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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0001_M23
         (576 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATP...    99   2e-21
At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putati...    97   7e-21
At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putati...    83   1e-16
At5g05400.1 68418.m00582 disease resistance protein (CC-NBS-LRR ...    28   3.9  
At5g51530.1 68418.m06390 ubiquitin carboxyl-terminal hydrolase-r...    27   9.0  

>At4g11150.1 68417.m01807 vacuolar ATP synthase subunit E / V-ATPase
           E subunit / vacuolar proton pump E subunit (VATE)
           identical to SP|Q39258 Vacuolar ATP synthase subunit E
           (EC 3.6.3.14) (V-ATPase E subunit) (Vacuolar proton pump
           E subunit) {Arabidopsis thaliana}
          Length = 230

 Score = 98.7 bits (235), Expect = 2e-21
 Identities = 56/156 (35%), Positives = 81/156 (51%)
 Frame = +1

Query: 106 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMXXXXXXXX 285
           ++D DV +QI+ M+ FI Q                FNIEK +LV+ ++ KI         
Sbjct: 1   MNDGDVSRQIQQMVRFIRQEAEEKANEISVSAEEEFNIEKLQLVEAEKKKIRQDYEKKEK 60

Query: 286 XXXXXXXIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSDLLTTLIVQ 465
                  I  S  LN +R+KVL+ ++D V  + D+A K L  V +D   Y  LL  LIVQ
Sbjct: 61  QADVRKKIDYSMQLNASRIKVLQAQDDIVNAMKDQAAKDLLNVSRDEYAYKQLLKDLIVQ 120

Query: 466 ALFQLMEPSVTIRVRQVDKSQVESILARAQQDYKTK 573
            L +L EPSV +R R+ D   VE++L  A+++Y  K
Sbjct: 121 CLLRLKEPSVLLRCREEDLGLVEAVLDDAKEEYAGK 156


>At3g08560.1 68416.m00993 vacuolar ATP synthase subunit E, putative
           / V-ATPase E subunit, putative / vacuolar proton pump E
           subunit, putative similar to SP|Q39258 Vacuolar ATP
           synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
           (Vacuolar proton pump E subunit) {Arabidopsis thaliana};
           contains Pfam profile PF01991: ATP synthase (E/31 kDa)
           subunit
          Length = 235

 Score = 97.1 bits (231), Expect = 7e-21
 Identities = 53/156 (33%), Positives = 81/156 (51%)
 Frame = +1

Query: 106 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMXXXXXXXX 285
           ++DADV KQI+ M+ FI Q                FNIE+ +L++  + K+         
Sbjct: 1   MNDADVSKQIQQMVRFIRQEAEEKANEISISAEEEFNIERLQLLESAKRKLRQDYDRKLK 60

Query: 286 XXXXXXXIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSDLLTTLIVQ 465
                  I  S  LN +R+K L+ ++D V  + D A K L  V  D   Y  LL +LI++
Sbjct: 61  QVDIRKRIDYSTQLNASRIKYLQAQDDVVTAMKDSAAKDLLRVSNDKNNYKKLLKSLIIE 120

Query: 466 ALFQLMEPSVTIRVRQVDKSQVESILARAQQDYKTK 573
           +L +L EPSV +R R++DK  VES++  A++ Y  K
Sbjct: 121 SLLRLKEPSVLLRCREMDKKVVESVIEDAKRQYAEK 156


>At1g64200.1 68414.m07273 vacuolar ATP synthase subunit E, putative
           / V-ATPase E subunit, putative / vacuolar proton pump E
           subunit, putative similar to SP|Q39258 Vacuolar ATP
           synthase subunit E (EC 3.6.3.14) (V-ATPase E subunit)
           (Vacuolar proton pump E subunit) {Arabidopsis thaliana};
           contains Pfam profile PF01991: ATP synthase (E/31 kDa)
           subunit
          Length = 237

 Score = 83.0 bits (196), Expect = 1e-16
 Identities = 55/162 (33%), Positives = 80/162 (49%), Gaps = 6/162 (3%)
 Frame = +1

Query: 106 LSDADVQKQIKHMMAFIEQXXXXXXXXXXXXXXXXFNIEKGRLVQQQRLKIMXXXXXXXX 285
           ++DAD   QI+ M+ FI Q                FNIEK +LV+ ++ KI         
Sbjct: 1   MNDADASIQIQQMVRFIRQEAEEKANEISISSEEEFNIEKLQLVEAEKKKIRQEYEKKEK 60

Query: 286 XXXXXXXIQSSNMLNQARLKVLKVREDHVRNVLDEARKRLAEVPKDTKL------YSDLL 447
                  I  S  LN +R+KVL+ ++D V  + +EA K+L +V +          Y  LL
Sbjct: 61  QVDVRKKIDYSMQLNASRIKVLQAQDDIVNAMKEEAAKQLLKVSQHGFFNHHHHQYKHLL 120

Query: 448 TTLIVQALFQLMEPSVTIRVRQVDKSQVESILARAQQDYKTK 573
             LIVQ L +L EP+V +R R+ D   VES+L  A ++Y  K
Sbjct: 121 KDLIVQCLLRLKEPAVLLRCREEDLDIVESMLDDASEEYCKK 162


>At5g05400.1 68418.m00582 disease resistance protein (CC-NBS-LRR
           class), putative domain signature CC-NBS-LRR exists,
           suggestive of a disease resistance protein.
          Length = 874

 Score = 28.3 bits (60), Expect = 3.9
 Identities = 15/37 (40%), Positives = 23/37 (62%)
 Frame = +1

Query: 340 LKVLKVREDHVRNVLDEARKRLAEVPKDTKLYSDLLT 450
           LK +KV+ED   N+LDE ++ L+EV        D+L+
Sbjct: 54  LKRIKVQEDRGLNLLDEVQQWLSEVESRVCEAHDILS 90


>At5g51530.1 68418.m06390 ubiquitin carboxyl-terminal
           hydrolase-related contains Pfam profiles PF00443:
           Ubiquitin carboxyl-terminal hydrolase, PF04780: Protein
           of unknown function (DUF629), PF04781: Protein of
           unknown function (DUF627)
          Length = 1149

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 12/39 (30%), Positives = 22/39 (56%)
 Frame = -1

Query: 216 VELLLGLSIDFFSLFIGLLFDEGHHMLDLLLNICIAERH 100
           +++ L L +D F+    L+  + H +L  LLNI I + +
Sbjct: 825 LDMTLKLILDLFTFLFSLISSQKHVVLQALLNIKILKEY 863


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,622,589
Number of Sequences: 28952
Number of extensions: 189674
Number of successful extensions: 460
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 450
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 458
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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