Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_M03
(552 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 94 1e-20
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 79 3e-16
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 74 2e-14
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 59 4e-10
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 53 3e-08
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 31 0.11
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 25 5.6
SPAC12G12.16c ||SPAC18B11.01c|nuclease, XP-G family|Schizosaccha... 25 7.4
SPCC1672.09 |||triglyceride lipase-cholesterol esterase |Schizos... 25 7.4
SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces ... 25 9.8
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 93.9 bits (223), Expect = 1e-20
Identities = 50/137 (36%), Positives = 71/137 (51%)
Frame = +3
Query: 21 LFINNEWVDAVSKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLLDA 200
LFINN+ VD+V +P E +I +VA+ F + WR +
Sbjct: 25 LFINNQHVDSVHGGRVKVYSPSTEKLICEVADADEEDVDIAVKVARAAFQTDAPWRKFSS 84
Query: 201 SQRGLLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADKILGN 380
+QRG L +LA +E++ +YLA +ETLDNGK + A +V A+ RYY G ADK G
Sbjct: 85 AQRGRCLSRLADCIEQNLEYLASIETLDNGKSITLARGDVQAAADCFRYYGGWADKDYGQ 144
Query: 381 TIPADGEVLTFTLKEPV 431
TI D + +T EP+
Sbjct: 145 TIETDIKRFAYTRHEPI 161
Score = 60.5 bits (140), Expect = 2e-10
Identities = 24/39 (61%), Positives = 30/39 (76%)
Frame = +2
Query: 434 VCGQILPWNYPIPMFIWNIAPALAAGCTVVVKPAEQTPL 550
VCGQI+PWN+P M W IAPA+A G T+++K AE TPL
Sbjct: 163 VCGQIIPWNFPFLMCAWKIAPAVACGNTIILKTAELTPL 201
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 79.4 bits (187), Expect = 3e-16
Identities = 49/138 (35%), Positives = 69/138 (50%), Gaps = 1/138 (0%)
Frame = +3
Query: 21 LFINNEWVDAVSKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLLDA 200
L+IN EW S +T+ T++P E VI +V F W+ +
Sbjct: 23 LYINGEWHK--SAETWETVDPSIEEVIAKVYLAGEKEIDYAVKSAKEAF---KTWKKVPG 77
Query: 201 SQRGLLLFKLATLMERDAKYLAELETLDNGKP-VKQAEQEVLWASGIVRYYAGKADKILG 377
S++G LL KLA L E+ A LA +E +D+GKP V A +V ++RY AG ADKI G
Sbjct: 78 SEKGELLMKLAELTEKHADTLAAIEAMDSGKPLVSNARGDVDGTIALLRYCAGWADKIYG 137
Query: 378 NTIPADGEVLTFTLKEPV 431
IP E L + + P+
Sbjct: 138 QVIPTGPEKLAYAKRTPI 155
Score = 63.3 bits (147), Expect = 2e-11
Identities = 26/39 (66%), Positives = 31/39 (79%)
Frame = +2
Query: 434 VCGQILPWNYPIPMFIWNIAPALAAGCTVVVKPAEQTPL 550
VCGQI+PWNYP+ M W IAPALAAG +++K AE TPL
Sbjct: 157 VCGQIVPWNYPLNMAGWKIAPALAAGNCIIIKSAETTPL 195
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 73.7 bits (173), Expect = 2e-14
Identities = 43/149 (28%), Positives = 74/149 (49%), Gaps = 2/149 (1%)
Frame = +3
Query: 3 DIKYTKLFINNEWVDAVSKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSE 182
D + + F+ +W+ + + KTF NP +I +VA+ F Y
Sbjct: 14 DKSHAQSFVQGKWISSPNNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAFKTYKN 73
Query: 183 WRLLDASQRGLLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKA 362
+ + SQ LL + A L+ + L ++ TL+NGKP+ QAE EV SG +++YA +A
Sbjct: 74 FTHVQRSQ---LLERWAELIMENKDDLVKMLTLENGKPLSQAEMEVTTCSGYLKWYAAEA 130
Query: 363 DKILGNTIPADGEVLTF--TLKEPVVFAA 443
+ G+ P+ + F ++K+PV +A
Sbjct: 131 VRTFGDVAPSSLQSQNFLISIKQPVGVSA 159
Score = 40.3 bits (90), Expect = 2e-04
Identities = 20/46 (43%), Positives = 24/46 (52%)
Frame = +2
Query: 410 IYLKGTRRVCGQILPWNYPIPMFIWNIAPALAAGCTVVVKPAEQTP 547
I +K V I PWN+P M ALAAGCT + PA +TP
Sbjct: 149 ISIKQPVGVSALITPWNFPAAMIARKGGAALAAGCTAIFLPAFRTP 194
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 59.3 bits (137), Expect = 4e-10
Identities = 39/137 (28%), Positives = 68/137 (49%), Gaps = 1/137 (0%)
Frame = +3
