Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0002_A23
(558 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 44 5e-05
At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 44 9e-05
At3g02280.1 68416.m00209 flavodoxin family protein low similarit... 29 2.8
At1g70900.1 68414.m08181 expressed protein 29 2.8
At1g23110.1 68414.m02889 hypothetical protein 29 2.8
At5g19010.1 68418.m02258 mitogen-activated protein kinase, putat... 28 4.9
At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 28 4.9
At4g01290.1 68417.m00170 expressed protein 28 4.9
At3g25140.1 68416.m03139 glycosyl transferase family 8 protein c... 28 4.9
At5g35940.1 68418.m04325 jacalin lectin family protein similar t... 27 6.4
At4g30550.1 68417.m04336 glutamine amidotransferase class-I doma... 27 6.4
At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 27 6.4
At2g20170.1 68415.m02358 hypothetical protein and grail contain... 27 6.4
At5g59920.1 68418.m07514 DC1 domain-containing protein contains ... 27 8.5
At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identic... 27 8.5
At2g23990.2 68415.m02866 plastocyanin-like domain-containing pro... 27 8.5
At2g23990.1 68415.m02865 plastocyanin-like domain-containing pro... 27 8.5
>At3g45220.1 68416.m04880 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 393
Score = 44.4 bits (100), Expect = 5e-05
Identities = 37/137 (27%), Positives = 65/137 (47%), Gaps = 7/137 (5%)
Frame = +3
Query: 159 TSSKDKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKF--LGNGDY-SGVANPYISLSKTF 329
T + N+V SP+ + +L+ L AG+ ++ +I F L + DY + V +S++
Sbjct: 24 TVANGSNLVFSPMSINVLLCLIAAGSNCVTKEQILSFIMLPSSDYLNAVLAKTVSVALND 83
Query: 330 SEMNPD-YFTMANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTKKAADI--INQWA 497
D + + A +++ + F + Y + +DF+ TK A I +N WA
Sbjct: 84 GMERSDLHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFA-TKPAEVINEVNAWA 142
Query: 498 NEKTRGHIKSPISDDTI 548
T G IK +SDD+I
Sbjct: 143 EVHTNGLIKEILSDDSI 159
>At2g26390.1 68415.m03167 serpin, putative / serine protease
inhibitor, putative similar to phloem serpin-1
[Cucurbita maxima] GI:9937311; contains Pfam profile
PF00079: Serpin (serine protease inhibitor)
Length = 389
Score = 43.6 bits (98), Expect = 9e-05
Identities = 33/128 (25%), Positives = 60/128 (46%), Gaps = 3/128 (2%)
Frame = +3
Query: 177 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 356
NVV SP+ + +L+ L AG+ ++ EI FL + + ++ +E + +
Sbjct: 31 NVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDHLNAVLAKIADGGTERSDLCLS 90
Query: 357 MANKIYVGNKYTLDEKF-TITVRQYQSEVETIDFSDTK--KAADIINQWANEKTRGHIKS 527
A+ +++ L F + Y++ +DF+ TK + D +N WA+ T G IK
Sbjct: 91 TAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFA-TKPVEVIDEVNIWADVHTNGLIKQ 149
Query: 528 PISDDTID 551
+S D D
Sbjct: 150 ILSRDCTD 157
>At3g02280.1 68416.m00209 flavodoxin family protein low similarity
to SP|Q05001 NADPH-cytochrome P450 reductase (EC
1.6.2.4) {Catharanthus roseus}, similar to
NADPH-dependent FMN and FAD containing oxidoreductase
[Homo sapiens] GI:6694369; contains Pfam profiles
PF00258: flavodoxin, PF00667: FAD binding domain,
PF00175: Oxidoreductase NAD-binding domain
Length = 623
Score = 28.7 bits (61), Expect = 2.8
Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 7/80 (8%)
Frame = +3
