Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_C08
(478 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 54 5e-08
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 54 5e-08
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 52 3e-07
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 52 3e-07
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 40 0.001
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 40 0.001
At5g20410.1 68418.m02427 1,2-diacylglycerol 3-beta-galactosyltra... 28 3.8
At2g12320.1 68415.m01329 hypothetical protein 27 5.0
At4g09780.1 68417.m01607 meprin and TRAF homology domain-contain... 27 8.7
At3g44050.1 68416.m04718 kinesin motor protein-related KLP2 prot... 27 8.7
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 54.0 bits (124), Expect = 5e-08
Identities = 27/76 (35%), Positives = 43/76 (56%), Gaps = 1/76 (1%)
Frame = +1
Query: 52 PCIQGTMDPPWRLTTREYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKE 231
P +G PW + E+ D +LY I G+++WQVK+G+IF+N L DDPA A+
Sbjct: 239 PNYKGKWKNPW-IDNPEFEDDPDLYVLKSIKYAGIEVWQVKAGSIFDNILICDDPAYARS 297
Query: 232 -KGEIIKKMQEGEKKM 276
+ + +E EK++
Sbjct: 298 IVDDYFAQHRESEKEL 313
Score = 37.9 bits (84), Expect = 0.004
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAYKGPWIHP 82
I N YKG W K+I NP YKG W +P
Sbjct: 222 IPNSAYKGPWKAKRIKNPNYKGKWKNP 248
Score = 33.1 bits (72), Expect = 0.100
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +2
Query: 23 GVWAPKQIDNPAYKGPW 73
G+W P +I N AYKGPW
Sbjct: 215 GLWEPPKIPNSAYKGPW 231
Score = 29.9 bits (64), Expect = 0.93
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAYK 64
I NP+YKG W IDNP ++
Sbjct: 236 IKNPNYKGKWKNPWIDNPEFE 256
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 54.0 bits (124), Expect = 5e-08
Identities = 27/76 (35%), Positives = 43/76 (56%), Gaps = 1/76 (1%)
Frame = +1
Query: 52 PCIQGTMDPPWRLTTREYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKE 231
P +G PW + E+ D +LY I G+++WQVK+G+IF+N L DDPA A+
Sbjct: 293 PNYKGKWKNPW-IDNPEFEDDPDLYVLKSIKYAGIEVWQVKAGSIFDNILICDDPAYARS 351
Query: 232 -KGEIIKKMQEGEKKM 276
+ + +E EK++
Sbjct: 352 IVDDYFAQHRESEKEL 367
Score = 37.9 bits (84), Expect = 0.004
Identities = 15/27 (55%), Positives = 17/27 (62%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAYKGPWIHP 82
I N YKG W K+I NP YKG W +P
Sbjct: 276 IPNSAYKGPWKAKRIKNPNYKGKWKNP 302
Score = 33.1 bits (72), Expect = 0.100
Identities = 11/17 (64%), Positives = 13/17 (76%)
Frame = +2
Query: 23 GVWAPKQIDNPAYKGPW 73
G+W P +I N AYKGPW
Sbjct: 269 GLWEPPKIPNSAYKGPW 285
Score = 29.9 bits (64), Expect = 0.93
Identities = 11/21 (52%), Positives = 14/21 (66%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAYK 64
I NP+YKG W IDNP ++
Sbjct: 290 IKNPNYKGKWKNPWIDNPEFE 310
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 51.6 bits (118), Expect = 3e-07
Identities = 25/63 (39%), Positives = 37/63 (58%)
Frame = +1
Query: 52 PCIQGTMDPPWRLTTREYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKE 231
P +G P + E+ D LY ++ VG++LWQVKSG++F+N L +DDP AK+
Sbjct: 287 PAYKGKWKAPM-IDNPEFKDDPELYVFPKLKYVGVELWQVKSGSLFDNVLVSDDPEYAKK 345
Query: 232 KGE 240
E
Sbjct: 346 LAE 348
Score = 45.2 bits (102), Expect = 2e-05
Identities = 17/27 (62%), Positives = 19/27 (70%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAYKGPWIHP 82
I NP+Y G W PK+I NPAYKG W P
Sbjct: 270 IPNPEYNGEWKPKKIKNPAYKGKWKAP 296
Score = 31.5 bits (68), Expect = 0.31
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAYK 64
I NP YKG W IDNP +K
Sbjct: 284 IKNPAYKGKWKAPMIDNPEFK 304
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 51.6 bits (118), Expect = 3e-07
Identities = 28/74 (37%), Positives = 43/74 (58%), Gaps = 1/74 (1%)
Frame = +1
Query: 52 PCIQGTMDPPWRLTTREYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKE 231
P +G + P + ++ D LY ++ VGL+LWQVKSG++F+N L DDP AK+
Sbjct: 287 PNYKGKWEAPL-IDNPDFKDDPELYVFPKLKYVGLELWQVKSGSLFDNVLICDDPDYAKK 345
Query: 232 -KGEIIKKMQEGEK 270
E K+++ EK
Sbjct: 346 LADETWGKLKDAEK 359
