Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= I10A02NGRL0001_L21
(463 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g57550.1 68416.m06408 guanylate kinase 2 (GK-2) identical to ... 66 1e-11
At2g41880.1 68415.m05179 guanylate kinase 1 (GK-1) identical to ... 64 4e-11
At3g06200.1 68416.m00713 guanylate kinase, putative similar to g... 48 2e-06
At3g31310.1 68416.m03983 hypothetical protein 32 0.22
At1g10390.1 68414.m01171 nucleoporin family protein contains Pfa... 29 1.2
At5g17880.1 68418.m02097 disease resistance protein (TIR-NBS-LRR... 29 2.0
At5g03450.1 68418.m00300 zinc finger (C3HC4-type RING finger) fa... 27 4.7
At4g35910.1 68417.m05103 expressed protein 27 4.7
At3g59100.1 68416.m06589 glycosyl transferase family 48 protein ... 27 4.7
At5g01140.1 68418.m00018 hypothetical protein contains Pfam prof... 27 6.2
At3g55950.1 68416.m06217 protein kinase family protein contains ... 27 6.2
At2g40040.1 68415.m04920 defective chloroplasts and leaves prote... 27 6.2
At2g21250.1 68415.m02526 mannose 6-phosphate reductase (NADPH-de... 27 6.2
At5g35430.1 68418.m04213 expressed protein 27 8.2
At2g21260.1 68415.m02530 mannose 6-phosphate reductase (NADPH-de... 27 8.2
At1g60700.1 68414.m06833 forkhead-associated domain-containing p... 27 8.2
>At3g57550.1 68416.m06408 guanylate kinase 2 (GK-2) identical to
guanylate kinase (GK-2) [Arabidopsis thaliana]
gi|7861798|gb|AAF70409
Length = 389
Score = 65.7 bits (153), Expect = 1e-11
Identities = 29/72 (40%), Positives = 44/72 (61%)
Frame = +3
Query: 174 LALVGTRGVGRRTLKNRLIQEYPDRFGAVIPHTCRPPRPMEENGQSYWFVSREEMERDAH 353
+ + G GVG+ TL + L++E+P FG + HT R PR ME+NG Y F + ME++
Sbjct: 140 IVISGPSGVGKGTLISMLMKEFPSMFGFSVSHTTRAPRCMEKNGVHYHFTDKTVMEKEIK 199
Query: 354 AGRFLEYGEHNG 389
G+FLE+ +G
Sbjct: 200 DGKFLEFASVHG 211
Score = 35.9 bits (79), Expect = 0.013
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +1
Query: 388 GNLYGTHLDSIRAVIKQGKMCILD 459
GNLYGT ++S+ V GK CILD
Sbjct: 211 GNLYGTSIESVEVVTDSGKRCILD 234
>At2g41880.1 68415.m05179 guanylate kinase 1 (GK-1) identical to
guanylate kinase (GK-1) [Arabidopsis thaliana]
gi|7861795|gb|AAF70408
Length = 387
Score = 64.1 bits (149), Expect = 4e-11
Identities = 28/72 (38%), Positives = 44/72 (61%)
Frame = +3
Query: 174 LALVGTRGVGRRTLKNRLIQEYPDRFGAVIPHTCRPPRPMEENGQSYWFVSREEMERDAH 353
+ + G GVG+ TL + L++E+P FG + HT R PR ME +G Y F ++ ME++
Sbjct: 140 IVISGPSGVGKGTLISMLMKEFPSMFGFSVSHTTRSPRSMEMDGVHYHFADKKVMEKEIK 199
Query: 354 AGRFLEYGEHNG 389
G+FLE+ +G
Sbjct: 200 DGKFLEFASVHG 211
Score = 37.5 bits (83), Expect = 0.004
Identities = 15/24 (62%), Positives = 18/24 (75%)
Frame = +1
Query: 388 GNLYGTHLDSIRAVIKQGKMCILD 459
GNLYGT ++S+ AV GK CILD
Sbjct: 211 GNLYGTSIESVEAVTDSGKRCILD 234
>At3g06200.1 68416.m00713 guanylate kinase, putative similar to
