SilkBase IMG001 IMG002 IMG003 IMG005 IMG006 IMG007 IMG008 IMG009 kuwako IMG010 IMG011 IMG012

Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0001_O16
         (597 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p...   105   4e-24
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo...    75   1e-14
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ...    67   2e-12
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase...    58   1e-09
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d...    54   2e-08
SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe...    30   0.22 
SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces ...    26   3.6  
SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces...    25   6.3  
SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomy...    25   8.4  
SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3 |Schiz...    25   8.4  

>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 360

 Score =  105 bits (253), Expect = 4e-24
 Identities = 62/184 (33%), Positives = 93/184 (50%), Gaps = 5/184 (2%)
 Frame = +3

Query: 60  VNNYPLPKITKDTDVIVKVEYSGVCGTDLHIIQ----GEFPALKDRPFPLGHESSGIIHE 227
           + + P   +T D  V V ++ +G+CG+D+H  +    G+F  +  +P  LGHES+G++ E
Sbjct: 18  IEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDF--ILKKPMILGHESAGVVVE 75

Query: 228 VGTA-SIFKKGQKVAVDPNRACNLCEFCRSGNYQYCLTAGINSTIGIWKDGGWAQYVCVP 404
           VG   S  K G  VAV+P   C LC++CRSG Y  C      +T     DG    Y    
Sbjct: 76  VGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPPY--DGTLRTYYITT 133

Query: 405 QDQVYALPDGITTEQAGLCEPYSCVSHGFKRASPLSVGKKILIVGAGIIGNLWVTTLHHH 584
           +D    LP  I+ E+  L EP S   H   R + L  G ++L++G G +G L +     +
Sbjct: 134 EDFCTKLPKQISVEEGALFEPMSVAVHAMTRGN-LKCGSRVLVMGCGTVGLLMMAVAKAY 192

Query: 585 GHRD 596
           G  D
Sbjct: 193 GAID 196


>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
           Adh1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 350

 Score = 74.5 bits (175), Expect = 1e-14
 Identities = 46/153 (30%), Positives = 74/153 (48%), Gaps = 4/153 (2%)
 Frame = +3

Query: 99  DVIVKVEYSGVCGTDLHIIQGEFPALKDRPFPLGHESSGIIHEVGT-ASIFKKGQKVAVD 275
           +V+V ++Y+GVC TDLH +QG++P     P   GHE +G++ +VG   +  K G +V V 
Sbjct: 35  EVLVNIKYTGVCHTDLHALQGDWPLPAKMPLIGGHEGAGVVVKVGAGVTRLKIGDRVGVK 94

Query: 276 -PNRACNLCEFCRSGNYQYCLTAGINSTIGIWKDGGWAQYVCVPQDQVYALPDGITTEQA 452
             N +C  CE+C       C    ++   G   DG +  Y          +P+ +  E A
Sbjct: 95  WMNSSCGNCEYCMKAEETICPHIQLS---GYTVDGTFQHYCIANATHATIIPESVPLEVA 151

Query: 453 G--LCEPYSCVSHGFKRASPLSVGKKILIVGAG 545
              +C   +C  +   + S +  G+ I I GAG
Sbjct: 152 APIMCAGITC--YRALKESKVGPGEWICIPGAG 182


>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 423

 Score = 67.3 bits (157), Expect = 2e-12
 Identities = 40/110 (36%), Positives = 56/110 (50%), Gaps = 2/110 (1%)
 Frame = +3

Query: 15  MEAVLFDGKTSTLKYVNNYPLPKITKDTDVIVKVEYSGVC-GTDLHIIQGEFPALKDRPF 191
           M+A ++DG  +    +   P P IT   DVIVK     +C G+D HI  GE P ++    
Sbjct: 37  MKACVWDGPLNVK--IAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPGIEKGAI 94

Query: 192 PLGHESSGIIHEVGT-ASIFKKGQKVAVDPNRACNLCEFCRSGNYQYCLT 338
            LGHES GI+ E G   +  + G +V +  + AC  C FC+   Y  C T
Sbjct: 95  -LGHESCGIVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDT 143


>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 378

 Score = 58.0 bits (134), Expect = 1e-09
 Identities = 34/97 (35%), Positives = 46/97 (47%), Gaps = 1/97 (1%)
 Frame = +3

