BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_O06 (680 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At3g45220.1 68416.m04880 serpin, putative / serine protease inhi... 59 2e-09 At2g26390.1 68415.m03167 serpin, putative / serine protease inhi... 58 5e-09 At2g25240.1 68415.m03020 serpin, putative / serine protease inhi... 55 4e-08 At1g64030.1 68414.m07252 serpin family protein / serine protease... 52 3e-07 At1g47710.1 68414.m05302 serpin, putative / serine protease inhi... 52 5e-07 At2g35580.1 68415.m04357 serpin family protein / serine protease... 40 0.002 At1g63280.1 68414.m07154 serpin-related / serine protease inhibi... 36 0.019 At1g62170.1 68414.m07013 serpin family protein / serine protease... 33 0.17 At1g51330.1 68414.m05772 serpin-related / serine protease inhibi... 33 0.23 At1g05960.1 68414.m00625 expressed protein similar to hypothetic... 29 2.2 At3g02280.1 68416.m00209 flavodoxin family protein low similarit... 29 3.8 At1g70900.1 68414.m08181 expressed protein 29 3.8 At1g23110.1 68414.m02889 hypothetical protein 29 3.8 At5g19010.1 68418.m02258 mitogen-activated protein kinase, putat... 28 6.6 At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 28 6.6 At4g01290.1 68417.m00170 expressed protein 28 6.6 At3g25140.1 68416.m03139 glycosyl transferase family 8 protein c... 28 6.6 At5g35940.1 68418.m04325 jacalin lectin family protein similar t... 27 8.7 At4g30550.1 68417.m04336 glutamine amidotransferase class-I doma... 27 8.7 At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing ... 27 8.7 At2g20170.1 68415.m02358 hypothetical protein and grail contain... 27 8.7 >At3g45220.1 68416.m04880 serpin, putative / serine protease inhibitor, putative similar to phloem serpin-1 [Cucurbita maxima] GI:9937311; contains Pfam profile PF00079: Serpin (serine protease inhibitor) Length = 393 Score = 59.3 bits (137), Expect = 2e-09 Identities = 47/170 (27%), Positives = 80/170 (47%), Gaps = 9/170 (5%) Frame = +3 Query: 147 TSSKDKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKF--LGNGDY-SGVANPYISLSKTF 317 T + N+V SP+ + +L+ L AG+ ++ +I F L + DY + V +S++ Sbjct: 24 TVANGSNLVFSPMSINVLLCLIAAGSNCVTKEQILSFIMLPSSDYLNAVLAKTVSVALND 83 Query: 318 SEMNPD-YFTMANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTKKAADI--INQWA 485 D + + A +++ + F + Y + +DF+ TK A I +N WA Sbjct: 84 GMERSDLHLSTAYGVWIDKSLSFKPSFKDLLENSYNATCNQVDFA-TKPAEVINEVNAWA 142 Query: 486 NEKTRGHIKSPISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETKEKD 629 T G IK +SDD+I + L N ++F+G W F+A TK D Sbjct: 143 EVHTNGLIKEILSDDSIKTIRESMLILANAVYFKGAWSKKFDAKLTKSYD 192 >At2g26390.1 68415.m03167 serpin, putative / serine protease inhibitor, putative similar to phloem serpin-1 [Cucurbita maxima] GI:9937311; contains Pfam profile PF00079: Serpin (serine protease inhibitor) Length = 389 Score = 58.0 bits (134), Expect = 5e-09 Identities = 43/163 (26%), Positives = 76/163 (46%), Gaps = 8/163 (4%) Frame = +3 Query: 165 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 344 NVV SP+ + +L+ L AG+ ++ EI FL + + ++ +E + + Sbjct: 31 NVVFSPMSINVLLSLIAAGSNPVTKEEILSFLMSPSTDHLNAVLAKIADGGTERSDLCLS 90 Query: 345 MANKIYVGNKYTLDEKF-TITVRQYQSEVETIDFSDTK--KAADIINQWANEKTRGHIKS 515 A+ +++ L F + Y++ +DF+ TK + D +N WA+ T G IK Sbjct: 91 TAHGVWIDKSSYLKPSFKELLENSYKASCSQVDFA-TKPVEVIDEVNIWADVHTNGLIKQ 149 Query: 516 PISDDTIDP-----NAAAALFNVIFFQGHWHVPFNASETKEKD 629 +S D D N+ L N ++F+ W F+A TK+ D Sbjct: 150 ILSRDCTDTIKEIRNSTLILANAVYFKAAWSRKFDAKLTKDND 192 >At2g25240.1 68415.m03020 serpin, putative / serine protease inhibitor, putative similar to phloem serpin-1 [Cucurbita maxima] GI:9937311; contains Pfam profile PF00079: Serpin (serine protease inhibitor) Length = 324 Score = 55.2 bits (127), Expect = 4e-08 Identities = 30/100 (30%), Positives = 53/100 (53%), Gaps = 4/100 (4%) Frame = +3 Query: 342 TMANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDF-SDTKKAADIINQWANEKTRGHIKS 515 ++AN +++ ++L F + Y++ +DF S + D +N WA T G IK Sbjct: 28 SIANGVWIDKFFSLKLSFKDLLENSYKATCSQVDFASKPSEVIDEVNTWAEVHTNGLIKQ 87 Query: 516 PISDDTIDP--NAAAALFNVIFFQGHWHVPFNASETKEKD 629 +S D+ID ++ L N ++F+G W F+A+ TK+ D Sbjct: 88 ILSRDSIDTIRSSTLVLANAVYFKGAWSSKFDANMTKKND 127 >At1g64030.1 68414.m07252 serpin family protein / serine protease inhibitor family protein similar to phloem serpin-1 [Cucurbita maxima] GI:9937311, serpin [Triticum aestivum] GI:871551; contains Pfam profile PF00079: Serpin (serine protease inhibitor) Length = 385 Score = 52.4 bits (120), Expect = 3e-07 Identities = 39/171 (22%), Positives = 68/171 (39%), Gaps = 7/171 (4%) Frame = +3 Query: 147 TSSKDKNVVSSPLGVMMLMLLYKAG-AGEGSRAEIDKFLGNGDYSGVANPYISLSKTF-- 317 ++ KD NV+ SP + + ++ AG G+ +I FL + + + L+ Sbjct: 24 SAPKDSNVIFSPASINSAITMHAAGPGGDLVSGQILSFLRSSSIDELKTVFRELASVVYA 83 Query: 318 --SEMNPDYFTMANKIYVGNKYTLDEKFTITVRQYQSEVET-IDF-SDTKKAADIINQWA 485 S T AN +++ D KF + V +DF S+ ++ +N W Sbjct: 84 DRSATGGPKITAANGLWIDKSLPTDPKFKDLFENFFKAVYVPVDFRSEAEEVRKEVNSWV 143 Query: 486 NEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKDSML 638 T IK + D ++ N + F+G W PF T++ D L Sbjct: 144 EHHTNNLIKDLLPDGSVTSLTNKIYANALSFKGAWKRPFEKYYTRDNDFYL 194 >At1g47710.1 68414.m05302 serpin, putative / serine protease inhibitor, putative similar to phloem serpin-1 [Cucurbita maxima] GI:9937311; contains Pfam profile PF00079: Serpin (serine protease inhibitor) Length = 391 Score = 51.6 bits (118), Expect = 5e-07 Identities = 39/180 (21%), Positives = 80/180 (44%), Gaps = 9/180 (5%) Frame = +3 Query: 111 DKTSLQFLKETYTS-SKDKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFL---GNGDYS 278 ++ S+ K T+ S++ NV+ SP + +++ + AG+ ++ +I FL + Sbjct: 11 NQVSMNLAKHVITTVSQNSNVIFSPASINVVLSIIAAGSAGATKDQILSFLKFSSTDQLN 70 Query: 279 GVANPYISLSKTFSEMNPD-YFTMANKIYVGNKYTLDEKFTITVRQ-YQSEVETIDFSDT 452 ++ +S N ++AN ++ + F + Y++ DF Sbjct: 71 