Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_O20
(657 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 321 6e-89
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 149 5e-37
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 144 1e-35
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 91 1e-19
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 29 0.78
SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces pomb... 28 1.0
SPBP4H10.04 |ppb1||calcineurin catalytic subunit Ppb1|Schizosacc... 28 1.0
SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein Ubr1|Sc... 26 4.2
SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyce... 25 7.3
SPAC11E3.11c |||guanyl-nucleotide exchange factor |Schizosacchar... 25 7.3
SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyce... 25 9.6
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 321 bits (788), Expect = 6e-89
Identities = 142/185 (76%), Positives = 168/185 (90%)
Frame = +2
Query: 2 LNADLRKLAVNMVPFPRLHFFITGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDP 181
LN+DLRKLAVNMVPFPRLHFF+ GFAPL + GS ++A++VPELTQQMFDA NMM A DP
Sbjct: 246 LNSDLRKLAVNMVPFPRLHFFMVGFAPLAAIGSSSFQAVSVPELTQQMFDANNMMVAADP 305
Query: 182 RHGRYLTVATIFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAA 361
RHGRYLTVA +FRG++SMKEVDEQ+ ++Q KNS+YFVEWIP+NV AVC +PP+ LKM+A
Sbjct: 306 RHGRYLTVAALFRGKVSMKEVDEQIRSVQTKNSAYFVEWIPDNVLKAVCSVPPKDLKMSA 365
Query: 362 TFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGKGMDEMEFTEAESNMNDLISEYQQY 541
TFIGNST+IQE+F+R+ +QF+AMFRRKAFLHWYTG+GMDEMEFTEAESNMNDL+SEYQQY
Sbjct: 366 TFIGNSTSIQEIFRRLGDQFSAMFRRKAFLHWYTGEGMDEMEFTEAESNMNDLVSEYQQY 425
Query: 542 QEAAI 556
QEA I
Sbjct: 426 QEAGI 430
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 149 bits (360), Expect = 5e-37
Identities = 65/187 (34%), Positives = 109/187 (58%), Gaps = 8/187 (4%)
Frame = +2
Query: 2 LNADLRKLAVNMVPFPRLHFFITGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDP 181
LN DL + N+VP+PR+HF + +AP+ S + + +V E+T Q F+ N M CDP
Sbjct: 248 LNVDLAEFQTNLVPYPRIHFPLVTYAPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP 307
Query: 182 RHGRYLTVATIFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLK--- 352
R GRY+ ++RG + ++V + I+ K + FV+W P K +CD PP+ ++
Sbjct: 308 RAGRYMATCLLYRGDVIPRDVQAAVTTIKAKRTIQFVDWCPTGFKIGICDRPPQHIEGSE 367
Query: 353 -----MAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGKGMDEMEFTEAESNMND 517
A + N+T+I E + R+ +F M+ ++AF+HWY G+GM+E EF+EA ++
Sbjct: 368 IAKVDRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAA 427
Query: 518 LISEYQQ 538
L +Y++
Sbjct: 428 LERDYEE 434
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 144 bits (348), Expect = 1e-35
Identities = 62/187 (33%), Positives = 109/187 (58%), Gaps = 8/187 (4%)
Frame = +2
Query: 2 LNADLRKLAVNMVPFPRLHFFITGFAPLTSRGSQQYRALTVPELTQQMFDAKNMMAACDP 181
LN DL + N+VP+PR+HF + ++P+ S + + +V E+T Q F+ N M CDP
Sbjct: 252 LNVDLNEFQTNLVPYPRIHFPLVTYSPIVSAAKAFHESNSVQEITNQCFEPYNQMVKCDP 311
Query: 182 RHGRYLTVATIFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPR------ 343
R GRY+ ++RG + ++V + +I+++ + FV+W P K +C PP+
Sbjct: 312 RTGRYMATCLLYRGDVIPRDVQAAVTSIKSRRTIQFVDWCPTGFKIGICYEPPQHVPGSG 371
Query: 344 --GLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGKGMDEMEFTEAESNMND 517
+ A + N+T+I E + R+ +F M+ ++AF+HWY G+GM+E EF+EA ++
Sbjct: 372 IAKVNRAVCMLSNTTSIAEAWSRLDHKFDLMYSKRAFVHWYVGEGMEEGEFSEAREDLAA 431
Query: 518 LISEYQQ 538
L +Y++
Sbjct: 432 LERDYEE 438
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 91.5 bits (217), Expect = 1e-19
Identities = 48/192 (25%), Positives = 105/192 (54%), Gaps = 10/192 (5%)
Frame = +2
Query: 2 LNADLRKLAVNMVPFPRLHFFITGFAPLTSRGSQQYRAL---TVPELTQQMFDAKNMMAA 172
+N DL + +++P PR HF +T + P T++ ++ +A+ TV ++ +++ KN M +
