Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_C09
(661 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g40770.1 68415.m05030 SNF2 domain-containing protein / helica... 30 1.2
At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH) domain... 29 2.1
At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH) domain... 29 2.7
At3g61160.2 68416.m06845 shaggy-related protein kinase beta / AS... 28 4.8
At3g61160.1 68416.m06844 shaggy-related protein kinase beta / AS... 28 4.8
At1g64290.1 68414.m07285 F-box protein-related contains TIGRFAM ... 28 4.8
At1g13260.1 68414.m01539 DNA-binding protein RAV1 (RAV1) identic... 28 6.3
At5g63580.1 68418.m07981 flavonol synthase, putative similar to ... 27 8.4
At3g54930.1 68416.m06087 serine/threonine protein phosphatase 2A... 27 8.4
At2g45870.1 68415.m05705 expressed protein contains Pfam profile... 27 8.4
>At2g40770.1 68415.m05030 SNF2 domain-containing protein / helicase
domain-containing protein / zinc finger (C3HC4 type RING
finger) family protein low similarity to SP|P36607 DNA
repair protein rad8 {Schizosaccharomyces pombe};
contains Pfam profiles PF00271: Helicase conserved
C-terminal domain, PF00176: SNF2 family N-terminal
domain, PF00628: PHD-finger, PF00097: Zinc finger, C3HC4
type (RING finger)
Length = 1648
Score = 30.3 bits (65), Expect = 1.2
Identities = 14/48 (29%), Positives = 24/48 (50%)
Frame = +2
Query: 14 ARKLISTMKRQLTLSETIGQRTPICMKKKLQRIINDLMKLSLAMCSVQ 157
AR++I T+KR + P+ + +++N L+KL A C Q
Sbjct: 719 AREVIETLKRDILKRGHTSSDNPLVTHAEAAKLLNSLLKLRQACCHPQ 766
>At4g25940.1 68417.m03731 epsin N-terminal homology (ENTH)
domain-containing protein contains Pfam PF01417: ENTH
domain. ENTH (Epsin N-terminal homology) domain; similar
to Chain B, Crystal Structure Of N-Terminal Domain Of
Drosophila Ap180 (GP:13399617) [Drosophila
melanogaster]; supporting cDNA
gi|20465326|gb|AY096427.1|
Length = 601
Score = 29.5 bits (63), Expect = 2.1
Identities = 35/132 (26%), Positives = 59/132 (44%), Gaps = 14/132 (10%)
Frame = +1
Query: 67 WPENADLYEEEVTKDYQ-RSYEIVARHV---LGAAPKPFD---KHTFMPSALDFYQTALR 225
W L+ EE + Y+ Y+I A + GA+ K D T+ L + +
Sbjct: 139 WIRTYALFLEERLECYRVLKYDIEAERLPKGSGASSKNVDFNASQTYRTRMLSDEELLEQ 198
Query: 226 DPAFYQLYNRIVGYI---NAFKHYLKPYPQE---KLHF-VGVKINDVVVEKLVTFFDYSQ 384
PA QL R++G +A+ +YL Y K F + IND ++ + FF+ S+
Sbjct: 199 LPALQQLLYRLIGCQPEGSAYSNYLIQYALALVLKESFKIYCAINDGIINLVDMFFEMSR 258
Query: 385 FDATNSVFLTKK 420
DA ++ + K+
Sbjct: 259 HDAVKALNIYKR 270
>At5g57200.1 68418.m07145 epsin N-terminal homology (ENTH)
domain-containing protein / clathrin assembly
protein-related low similarity to clathrin assembly
protein AP180 [Xenopus laevis] GI:6492344; contains Pfam
profile PF01417: ENTH domain
Length = 591
Score = 29.1 bits (62), Expect = 2.7
Identities = 35/127 (27%), Positives = 56/127 (44%), Gaps = 9/127 (7%)
Frame = +1
Query: 67 WPENADLYEEEVTKDYQ-RSYEIVARHVLGAAPKPFDKH-TFMPSALDFYQTALRDPAFY 240
W L+ EE + Y+ Y+I A + A+ H T M S D + PA
Sbjct: 139 WVRTYALFLEERLECYRVLKYDIEAERLPKASGAASKTHRTRMLSGEDLLEQL---PALQ 195
Query: 241 QLYNRIVGYIN---AFKHYLKPYPQE---KLHF-VGVKINDVVVEKLVTFFDYSQFDATN 399
QL R++G A+ +YL Y K F + IND ++ + FF+ S+ DA
Sbjct: 196 QLLYRLIGCQPEGAAYSNYLIQYALALVLKESFKIYCAINDGIINLVDMFFEMSRHDAVK 255
Query: 400 SVFLTKK 420
++ + K+
Sbjct: 256 ALNIYKR 262
>At3g61160.2 68416.m06845 shaggy-related protein kinase beta /
ASK-beta (ASK2) identical to shaggy-related protein
kinase beta SP:O23145 GI:2569931 from [Arabidopsis
thaliana]
Length = 438
Score = 28.3 bits (60), Expect = 4.8
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = +1
Query: 544 LGPKYNDXGFPITLEENWHKFY 609