Query: 90  KDTDVIVKVEYSGVCGTDLHIIQGEFPALKDRPFPLGHESSGIIHEVGTASI-FKKGQKV 266
           K  +V VKV++S VC TD + + G  P     P  LGHE +GI+  +G   I  + G  V
Sbjct: 33  KAHEVRVKVDWSAVCHTDAYTLSGVDPE-GAFPIVLGHEGAGIVESIGEGVINVRPGDHV 91

Query: 267 AVDPNRACNLCEFCRSGNYQYCLTAGINSTIGIWKDG 377
            +     C  C+FCRSG    C         G+  DG
Sbjct: 92  ILLYTPECKECKFCRSGKTNLCSKIRETQGRGLMPDG 128


>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
           dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 380

 Score = 53.6 bits (123), Expect = 2e-08
 Identities = 31/94 (32%), Positives = 44/94 (46%), Gaps = 1/94 (1%)
 Frame = +3

Query: 99  DVIVKVEYSGVCGTDLHIIQGEFPALKDRPFPLGHESSGIIHEVG-TASIFKKGQKVAVD 275
           +V +K+  SGVC TD + + G+ P     P  LGHE +GI+  VG   +  + G  V   
Sbjct: 39  EVRIKIVNSGVCHTDAYTLSGKDPE-GLFPVILGHEGAGIVESVGPQVTTVQVGDPVIAL 97

Query: 276 PNRACNLCEFCRSGNYQYCLTAGINSTIGIWKDG 377
               C  C+FC+SG    C         G+  DG
Sbjct: 98  YTPECKTCKFCKSGKTNLCGRIRTTQGKGLMPDG 131


>SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 325

 Score = 30.3 bits (65), Expect = 0.22
 Identities = 19/71 (26%), Positives = 33/71 (46%), Gaps = 2/71 (2%)
 Frame = +3

Query: 351 STIGIWKDGGWAQYVCVPQDQVYALPDGIT-TEQAGLCEPYSCVSHGFKRASPLSVGKKI 527
           S +   KDG  A+Y  +P+     +P  ++ TE A +  P++       R         +
Sbjct: 94  SELSFTKDGTHAEYCIIPEKAAVRMPSNLSFTEAASVGVPFTTAYLALSRGETKG-SDIV 152

Query: 528 LIVGA-GIIGN 557
           L+VGA G +G+
Sbjct: 153 LVVGALGAVGS 163


>SPCC285.14 |||TRAPP complex subunit Trs130 |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1150

 Score = 26.2 bits (55), Expect = 3.6
 Identities = 13/26 (50%), Positives = 17/26 (65%)
 Frame = +1

Query: 49  PLNTSTIILYPRLQKTRMS*SKWNTL 126
           PL  + I+LY R Q TRM  ++WN L
Sbjct: 386 PLCCAKILLYARRQLTRM--ARWNNL 409


>SPBC16C6.13c |sec27||coatomer beta' subunit |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 796

 Score = 25.4 bits (53), Expect = 6.3
 Identities = 14/50 (28%), Positives = 24/50 (48%), Gaps = 4/50 (8%)
 Frame = +3

Query: 231 GTASIFKKGQKVA----VDPNRACNLCEFCRSGNYQYCLTAGINSTIGIW 368
           GT  ++  G  VA       +R  N   +  +G+  Y +TAG ++ I +W
Sbjct: 164 GTVKVWSFGSSVANFTLQAHDRGVNYVNYYPAGDKPYLITAGDDNLIKVW 213


>SPBC3D6.12 |||U3 snoRNA associted protein Dip2 |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 922

 Score = 25.0 bits (52), Expect = 8.4
 Identities = 9/22 (40%), Positives = 14/22 (63%)
 Frame = +3

Query: 306 CRSGNYQYCLTAGINSTIGIWK 371
           C S + + CLTAG  S + +W+
Sbjct: 222 CVSPDAKRCLTAGTGSDVKVWE 243


>SPCC790.02 |pep3|vps18, vps18|ubiquitin-protein ligase E3
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 900

 Score = 25.0 bits (52), Expect = 8.4
 Identities = 10/37 (27%), Positives = 22/37 (59%)
 Frame = +3

Query: 189 FPLGHESSGIIHEVGTASIFKKGQKVAVDPNRACNLC 299
           F +G ++S + HE+ T +   + + + ++PN +C  C
Sbjct: 805 FEIG-QASEVAHEIQTNAENMRNRYIVLEPNESCWHC 840


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,521,640
Number of Sequences: 5004
Number of extensions: 51919
Number of successful extensions: 171
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 160
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 162
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 260219058
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

- SilkBase 1999-2023 -