Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_B01
(317 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g35630.1 68417.m05060 phosphoserine aminotransferase, chlorop... 116 3e-27
At2g17630.1 68415.m02039 phosphoserine aminotransferase, putativ... 105 5e-24
At3g07020.2 68416.m00834 UDP-glucose:sterol glucosyltransferase ... 27 2.7
At3g07020.1 68416.m00833 UDP-glucose:sterol glucosyltransferase ... 27 2.7
At1g51910.1 68414.m05851 protein kinase family protein contains ... 27 2.7
At5g56110.1 68418.m07000 myb family transcription factor contain... 26 4.8
At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N domain-... 26 4.8
At2g45940.1 68415.m05712 hypothetical protein contains Pfam prof... 26 6.3
At1g70200.1 68414.m08078 RNA recognition motif (RRM)-containing ... 26 6.3
At1g67720.1 68414.m07728 leucine-rich repeat family protein / pr... 26 6.3
At1g04700.1 68414.m00467 protein kinase family protein low simil... 26 6.3
At5g24350.1 68418.m02870 expressed protein weak similarity to ne... 25 8.3
At4g19190.1 68417.m02832 zinc knuckle (CCHC-type) family protein... 25 8.3
At3g61590.1 68416.m06898 F-box family protein contains weak hit ... 25 8.3
At3g58160.1 68416.m06485 myosin heavy chain, putative similar to... 25 8.3
At2g32590.1 68415.m03979 barren family protein low similarity to... 25 8.3
At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putat... 25 8.3
>At4g35630.1 68417.m05060 phosphoserine aminotransferase,
chloroplast (PSAT) identical to Phosphoserine
aminotransferase, chloroplast precursor (PSAT)
(SP:Q96255)[Arabidopsis thaliana]; contains TIGRFAM
TIGR01364: phosphoserine aminotransferase; contains Pfam
PF00266: aminotransferase, class V
Length = 430
Score = 116 bits (280), Expect = 3e-27
Identities = 55/89 (61%), Positives = 66/89 (74%)
Frame = +1
Query: 22 TDRHVDIPDQSTWNLDPNASYVHICANETIHGVEFDFIPDTKGVPLVADMSSNFMSKKVD 201
++++ +P P+A Y+HICANETIHGVEF P K LVADMSSNF SK VD
Sbjct: 193 SEKYTKVPSFEELEQTPDAKYLHICANETIHGVEFKDYPVPKNGFLVADMSSNFCSKPVD 252
Query: 202 VSKFGVIYGGAQKNIGTSGVALVIVREDL 288
VSKFGVIYGGAQKN+G SGV +VI+R+DL
Sbjct: 253 VSKFGVIYGGAQKNVGPSGVTIVIIRKDL 281
>At2g17630.1 68415.m02039 phosphoserine aminotransferase, putative
similar to Phosphoserine aminotransferase, chloroplast
precursor (PSAT) (SP:Q96255) [Arabidopsis thaliana];
contains TIGRFAM TIGR01364: phosphoserine
aminotransferase; contains Pfam PF00266:
aminotransferase, class V
Length = 422
Score = 105 bits (253), Expect = 5e-24
Identities = 53/92 (57%), Positives = 66/92 (71%), Gaps = 3/92 (3%)
Frame = +1
Query: 22 TDRHVDIPDQSTWNLDPNASYVHICANETIHGVEFDFIP---DTKGVPLVADMSSNFMSK 192
++++ +P +A Y+HICANETIHGVEF P + GV L+ADMSSNF SK
Sbjct: 183 SEKYTKVPTFDGLEQSSDAKYLHICANETIHGVEFKDYPLVENPDGV-LIADMSSNFCSK 241
Query: 193 KVDVSKFGVIYGGAQKNIGTSGVALVIVREDL 288
VDVSKFGVIY GAQKN+G SGV +VI+R+DL
Sbjct: 242 PVDVSKFGVIYAGAQKNVGPSGVTIVIIRKDL 273
>At3g07020.2 68416.m00834 UDP-glucose:sterol glucosyltransferase
(UGT80A2) identical to UDP-glucose:sterol
glucosyltransferase [Arabidopsis thaliana] GI:2462931;
contains Pfam profile: PF03033 glycosyltransferase
family 28 N-terminal domain
Length = 637
Score = 27.1 bits (57), Expect = 2.7
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 64 LDPNASYVHICANETIHGVEFDFIPDTKGVPLV 162
+D S +CA+E+I GV+ +IP + V L+
