Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_N23
(459 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to SP|Q... 122 2e-28
At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to calr... 120 6e-28
At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to simi... 104 3e-23
At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to simi... 104 3e-23
At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin... 44 4e-05
At5g07340.1 68418.m00838 calnexin, putative identical to calnexi... 39 0.001
At5g08430.1 68418.m00993 SWIB complex BAF60b domain-containing p... 29 1.5
At1g74190.1 68414.m08592 leucine-rich repeat family protein cont... 29 1.5
At1g74170.1 68414.m08590 leucine-rich repeat family protein cont... 29 1.5
At1g58210.1 68414.m06610 kinase interacting family protein simil... 28 2.6
At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase fam... 28 2.6
At5g33380.1 68418.m03963 hypothetical protein 27 4.6
At5g04710.1 68418.m00480 aspartyl aminopeptidase, putative simil... 27 6.1
At2g43440.1 68415.m05399 F-box family protein contains Pfam PF00... 27 6.1
At2g07280.1 68415.m00835 hypothetical protein 27 8.1
At1g75420.1 68414.m08761 glycosyl transferase family 1 protein c... 27 8.1
>At1g09210.1 68414.m01028 calreticulin 2 (CRT2) identical to
SP|Q38858 Calreticulin 2 precursor {Arabidopsis
thaliana}
Length = 424
Score = 122 bits (293), Expect = 2e-28
Identities = 61/109 (55%), Positives = 76/109 (69%), Gaps = 2/109 (1%)
Frame = +3
Query: 96 VFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYALS 269
V FEE+F DD WE+ WV SE + G++K TAG + DA DKG+QTSED RFYA+S
Sbjct: 24 VIFEERF-DDGWENRWVKSEWKKDDNTAGEWKHTAGNWSGDAN-DKGIQTSEDYRFYAIS 81
Query: 270 RKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETP 416
+F FSN+ K LV QFSVKHEQ +DCGGGY+K+ +DQK G+TP
Sbjct: 82 AEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTP 130
Score = 31.1 bits (67), Expect = 0.37
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +1
Query: 415 PYEIMFGPDICG 450
PY IMFGPDICG
Sbjct: 130 PYSIMFGPDICG 141
>At1g56340.1 68414.m06476 calreticulin 1 (CRT1) identical to
calreticulin (crt1) GI:2052379 [Arabidopsis thaliana]
Length = 425
Score = 120 bits (288), Expect = 6e-28
Identities = 60/112 (53%), Positives = 76/112 (67%), Gaps = 2/112 (1%)
Frame = +3
Query: 87 NCEVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFY 260
+ EV FEEKF +D WE WV S+ + G++K TAG + DA DKG+QTSED RFY
Sbjct: 21 SAEVIFEEKF-EDGWEKRWVKSDWKKDDNTAGEWKHTAGNWSGDAN-DKGIQTSEDYRFY 78
Query: 261 ALSRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETP 416
A+S +F FSN+ K LV QFSVKHEQ +DCGGGY+K+ +DQ G+TP
Sbjct: 79 AISAEFPEFSNKDKTLVFQFSVKHEQKLDCGGGYMKLLSDDVDQTKFGGDTP 130
Score = 31.1 bits (67), Expect = 0.37
Identities = 11/12 (91%), Positives = 11/12 (91%)
Frame = +1
Query: 415 PYEIMFGPDICG 450
PY IMFGPDICG
Sbjct: 130 PYSIMFGPDICG 141
>At1g08450.2 68414.m00935 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 370
Score = 104 bits (250), Expect = 3e-23
Identities = 53/110 (48%), Positives = 72/110 (65%), Gaps = 2/110 (1%)
Frame = +3
Query: 93 EVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYAL 266
E+F EE F + W+S WV S+ E G FK TAGK+ D + +KG+QT DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDPD-NKGIQTYNDAKHYAI 86
Query: 267 SRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETP 416
S K FSN+ + LVVQ+SVK EQDI+CGG Y+K+ ++QK G+TP
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTP 136
>At1g08450.1 68414.m00934 calreticulin 3 (CRT3) identical to similar
to SP|O04153 Calreticulin 3 precursor {Arabidopsis
thaliana}
Length = 424