SFSSEIVSAVLADGSANGGPKLSVANGAWIDKSLSFKPSFKQLLEDSYKAASNQADFQS- 129 Query: 453 KKAADII---NQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKE 623 KA ++I N WA ++T G I + + + D N ++F+G W+ F+ S T+E Sbjct: 130 -KAVEVIAEVNSWAEKETNGLITEVLPEGSADSMTKLIFANALYFKGTWNEKFDESLTQE 188 >At2g35580.1 68415.m04357 serpin family protein / serine protease inhibitor family protein similar to protein zx [Hordeum vulgare subsp. vulgare] GI:19071, serpin [Triticum aestivum] GI:1885350; contains Pfam profile PF00079: Serpin (serine protease inhibitor) Length = 374 Score = 39.5 bits (88), Expect = 0.002 Identities = 24/99 (24%), Positives = 46/99 (46%), Gaps = 5/99 (5%) Frame = +3 Query: 348 ANKIYVGNKYTLDEKFT-ITVRQYQSEVETIDFSDTKKAADI---INQWANEKTRGHIKS 515 AN +++ ++ F + + Y++ +DF KA ++ +N W ++T G I + Sbjct: 95 ANGLWIEKTLNVEPSFKDLLLNSYKAAFNRVDFRT--KADEVNREVNSWVEKQTNGLITN 152 Query: 516 PISDDTIDPNAAAALF-NVIFFQGHWHVPFNASETKEKD 629 + + +F N +FF G W F+ S TK+ D Sbjct: 153 LLPSNPKSAPLTDHIFANALFFNGRWDSQFDPSLTKDSD 191 >At1g63280.1 68414.m07154 serpin-related / serine protease inhibitor-related similar to protein zx [Hordeum vulgare subsp. vulgare] GI:19071, serpin [Triticum aestivum] GI:1885346 Length = 120 Score = 36.3 bits (80), Expect = 0.019 Identities = 14/53 (26%), Positives = 27/53 (50%) Frame = +3 Query: 471 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEKD 629 +N+WA++ T G I + ++ N ++F+G W F+ S TK+ + Sbjct: 16 LNKWASDHTNGLIIDLLPRGSVKSETVQVYGNALYFKGAWENKFDKSSTKDNE 68 >At1g62170.1 68414.m07013 serpin family protein / serine protease inhibitor family protein similar to phloem serpin-1 GI:9937311 from [Cucurbita maxima]; contains Pfam profile PF00079: Serpin (serine protease inhibitor) Length = 433 Score = 33.1 bits (72), Expect = 0.17 Identities = 17/65 (26%), Positives = 31/65 (47%), Gaps = 1/65 (1%) Frame = +3 Query: 435 IDF-SDTKKAADIINQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNAS 611 +DF S ++ +N WA+ T G IK + ++ + ++F+G W ++ S Sbjct: 190 VDFRSKAEEVRTEVNAWASSHTNGLIKDLLPRGSVTSLTDRVYGSALYFKGTWEEKYSKS 249 Query: 612 ETKEK 626 TK K Sbjct: 250 MTKCK 254 >At1g51330.1 68414.m05772 serpin-related / serine protease inhibitor-related similar to serpin [Hordeum vulgare subsp. vulgare] CAA64599.1 GI:1197577 Length = 193 Score = 32.7 bits (71), Expect = 0.23 Identities = 15/52 (28%), Positives = 23/52 (44%) Frame = +3 Query: 471 INQWANEKTRGHIKSPISDDTIDPNAAAALFNVIFFQGHWHVPFNASETKEK 626 +N WA T G IK+ + ++ N ++F+G W F S T K Sbjct: 39 VNSWALRHTNGLIKNLLPPGSVTNQTIKIYGNALYFKGAWENKFGKSMTIHK 90 >At1g05960.1 68414.m00625 expressed protein similar to hypothetical protein GB:AAF80120 GI:8810459 from [Arabidopsis thaliana ] Length = 982 Score = 29.5 bits (63), Expect = 2.2 Identities = 12/41 (29%), Positives = 23/41 (56%) Frame = -2 Query: 655 HDGCRVNMESFSLVSLALKGTCQ*PWKNITLKSAAAAFGSM 533 H C +E+ ++ S+ L GT + PW + +++ A GS+ Sbjct: 486 HADCTTRVEAHNIFSVVLLGTLRLPWSDQHKETSEAVSGSL 526 >At3g02280.1 68416.m00209 