Sbjct: 167 VDRGESKNGVCADESIDGVDLQYIPPMQIVMLI 199
>At3g07020.1 68416.m00833 UDP-glucose:sterol glucosyltransferase
(UGT80A2) identical to UDP-glucose:sterol
glucosyltransferase [Arabidopsis thaliana] GI:2462931;
contains Pfam profile: PF03033 glycosyltransferase
family 28 N-terminal domain
Length = 637
Score = 27.1 bits (57), Expect = 2.7
Identities = 12/33 (36%), Positives = 20/33 (60%)
Frame = +1
Query: 64 LDPNASYVHICANETIHGVEFDFIPDTKGVPLV 162
+D S +CA+E+I GV+ +IP + V L+
Sbjct: 167 VDRGESKNGVCADESIDGVDLQYIPPMQIVMLI 199
>At1g51910.1 68414.m05851 protein kinase family protein contains
Serine/Threonine protein kinases active-site signature,
PROSITE:PS00108
Length = 876
Score = 27.1 bits (57), Expect = 2.7
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = +1
Query: 148 GVPLVADMSSNFMSKKVDVSKFGVI 222
G P D +N +S+K DV FGV+
Sbjct: 737 GTPGYLDPETNLLSEKTDVYSFGVV 761
>At5g56110.1 68418.m07000 myb family transcription factor contains
PFAM profile: Myb DNA binding domain PF00249
Length = 320
Score = 26.2 bits (55), Expect = 4.8
Identities = 19/86 (22%), Positives = 37/86 (43%)
Frame = +1
Query: 22 TDRHVDIPDQSTWNLDPNASYVHICANETIHGVEFDFIPDTKGVPLVADMSSNFMSKKVD 201
T + VD+ + N +PN + H A+ ++ D + G+ + DM + F
Sbjct: 165 TKKRVDLNQINFSNHNPNPNNFHEIADNEAGKIKMDGLDHGNGIMKLWDMGNGFSYGSSS 224
Query: 202 VSKFGVIYGGAQKNIGTSGVALVIVR 279
S +G ++N G++ A+ R
Sbjct: 225 SS-----FGNEERNDGSASPAVAAWR 245
>At1g04850.1 68414.m00481 ubiquitin-associated (UBA)/TS-N
domain-containing protein weak similarity to SP|P45974
Ubiquitin carboxyl-terminal hydrolase 5 (EC 3.1.2.15)
{Homo sapiens}; contains Pfam profile PF00627: UBA/TS-N
domain
Length = 413
Score = 26.2 bits (55), Expect = 4.8
Identities = 12/47 (25%), Positives = 24/47 (51%)
Frame = +1
Query: 82 YVHICANETIHGVEFDFIPDTKGVPLVADMSSNFMSKKVDVSKFGVI 222
++ +C E I G EF F+P K + + + ++ ++ FGV+
Sbjct: 367 FMELCGFEKIEGGEFLFLPRDKIDSAIINSAGTELNSAINNPFFGVL 413
>At2g45940.1 68415.m05712 hypothetical protein contains Pfam profile
PF03478: Protein of unknown function (DUF295)
Length = 399
Score = 25.8 bits (54), Expect = 6.3
Identities = 14/49 (28%), Positives = 22/49 (44%)
Frame = +1
Query: 70 PNASYVHICANETIHGVEFDFIPDTKGVPLVADMSSNFMSKKVDVSKFG 216
P SY H C NE + GV P+ + M+ F ++V + + G
Sbjct: 131 PPRSYEHSCQNELVSGVAMSSSPEEEDFV----MAVKFTGRRVSIYRPG 175
>At1g70200.1 68414.m08078 RNA recognition motif (RRM)-containing
protein contains INTERPRO:IPR000504 RNA-binding region
RNP-1 (RNA recognition motif) domain
Length = 538
Score = 25.8 bits (54), Expect = 6.3
Identities = 13/33 (39%), Positives = 17/33 (51%), Gaps = 1/33 (3%)
Frame = -1
Query: 185 IKFDDISAT-SGTPFVSGIKSNSTPCMVSFAHI 90
+ F ++S S TP VSG K P FAH+
Sbjct: 206 VAFKEVSGVLSITPAVSGNKKTKDPVCKGFAHV 238
>At1g67720.1 68414.m07728 leucine-rich repeat family protein /
protein kinase family protein contains similarity to
light repressible receptor protein kinase [Arabidopsis
thaliana] gi|1321686|emb|CAA66376; contains Pfam doamins
PF00069: Protein kinase domain and PF00560: Leucine Rich
Repeat
Length = 929
Score = 25.8 bits (54), Expect = 6.3
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = +1
Query: 151 VPLVADMSSNFMSKKVDVSKFGVIYGGAQKN 243
+P++ + + NF SKKV FG +Y G K+
Sbjct: 598 LPVLEEATDNF-SKKVGRGSFGSVYYGRMKD 627
