Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_M07
(635 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g51850.1 68416.m05686 calcium-dependent protein kinase, putat... 33 0.21
At4g24450.1 68417.m03505 starch excess protein-related similar t... 31 0.85
At5g61810.1 68418.m07756 mitochondrial substrate carrier family ... 30 1.1
At5g51050.1 68418.m06328 mitochondrial substrate carrier family ... 30 1.1
At5g44460.1 68418.m05448 calcium-binding protein, putative simil... 30 1.5
At5g07320.1 68418.m00836 mitochondrial substrate carrier family ... 29 2.0
At1g03550.1 68414.m00336 secretory carrier membrane protein (SCA... 29 2.6
At2g34020.1 68415.m04165 calcium-binding EF hand family protein ... 29 3.4
At5g51410.2 68418.m06374 LUC7 N_terminus domain-containing prote... 28 4.5
At5g51410.1 68418.m06373 LUC7 N_terminus domain-containing prote... 28 4.5
At5g23580.1 68418.m02767 calcium-dependent protein kinase 9 (CDP... 28 4.5
At2g20840.1 68415.m02456 secretory carrier membrane protein (SCA... 28 4.5
At1g32050.1 68414.m03943 secretory carrier membrane protein (SCA... 28 4.5
At5g37420.1 68418.m04502 hypothetical protein contains Pfam PF04... 28 6.0
At4g20780.1 68417.m03017 calcium-binding protein, putative simil... 28 6.0
At3g20430.1 68416.m02587 expressed protein 28 6.0
At2g17290.1 68415.m01997 calcium-dependent protein kinase isofor... 28 6.0
At1g23880.1 68414.m03012 NHL repeat-containing protein contains ... 28 6.0
At5g05440.1 68418.m00586 expressed protein low similarity to cyt... 27 7.9
At2g31500.1 68415.m03848 calcium-dependent protein kinase, putat... 27 7.9
At1g04930.1 68414.m00490 hydroxyproline-rich glycoprotein family... 27 7.9
>At3g51850.1 68416.m05686 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Arabidopsis thaliana] gi|836942|gb|AAA67655;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 528
Score = 32.7 bits (71), Expect = 0.21
Identities = 14/43 (32%), Positives = 26/43 (60%)
Frame = +3
Query: 276 ESKRRLGIIVDKIDTDNDGFVTLVELKDWIRYTQKRYIDDDVE 404
E + ++ +K+DTDNDG V++ ELK +R + + +V+
Sbjct: 355 EEVEDIKVMFNKMDTDNDGIVSIEELKAGLRDFSTQLAESEVQ 397
>At4g24450.1 68417.m03505 starch excess protein-related similar to
SEX1 [Arabidopsis thaliana] GI:12044358
Length = 1284
Score = 30.7 bits (66), Expect = 0.85
Identities = 17/63 (26%), Positives = 28/63 (44%), Gaps = 1/63 (1%)
Frame = +3
Query: 270 PEESKRRLGIIVDKIDTDNDGFVTLVELKD-WIRYTQKRYIDDDVERHWKQHNPSNEPSI 446
P E + G + D +V ++EL+D +R + D R +QHN + I
Sbjct: 60 PSEHSSKQGALQTTFVKSGDAYVVILELRDPRVRAIEFVLKDGSHNRWLRQHNGNFRVEI 119
Query: 447 PWD 455
PW+
Sbjct: 120 PWN 122
>At5g61810.1 68418.m07756 mitochondrial substrate carrier family
protein similar to peroxisomal Ca-dependent solute
carrier, Oryctolagus cuniculus,GI:2352427; contains
INTERPRO:IPR001993 Mitochondrial substrate carrier
family, INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 478
Score = 30.3 bits (65), Expect = 1.1