guanylate kinase (GMP kinase) [Bacillus halodurans]
Swiss-Prot:Q9K9Y2; contains Pfam profile: PF00625
guanylate kinase
Length = 282
Score = 48.4 bits (110), Expect = 2e-06
Identities = 30/85 (35%), Positives = 47/85 (55%), Gaps = 4/85 (4%)
Frame = +3
Query: 153 PPFLRRVLALVGTRGVGRRTLKNRLIQEYPDRFGAVIPHTCRPPRPMEENGQSYWFVSRE 332
PP + V+ + G GVG+ + N+L +E + V+ T RP RP E +G+ Y+FVSR+
Sbjct: 84 PPPNQIVIVISGPSGVGKDAVINKL-REVREGLHFVVTATSRPMRPGEVDGKDYFFVSRD 142
Query: 333 E----MERDAHAGRFLEYGEHNGKP 395
+ +E + L YGE+ G P
Sbjct: 143 QFLSMVENEELLEYALVYGEYKGIP 167
>At3g31310.1 68416.m03983 hypothetical protein
Length = 291
Score = 31.9 bits (69), Expect = 0.22
Identities = 15/31 (48%), Positives = 20/31 (64%), Gaps = 3/31 (9%)
Frame = -1
Query: 97 ERDSYTNFFFFF---DILAPHMLILCTKSAS 14
+ +SYTN+FFF D P L+LC K+AS
Sbjct: 165 KNESYTNYFFFVALDDATIPEDLLLCDKAAS 195
>At1g10390.1 68414.m01171 nucleoporin family protein contains Pfam
profiles: PF04096 nucleoporin autopeptidase, PF03093
nucleoporin FG repeat family
Length = 1041
Score = 29.5 bits (63), Expect = 1.2
Identities = 12/35 (34%), Positives = 21/35 (60%)
Frame = +3
Query: 207 RTLKNRLIQEYPDRFGAVIPHTCRPPRPMEENGQS 311
R L R +Q++ R +++P RP P+ +NG+S
Sbjct: 770 RALVIRPVQQWSSRDKSILPKEQRPTAPLHDNGKS 804
>At5g17880.1 68418.m02097 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1197
Score = 28.7 bits (61), Expect = 2.0
Identities = 11/28 (39%), Positives = 18/28 (64%)
Frame = +3
Query: 168 RVLALVGTRGVGRRTLKNRLIQEYPDRF 251
R + +VG G+G+ TL L +++ DRF
Sbjct: 227 RTIGVVGMPGIGKTTLATMLYEKWNDRF 254
>At5g03450.1 68418.m00300 zinc finger (C3HC4-type RING finger)
family protein
Length = 630
Score = 27.5 bits (58), Expect = 4.7
Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = -1
Query: 283 GGRH-VCGMTAPNLSGYSCMSRFLRVRLPTPRVP 185
GG+H VC + +L GYSC++++ + R + P
Sbjct: 130 GGQHQVCCLPCGHLYGYSCINKWFQQRRSGGKCP 163
>At4g35910.1 68417.m05103 expressed protein
Length = 458
Score = 27.5 bits (58), Expect = 4.7
Identities = 14/32 (43%), Positives = 18/32 (56%)
Frame = +1
Query: 1 LMYHLKRTSCIRSAYAERGYRRRKRSSCTSLV 96
L+ HLK S ++ AE GY R SCTS +
Sbjct: 204 LLLHLKMLS-LQKVAAENGYNRLVLGSCTSRI 234
>At3g59100.1 68416.m06589 glycosyl transferase family 48 protein
contains Pfam profile: PF02364 1,3-beta-glucan synthase
Length = 1934
Score = 27.5 bits (58), Expect = 4.7
Identities = 10/28 (35%), Positives = 19/28 (67%)
Frame = +3
Query: 288 PMEENGQSYWFVSREEMERDAHAGRFLE 371
P+E++ +S+W V +E ++ + GR LE
Sbjct: 1719 PVEKSWESWWNVEQEHLKHTSIRGRILE 1746
>At5g01140.1 68418.m00018 hypothetical protein contains Pfam profile
PF05056: Protein of unknown function (DUF674)
Length = 473
Score = 27.1 bits (57), Expect = 6.2
Identities = 9/17 (52%), Positives = 12/17 (70%)
Frame = -1
