BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_B03
(469 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosacch... 83 2e-17
SPAC27F1.05c |||aminotransferase class-III, unknown specificty|S... 30 0.20
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 4.4
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 5.8
SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos... 25 5.8
SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|c... 25 7.6
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 7.6
SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subuni... 25 7.6
>SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 474
Score = 83.4 bits (197), Expect = 2e-17
Identities = 43/96 (44%), Positives = 61/96 (63%), Gaps = 2/96 (2%)
Frame = +1
Query: 22 LKVIKQEKLIDLVNETGQFLKGGLHKLENEFPGLIHSVRGRG--TFLAYNAPNTETRDKI 195
L+ I+ + L++ V G FL GL +L + PG I+++RG+G TF+A++ + RDK
Sbjct: 372 LQEIQDKDLLNNVKSVGDFLYAGLEELARKHPGKINNLRGKGKGTFIAWDCESPAARDKF 431
Query: 196 NNKMKQNGVLGGTCGEVAIRLRPALIFQKKHAEIYL 303
M+ NGV G CG AIRLRP L+FQK HA+I L
Sbjct: 432 CADMRINGVNIGGCGVAAIRLRPMLVFQKHHAQILL 467
>SPAC27F1.05c |||aminotransferase class-III, unknown
specificty|Schizosaccharomyces pombe|chr 1|||Manual
Length = 484
Score = 29.9 bits (64), Expect = 0.20
Identities = 14/39 (35%), Positives = 22/39 (56%)
Frame = +1
Query: 31 IKQEKLIDLVNETGQFLKGGLHKLENEFPGLIHSVRGRG 147
I Q L+ + G + L+KL+ +FP ++ VRGRG
Sbjct: 358 IVQNDLLSRCRKLGGIMFDRLNKLQTKFPHVMKDVRGRG 396
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 25.4 bits (53), Expect = 4.4
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = -3
Query: 266 AGRRRIATSPQVPPSTPF 213
A RI++SP +PPS P+
Sbjct: 967 AAASRISSSPNMPPSNPY 984
>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1016
Score = 25.0 bits (52), Expect = 5.8
Identities = 10/28 (35%), Positives = 18/28 (64%)
Frame = +1
Query: 163 NAPNTETRDKINNKMKQNGVLGGTCGEV 246
+ P+TE+R +N + Q+G+L T G +
Sbjct: 193 DVPSTESRGNLNARRPQSGLLKITVGSL 220
>SPBP35G2.05c |cki2||serine/threonine protein kinase
Cki2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 435
Score = 25.0 bits (52), Expect = 5.8
Identities = 9/17 (52%), Positives = 13/17 (76%)
Frame = -2
Query: 288 MLLLEYKGWSKTNSHFT 238
MLL + KGW ++SHF+
Sbjct: 296 MLLNDGKGWESSSSHFS 312
>SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|chr
2|||Manual
Length = 680
Score = 24.6 bits (51), Expect = 7.6
Identities = 14/51 (27%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
Frame = +1
Query: 91 LHK-LENEFPGLIHSVRGRGTF-LAYNAPNTETRDKINNKMKQNGVLGGTC 237
LH+ + N F GL S +G GTF ++ + R + + ++ + G C
Sbjct: 191 LHQFIRNNFSGLESSTKGNGTFTVSKTTRKNQPRSRRDPRLSWKALGGEYC 241
>SPAC1805.15c |pub2||ubiquitin-protein ligase
Pub2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 671
Score = 24.6 bits (51), Expect = 7.6
Identities = 11/27 (40%), Positives = 16/27 (59%)
Frame = +1
Query: 22 LKVIKQEKLIDLVNETGQFLKGGLHKL 102
LK + + KL+ E LKGGL++L
Sbjct: 506 LKALTEHKLVTSTEEQFNALKGGLNEL 532
>SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subunit
Cwg2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 355
Score = 24.6 bits (51), Expect = 7.6
Identities = 10/23 (43%), Positives = 12/23 (52%)
Frame = +1
Query: 73 QFLKGGLHKLENEFPGLIHSVRG 141
Q GG K EFP ++HS G
Sbjct: 294 QHALGGFSKTPGEFPDVLHSALG 316
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,652,509
Number of Sequences: 5004
Number of extensions: 30767
Number of successful extensions: 79
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 78
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 178394480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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