>At1g04700.1 68414.m00467 protein kinase family protein low similarity
to EDR1 [Arabidopsis thaliana] GI:11127925; contains Pfam
profile: PF00069 Eukaryotic protein kinase domain
Length = 1042
Score = 25.8 bits (54), Expect = 6.3
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +1
Query: 154 PLVADMSSNFMSKKVDVSKFGVI 222
P + + SSN +S+KVDV FG++
Sbjct: 942 PELLNGSSNRVSEKVDVFSFGIV 964
>At5g24350.1 68418.m02870 expressed protein weak similarity to
neuroblastoma-amplified protein [Homo sapiens] GI:4337460
Length = 2376
Score = 25.4 bits (53), Expect = 8.3
Identities = 9/18 (50%), Positives = 12/18 (66%)
Frame = +1
Query: 7 MVLPATDRHVDIPDQSTW 60
+VLP ++ IPD STW
Sbjct: 1741 LVLPPNGSYMHIPDSSTW 1758
>At4g19190.1 68417.m02832 zinc knuckle (CCHC-type) family protein
contains Pfam domain, PF00098: Zinc knuckle
Length = 595
Score = 25.4 bits (53), Expect = 8.3
Identities = 12/34 (35%), Positives = 17/34 (50%)
Frame = -2
Query: 106 FRLRIYVHMRH*DPSSTLTDPVYRHDDQSLVKPY 5
+RL H +H DPS++L VY + K Y
Sbjct: 382 YRLSRRRHTKHVDPSASLKSEVYHQGNSHREKHY 415
>At3g61590.1 68416.m06898 F-box family protein contains weak hit to
Pfam PF00646: F-box domain; stamina pistilloidia (Stp) -
Pisum sativum, EMBL:AF004843
Length = 411
Score = 25.4 bits (53), Expect = 8.3
Identities = 10/28 (35%), Positives = 20/28 (71%)
Frame = -1
Query: 182 KFDDISATSGTPFVSGIKSNSTPCMVSF 99
+FD++ A+SGT + I+S +P +++F
Sbjct: 347 EFDEVFASSGTDDLVYIQSYGSPALLTF 374
>At3g58160.1 68416.m06485 myosin heavy chain, putative similar to
myosin heavy chain [Arabidopsis thaliana]
gi|602328|emb|CAA84067.
Length = 1242
Score = 25.4 bits (53), Expect = 8.3
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = -1
Query: 194 FFDIKFDDISATSGTPFVSGIKSNSTPCMVS 102
F +I+FDD+ SG + + S C VS
Sbjct: 216 FVEIQFDDVGRISGAAIRTYLLERSRVCQVS 246
>At2g32590.1 68415.m03979 barren family protein low similarity to
SP|Q9Y7R3 Condensin complex subunit 2 (p105)
{Schizosaccharomyces pombe}; contains Pfam profile
PF05786: Barren protein
Length = 704
Score = 25.4 bits (53), Expect = 8.3
Identities = 12/46 (26%), Positives = 19/46 (41%)
Frame = -1
Query: 215 PNLDTSTFFDIKFDDISATSGTPFVSGIKSNSTPCMVSFAHICTYE 78
P LD + + + DI A P + ++ TPC C Y+
Sbjct: 475 PELDFAKALEEEMPDIFAPPKNPKTLLLPASRTPCQTKLPEDCHYQ 520
>At2g05710.1 68415.m00611 aconitate hydratase, cytoplasmic, putative
/ citrate hydro-lyase/aconitase, putative nearly
identical to SP|P49608 Aconitate hydratase, cytoplasmic
(EC 4.2.1.3) (Citrate hydro-lyase) (Aconitase)
{Cucurbita maxima}; contains Pfam profiles PF00330:
Aconitase family (aconitate hydratase), PF00694:
Aconitase C-terminal domain
Length = 990
Score = 25.4 bits (53), Expect = 8.3
Identities = 6/19 (31%), Positives = 15/19 (78%)
Frame = +1
Query: 34 VDIPDQSTWNLDPNASYVH 90
+ +P+ + ++ DPN++Y+H
Sbjct: 722 LSVPENTLYSWDPNSTYIH 740
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,216,014
Number of Sequences: 28952
Number of extensions: 143602
Number of successful extensions: 409
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 402
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 408
length of database: 12,070,560
effective HSP length: 71
effective length of database: 10,014,968
effective search space used: 340508912
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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