Identities = 10/43 (23%), Positives = 23/43 (53%)
Frame = +3
Query: 291 LGIIVDKIDTDNDGFVTLVELKDWIRYTQKRYIDDDVERHWKQ 419
L ++ +D DN+G +T E +D++ +++ HW++
Sbjct: 140 LASFMEHVDKDNNGIITFEEWRDFLLLNPHEATIENIYHHWER 182
>At5g51050.1 68418.m06328 mitochondrial substrate carrier family
protein similar to peroxisomal Ca-dependent solute
carrier [Oryctolagus cuniculus] GI:2352427; contains
INTERPRO:IPR001993 Mitochondrial substrate carrier
family, INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 487
Score = 30.3 bits (65), Expect = 1.1
Identities = 12/49 (24%), Positives = 23/49 (46%)
Frame = +3
Query: 273 EESKRRLGIIVDKIDTDNDGFVTLVELKDWIRYTQKRYIDDDVERHWKQ 419
E L V+ +D DNDG + E +D++ +++ HW++
Sbjct: 138 EIKDEELARFVEHVDKDNDGIIMFEEWRDFLLLYPHEATIENIYHHWER 186
>At5g44460.1 68418.m05448 calcium-binding protein, putative similar
to SP|Q09011 Calcium-binding protein CAST {Solanum
tuberosum}; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 181
Score = 29.9 bits (64), Expect = 1.5
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +3
Query: 243 QAKTFDQLPPEESKRRLGIIVDKIDTDNDGFVTLVEL 353
Q+ +F P + RL + D D +NDGF+T+ EL
Sbjct: 13 QSSSFRLRSPSLNALRLHRVFDLFDKNNDGFITVEEL 49
>At5g07320.1 68418.m00836 mitochondrial substrate carrier family
protein similar to peroxisomal Ca-dependent solute
carrier [Oryctolagus cuniculus] GI:2352427
(mitochondrial carrier superfamily); contains
INTERPRO:IPR001993 Mitochondrial substrate carrier
family, INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 479
Score = 29.5 bits (63), Expect = 2.0
Identities = 12/49 (24%), Positives = 24/49 (48%)
Frame = +3
Query: 273 EESKRRLGIIVDKIDTDNDGFVTLVELKDWIRYTQKRYIDDDVERHWKQ 419
E L V+ +D DN+G +T E +D++ +++ HW++
Sbjct: 135 EIDDEELARFVEHVDKDNNGTITFEEWRDFLLLYPHEATLENIYHHWER 183
>At1g03550.1 68414.m00336 secretory carrier membrane protein (SCAMP)
family protein contains Pfam domain, PF04144: SCAMP
family
Length = 283
Score = 29.1 bits (62), Expect = 2.6
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -3
Query: 267 VIDQKFWPDLHPKMHHDRTAYCGAHSCSVQH 175
VI++K WP+ P +HHD H +Q+
Sbjct: 89 VIEEKNWPEFFPLIHHDIPNEIPIHLQKIQY 119
>At2g34020.1 68415.m04165 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 462
Score = 28.7 bits (61), Expect = 3.4
Identities = 13/27 (48%), Positives = 17/27 (62%)
Frame = +3
Query: 279 SKRRLGIIVDKIDTDNDGFVTLVELKD 359
SK L + DKID + DG + + ELKD
Sbjct: 245 SKESLKSLFDKIDRNKDGKIQISELKD 271
>At5g51410.2 68418.m06374 LUC7 N_terminus domain-containing protein
similar to cisplatin resistance-associated overexpressed
protein [Homo sapiens] GI:6899846; contains Pfam profile
PF03194: LUC7 N_terminus
Length = 334
Score = 28.3 bits (60), Expect = 4.5
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = +3
Query: 132 PSPEETKRMMDHLSDAEHYRNEHHNKQFDHDAFLGEDQAKTFDQ 263
P +E++ SD E YR+ N+ D G D K +D+
Sbjct: 257 PREKESESKRSGSSDRERYRDRDRNRDGDRHRDRGRDYRKPYDR 300
>At5g51410.1 68418.m06373 LUC7 N_terminus domain-containing protein
