BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_N05
(581 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|c... 29 0.50
SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|ch... 29 0.65
SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|ch... 28 0.87
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 26 3.5
SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces... 26 4.6
SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces po... 25 6.1
SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme Mms2|Schizos... 25 8.1
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 25 8.1
>SPAC3H1.06c |||membrane transporter |Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 29.1 bits (62), Expect = 0.50
Identities = 15/38 (39%), Positives = 22/38 (57%)
Frame = -2
Query: 286 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVFL 173
CI GF ++ + +RTR + PS +LS T V+ FL
Sbjct: 325 CIAGFVVNEFYTTRTRIITPS-AFKTLSLTSVMVTSFL 361
>SPAC20H4.06c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 534
Score = 28.7 bits (61), Expect = 0.65
Identities = 14/30 (46%), Positives = 19/30 (63%), Gaps = 3/30 (10%)
Frame = -3
Query: 456 RGSAVRKSVQCS---PPLWGYVQVYNSQMP 376
+G ++ KS CS PPL G+V V+N Q P
Sbjct: 256 KGKSIIKSQNCSDGLPPLPGFVVVFNLQSP 285
>SPAC1399.02 |||membrane transporter|Schizosaccharomyces pombe|chr
1|||Manual
Length = 589
Score = 28.3 bits (60), Expect = 0.87
Identities = 14/38 (36%), Positives = 22/38 (57%)
Frame = -2
Query: 286 CILGFTLSACFISRTRAVKPSLCSDSLSSTGVVPNVFL 173
CI GF ++ + +RTR + PS +LS + V+ FL
Sbjct: 325 CIAGFVVNELYTTRTRIIAPS-AFQTLSLSSVMVTSFL 361
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.2 bits (55), Expect = 3.5
Identities = 21/68 (30%), Positives = 30/68 (44%), Gaps = 5/68 (7%)
Frame = +3
Query: 204 DKESEHREG---FTALVRE--MKQALNVKPNMQLVISVLPNVNSSIYFDVPSIINLVDIV 368
D ES H EG F + E MK+ALN ++ +S ++ I FD I D V
Sbjct: 985 DYESHHGEGNVHFNVTLTEAGMKEALNESLEVKFKLSRTQATSNMIAFDASGRIKKYDSV 1044
Query: 369 NIQAFDYY 392
++Y
Sbjct: 1045 EDILTEFY 1052
>SPBC3H7.03c |||2-oxoglutarate dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1009
Score = 25.8 bits (54), Expect = 4.6
Identities = 10/22 (45%), Positives = 12/22 (54%)
Frame = -1
Query: 368 DNVDQVYDAWDVKVNGAIYVWQ 303
D VD++YDAW N WQ
Sbjct: 54 DYVDEMYDAWKKDPNSVHASWQ 75
>SPBC16G5.12c |top3||DNA topoisomerase III|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 622
Score = 25.4 bits (53), Expect = 6.1
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = +3
Query: 33 QARTAFTNSALLLAEQYGFDGIDLSWQLPK 122
Q T F S+L +A G+D I L W L K
Sbjct: 531 QGVTEFVPSSLGVALAKGYDEIGLEWSLTK 560
>SPCC338.05c |mms2|spm2|ubiquitin conjugating enzyme
Mms2|Schizosaccharomyces pombe|chr 3|||Manual
Length = 139
Score = 25.0 bits (52), Expect = 8.1
Identities = 11/32 (34%), Positives = 16/32 (50%)
Frame = +3
Query: 327 YFDVPSIINLVDIVNIQAFDYYTPERNPKEAD 422
Y D P I+ V +N+ D T + NP + D
Sbjct: 68 YPDAPPIVTFVSRINLPGVDGETGKVNPHKID 99
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.0 bits (52), Expect = 8.1
Identities = 13/49 (26%), Positives = 22/49 (44%)
Frame = +3
Query: 432 PIYAPQNRDPLQNADAAINYWIQNGAPTHKLVLGISTTGRTWKLDSDSE 578
P PQ+ + +QN + + + G P K V+ + T SDS+
Sbjct: 777 PESPPQSYESIQNDNELLQELLSMGFPREKAVIALEATNYDVNEVSDSD 825
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,275,500
Number of Sequences: 5004
Number of extensions: 45954
Number of successful extensions: 158
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 153
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 158
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 250133048
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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