BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0002_B03 (469 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosacch... 83 2e-17 SPAC27F1.05c |||aminotransferase class-III, unknown specificty|S... 30 0.20 SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 25 4.4 SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom... 25 5.8 SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos... 25 5.8 SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|c... 25 7.6 SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo... 25 7.6 SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subuni... 25 7.6 >SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosaccharomyces pombe|chr 1|||Manual Length = 474 Score = 83.4 bits (197), Expect = 2e-17 Identities = 43/96 (44%), Positives = 61/96 (63%), Gaps = 2/96 (2%) Frame = +1 Query: 22 LKVIKQEKLIDLVNETGQFLKGGLHKLENEFPGLIHSVRGRG--TFLAYNAPNTETRDKI 195 L+ I+ + L++ V G FL GL +L + PG I+++RG+G TF+A++ + RDK Sbjct: 372 LQEIQDKDLLNNVKSVGDFLYAGLEELARKHPGKINNLRGKGKGTFIAWDCESPAARDKF 431 Query: 196 NNKMKQNGVLGGTCGEVAIRLRPALIFQKKHAEIYL 303 M+ NGV G CG AIRLRP L+FQK HA+I L Sbjct: 432 CADMRINGVNIGGCGVAAIRLRPMLVFQKHHAQILL 467 >SPAC27F1.05c |||aminotransferase class-III, unknown specificty|Schizosaccharomyces pombe|chr 1|||Manual Length = 484 Score = 29.9 bits (64), Expect = 0.20 Identities = 14/39 (35%), Positives = 22/39 (56%) Frame = +1 Query: 31 IKQEKLIDLVNETGQFLKGGLHKLENEFPGLIHSVRGRG 147 I Q L+ + G + L+KL+ +FP ++ VRGRG Sbjct: 358 IVQNDLLSRCRKLGGIMFDRLNKLQTKFPHVMKDVRGRG 396 >SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizosaccharomyces pombe|chr 2|||Manual Length = 1224 Score = 25.4 bits (53), Expect = 4.4 Identities = 9/18 (50%), Positives = 13/18 (72%) Frame = -3 Query: 266 AGRRRIATSPQVPPSTPF 213 A RI++SP +PPS P+ Sbjct: 967 AAASRISSSPNMPPSNPY 984 >SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharomyces pombe|chr 2|||Manual Length = 1016 Score = 25.0 bits (52), Expect = 5.8 Identities = 10/28 (35%), Positives = 18/28 (64%) Frame = +1 Query: 163 NAPNTETRDKINNKMKQNGVLGGTCGEV 246 + P+TE+R +N + Q+G+L T G + Sbjct: 193 DVPSTESRGNLNARRPQSGLLKITVGSL 220 >SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizosaccharomyces pombe|chr 2|||Manual Length = 435 Score = 25.0 bits (52), Expect = 5.8 Identities = 9/17 (52%), Positives = 13/17 (76%) Frame = -2 Query: 288 MLLLEYKGWSKTNSHFT 238 MLL + KGW ++SHF+ Sbjct: 296 MLLNDGKGWESSSSHFS 312 >SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|chr 2|||Manual Length = 680 Score = 24.6 bits (51), Expect = 7.6 Identities = 14/51 (27%), Positives = 25/51 (49%), Gaps = 2/51 (3%) Frame = +1 Query: 91 LHK-LENEFPGLIHSVRGRGTF-LAYNAPNTETRDKINNKMKQNGVLGGTC 237 LH+ + N F GL S +G GTF ++ + R + + ++ + G C Sbjct: 191 LHQFIRNNFSGLESSTKGNGTFTVSKTTRKNQPRSRRDPRLSWKALGGEYC 241 >SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharomyces pombe|chr 1|||Manual Length = 671 Score = 24.6 bits (51), Expect = 7.6 Identities = 11/27 (40%), Positives = 16/27 (59%) Frame = +1 Query: 22 LKVIKQEKLIDLVNETGQFLKGGLHKL 102 LK + + KL+ E LKGGL++L Sbjct: 506 LKALTEHKLVTSTEEQFNALKGGLNEL 532 >SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subunit Cwg2|Schizosaccharomyces pombe|chr 1|||Manual Length = 355 Score = 24.6 bits (51), Expect = 7.6 Identities = 10/23 (43%), Positives = 12/23 (52%) Frame = +1 Query: 73 QFLKGGLHKLENEFPGLIHSVRG 141 Q GG K EFP ++HS G Sbjct: 294 QHALGGFSKTPGEFPDVLHSALG 316 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 1,652,509 Number of Sequences: 5004 Number of extensions: 30767 Number of successful extensions: 79 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 77 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 78 length of database: 2,362,478 effective HSP length: 67 effective length of database: 2,027,210 effective search space used: 178394480 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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