flavodoxin family protein low similarity to SP|Q05001 NADPH-cytochrome P450 reductase (EC 1.6.2.4) {Catharanthus roseus}, similar to NADPH-dependent FMN and FAD containing oxidoreductase [Homo sapiens] GI:6694369; contains Pfam profiles PF00258: flavodoxin, PF00667: FAD binding domain, PF00175: Oxidoreductase NAD-binding domain Length = 623 Score = 28.7 bits (61), Expect = 3.8 Identities = 22/80 (27%), Positives = 40/80 (50%), Gaps = 7/80 (8%) Frame = +3 Query: 231 GSRAEIDKFLGNGD----YSGVANPY-ISLSKTFSEMNPDYFTMANKIYVGNKYTLDE-K 392 G+ I+K LG+ Y G +P+ +SL +T ++NP YF + + +D+ K Sbjct: 123 GATTIIEKGLGDDQHPSGYEGTLDPWMLSLWRTLYQINPKYFPKGPDVKIPQDEVIDKPK 182 Query: 393 FTITV-RQYQSEVETIDFSD 449 + I +Q + E + + SD Sbjct: 183 YRILFHKQEKLEPKLLSDSD 202 >At1g70900.1 68414.m08181 expressed protein Length = 244 Score = 28.7 bits (61), Expect = 3.8 Identities = 19/66 (28%), Positives = 32/66 (48%) Frame = +3 Query: 162 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 341 K +S +GV + LY + SR + K+L DY+ +A I L++ E NP + Sbjct: 103 KVYANSLIGVGIASSLYHS-----SRGKFRKYLRWADYTMIATATICLTRALREENPKFL 157 Query: 342 TMANKI 359 A+ + Sbjct: 158 MAASAL 163 >At1g23110.1 68414.m02889 hypothetical protein Length = 261 Score = 28.7 bits (61), Expect = 3.8 Identities = 20/66 (30%), Positives = 32/66 (48%) Frame = +3 Query: 162 KNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYF 341 K +S +GV + LY A SR ++ K+L DY+ +A I LS+ NP + Sbjct: 120 KVYANSLIGVGIASSLYHA-----SRGKLRKYLRWVDYTMIATTTICLSRALRNENPKFL 174 Query: 342 TMANKI 359 A+ + Sbjct: 175 MAASAL 180 >At5g19010.1 68418.m02258 mitogen-activated protein kinase, putative / MAPK, putative (MPK16) mitogen-activated protein kinase (MAPK)(AtMPK16), PMID:12119167; similar to ATMPK9, Arabidopsis thaliana, EMBL:AB038694 Length = 567 Score = 27.9 bits (59), Expect = 6.6 Identities = 11/24 (45%), Positives = 15/24 (62%) Frame = +3 Query: 225 GEGSRAEIDKFLGNGDYSGVANPY 296 GEGSR I++ +G G Y V + Y Sbjct: 20 GEGSRYRIEEVIGKGSYGVVCSAY 43 >At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein similar to alpha-mannosidase II SP:P27046 from [Mus musculus] Length = 1173 Score = 27.9 bits (59), Expect = 6.6 Identities = 10/29 (34%), Positives = 18/29 (62%) Frame = -2 Query: 214 LYRSISIITPNGEDTTFLSFDDVYVSLRN 128 LYR+ +++ P G+D ++S D+ RN Sbjct: 401 LYRTNTLLIPLGDDFRYISIDEAEAQFRN 429 >At4g01290.1 68417.m00170 expressed protein Length = 991 Score = 27.9 bits (59), Expect = 6.6 Identities = 18/54 (33%), Positives = 26/54 (48%), Gaps = 4/54 (7%) Frame = +1 Query: 349 PIKSTSEINILWMRNSRSLSVSTKAKLKLLT----SAIPKKQLTSLISGLMRRP 498 P+ + + + M R SV +A LL+ S+ PK Q T L+S RRP Sbjct: 584 PVDQDTSVPSVKMTKQRKTSVDDQASQHLLSLLQRSSDPKSQDTQLLSATERRP 637 >At3g25140.1 68416.m03139 glycosyl transferase family 8 protein contains Pfam profile: PF01501 glycosyl transferase family 8 Length = 559 Score = 27.9 bits (59), Expect = 6.6 Identities = 17/77 (22%), Positives = 35/77 (45%) Frame = +3 Query: 165 NVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKTFSEMNPDYFT 344 +VV+ + + + +++K +G+ E+ DY+ + + Y+ + K N F Sbjct: 281 HVVTDKMNLGAMQVMFKLKEYKGAHVEVKAV---EDYTFLNSSYVPVLKQLESANLQKFY 337 Query: 345 MANKIYVGNKYTLDEKF 395 NK+ K T + KF Sbjct: 338 FENKLENATKDTTNMKF 354 >At5g35940.1 68418.m04325 jacalin lectin family protein similar to myrosinase-binding protein homolog [Arabidopsis thaliana] GI:2997767; contains Pfam profile PF01419 jacalin-like lectin domain Length = 444 Score = 27.5 bits (58), Expect = 8.7 Identities = 18/61 (29%), Positives = 26/61 (42%), Gaps = 1/61 (1%) Frame = +3 Query: 159 DKNVVSSPLGVMMLMLLYKAGAGEGSRAEIDKFLGNGDYSGVANPYISLSKT-FSEMNPD 335 DKNV S LG + +L +G + + GDY G+ Y++ T M D Sbjct: 131 DKNVYS--LGAYFIKVLATKSGMQGGQNTGKSYDYGGDYDGIRKVYVTYDGTSIRHMRVD 188 Query: 336 Y 338 Y Sbjct: 189 Y 189 >At4g30550.1 68417.m04336 glutamine amidotransferase class-I domain-containing protein similar to defense-related protein [Brassica carinata] GI:14009290, component of aniline dioxygenase (GMP synthase like protein) - Acinetobacter sp.,PID:d1013698; contains Pfam profile PF00117: glutamine amidotransferase class-I Length = 249 Score = 27.5 bits (58), Expect = 8.7 Identities = 19/60 (31%), Positives = 27/60 (45%), Gaps = 8/60 (13%) Frame = +3 Query: 24 LLAVIAVCNVRAFYSSDH--------EYNRTALGDAIDKTSLQFLKETYTSSKDKNVVSS 179 LLA VCNV F DH EYN+ L + +D+ L E + K K+ + + Sbjct: 172 LLASSEVCNVEMFSIGDHFFCIQGHPEYNKEILFEIVDRVLNMKLMEQEFADKAKSTMET 231 >At3g55340.1 68416.m06146 RNA recognition motif (RRM)-containing protein low similarity to nucleolar phosphoprotein (Nopp52), Tetrahymena thermophila, EMBL:TT51555; contains InterPro entry IPR000504: RNA-binding region RNP-1 (RNA recognition motif) (RRM) Length = 597 Score = 27.5 bits (58), Expect = 8.7 Identities = 13/41 (31%), Positives = 19/41 (46%) Frame = +3 Query: 249 DKFLGNGDYSGVANPYISLSKTFSEMNPDYFTMANKIYVGN 371 D++L Y P I KT S P+ N++Y+GN Sbjct: 228 DRYLTIQQYVKTTTPSIPRRKTSSGFAPEMVDGYNRVYIGN 268 >At2g20170.1 68415.m02358 hypothetical protein and grail contains Pfam profile PF03080: Arabidopsis proteins of unknown function Length = 401 Score = 27.5 bits (58), Expect = 8.7 Identities = 13/30 (43%), Positives = 19/30 (63%) Frame = +3 Query: 87 RTALGDAIDKTSLQFLKETYTSSKDKNVVS 176 RT L D I L++L YT+SKDK+ ++ Sbjct: 121 RTTLEDLIQIQRLKYLGVKYTTSKDKDFLN 150 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 14,772,530 Number of Sequences: 28952 Number of extensions: 311638 Number of successful extensions: 972 Number of sequences better than 10.0: 21 Number of HSP's better than 10.0 without gapping: 942 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 970 length of database: 12,070,560 effective HSP length: 79 effective length of database: 9,783,352 effective search space used: 1438152744 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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