Sbjct: 249 MNNDLVSIIASLIPSPRCHFLLTSYTPFTNQQVEEAKAIRKTTVLDVMRRLLLPKNQMVS 308
Query: 173 CDP-RHGRYLTVATIFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPP--- 340
+P + ++++ I +G +V + ++ I+ + + F+ W P +++ A+ P
Sbjct: 309 VNPSKKSCFISILDIIQGEADPADVHKSLLRIRERRYASFIPWGPASIQVALSKKSPYIK 368
Query: 341 RGLKMAATFIGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGKGMDEMEFTEAESNMN-- 514
+++ + N T+I LFKR +Q+ + +R AFL Y + + E + E +S+ +
Sbjct: 369 TNHRVSGLMLANHTSIASLFKRTLDQYDRLRKRNAFLEQYKKEAIFEDDLNEFDSSRDVV 428
Query: 515 -DLISEYQQYQE 547
DLI+EY+ ++
Sbjct: 429 ADLINEYEACED 440
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 28.7 bits (61), Expect = 0.78
Identities = 14/36 (38%), Positives = 21/36 (58%)
Frame = +2
Query: 374 NSTAIQELFKRISEQFTAMFRRKAFLHWYTGKGMDE 481
NS+ IQ L K I+ T +R ++ Y+G G+DE
Sbjct: 193 NSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDE 228
>SPAC13C5.07 |rad32|mre11|Rad32 nuclease|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 649
Score = 28.3 bits (60), Expect = 1.0
Identities = 19/66 (28%), Positives = 30/66 (45%), Gaps = 6/66 (9%)
Frame = +3
Query: 438 ARHSCTGTPARAWTKWSSRRRRAT*TILYLNT------SSIKKRPSMKTPSSTRN*SKKR 599
+ H TP+ A K +++RR ++ NT +KK P+ K ST+ K
Sbjct: 562 SEHEMEATPSPALLKKTNKRRELPSSLTKKNTRTPQRSKEVKKVPARKLSQSTKKSDKNT 621
Query: 600 YTTNLF 617
+T LF
Sbjct: 622 QSTLLF 627
>SPBP4H10.04 |ppb1||calcineurin catalytic subunit
Ppb1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 554
Score = 28.3 bits (60), Expect = 1.0
Identities = 15/66 (22%), Positives = 33/66 (50%), Gaps = 2/66 (3%)
Frame = +2
Query: 212 IFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIG--NSTA 385
++ + ++ + + +MNI+ N S W+PN + +P G K++ I N +
Sbjct: 343 VYNNKAAVLKYENNVMNIRQFNCSPHPYWLPNFMDVFTWSLPFVGEKVSEMLISMLNICS 402
Query: 386 IQELFK 403
+EL++
Sbjct: 403 KEELYE 408
>SPBC19C7.02 |ubr1|SPBC32F12.14|N-end-recognizing protein
Ubr1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1958
Score = 26.2 bits (55), Expect = 4.2
Identities = 18/59 (30%), Positives = 29/59 (49%)
Frame = +2
Query: 368 IGNSTAIQELFKRISEQFTAMFRRKAFLHWYTGKGMDEMEFTEAESNMNDLISEYQQYQ 544
+ N + L+K + E+F+ +F RK L WY G+ E + N+N SE + Q
Sbjct: 1703 LNNPHLLFTLYKLL-ERFSLIFLRKCALLWYCRYGVS----FETQPNLNFQNSELSRLQ 1756
>SPBC19C7.12c |||alpha-1,2-mannosyltransferase|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 390
Score = 25.4 bits (53), Expect = 7.3
Identities = 11/39 (28%), Positives = 17/39 (43%)
Frame = +2
Query: 191 RYLTVATIFRGRMSMKEVDEQMMNIQNKNSSYFVEWIPN 307
+Y+ ++F G + NI N S EW+PN
Sbjct: 29 QYIPTISVFEGSLIDNRDTLSYFNISNLEPSERSEWLPN 67
>SPAC11E3.11c |||guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 942
Score = 25.4 bits (53), Expect = 7.3
Identities = 15/46 (32%), Positives = 23/46 (50%)
Frame = +3
Query: 474 WTKWSSRRRRAT*TILYLNTSSIKKRPSMKTPSSTRN*SKKRYTTN 611
W K S R +I +T +I +RPSM+ ++ SK +TN
Sbjct: 50 WFKNESSSRHPLTSIKENDTQTIGRRPSMRVKLFGKDKSKASMSTN 95
>SPAC3H5.08c |||WD repeat protein Wdr44 family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 855
Score = 25.0 bits (52), Expect = 9.6
Identities = 15/41 (36%), Positives = 25/41 (60%)
Frame = +2
Query: 251 QMMNIQNKNSSYFVEWIPNNVKTAVCDIPPRGLKMAATFIG 373
++ +I+ K S++ E +P + TAV P GL +A TF+G
Sbjct: 375 RLWSIKEKAVSFWNE-LPELI-TAVAFSPDGGLAIAGTFVG 413
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,202,199
Number of Sequences: 5004
Number of extensions: 38502
Number of successful extensions: 144
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 141
length of database: 2,362,478
effective HSP length: 70
effective length of database: 2,012,198
effective search space used: 297805304
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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