Score = 104 bits (250), Expect = 3e-23
Identities = 53/110 (48%), Positives = 72/110 (65%), Gaps = 2/110 (1%)
Frame = +3
Query: 93 EVFFEEKFSDDSWESNWVYSEHPGKE--FGKFKLTAGKFYNDAEADKGLQTSEDARFYAL 266
E+F EE F + W+S WV S+ E G FK TAGK+ D + +KG+QT DA+ YA+
Sbjct: 29 EIFLEEHF-EGGWKSRWVLSDWKRNEGKAGTFKHTAGKWPGDPD-NKGIQTYNDAKHYAI 86
Query: 267 SRKFKPFSNEGKPLVVQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETP 416
S K FSN+ + LVVQ+SVK EQDI+CGG Y+K+ ++QK G+TP
Sbjct: 87 SAKIPEFSNKNRTLVVQYSVKIEQDIECGGAYIKLLSGYVNQKQFGGDTP 136
Score = 30.3 bits (65), Expect = 0.65
Identities = 10/12 (83%), Positives = 11/12 (91%)
Frame = +1
Query: 415 PYEIMFGPDICG 450
PY +MFGPDICG
Sbjct: 136 PYSLMFGPDICG 147
>At5g61790.1 68418.m07754 calnexin 1 (CNX1) identical to calnexin
homolog 1, Arabidopsis thaliana, EMBL:AT08315
[SP|P29402]
Length = 530
Score = 44.4 bits (100), Expect = 4e-05
Identities = 24/84 (28%), Positives = 47/84 (55%), Gaps = 1/84 (1%)
Frame = +3
Query: 120 DDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYALSRKF-KPFSNE 296
D+ ++ W+ S++ E G +K ++ D GL SE AR Y + ++ +P + +
Sbjct: 36 DEPFDGRWIVSKNSDYE-GVWKHAK----SEGHEDYGLLVSEKARKYGIVKELDEPLNLK 90
Query: 297 GKPLVVQFSVKHEQDIDCGGGYLK 368
+V+Q+ V+ ++ ++CGG YLK
Sbjct: 91 EGTVVLQYEVRFQEGLECGGAYLK 114
Score = 31.5 bits (68), Expect = 0.28
Identities = 12/22 (54%), Positives = 14/22 (63%)
Frame = +1
Query: 385 WTRRTCMARHPYEIMFGPDICG 450
WT + + PY IMFGPD CG
Sbjct: 123 WTPQGFDSESPYSIMFGPDKCG 144
>At5g07340.1 68418.m00838 calnexin, putative identical to calnexin
homolog 2 from Arabidopsis thaliana [SP|Q38798], strong
similarity to calnexin homolog 1, Arabidopsis thaliana,
EMBL:AT08315 [SP|P29402]; contains Pfam profile PF00262
calreticulin family
Length = 532
Score = 39.1 bits (87), Expect = 0.001
Identities = 26/87 (29%), Positives = 48/87 (55%), Gaps = 4/87 (4%)
Frame = +3
Query: 120 DDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAEADKGLQTSEDARFYALSRKF---KPFS 290
D+ ++ WV SE + G +K + ++D GL SE A+ Y + ++ +P +
Sbjct: 36 DEPFDGRWVVSEKAEYQ-GVWKHEKSEGHDDY----GLLVSEKAKKYGIVKELDVDEPLN 90
Query: 291 -NEGKPLVVQFSVKHEQDIDCGGGYLK 368
NEG +V+Q+ + ++ ++CGG YLK
Sbjct: 91 LNEGT-VVLQYEARFQEGLECGGAYLK 116
Score = 28.3 bits (60), Expect = 2.6
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = +1
Query: 415 PYEIMFGPDICG 450
PY IMFGPD CG
Sbjct: 135 PYSIMFGPDKCG 146
>At5g08430.1 68418.m00993 SWIB complex BAF60b domain-containing
protein / plus-3 domain-containing protein / GYF
domain-containing protein similar to CPRF interacting
protein [Petroselinum crispum] GI:9588690; contains Pfam
profiles PF02201: BAF60b domain of the SWIB complex,
PF03126: Plus-3 domain, PF02213: GYF domain; contains
non-conensus AT-AC splice sites at intron 5
Length = 553
Score = 29.1 bits (62), Expect = 1.5
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = +3
Query: 117 SDDSWESNWVYSEHPGKEFGKFKLTAGKFYNDAE 218
S D + NW+Y + G G F LT K ++DAE
Sbjct: 492 SYDKEKLNWLYKDPQGLVQGPFSLTQLKAWSDAE 525
>At1g74190.1 68414.m08592 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; contains similarity to Cf-2.1
[Lycopersicon pimpinellifolium] gi|1184075|gb|AAC15779
Length = 965
Score = 29.1 bits (62), Expect = 1.5
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +3
Query: 312 VQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPL 419
++F+ KH D GG +F L + ++ GE P+
Sbjct: 762 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPV 797
>At1g74170.1 68414.m08590 leucine-rich repeat family protein
contains leucine rich-repeat (LRR) domains Pfam:PF00560,
INTERPRO:IPR001611; similar to Cf-2.2 [Lycopersicon
