BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_B12 (488 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |S... 28 0.87 SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non ca... 28 0.87 SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pom... 26 2.7 SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr ... 26 3.5 SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ub... 25 4.6 SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharo... 25 4.6 SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo... 25 4.6 SPAC23H3.08c |bub3||mitotic spindle checkpoint protein Bub3|Schi... 25 6.1 SPAC12B10.13 |||CTLH domain|Schizosaccharomyces pombe|chr 1|||Ma... 25 6.1 SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D |Schi... 25 8.1 SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pomb... 25 8.1 SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosacchar... 25 8.1 >SPBC29A10.10c |||tRNA-splicing endonuclease positive effector |Schizosaccharomyces pombe|chr 2|||Manual Length = 1944 Score = 27.9 bits (59), Expect = 0.87 Identities = 24/61 (39%), Positives = 31/61 (50%), Gaps = 3/61 (4%) Frame = -2 Query: 181 VRASLSDSDVI*VLTDCCRMAV---RAYAHAPRVTNSHTLMNFILFYVRINDVRKRHVYR 11 VR+S+S I L D R+ V RA + V NS LM +FY I D + R V+R Sbjct: 1721 VRSSMSGG--IGFLQDLRRLNVALTRAKSSLYIVGNSKPLMQEDIFYSLIEDAKTRGVWR 1778 Query: 10 D 8 D Sbjct: 1779 D 1779 >SPBC1215.02c |arm1|mdm20|NatB N-acetyltransferase complex non catalytic subunit Arm1|Schizosaccharomyces pombe|chr 2|||Manual Length = 811 Score = 27.9 bits (59), Expect = 0.87 Identities = 17/63 (26%), Positives = 29/63 (46%), Gaps = 3/63 (4%) Frame = -3 Query: 378 RSSNVYPMIDCSFYPMSSLELYRNLLCRTSQSLSIGLR---LVQNSESAKFRHQLDDQRW 208 R++ YP S Y SSL++Y + T + +S+ Q + FR +LD W Sbjct: 480 RATTYYPSSVTSHYINSSLKIYGSNEFETPEMISMAYEDGAYSQIEDMRNFRSRLDHSTW 539 Query: 207 RML 199 + + Sbjct: 540 KSI 542 >SPBPJ4664.05 |||conserved fungal protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 163 Score = 26.2 bits (55), Expect = 2.7 Identities = 21/60 (35%), Positives = 31/60 (51%), Gaps = 1/60 (1%) Frame = -3 Query: 411 PLPLHPD*SRFRSSNVYPMIDCSFY-PMSSLELYRNLLCRTSQSLSIGLRLVQNSESAKF 235 PLPL+ D S VY +ID F+ P SL + +LL S ++S L + + + KF Sbjct: 56 PLPLNFDISVHLMPTVYTLIDYLFFSPPFSLSIGPSLLVYLSIAVSYMLWVEKCYQMNKF 115 >SPBC2G5.03 |||ATP binding protein|Schizosaccharomyces pombe|chr 2|||Manual Length = 335 Score = 25.8 bits (54), Expect = 3.5 Identities = 12/32 (37%), Positives = 18/32 (56%) Frame = -1 Query: 458 PRSTELVIEADSQDGSRSRFILTSLASDPVMY 363 PRSTE+ ++DS RS+ S + V+Y Sbjct: 191 PRSTEITTQSDSSPTKRSKPFKYSYEKEIVLY 222 >SPBC713.02c |ubp21|ubpD, ubp15|ubiquitin C-terminal hydrolase Ubp21|Schizosaccharomyces pombe|chr 2|||Manual Length = 1129 Score = 25.4 bits (53), Expect = 4.6 Identities = 13/42 (30%), Positives = 19/42 (45%) Frame = +3 Query: 282 DFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAG 407 D F D +L TT KRN WD+ + ++D +G Sbjct: 606 DMTDFSASDDDPVLITTKIKRNANIWDLQKHLAGLLNRDTSG 647 >SPAC1687.20c |mis6||inner centromere protein Mis6|Schizosaccharomyces pombe|chr 1|||Manual Length = 672 Score = 25.4 bits (53), Expect = 4.6 Identities = 9/17 (52%), Positives = 13/17 (76%) Frame = +1 Query: 121 PYGSNLLILKSHHYRIR 171 PY SNLL+L + HY ++ Sbjct: 148 PYISNLLVLLTKHYHVK 164 >SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizosaccharomyces pombe|chr 3|||Manual Length = 1045 Score = 25.4 bits (53), Expect = 4.6 Identities = 9/20 (45%), Positives = 14/20 (70%) Frame = -3 Query: 369 NVYPMIDCSFYPMSSLELYR 310 N+YP+ID S Y +S+ Y+ Sbjct: 954 NIYPVIDDSIYELSAASKYQ 973 >SPAC23H3.08c |bub3||mitotic spindle checkpoint protein Bub3|Schizosaccharomyces pombe|chr 1|||Manual Length = 320 Score = 25.0 bits (52), Expect = 6.1 Identities = 17/64 (26%), Positives = 28/64 (43%), Gaps = 2/64 (3%) Frame = +3 Query: 180 TKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGK--FYKGDSYIILKTTSDKRNNL 353 +K R+ F + +W ++ +PV + +D GK F IL +R NL Sbjct: 102 SKLRLENCFISGSWDKSFRVWDVRVKQPV--EGQDIGKKIFASSSRDNILVLGCSERENL 159 Query: 354 SWDI 365 +DI Sbjct: 160 VYDI 163 >SPAC12B10.13 |||CTLH domain|Schizosaccharomyces pombe|chr 1|||Manual Length = 240 Score = 25.0 bits (52), Expect = 6.1 Identities = 15/43 (34%), Positives = 22/43 (51%) Frame = -3 Query: 381 FRSSNVYPMIDCSFYPMSSLELYRNLLCRTSQSLSIGLRLVQN 253 F N+ P+ + ++SLEL +LLC S S L+ V N Sbjct: 140 FAHENLAPLAPSNQKFLNSLELTMSLLCFPPSSYSPALKNVLN 182 >SPBC11C11.04c |alp1||tubulin specific chaperone cofactor D |Schizosaccharomyces pombe|chr 2|||Manual Length = 1107 Score = 24.6 bits (51), Expect = 8.1 Identities = 10/18 (55%), Positives = 14/18 (77%) Frame = +1 Query: 187 LEFIQHSPTLVVKLVSKF 240 L+F Q PTL+ KL+SK+ Sbjct: 50 LQFCQFQPTLLDKLLSKY 67 >SPAC22G7.02 |kap111||karyopherin Kap111|Schizosaccharomyces pombe|chr 1|||Manual Length = 990 Score = 24.6 bits (51), Expect = 8.1 Identities = 9/15 (60%), Positives = 12/15 (80%) Frame = +3 Query: 129 QQSVNTQITSLSDKD 173 Q +NTQ+ +LSDKD Sbjct: 66 QMKINTQLETLSDKD 80 >SPBC1198.07c |||mannan endo-1,6-alpha-mannosidase |Schizosaccharomyces pombe|chr 2|||Manual Length = 507 Score = 24.6 bits (51), Expect = 8.1 Identities = 10/25 (40%), Positives = 14/25 (56%) Frame = +3 Query: 345 NNLSWDIHYWIGSETSQDEAGAAAI 419 NN +WD HY +G + S E A + Sbjct: 420 NNGTWDDHYGLGEQISAVETFQALL 444 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,133,315 Number of Sequences: 5004 Number of extensions: 42896 Number of successful extensions: 149 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 145 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 149 length of database: 2,362,478 effective HSP length: 68 effective length of database: 2,022,206 effective search space used: 190087364 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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