similar to cisplatin resistance-associated overexpressed
protein [Homo sapiens] GI:6899846; contains Pfam profile
PF03194: LUC7 N_terminus
Length = 334
Score = 28.3 bits (60), Expect = 4.5
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = +3
Query: 132 PSPEETKRMMDHLSDAEHYRNEHHNKQFDHDAFLGEDQAKTFDQ 263
P +E++ SD E YR+ N+ D G D K +D+
Sbjct: 257 PREKESESKRSGSSDRERYRDRDRNRDGDRHRDRGRDYRKPYDR 300
>At5g23580.1 68418.m02767 calcium-dependent protein kinase 9 (CDPK9)
identical to calcium-dependent protein kinase
[Arabidopsis thaliana] gi|836938|gb|AAA67653; contains
protein kinase domain, Pfam:PF00069; contains EF hand
domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 490
Score = 28.3 bits (60), Expect = 4.5
Identities = 14/44 (31%), Positives = 22/44 (50%)
Frame = +3
Query: 273 EESKRRLGIIVDKIDTDNDGFVTLVELKDWIRYTQKRYIDDDVE 404
EE L + IDTD G +T ELKD +R ++ +++
Sbjct: 322 EEEIGGLKELFKMIDTDKSGTITFEELKDSMRRVGSELMESEIQ 365
>At2g20840.1 68415.m02456 secretory carrier membrane protein (SCAMP)
family protein contains Pfam domain, PF04144: SCAMP
family
Length = 282
Score = 28.3 bits (60), Expect = 4.5
Identities = 11/31 (35%), Positives = 17/31 (54%)
Frame = -3
Query: 267 VIDQKFWPDLHPKMHHDRTAYCGAHSCSVQH 175
VI++K WP P +HHD + H +Q+
Sbjct: 88 VIEEKNWPPFFPLIHHDISNEIPIHLQRIQY 118
>At1g32050.1 68414.m03943 secretory carrier membrane protein (SCAMP)
family protein contains Pfam domain, PF04144: SCAMP
family
Length = 264
Score = 28.3 bits (60), Expect = 4.5
Identities = 11/30 (36%), Positives = 15/30 (50%)
Frame = -3
Query: 264 IDQKFWPDLHPKMHHDRTAYCGAHSCSVQH 175
ID K WP P +HHD H+ +Q+
Sbjct: 89 IDDKNWPPFFPIIHHDIAKEIPVHAQKLQY 118
>At5g37420.1 68418.m04502 hypothetical protein contains Pfam PF04510
: Family of unknown function (DUF577)); common family
comprised of At5g37410, At5g37400, At5g37920,
At5g37460, At5g37650, At5g37470, At5g37420, At5g37430
Length = 579
Score = 27.9 bits (59), Expect = 6.0
Identities = 12/31 (38%), Positives = 20/31 (64%)
Frame = -3
Query: 210 AYCGAHSCSVQHQINDPSFVSSLQDLVLRRI 118
A+ GA C+V H I+DP + SL+++ + I
Sbjct: 425 AFTGAF-CAVIHLIDDPDYAESLKEIAYKMI 454
>At4g20780.1 68417.m03017 calcium-binding protein, putative similar
to SP|Q09011 Calcium-binding protein CAST {Solanum
tuberosum}; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 191
Score = 27.9 bits (59), Expect = 6.0
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = +3
Query: 243 QAKTFDQLPPEESKRRLGIIVDKIDTDNDGFVTLVEL 353
Q+ +F P + RL I D D + DGF+T+ EL
Sbjct: 14 QSSSFRLRSPSLNALRLQRIFDLFDKNGDGFITVEEL 50
>At3g20430.1 68416.m02587 expressed protein
Length = 265
Score = 27.9 bits (59), Expect = 6.0
Identities = 24/84 (28%), Positives = 41/84 (48%), Gaps = 6/84 (7%)
Frame = +3
Query: 141 EETKRMMDHLSDAEHYRNEHHNKQFDHDAFLGEDQA--KTFDQLPPEESKRRLGIIVDK- 311
EE + ++DH D+ +N+ + D +A LGE +T ++ K+R G ++DK
Sbjct: 29 EEGEIVVDHDLDSGERQNDDGDGVKDKEAILGEKNGLQQTNKNKRKKKKKKRKGPVMDKP 88
