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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0002_B03
         (469 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase |Schizosacch...    83   2e-17
SPAC27F1.05c |||aminotransferase class-III, unknown specificty|S...    30   0.20 
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo...    25   4.4  
SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C |Schizosaccharom...    25   5.8  
SPBP35G2.05c |cki2||serine/threonine protein kinase Cki2|Schizos...    25   5.8  
SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|c...    25   7.6  
SPAC1805.15c |pub2||ubiquitin-protein ligase Pub2|Schizosaccharo...    25   7.6  
SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subuni...    25   7.6  

>SPAC19D5.07 |uga1||4-aminobutyrate aminotransferase
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 474

 Score = 83.4 bits (197), Expect = 2e-17
 Identities = 43/96 (44%), Positives = 61/96 (63%), Gaps = 2/96 (2%)
 Frame = +1

Query: 22  LKVIKQEKLIDLVNETGQFLKGGLHKLENEFPGLIHSVRGRG--TFLAYNAPNTETRDKI 195
           L+ I+ + L++ V   G FL  GL +L  + PG I+++RG+G  TF+A++  +   RDK 
Sbjct: 372 LQEIQDKDLLNNVKSVGDFLYAGLEELARKHPGKINNLRGKGKGTFIAWDCESPAARDKF 431

Query: 196 NNKMKQNGVLGGTCGEVAIRLRPALIFQKKHAEIYL 303
              M+ NGV  G CG  AIRLRP L+FQK HA+I L
Sbjct: 432 CADMRINGVNIGGCGVAAIRLRPMLVFQKHHAQILL 467


>SPAC27F1.05c |||aminotransferase class-III, unknown
           specificty|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 484

 Score = 29.9 bits (64), Expect = 0.20
 Identities = 14/39 (35%), Positives = 22/39 (56%)
 Frame = +1

Query: 31  IKQEKLIDLVNETGQFLKGGLHKLENEFPGLIHSVRGRG 147
           I Q  L+    + G  +   L+KL+ +FP ++  VRGRG
Sbjct: 358 IVQNDLLSRCRKLGGIMFDRLNKLQTKFPHVMKDVRGRG 396


>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
            |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1224

 Score = 25.4 bits (53), Expect = 4.4
 Identities = 9/18 (50%), Positives = 13/18 (72%)
 Frame = -3

Query: 266  AGRRRIATSPQVPPSTPF 213
            A   RI++SP +PPS P+
Sbjct: 967  AAASRISSSPNMPPSNPY 984


>SPBC12D12.04c |pck2|sts6, pkc1|protein kinase C
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 1016

 Score = 25.0 bits (52), Expect = 5.8
 Identities = 10/28 (35%), Positives = 18/28 (64%)
 Frame = +1

Query: 163 NAPNTETRDKINNKMKQNGVLGGTCGEV 246
           + P+TE+R  +N +  Q+G+L  T G +
Sbjct: 193 DVPSTESRGNLNARRPQSGLLKITVGSL 220


>SPBP35G2.05c |cki2||serine/threonine protein kinase
           Cki2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 435

 Score = 25.0 bits (52), Expect = 5.8
 Identities = 9/17 (52%), Positives = 13/17 (76%)
 Frame = -2

Query: 288 MLLLEYKGWSKTNSHFT 238
           MLL + KGW  ++SHF+
Sbjct: 296 MLLNDGKGWESSSSHFS 312


>SPBC1A4.09 |||pseudouridine synthase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 680

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 14/51 (27%), Positives = 25/51 (49%), Gaps = 2/51 (3%)
 Frame = +1

Query: 91  LHK-LENEFPGLIHSVRGRGTF-LAYNAPNTETRDKINNKMKQNGVLGGTC 237
           LH+ + N F GL  S +G GTF ++      + R + + ++    + G  C
Sbjct: 191 LHQFIRNNFSGLESSTKGNGTFTVSKTTRKNQPRSRRDPRLSWKALGGEYC 241


>SPAC1805.15c |pub2||ubiquitin-protein ligase
           Pub2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 671

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 11/27 (40%), Positives = 16/27 (59%)
 Frame = +1

Query: 22  LKVIKQEKLIDLVNETGQFLKGGLHKL 102
           LK + + KL+    E    LKGGL++L
Sbjct: 506 LKALTEHKLVTSTEEQFNALKGGLNEL 532


>SPAC2E1P5.04c |cwg2|orb7|geranylgeranyltransferase I beta subunit
           Cwg2|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 355

 Score = 24.6 bits (51), Expect = 7.6
 Identities = 10/23 (43%), Positives = 12/23 (52%)
 Frame = +1

Query: 73  QFLKGGLHKLENEFPGLIHSVRG 141
           Q   GG  K   EFP ++HS  G
Sbjct: 294 QHALGGFSKTPGEFPDVLHSALG 316


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,652,509
Number of Sequences: 5004
Number of extensions: 30767
Number of successful extensions: 79
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 77
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 78
length of database: 2,362,478
effective HSP length: 67
effective length of database: 2,027,210
effective search space used: 178394480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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