BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_O09 (354 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protei... 103 3e-23 At3g58130.1 68416.m06482 N-acetylglucosaminyl-phosphatidylinosit... 28 2.1 At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransfera... 27 3.6 At5g53340.1 68418.m06629 galactosyltransferase family protein co... 27 4.7 At5g53010.1 68418.m06584 calcium-transporting ATPase, putative 27 4.7 At4g35740.2 68417.m05073 DNA helicase (RECQl3) identical to DNA ... 26 6.3 At4g35740.1 68417.m05072 DNA helicase (RECQl3) identical to DNA ... 26 6.3 At5g65390.1 68418.m08224 arabinogalactan-protein (AGP7) 26 8.3 At5g03640.1 68418.m00323 protein kinase family protein contains ... 26 8.3 >At2g35040.1 68415.m04299 AICARFT/IMPCHase bienzyme family protein similar to SP|P12048 Bifunctional purine biosynthesis protein purH [Includes: Phosphoribosylaminoimidazolecarboxamide formyltransferase (EC 2.1.2.3) (AICAR transformylase); IMP cyclohydrolase (EC 3.5.4.10) (Inosinicase) (IMP synthetase) (ATIC)] {Bacillus subtilis}; contains Pfam profiles PF01808: AICARFT/IMPCHase bienzyme, PF02142: MGS-like domain Length = 596 Score = 103 bits (248), Expect = 3e-23 Identities = 54/109 (49%), Positives = 66/109 (60%), Gaps = 1/109 (0%) Frame = +3 Query: 24 ASNGKLALLSVSDKTGLLILGKALSEMGLQLVGSGGTATALRNAGLSVLDVSDITKAPEM 203 +S K AL+S+SDK L LG L E+G +V +GGTA+ L NAG+SV V +T PEM Sbjct: 68 SSGEKQALISLSDKRDLASLGNGLQELGYTIVSTGGTASTLENAGVSVTKVEKLTHFPEM 127 Query: 204 LGGRVKTLHPAVHAGILARLS-NTDQEDMKRQNFDLISVVVCNLYPFVQ 347 L GRVKTLHP +H GILAR E + VVV NLYPF + Sbjct: 128 LDGRVKTLHPNIHGGILARRDVEHHMEALNEHGIGTFDVVVVNLYPFYE 176 >At3g58130.1 68416.m06482 N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase-related similar to SP|Q9Y2B2 N-acetylglucosaminyl-phosphatidylinositol de-N-acetylase (EC 3.5.1.-) (Phosphatidylinositol-glycan biosynthesis, class L protein) (PIG-L) {Homo sapiens} Length = 257 Score = 27.9 bits (59), Expect = 2.1 Identities = 12/34 (35%), Positives = 18/34 (52%) Frame = -3 Query: 229 CKVFTRPPSISGALVISETSRTESPAFLSAVAVP 128 CK+F R SISGA ++ + + L +A P Sbjct: 19 CKIFFRATSISGAAILDDGKTPQKKNVLFVIAHP 52 >At3g03710.1 68416.m00375 polyribonucleotide nucleotidyltransferase, putative similar to polynucleotide phosphorylase GB:AAC50039 [Pisum sativum], identical to putative polynucleotide phosphorylase GB:AAF00646 [Arabidopsis thaliana] Length = 922 Score = 27.1 bits (57), Expect = 3.6 Identities = 16/58 (27%), Positives = 28/58 (48%), Gaps = 2/58 (3%) Frame = +3 Query: 15 ADMASNGKLALLSVSDKTGLLILGKALSEMGLQLVGSGG--TATALRNAGLSVLDVSD 182 A+MA LS+S L+++ K L+GSGG + + +G+ +D+ D Sbjct: 674 AEMAKCSPPPTLSLSKYAPLILIMKVHPSKVYSLIGSGGKKVKSIIEESGVEAIDMQD 731 >At5g53340.1 68418.m06629 galactosyltransferase family protein contains Pfam profile: PF01762 galactosyltransferase Length = 338 Score = 26.6 bits (56), Expect = 4.7 Identities = 12/38 (31%), Positives = 19/38 (50%) Frame = +3 Query: 183 ITKAPEMLGGRVKTLHPAVHAGILARLSNTDQEDMKRQ 296 ++K+P++ G P V GI+ L N + D RQ Sbjct: 94 VSKSPKLADGTETKKRPLVVIGIMTSLGNKKKRDAVRQ 131 >At5g53010.1 68418.m06584 calcium-transporting ATPase, putative Length = 1049 Score = 26.6 bits (56), Expect = 4.7 Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 1/61 (1%) Frame = +3 Query: 87 KALSEMGLQL-VGSGGTATALRNAGLSVLDVSDITKAPEMLGGRVKTLHPAVHAGILARL 263 K L E + L +G GGTA A N+ +LD + T ++ R +L+ V IL RL Sbjct: 797 KTLREADVSLAMGVGGTAAAKENSDFIILDDNFATIVKCIIWSR--SLYNNVQKSILFRL 854 Query: 264 S 266 + Sbjct: 855 T 855 >At4g35740.2 68417.m05073 DNA helicase (RECQl3) identical to DNA Helicase [Arabidopsis thaliana] GI:11121447; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 620 Score = 26.2 bits (55), Expect = 6.3 Identities = 9/23 (39%), Positives = 18/23 (78%) Frame = -2 Query: 296 LTLHILLIGVRKSSQYAGVNSRM 228 L++H+ IG+ ++ +AG+NS+M Sbjct: 179 LSVHLSSIGISSAAYHAGLNSKM 201 >At4g35740.1 68417.m05072 DNA helicase (RECQl3) identical to DNA Helicase [Arabidopsis thaliana] GI:11121447; contains Pfam profiles PF00270: DEAD/DEAH box helicase, PF00271: Helicase conserved C-terminal domain Length = 713 Score = 26.2 bits (55), Expect = 6.3 Identities = 9/23 (39%), Positives = 18/23 (78%) Frame = -2 Query: 296 LTLHILLIGVRKSSQYAGVNSRM 228 L++H+ IG+ ++ +AG+NS+M Sbjct: 272 LSVHLSSIGISSAAYHAGLNSKM 294 >At5g65390.1 68418.m08224 arabinogalactan-protein (AGP7) Length = 130 Score = 25.8 bits (54), Expect = 8.3 Identities = 13/33 (39%), Positives = 17/33 (51%) Frame = -3 Query: 217 TRPPSISGALVISETSRTESPAFLSAVAVPPLP 119 T PP++S A S S SP+ + A PP P Sbjct: 51 TPPPAVSPAPTSSPPSSAPSPSSDAPTASPPAP 83 >At5g03640.1 68418.m00323 protein kinase family protein contains serine/threonine protein kinase domain, INTERPRO:IPR002290 Length = 926 Score = 25.8 bits (54), Expect = 8.3 Identities = 12/41 (29%), Positives = 22/41 (53%) Frame = +3 Query: 36 KLALLSVSDKTGLLILGKALSEMGLQLVGSGGTATALRNAG 158 K+ LL + + ++ GK ++++ + GG ALRN G Sbjct: 255 KVRLLYANTPSSTIVNGKRVAKLTRTIPRGGGAKPALRNKG 295 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 7,307,323 Number of Sequences: 28952 Number of extensions: 135301 Number of successful extensions: 329 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 317 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 328 length of database: 12,070,560 effective HSP length: 72 effective length of database: 9,986,016 effective search space used: 449370720 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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