+ P+YND FP + WHK +
Sbjct: 337 MNPRYNDFKFPQIKAQPWHKIF 358
>At3g61160.1 68416.m06844 shaggy-related protein kinase beta /
ASK-beta (ASK2) identical to shaggy-related protein
kinase beta SP:O23145 GI:2569931 from [Arabidopsis
thaliana]
Length = 431
Score = 28.3 bits (60), Expect = 4.8
Identities = 9/22 (40%), Positives = 13/22 (59%)
Frame = +1
Query: 544 LGPKYNDXGFPITLEENWHKFY 609
+ P+YND FP + WHK +
Sbjct: 330 MNPRYNDFKFPQIKAQPWHKIF 351
>At1g64290.1 68414.m07285 F-box protein-related contains TIGRFAM
TIGR01640 : F-box protein interaction domain;
Length = 364
Score = 28.3 bits (60), Expect = 4.8
Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 3/51 (5%)
Frame = -2
Query: 474 FESWXTNLEVVWVTSLNLFFGQEYTVSG--IKLAIVKECD*FLNDNI-IDF 331
+ESW NL ++ ++ +F Q Y V G ++ + V E N+ + IDF
Sbjct: 38 YESWFVNLNLLRTNRISGYFIQHYIVKGHELRTSFVHERSDLQNNGVSIDF 88
>At1g13260.1 68414.m01539 DNA-binding protein RAV1 (RAV1) identical
to SP|Q9ZWM9 DNA-binding protein RAV1 {Arabidopsis
thaliana}, RAV1 GI:3868857 from [Arabidopsis thaliana]
Length = 344
Score = 27.9 bits (59), Expect = 6.3
Identities = 21/75 (28%), Positives = 35/75 (46%)
Frame = +1
Query: 193 SALDFYQTALRDPAFYQLYNRIVGYINAFKHYLKPYPQEKLHFVGVKINDVVVEKLVTFF 372
SA ++ A+ +L ++ +A KH+ P P + GV +N V V F
Sbjct: 183 SAEALFEKAVTPSDVGKLNRLVIPKHHAEKHF--PLPSSNVSVKGVLLNFEDVNGKVWRF 240
Query: 373 DYSQFDATNSVFLTK 417
YS ++++ S LTK
Sbjct: 241 RYSYWNSSQSYVLTK 255
>At5g63580.1 68418.m07981 flavonol synthase, putative similar to
SP|Q96330; contains PF03171 2OG-Fe(II) oxygenase
superfamily
Length = 250
Score = 27.5 bits (58), Expect = 8.4
Identities = 13/36 (36%), Positives = 22/36 (61%)
Frame = +1
Query: 46 INFVGNYWPENADLYEEEVTKDYQRSYEIVARHVLG 153
INF +YWP+N Y EV ++Y + + ++ +LG
Sbjct: 117 INF--DYWPKNPPQY-REVIEEYTKETKKLSERILG 149
>At3g54930.1 68416.m06087 serine/threonine protein phosphatase 2A
(PP2A) regulatory subunit B', putative similar to
SWISS-PROT:Q28653 serine/threonine protein phosphatase
2A, 56 kDa regulatory subunit, delta isoform (PP2A, B
subunit, B' delta isoform, PP2A, B subunit, B56 delta
isoform, PP2A, B subunit, PR61 delta isoform, PP2A, B
subunit, R5 delta isoform, PP2A, B subunit, B'-gamma)
[Oryctolagus cuniculus]; contains Pfam domain, PF01603:
Protein phosphatase 2A regulatory B subunit (B56 family)
Length = 497
Score = 27.5 bits (58), Expect = 8.4
Identities = 19/68 (27%), Positives = 31/68 (45%), Gaps = 5/68 (7%)
Frame = +1
Query: 28 FHDEKAINFVGNYWPENADLYEEEVTKDYQRSYEIVARHVLGAAPKP-----FDKHTFMP 192
+H+ GN PE + Y E Q YE++ R+V+ + +P F HTF+
Sbjct: 162 YHENTGAPPEGND-PEEEEPYLEPWWPHLQLVYELLLRYVVSSEIEPKTAKKFINHTFVS 220
Query: 193 SALDFYQT 216
LD + +
Sbjct: 221 RLLDLFDS 228
>At2g45870.1 68415.m05705 expressed protein contains Pfam profile
PF05249: Uncharacterised protein family (UPF0187)
Length = 410
Score = 27.5 bits (58), Expect = 8.4
Identities = 23/71 (32%), Positives = 36/71 (50%)
Frame = +2
Query: 11 MARKLISTMKRQLTLSETIGQRTPICMKKKLQRIINDLMKLSLAMCSVQHLNHSTSTPSC 190
++ KLIS +K S+ I +R + K+ L N + S S++HL H +S+PS
Sbjct: 55 LSEKLISLLKAVPNWSDGIKERR-MQQKRSLYTHENWVRHRS----SLRHLRHVSSSPSS 109
Query: 191 PVRLTFTKPHF 223
V L+ P F
Sbjct: 110 RVILSLIPPVF 120
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,799,945
Number of Sequences: 28952
Number of extensions: 275201
Number of successful extensions: 776
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 757
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 776
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1383534864
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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