Query: 243 GSRAEIDKFLGNGD----YSGVANPY-ISLSKTFSEMNPDYFTMANKIYVGNKYTLDE-K 404
G+ I+K LG+ Y G +P+ +SL +T ++NP YF + + +D+ K
Sbjct: 123 GATTIIEKGLGDDQHPSGYEGTLDPWMLSLWRTLYQINPKYFPKGPDVKIPQDEVIDKPK 182
Query: 405 FTITV-RQYQSEVETIDFSD 461
+ I +Q + E + + SD
Sbjct: 183 YRILFHKQEKLEPKLLSDSD 202
>At1g70900.1 68414.m08181 expressed protein
Length = 244
Score = 28.7 bits (61), Expect = 2.8
Identities = 19/66 (28%), Positives = 32/66 (48%)
Frame = +3
Query: 174 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 353
K +S +GV + LY + SR + K+L DY+ +A I L++ E NP +
Sbjct: 103 KVYANSLIGVGIASSLYHS-----SRGKFRKYLRWADYTMIATATICLTRALREENPKFL 157
Query: 354 TMANKI 371
A+ +
Sbjct: 158 MAASAL 163
>At1g23110.1 68414.m02889 hypothetical protein
Length = 261
Score = 28.7 bits (61), Expect = 2.8
Identities = 20/66 (30%), Positives = 32/66 (48%)
Frame = +3
Query: 174 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 353
K +S +GV + LY A SR ++ K+L DY+ +A I LS+ NP +
Sbjct: 120 KVYANSLIGVGIASSLYHA-----SRGKLRKYLRWVDYTMIATTTICLSRALRNENPKFL 174
Query: 354 TMANKI 371
A+ +
Sbjct: 175 MAASAL 180
>At5g19010.1 68418.m02258 mitogen-activated protein kinase, putative
/ MAPK, putative (MPK16) mitogen-activated protein
kinase (MAPK)(AtMPK16), PMID:12119167; similar to
ATMPK9, Arabidopsis thaliana, EMBL:AB038694
Length = 567
Score = 27.9 bits (59), Expect = 4.9
Identities = 11/24 (45%), Positives = 15/24 (62%)
Frame = +3
Query: 237 GEGSRAEIDKFLGNGDYSGVANPY 308
GEGSR I++ +G G Y V + Y
Sbjct: 20 GEGSRYRIEEVIGKGSYGVVCSAY 43
>At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase II SP:P27046 from [Mus
musculus]
Length = 1173
Score = 27.9 bits (59), Expect = 4.9
Identities = 10/29 (34%), Positives = 18/29 (62%)
Frame = -3
Query: 226 LYRSISIITPNGEDTTFLSFDDVYVSLRN 140
LYR+ +++ P G+D ++S D+ RN
Sbjct: 401 LYRTNTLLIPLGDDFRYISIDEAEAQFRN 429
>At4g01290.1 68417.m00170 expressed protein
Length = 991
Score = 27.9 bits (59), Expect = 4.9
Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 4/54 (7%)
Frame = +1
Query: 361 PIKSTSEINILWMRNSRSLSVSTKAKLKLLT----SAIPKKQLTSLISGLMRRP 510
P+ + + + M R SV +A LL+ S+ PK Q T L+S RRP
Sbjct: 584 PVDQDTSVPSVKMTKQRKTSVDDQASQHLLSLLQRSSDPKSQDTQLLSATERRP 637
>At3g25140.1 68416.m03139 glycosyl transferase family 8 protein
contains Pfam profile: PF01501 glycosyl transferase
family 8
Length = 559
Score = 27.9 bits (59), Expect = 4.9
Identities = 17/77 (22%), Positives = 35/77 (45%)
Frame = +3
Query: 177 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 356
+VV+ + + + +++K +G+ E+ DY+ + + Y+ + K N F
Sbjct: 281 HVVTDKMNLGAMQVMFKLKEYKGAHVEVKAV---EDYTFLNSSYVPVLKQLESANLQKFY 337
Query: 357 MANKIYVGNKYTLDEKF 407
NK+ K T + KF
Sbjct: 338 FENKLENATKDTTNMKF 354
>At5g35940.1 68418.m04325 jacalin lectin family protein similar to
myrosinase-binding protein homolog [Arabidopsis
thaliana] GI:2997767; contains Pfam profile PF01419
jacalin-like lectin domain
Length = 444
Score = 27.5 bits (58), Expect = 6.4
Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 1/61 (1%)
Frame = +3
Query: 171 DKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKT-FSEMNPD 347
DKNV S LG + +L +G + + GDY G+ Y++ T M D
Sbjct: 131 DKNVYS--LGAYFIKVLATKSGMQGGQNTGKSYDYGGDYDGIRKVYVTYDGTSIRHMRVD 188
Query: 348 Y 350
Y
Sbjct: 189 Y 189
>At4g30550.1 68417.m04336 glutamine amidotransferase class-I
