Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_B12
(488 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g57320.1 68418.m07160 villin, putative similar to villin 2 (V... 129 9e-31
At4g30160.1 68417.m04289 villin, putative similar to villin 2 (... 129 9e-31
At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to vil... 128 2e-30
At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to vil... 128 3e-30
At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to vil... 79 2e-15
At2g37660.1 68415.m04619 expressed protein 29 1.7
At4g01810.1 68417.m00238 protein transport protein-related relat... 29 2.2
At5g09450.1 68418.m01094 pentatricopeptide (PPR) repeat-containi... 28 3.9
At3g17380.1 68416.m02221 meprin and TRAF homology domain-contain... 27 6.8
At1g68130.1 68414.m07782 zinc finger (C2H2 type) family protein ... 27 6.8
>At5g57320.1 68418.m07160 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 962
Score = 129 bits (312), Expect = 9e-31
Identities = 54/96 (56%), Positives = 72/96 (75%)
Frame = +3
Query: 201 AFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIG 380
A AG+++G+EIWRI+NF+PV V + GKF+ GDSYI+LKTT+ + +L DIHYW+G
Sbjct: 11 ALQGAGQKSGIEIWRIENFKPVTVPQESHGKFFTGDSYIVLKTTASRSGSLHHDIHYWLG 70
Query: 381 SETSQDEAGAAAILTVGLDDQFGGTGVQHREAMGHE 488
++SQDEAGA A++TV LD GG VQ+RE GHE
Sbjct: 71 KDSSQDEAGAVAVMTVELDSALGGRAVQYREVQGHE 106
Score = 37.1 bits (82), Expect = 0.006
Identities = 24/102 (23%), Positives = 49/102 (48%), Gaps = 2/102 (1%)
Frame = +3
Query: 123 IRQQSVNTQITSLSDKDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYK 302
+++Q VN Q + + +K P G +++WRI E + +++ + KFY
Sbjct: 364 LQRQGVNVQ-GLVKTSSSSSKDEPKPYIDGTGN---LQVWRINCEEKILLEAAEQSKFYS 419
Query: 303 GDSYIILKT--TSDKRNNLSWDIHYWIGSETSQDEAGAAAIL 422
GD YI+ + D+ +L + W G ++ +++ +A L
Sbjct: 420 GDCYILQYSYPGEDREEHL---VGTWFGKQSVEEDRASAISL 458
>At4g30160.1 68417.m04289 villin, putative similar to villin 2
(VLN2) [Arabidopsis thaliana] GI:3415115, villin 3
(VLN3) [Arabidopsis thaliana] GI:3415117; contains Pfam
profiles PF00626: Gelsolin repeat, PF02209: Villin
headpiece domain
Length = 974
Score = 129 bits (312), Expect = 9e-31
Identities = 57/97 (58%), Positives = 69/97 (71%)
Frame = +3
Query: 198 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 377
PAF AG++AG+EIWRI+NF P + GKF+ GDSYI+LKTT+ K L DIHYW+
Sbjct: 10 PAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWL 69
Query: 378 GSETSQDEAGAAAILTVGLDDQFGGTGVQHREAMGHE 488
G +TSQDEAG AA+ TV LD GG VQ+RE GHE
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREVQGHE 106
Score = 37.5 bits (83), Expect = 0.005
Identities = 20/86 (23%), Positives = 37/86 (43%)
Frame = +3
Query: 231 VEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGA 410
+++WR+ +Q+ D KFY GD Y+ + + I W G ++ ++E G+
Sbjct: 394 LQVWRVNGQAKTLLQAADHSKFYSGDCYVFQYSYPGEEKE-EVLIGTWFGKQSVEEERGS 452