Query: 312 IDTD---NDGFVTLVELKDWIRYT 374
+ D D L E K ++ YT
Sbjct: 89 MSVDWFVRDTCRRLKEKKSYMIYT 112
>At2g17290.1 68415.m01997 calcium-dependent protein kinase isoform 6
(CPK6) identical to calmodulin-domain protein kinase
CDPK isoform 6 [Arabidopsis thaliana]
gi|1399275|gb|AAB03246; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 544
Score = 27.9 bits (59), Expect = 6.0
Identities = 18/54 (33%), Positives = 25/54 (46%)
Frame = +3
Query: 207 KQFDHDAFLGEDQAKTFDQLPPEESKRRLGIIVDKIDTDNDGFVTLVELKDWIR 368
KQF L + K + EE L + + +DTDN G +T ELK +R
Sbjct: 363 KQFSAMNKLKKMALKVIAESLSEEEIAGLRAMFEAMDTDNSGAITFDELKAGLR 416
>At1g23880.1 68414.m03012 NHL repeat-containing protein contains
Pfam profile PF01436: NHL repeat
Length = 545
Score = 27.9 bits (59), Expect = 6.0
Identities = 22/112 (19%), Positives = 49/112 (43%), Gaps = 2/112 (1%)
Frame = +3
Query: 258 DQLPPEESKRRL-GIIVDKIDTD-NDGFVTLVELKDWIRYTQKRYIDDDVERHWKQHNPS 431
D+ PP ES+ G I + D + L + + R ++ + + +Q++
Sbjct: 433 DEPPPVESRNATPGKIYPFMSKDATEKMQQLRQSRALYRSLDAEFLQEQQQEKHQQYHHR 492
Query: 432 NEPSIPWDVS*FYHTENIGRVTGVVFNKTTD*EDMRATFSPYKRAHIFIPYP 587
+ +IP+ + + ++ + +VF + + M + + RAH F+ YP
Sbjct: 493 HHSTIPYTL----YEQSSEKTNEIVFGPGQEQDQMNTHQNIHHRAHQFVSYP 540
>At5g05440.1 68418.m00586 expressed protein low similarity to
cytokinin-specific binding protein [Vigna radiata]
GI:4190976
Length = 203
Score = 27.5 bits (58), Expect = 7.9
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = +1
Query: 526 KRICERRFLHTNEHIFSYHIH 588
KR+C R +H EH+ +H H
Sbjct: 31 KRVCLTRGMHVPEHVAMHHTH 51
>At2g31500.1 68415.m03848 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase [Arabidopsis thaliana] gi|836942|gb|AAA67655;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 582
Score = 27.5 bits (58), Expect = 7.9
Identities = 16/49 (32%), Positives = 26/49 (53%)
Frame = +3
Query: 258 DQLPPEESKRRLGIIVDKIDTDNDGFVTLVELKDWIRYTQKRYIDDDVE 404
D LP EE + + +DTD +G +T EL+D ++ + D DV+
Sbjct: 362 DNLPNEEIAAIVQMF-QTMDTDKNGHLTFEELRDGLKKIGQVVPDGDVK 409
>At1g04930.1 68414.m00490 hydroxyproline-rich glycoprotein family
protein Common family member: At2g32840 [Arabidopsis
thaliana]
Length = 332
Score = 27.5 bits (58), Expect = 7.9
Identities = 17/45 (37%), Positives = 21/45 (46%), Gaps = 3/45 (6%)
Frame = -3
Query: 276 LPEVIDQKFWPDLHPKMHHDRTAYCG---AHSCSVQHQINDPSFV 151
LP + P HP ++AY AHS V+ QI DPS V
Sbjct: 46 LPPPTYRPIAPLRHPNPFQQQSAYSNNLYAHSIPVRRQIQDPSAV 90
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 13,800,480
Number of Sequences: 28952
Number of extensions: 285741
Number of successful extensions: 984
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 923
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 978
length of database: 12,070,560
effective HSP length: 78
effective length of database: 9,812,304
effective search space used: 1305036432
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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