Query: 24 FINNEWVDAVSKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLLDAS 203
+I +WV A S KTF NP + V + F Y R D
Sbjct: 27 YIGGKWVTAASGKTFDVENPGLNETLAPVTDMSVEETRKAIKVAHEAFLSY---RNSDIK 83
Query: 204 QRGLLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADKILG-N 380
+R +L + L+ +A LA + TL+NGK + A+ EV++A+ + ++AG+A +I G +
Sbjct: 84 ERYAILRRWYDLIMENADDLATMMTLENGKALGDAKGEVVYAAKFIDWFAGEALRISGDS 143
Query: 381 TIPADGEVLTFTLKEPV 431
++ ++ + T+K+PV
Sbjct: 144 SMSSNPQNRIITIKQPV 160
Score = 51.6 bits (118), Expect = 7e-08
Identities = 23/46 (50%), Positives = 29/46 (63%)
Frame = +2
Query: 410 IYLKGTRRVCGQILPWNYPIPMFIWNIAPALAAGCTVVVKPAEQTP 547
I +K V G I PWN+P M + ALAAGCTVV++PA +TP
Sbjct: 154 ITIKQPVGVVGIITPWNFPAAMITRKVGAALAAGCTVVIRPAAETP 199
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 52.8 bits (121), Expect = 3e-08
Identities = 34/117 (29%), Positives = 53/117 (45%), Gaps = 2/117 (1%)
Frame = +3
Query: 21 LFINNEWVDAV--SKKTFPTINPQDESVIVQVAEGXXXXXXXXXXXXXXXFHRYSEWRLL 194
LFI+ ++V + + K P INP E +I A F R W
Sbjct: 23 LFIDGKFVSPIEPAAKPIPLINPATEEIIGTCANASAKDVDSAVENAYNTF-RSGIWAKW 81
Query: 195 DASQRGLLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKAD 365
QRGL+L K+A +M + LA ++T++ GKP A ++ + + YYA A+
Sbjct: 82 PGKQRGLVLRKIAKMMREKRELLAGIDTINCGKPTPYALFDIDSCADMFEYYAEVAE 138
Score = 51.6 bits (118), Expect = 7e-08
Identities = 20/40 (50%), Positives = 28/40 (70%)
Frame = +2
Query: 428 RRVCGQILPWNYPIPMFIWNIAPALAAGCTVVVKPAEQTP 547
R V G I PWN+P+ M +W + PA+A+G VV+KP+E P
Sbjct: 163 RGVIGVITPWNFPLKMALWKLVPAIASGNCVVLKPSELAP 202
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 31.1 bits (67), Expect = 0.11
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +2
Query: 434 VCGQILPWNYPIPMFIWNIAPALAAGCTVVVKPAEQT 544
V ++ WNYP+ + I AL AG +VVK +E T
Sbjct: 130 VIAALVSWNYPLHNALGPIISALFAGNAIVVKGSELT 166
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 25.4 bits (53), Expect = 5.6
Identities = 10/27 (37%), Positives = 16/27 (59%)
Frame = +3
Query: 297 VKQAEQEVLWASGIVRYYAGKADKILG 377
VK+ + +W G + YAG+ +ILG
Sbjct: 219 VKKGDTVAIWGMGPIGLYAGRWAQILG 245
>SPAC12G12.16c ||SPAC18B11.01c|nuclease, XP-G
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 496
Score = 25.0 bits (52), Expect = 7.4
Identities = 16/36 (44%), Positives = 21/36 (58%), Gaps = 2/36 (5%)
Frame = +3
Query: 201 SQRGLLLFKLATLMERDAKYLAE--LETLDNGKPVK 302
SQ G LLF L +L ERD + A+ E L+N V+
Sbjct: 143 SQIGKLLFDLRSLNERDNPFHAQNNAENLENATFVR 178
>SPCC1672.09 |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 467
Score = 25.0 bits (52), Expect = 7.4
Identities = 7/14 (50%), Positives = 11/14 (78%)
Frame = -2
Query: 308 CLLHWFSVVQCFEF 267
CL+HWF +++ EF
Sbjct: 337 CLVHWFQIMRSAEF 350
>SPBC543.03c |pku80||Ku domain protein Pku80|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 695
Score = 24.6 bits (51), Expect = 9.8
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 2/74 (2%)
Frame = +3
Query: 213 LLLFKLATLMERDAKYLAELETLDNGKPVKQAEQEVLWASGIVRYYAGKADKILGNTIPA 392
L+ KL + KY ++ D G P+ + + E L SG+ + +K+L +
Sbjct: 531 LIKEKLTVNVPVKPKYSSQETAFDTGAPISEEQIEELLNSGLDEQ---EGEKLLVLHVSE 587
Query: 393 DGEVLTFT--LKEP 428
V TFT LK P
Sbjct: 588 KDPVGTFTEVLKNP 601
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,259,606
Number of Sequences: 5004
Number of extensions: 43165
Number of successful extensions: 106
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 98
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 103
length of database: 2,362,478
effective HSP length: 69
effective length of database: 2,017,202
effective search space used: 229961028
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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