Query: 411 AAILTVGLDDQFGGTGVQHREAMGHE 488
A + + + Q R G E
Sbjct: 453 AVSMASKMVESMKFVPAQARIYEGKE 478
>At2g41740.1 68415.m05159 villin 2 (VLN2) nearly identical to villin
2 (VLN2) [Arabidopsis thaliana] GI:3415115
Length = 976
Score = 128 bits (309), Expect = 2e-30
Identities = 57/97 (58%), Positives = 69/97 (71%)
Frame = +3
Query: 198 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 377
PAF AG++ G EIWRI+NFE V V + GKFY GD+YI+L+TT +K +DIH+WI
Sbjct: 8 PAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 67
Query: 378 GSETSQDEAGAAAILTVGLDDQFGGTGVQHREAMGHE 488
G +TSQDEAG AA+ TV LD GG VQHRE GHE
Sbjct: 68 GKDTSQDEAGTAAVKTVELDAVLGGRAVQHREIQGHE 104
Score = 43.6 bits (98), Expect = 7e-05
Identities = 25/107 (23%), Positives = 43/107 (40%)
Frame = +3
Query: 168 KDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRN 347
K A + P + G+ +E+W + + +D GK Y GD Y++L T
Sbjct: 374 KSAPVNEDIPPLLESGGK---LEVWYVNGKVKTPLPKEDIGKLYSGDCYLVLYTYHSGER 430
Query: 348 NLSWDIHYWIGSETSQDEAGAAAILTVGLDDQFGGTGVQHREAMGHE 488
+ + W G ++ ++ A L + + G VQ R G E
Sbjct: 431 KDEYFLSCWFGKKSIPEDQDTAIRLANTMSNSLKGRPVQGRIYEGKE 477
>At3g57410.1 68416.m06391 villin 3 (VLN3) nearly identical to villin
3 (VLN3) [Arabidopsis thaliana] GI:3415117
Length = 965
Score = 128 bits (308), Expect = 3e-30
Identities = 56/97 (57%), Positives = 69/97 (71%)
Frame = +3
Query: 198 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSWDIHYWI 377
PAF G++ G EIWRI+NFEPV V + GKFY GD+YI+L+TT +K +DIH+WI
Sbjct: 10 PAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWI 69
Query: 378 GSETSQDEAGAAAILTVGLDDQFGGTGVQHREAMGHE 488
G +TSQDEAG AA+ TV LD GG VQ+RE GHE
Sbjct: 70 GKDTSQDEAGTAAVKTVELDAALGGRAVQYREIQGHE 106
Score = 42.7 bits (96), Expect = 1e-04
Identities = 30/122 (24%), Positives = 48/122 (39%)
Frame = +3
Query: 123 IRQQSVNTQITSLSDKDARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYK 302
++QQ V + LS K + P G+ +E+W I + GK Y
Sbjct: 364 LKQQGVG--LKGLS-KSTPVNEDIPPLLEGGGK---LEVWYIDANSKTVLSKDHVGKLYS 417
Query: 303 GDSYIILKTTSDKRNNLSWDIHYWIGSETSQDEAGAAAILTVGLDDQFGGTGVQHREAMG 482
GD Y++L T + + W G ++Q++ A L + + G VQ R G
Sbjct: 418 GDCYLVLYTYHSGERKEDYFLCCWFGKNSNQEDQETAVRLASTMTNSLKGRPVQARIFEG 477
Query: 483 HE 488
E
Sbjct: 478 KE 479
>At2g29890.1 68415.m03630 villin 1 (VLN1) nearly identical to villin
1 (VLN1) [Arabidopsis thaliana] GI:3415113
Length = 909
Score = 79.0 bits (186), Expect = 2e-15
Identities = 34/105 (32%), Positives = 61/105 (58%)
Frame = +3
Query: 174 ARTKARVHPAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYIILKTTSDKRNNL 353
+R + AF G ++G+EIW + N + +++ FGKF+ G++Y++L+T K +
Sbjct: 2 SRLSKDIDSAFQGVGTKSGLEIWCVYNKQLISIPKSSFGKFHSGNAYLVLRTFLRKIESP 61
Query: 354 SWDIHYWIGSETSQDEAGAAAILTVGLDDQFGGTGVQHREAMGHE 488
+DIHYW+G + ++ ++ A+ + LD G VQ+RE G E