domain-containing protein similar to defense-related
protein [Brassica carinata] GI:14009290, component of
aniline dioxygenase (GMP synthase like protein) -
Acinetobacter sp.,PID:d1013698; contains Pfam profile
PF00117: glutamine amidotransferase class-I
Length = 249
Score = 27.5 bits (58), Expect = 6.4
Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 8/60 (13%)
Frame = +3
Query: 36 LLAVIAVCNVRAFYSSDH--------EYNRTALGDAIDKTSLQFLKETYTSSKDKNVVSS 191
LLA VCNV F DH EYN+ L + +D+ L E + K K+ + +
Sbjct: 172 LLASSEVCNVEMFSIGDHFFCIQGHPEYNKEILFEIVDRVLNMKLMEQEFADKAKSTMET 231
>At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing
protein low similarity to nucleolar phosphoprotein
(Nopp52), Tetrahymena thermophila, EMBL:TT51555;
contains InterPro entry IPR000504: RNA-binding region
RNP-1 (RNA recognition motif) (RRM)
Length = 597
Score = 27.5 bits (58), Expect = 6.4
Identities = 13/41 (31%), Positives = 19/41 (46%)
Frame = +3
Query: 261 DKFLGNGDYSGVANPYISLSKTFSEMNPDYFTMANKIYVGN 383
D++L Y P I KT S P+ N++Y+GN
Sbjct: 228 DRYLTIQQYVKTTTPSIPRRKTSSGFAPEMVDGYNRVYIGN 268
>At2g20170.1 68415.m02358 hypothetical protein and grail contains
Pfam profile PF03080: Arabidopsis proteins of unknown
function
Length = 401
Score = 27.5 bits (58), Expect = 6.4
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = +3
Query: 99 RTALGDAIDKTSLQFLKETYTSSKDKNVVS 188
RT L D I L++L YT+SKDK+ ++
Sbjct: 121 RTTLEDLIQIQRLKYLGVKYTTSKDKDFLN 150
>At5g59920.1 68418.m07514 DC1 domain-containing protein contains
Pfam profile PF03107: DC1 domain
Length = 710
Score = 27.1 bits (57), Expect = 8.5
Identities = 15/41 (36%), Positives = 20/41 (48%)
Frame = +2
Query: 92 VQQDSLRRCH*QNFFAVS*GNVYIIEGQECCVFTIRRDDAN 214
V D RRC V G++Y+ E +ECC F + AN
Sbjct: 384 VIHDERRRCE-ACIHPVQFGSIYVCEEEECC-FVLHEKCAN 422
>At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identical
to AtSPO11-1 [Arabidopsis thaliana] GI:13383478;
contains Pfam profile PF04406: Type IIB DNA
topoisomerase; identical to cDNA putative topoisomerase
VIA (SPO11 gene 1) GI:7270974
Length = 362
Score = 27.1 bits (57), Expect = 8.5
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +3
Query: 114 DAIDKTSLQFLKETYTSSKDKNVVSSP 194
+A+ SL FL E Y SK + VSSP
Sbjct: 336 EALSVHSLSFLSEVYIPSKIRREVSSP 362
>At2g23990.2 68415.m02866 plastocyanin-like domain-containing
protein
Length = 226
Score = 27.1 bits (57), Expect = 8.5
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -3
Query: 283 SPLPKNLSISARLPSPAPALYRSISIITPNG 191
+P PK+ S S P+PAPA ++ ++ NG
Sbjct: 181 TPSPKS-STSTMAPAPAPAKSSAVGLVAGNG 210
>At2g23990.1 68415.m02865 plastocyanin-like domain-containing
protein
Length = 207
Score = 27.1 bits (57), Expect = 8.5
Identities = 12/31 (38%), Positives = 19/31 (61%)
Frame = -3
Query: 283 SPLPKNLSISARLPSPAPALYRSISIITPNG 191
+P PK+ S S P+PAPA ++ ++ NG
Sbjct: 162 TPSPKS-STSTMAPAPAPAKSSAVGLVAGNG 191
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,953,524
Number of Sequences: 28952
Number of extensions: 247833
Number of successful extensions: 787
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 768
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 787
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1062855648
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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