pimpinellifolium] gi|1184077|gb|AAC15780
Length = 1068
Score = 29.1 bits (62), Expect = 1.5
Identities = 11/36 (30%), Positives = 19/36 (52%)
Frame = +3
Query: 312 VQFSVKHEQDIDCGGGYLKVFDCRLDQKDMHGETPL 419
++F+ KH D GG +F L + ++ GE P+
Sbjct: 830 IEFATKHRYDAYMGGNLKLLFGMDLSENELSGEIPV 865
>At1g58210.1 68414.m06610 kinase interacting family protein similar
to kinase interacting protein 1 (GI:13936326) {Petunia
integrifolia}
Length = 1246
Score = 28.3 bits (60), Expect = 2.6
Identities = 15/29 (51%), Positives = 19/29 (65%), Gaps = 1/29 (3%)
Frame = +3
Query: 210 DAEADKGLQTSEDARFYALSRKF-KPFSN 293
+AE +KG T+ RFYAL KF KP S+
Sbjct: 583 EAEIEKGRITTAKERFYALRNKFEKPESD 611
>At1g53500.1 68414.m06066 NAD-dependent epimerase/dehydratase family
protein low similarity to dTDP-D-glucose-4,6-dehydratase
from Aneurinibacillus thermoaerophilus GI:16357461,
Sphingomonas sp. GI:1314581; contains Pfam profile
PF01370: NAD dependent epimerase/dehydratase family;
putative NDP-rhamnose synthase (rhm2 gene) GI:31559258
Length = 667
Score = 28.3 bits (60), Expect = 2.6
Identities = 18/63 (28%), Positives = 24/63 (38%)
Frame = -3
Query: 202 NFPAVNLNLPNSFPGCSLYTQFDSHESSENFSSKNTSQFIDEIANKLAIASTADFIF*FT 23
N+P + + + CS D SS NF D++ N L I D I F
Sbjct: 31 NYPDYKIVVLDKLDYCSDLKNLDPSFSSPNFKFVKGDIASDDLVNYLLITENIDTIMHFA 90
Query: 22 AST 14
A T
Sbjct: 91 AQT 93
>At5g33380.1 68418.m03963 hypothetical protein
Length = 154
Score = 27.5 bits (58), Expect = 4.6
Identities = 15/43 (34%), Positives = 20/43 (46%)
Frame = -1
Query: 390 GPVYSRIPSGNRLRSRCPVRASH*TVPPKVCPHC*MA*ISSIG 262
G + +RI + R P R T+ P+V HC ISS G
Sbjct: 111 GDLRARIGFSVIVTHRVPTREKEQTISPRVSSHCARGSISSFG 153
>At5g04710.1 68418.m00480 aspartyl aminopeptidase, putative similar
to SP|Q9ULA0 Aspartyl aminopeptidase (EC 3.4.11.21)
{Homo sapiens}; contains Pfam profile PF02127:
Aminopeptidase I zinc metalloprotease (M18)
Length = 526
Score = 27.1 bits (57), Expect = 6.1
Identities = 15/33 (45%), Positives = 21/33 (63%)
Frame = -3
Query: 181 NLPNSFPGCSLYTQFDSHESSENFSSKNTSQFI 83
NL +SF C+L DS ESSEN S+++ + I
Sbjct: 311 NLASSF--CALRALIDSCESSENLSTEHDIRMI 341
>At2g43440.1 68415.m05399 F-box family protein contains Pfam
PF00646: F-box domain; contains TIGRFAM TIGR01640 :
F-box protein interaction domain
Length = 791
Score = 27.1 bits (57), Expect = 6.1
Identities = 12/37 (32%), Positives = 20/37 (54%)
Frame = -2
Query: 389 VQSTVEYLQVTASAVDVLFVLHTELYHQRFALIAEWL 279
V ++ +LQ++A + + LH E YH L A W+
Sbjct: 197 VNGSIYWLQISAGYIILAMDLHEESYHGVHHLPATWV 233
>At2g07280.1 68415.m00835 hypothetical protein
Length = 401
Score = 26.6 bits (56), Expect = 8.1
Identities = 9/21 (42%), Positives = 12/21 (57%)
Frame = +3
Query: 99 FFEEKFSDDSWESNWVYSEHP 161
F E F D+ E +W + EHP
Sbjct: 210 FLRELFGKDTGEQDWTHFEHP 230
>At1g75420.1 68414.m08761 glycosyl transferase family 1 protein
contains Pfam glycosyl transferase, group 1 family
protein domain PF00534
Length = 463
Score = 26.6 bits (56), Expect = 8.1
Identities = 12/39 (30%), Positives = 24/39 (61%)
Frame = -3
Query: 172 NSFPGCSLYTQFDSHESSENFSSKNTSQFIDEIANKLAI 56
+SF CS+ +QF ++ E+ ++K S +D + +KL +
Sbjct: 32 SSFETCSISSQFVEEKNGESSAAKFQSNPLDFMKSKLVL 70
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,695,686
Number of Sequences: 28952
Number of extensions: 217338
Number of successful extensions: 616
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 596
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 611
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 762235320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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