Sbjct: 62 QYDIHYWLGIDANEVDSILASDKALDLDAALGCCTVQYREVQGQE 106
Score = 30.3 bits (65), Expect = 0.73
Identities = 20/73 (27%), Positives = 35/73 (47%), Gaps = 2/73 (2%)
Frame = +3
Query: 198 PAFANAGRQAGVEIWRIQNFEPVAVQSKDFGKFYKGDSYII-LKTTSDKRNNLSWDIHYW 374
P + N + +++WR+ + + D K + GD Y++ K T +R ++ W
Sbjct: 382 PLYTNC--RDNLKVWRVDGDDVSLLSIPDQTKLFTGDCYLVQYKYTYKERTEHL--LYVW 437
Query: 375 IGSET-SQDEAGA 410
IG E+ QD A A
Sbjct: 438 IGCESIQQDRADA 450
>At2g37660.1 68415.m04619 expressed protein
Length = 325
Score = 29.1 bits (62), Expect = 1.7
Identities = 12/32 (37%), Positives = 16/32 (50%)
Frame = +3
Query: 372 WIGSETSQDEAGAAAILTVGLDDQFGGTGVQH 467
WIG + D A AA + + L GGT + H
Sbjct: 182 WIGQKNQIDAAKAAGVKQIVLVGSMGGTNINH 213
>At4g01810.1 68417.m00238 protein transport protein-related related
to Sec23 protein [Homo sapiens] gi|1296664|emb|CAA65774
Length = 880
Score = 28.7 bits (61), Expect = 2.2
Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 2/56 (3%)
Frame = +3
Query: 261 PVAVQSKDFGKFYKGDSYIILKTTSDKRNNLSW--DIHYWIGSETSQDEAGAAAIL 422
P + ++ G F + +Y L SDK L D+ W+G+E S DE +AA+L
Sbjct: 733 PRCLMHQEGGTFEELPAYD-LSMQSDKAVILDHGTDVFIWLGAELSADEVKSAAVL 787
>At5g09450.1 68418.m01094 pentatricopeptide (PPR) repeat-containing
protein low similiarity to DNA-binding protein [Triticum
aestivum] GI:6958202; contains Pfam profile: PF01535 PPR
repeat
Length = 409
Score = 27.9 bits (59), Expect = 3.9
Identities = 10/33 (30%), Positives = 22/33 (66%)
Frame = -3
Query: 333 MSSLELYRNLLCRTSQSLSIGLRLVQNSESAKF 235
M++ L+ +L CR + + +G ++N+ES++F
Sbjct: 1 MATRSLFHSLRCRLTNNGVLGSNFIRNAESSRF 33
>At3g17380.1 68416.m02221 meprin and TRAF homology domain-containing
protein / MATH domain-containing protein similar to
ubiquitin-specific protease 12 [Arabidopsis thaliana]
GI:11993471; contains Pfam profile PF00917: MATH domain
Length = 309
Score = 27.1 bits (57), Expect = 6.8
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +3
Query: 237 IWRIQNFEPVAVQSKDFGKFYKGD 308
+W+I+NF + +S D F+ GD
Sbjct: 177 VWKIENFSKLDKESYDSNAFFAGD 200
>At1g68130.1 68414.m07782 zinc finger (C2H2 type) family protein
contains Pfam domain, PF00096: Zinc finger, C2H2 type
Length = 419
Score = 27.1 bits (57), Expect = 6.8
Identities = 14/33 (42%), Positives = 17/33 (51%)
Frame = -1
Query: 152 DLSINRLLPYGSARVRTRPKGHQQPHTYEFHFV 54
DLSI +LP + R P QQP T + FV
Sbjct: 232 DLSIGPILPGHPLQRRQSPPSEQQPSTLLYPFV 264
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,276,977
Number of Sequences: 28952
Number of extensions: 229814
Number of successful extensions: 661
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 661
length of database: 12,070,560
effective HSP length: 76
effective length of database: 9,870,208
effective search space used: 848837888
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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