Score = 44.0 bits (99), Expect = 5e-05
Identities = 17/27 (62%), Positives = 18/27 (66%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAYKGPWIHP 82
I NP+Y G W PKQI NP YKG W P
Sbjct: 270 IPNPEYMGEWKPKQIKNPNYKGKWEAP 296
Score = 31.5 bits (68), Expect = 0.31
Identities = 12/21 (57%), Positives = 14/21 (66%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAYK 64
I NP+YKG W IDNP +K
Sbjct: 284 IKNPNYKGKWEAPLIDNPDFK 304
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 39.5 bits (88), Expect = 0.001
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +2
Query: 8 NPDYKGVWAPKQIDNPAYKGPW 73
NP YKG W+ IDNPAYKG W
Sbjct: 323 NPAYKGKWSSPLIDNPAYKGIW 344
Score = 37.1 bits (82), Expect = 0.006
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAY 61
IDNP YKG+W P+ I NP Y
Sbjct: 335 IDNPAYKGIWKPRDIPNPDY 354
Score = 30.7 bits (66), Expect = 0.53
Identities = 9/32 (28%), Positives = 21/32 (65%)
Frame = +1
Query: 133 DEICSVGLDLWQVKSGTIFNNFLFTDDPALAK 228
+ I ++G+++W ++ G +F+N L D +A+
Sbjct: 363 EPIAAIGIEIWTMQDGILFDNILIAKDEKVAE 394
Score = 27.1 bits (57), Expect = 6.6
Identities = 14/32 (43%), Positives = 15/32 (46%), Gaps = 5/32 (15%)
Frame = +2
Query: 2 IDNPDYK-----GVWAPKQIDNPAYKGPWIHP 82
IDNP + G W NPAYKG W P
Sbjct: 302 IDNPKCEAAPGCGEWKRPMKRNPAYKGKWSSP 333
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 39.5 bits (88), Expect = 0.001
Identities = 15/22 (68%), Positives = 16/22 (72%)
Frame = +2
Query: 8 NPDYKGVWAPKQIDNPAYKGPW 73
NP YKG W+ IDNPAYKG W
Sbjct: 325 NPAYKGKWSSPLIDNPAYKGIW 346
Score = 37.1 bits (82), Expect = 0.006
Identities = 13/20 (65%), Positives = 15/20 (75%)
Frame = +2
Query: 2 IDNPDYKGVWAPKQIDNPAY 61
IDNP YKG+W P+ I NP Y
Sbjct: 337 IDNPAYKGIWKPRDIPNPDY 356
Score = 31.1 bits (67), Expect = 0.40
Identities = 9/32 (28%), Positives = 22/32 (68%)
Frame = +1
Query: 133 DEICSVGLDLWQVKSGTIFNNFLFTDDPALAK 228
+ I ++G+++W ++ G +F+N L + D +A+
Sbjct: 365 EPIAAIGIEIWTMQDGILFDNILISKDEKVAE 396
>At5g20410.1 68418.m02427 1,2-diacylglycerol
3-beta-galactosyltransferase, putative /
monogalactosyldiacylglycerol synthase, putative / MGDG
synthase, putative identical to
monogalactosyldiacylglycerol synthase [gi:3367638] from
Arabidopsis thaliana, similar to MGDG synthase type A
[gi:9884651] from Glycine max
Length = 468
Score = 27.9 bits (59), Expect = 3.8
Identities = 11/37 (29%), Positives = 19/37 (51%)
Frame = -2
Query: 156 ETDRAYLITLVQVRVWSVFTGCQSPGWIHGPLYAGLS 46
+ +R+Y + V++W V SP WIH A ++
Sbjct: 119 DMERSYKFMVKHVQLWKVAFHSTSPKWIHSCYLAAIA 155
>At2g12320.1 68415.m01329 hypothetical protein
Length = 202
Score = 27.5 bits (58), Expect = 5.0
Identities = 9/20 (45%), Positives = 15/20 (75%)
Frame = -1
Query: 157 RDRQSISHHACTGSSLECIH 98
+D + HH+ +GSS+EC+H
Sbjct: 58 KDITTALHHSTSGSSVECLH 77
>At4g09780.1 68417.m01607 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein low similarity
to ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 443
Score = 26.6 bits (56), Expect = 8.7
Identities = 15/62 (24%), Positives = 28/62 (45%)
Frame = +1
Query: 88 LTTREYTPDSNLYKRDEICSVGLDLWQVKSGTIFNNFLFTDDPALAKEKGEIIKKMQEGE 267
L ++ N Y + C VG++++ +K +FT +P K +I + G+
Sbjct: 184 LLRSKFNDPKNGYTDGDACIVGVEIFVIKPIEKVERVVFTQNPPTNKFTWKISDFSKLGD 243
Query: 268 KK 273
KK
Sbjct: 244 KK 245
>At3g44050.1 68416.m04718 kinesin motor protein-related KLP2
protein, Xenopus laevis, PIR:T30335
Length = 1229
Score = 26.6 bits (56), Expect = 8.7
Identities = 9/17 (52%), Positives = 16/17 (94%)
Frame = +1
Query: 220 LAKEKGEIIKKMQEGEK 270
L KEKG+++K+++EGE+
Sbjct: 580 LQKEKGDLMKEIEEGER 596
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,357,426
Number of Sequences: 28952
Number of extensions: 141557
Number of successful extensions: 470
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 446
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 470
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 821630280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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