFIVDDLGKIFDYDLYTITDLKLFPFGNA---ELSDNLTVTCQH 87
Query: 339 GPDECYGNKMQACILKDRSLQDTEKMELVICLMSQASPDKSLDTCLISSEQTKRI 503
G +EC N ++AC L+ Q ++ + C+ S K ++C+ +S + K I
Sbjct: 88 GEEECKLNALEACALRTWPDQKSQ-YSFIRCVESDT---KGWESCVKNSGREKAI 138
>At4g12900.1 68417.m02018 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 231
Score = 65.7 bits (153), Expect = 2e-11
Identities = 29/79 (36%), Positives = 47/79 (59%), Gaps = 1/79 (1%)
Frame = +3
Query: 153 DDQKVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVKVKLVPYGKSTHDKVNEKWTFT 329
+ KVK+ +YYESLCP + FI L ++ D + +KL+P+G + V++ T T
Sbjct: 33 ESDKVKLNLYYESLCPSCQNFIVHHLGKIFNTDLHTITDLKLIPFGNA---HVSDDLTVT 89
Query: 330 CHHGPDECYGNKMQACILK 386
C HG +EC N ++AC ++
Sbjct: 90 CQHGEEECKLNALEACAIR 108
>At4g12890.1 68417.m02017 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 232
Score = 63.7 bits (148), Expect = 7e-11
Identities = 38/119 (31%), Positives = 64/119 (53%), Gaps = 2/119 (1%)
Frame = +3
Query: 153 DDQKVKIAVYYESLCPDSKKFITTQLAPVW-RDFRGLVKVKLVPYGKSTHDKVNEKWTFT 329
+ KVKI +YYESLCP + FI L ++ D + +KLVP+G + ++ T T
Sbjct: 35 NSNKVKINLYYESLCPYCQNFIVDDLGKIFDSDLLKITDLKLVPFGNA---HISNNLTIT 91
Query: 330 CHHGPDECYGNKMQACILKDRSLQDTE-KMELVICLMSQASPDKSLDTCLISSEQTKRI 503
C HG +EC N ++AC + R+L D + + + + C+ + ++C+ S + K I
Sbjct: 92 CQHGEEECKLNALEACGI--RTLPDPKLQYKFIRCVEKDTN---EWESCVKKSGREKAI 145
>At1g07080.1 68414.m00754 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 265
Score = 52.4 bits (120), Expect = 2e-07
Identities = 27/75 (36%), Positives = 40/75 (53%), Gaps = 1/75 (1%)
Frame = +3
Query: 162 KVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVKVKLVPYGKSTHDKVNEKWTFTCHH 338
KV + +YYESLCP FI LA ++ D +V + L P+G + N T C H
Sbjct: 38 KVSVGLYYESLCPYCSSFIVNHLAKLFEDDLISIVDLHLSPWGNTKLRSDNV--TAVCQH 95
Query: 339 GPDECYGNKMQACIL 383
G EC+ + ++AC +
Sbjct: 96 GAFECFLDTVEACAI 110
>At5g01580.1 68418.m00073 gamma interferon responsive lysosomal
thiol reductase family protein / GILT family protein
similar to SP|P13284 Gamma-interferon inducible
lysosomal thiol reductase precursor {Homo sapiens};
contains Pfam profile PF03227: Gamma interferon
inducible lysosomal thiol reductase (GILT)
Length = 233
Score = 51.6 bits (118), Expect = 3e-07
Identities = 25/79 (31%), Positives = 42/79 (53%), Gaps = 1/79 (1%)
Frame = +3
Query: 150 TDDQKVKIAVYYESLCPDSKKFITTQLAPVWR-DFRGLVKVKLVPYGKSTHDKVNEKWTF 326
+ QKV +++YYE+LCP +FI +L ++ + ++LVP+G + + T
Sbjct: 22 SSSQKVTLSLYYEALCPFCAEFIVNRLPKIFETGLISSIDLQLVPWGNAA---IRPDGTI 78
Query: 327 TCHHGPDECYGNKMQACIL 383
C HG EC N + AC +
Sbjct: 79 LCQHGEAECALNAIHACAI 97
>At4g38350.1 68417.m05422 patched family protein similar to
SP|O15118 Niemann-Pick C1 protein precursor from Homo
sapiens (PID:g2276463); contains Pfam profile PF02460
Patched family
Length = 1064
Score = 29.1 bits (62), Expect = 2.0
Identities = 17/52 (32%), Positives = 26/52 (50%)
Frame = +3
Query: 90 LLLLICAVTMICGVLAKNKKTDDQKVKIAVYYESLCPDSKKFITTQLAPVWR 245
L + + V +C L N K + + K+ V ES + KKF T L+P +R
Sbjct: 311 LFMSVAIVLALCSGLY-NFKVETRPEKLWVGPESKAAEEKKFFDTHLSPFYR 361
>At4g00416.1 68417.m00056 methyl-CpG-binding domain-containing
protein contains Pfam profile PF01429: Methyl-CpG
binding domain
Length = 163
Score = 27.5 bits (58), Expect = 6.0
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +3
Query: 165 VKIAVYYESLCPDSKKF 215
VK+ VYYESL P K+F
Sbjct: 90 VKVDVYYESLAPRRKRF 106
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,525,760
Number of Sequences: 28952
Number of extensions: 222997
Number of successful extensions: 597
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 584
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 591
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 993966856
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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