Query: 238 YSCMSRFLRVRLPTPRV 188
Y C + L++R PTPRV
Sbjct: 318 YRCQKQLLKIRTPTPRV 334
>At3g55950.1 68416.m06217 protein kinase family protein contains
protein kinase domain, Pfam:PF00069; similar to
cytokinin-regulated kinase 1 [Nicotiana tabacum]
gi|10998537|gb|AAG25966
Length = 814
Score = 27.1 bits (57), Expect = 6.2
Identities = 16/40 (40%), Positives = 21/40 (52%), Gaps = 2/40 (5%)
Frame = -1
Query: 301 FSSIGRGGRHVCGMTAPNLSGYSCM--SRFLRVRLPTPRV 188
F SI G +CG+ + NLS C + F R+ LP P V
Sbjct: 282 FESISSGPGLICGLISSNLS-IMCWNPTNFSRIFLPFPEV 320
>At2g40040.1 68415.m04920 defective chloroplasts and leaves
protein-related / DCL protein-related similar to DCL
protein, chloroplast precursor (Defective chloroplasts
and leaves protein) (Swiss-Prot:Q42463) [Lycopersicon
esculentum]
Length = 839
Score = 27.1 bits (57), Expect = 6.2
Identities = 12/29 (41%), Positives = 17/29 (58%)
Frame = +3
Query: 204 RRTLKNRLIQEYPDRFGAVIPHTCRPPRP 290
R++L N L+++YPDR I PRP
Sbjct: 687 RKSLNNYLMKKYPDRAEEFIDKYFTKPRP 715
>At2g21250.1 68415.m02526 mannose 6-phosphate reductase
(NADPH-dependent), putative 6-phosphate reductase [Apium
graveolens][GI:1835701], NADP-dependent
D-sorbitol-6-phosphate dehydrogenase [Malus
domestica][SP|P28475]
Length = 309
Score = 27.1 bits (57), Expect = 6.2
Identities = 17/47 (36%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Frame = +3
Query: 204 RRTLKNRLIQEYPDRFGAVIPHTCRPPRPMEENGQSYWF-VSREEME 341
++T+ +++ R VIP T +P R +EEN Q + F +S+E+ME
Sbjct: 240 KKTVAQVVLRWGIQRKTVVIPKTSKPAR-LEENFQVFDFELSKEDME 285
>At5g35430.1 68418.m04213 expressed protein
Length = 786
Score = 26.6 bits (56), Expect = 8.2
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = -3
Query: 293 HRPRRTTRVWYDSTESIRIFLYESVLEGAPADAARAHKRQHTPQEGRSPRHLLVE 129
H RR +W E + L + +LEG + R+ R H +G + R L++E
Sbjct: 439 HVFRRQPLIWLRLAECCMMALQKGLLEGGNSSLDRSEIRVHVIGKG-NRRQLMIE 492
>At2g21260.1 68415.m02530 mannose 6-phosphate reductase
(NADPH-dependent), putative similar to NADPH-dependent
mannose 6-phosphate reductase [Apium
graveolens][GI:1835701], NADP-dependent
D-sorbitol-6-phosphate dehydrogenase [Malus
domestica][SP|P28475]
Length = 309
Score = 26.6 bits (56), Expect = 8.2
Identities = 15/29 (51%), Positives = 21/29 (72%), Gaps = 1/29 (3%)
Frame = +3
Query: 258 VIPHTCRPPRPMEENGQSYWF-VSREEME 341
VIP T +P R +EEN Q + F +S+E+ME
Sbjct: 258 VIPKTSKPER-LEENFQVFDFQLSKEDME 285
>At1g60700.1 68414.m06833 forkhead-associated domain-containing
protein / FHA domain-containing protein contains
similarity to nucleolar protein GI:2384719 from [Mus
musculus]
Length = 525
Score = 26.6 bits (56), Expect = 8.2
Identities = 21/80 (26%), Positives = 30/80 (37%)
Frame = -3
Query: 260 DSTESIRIFLYESVLEGAPADAARAHKRQHTPQEGRSPRHLLVERELCPLQRHRRTRLVH 81
D E I I L P D+ R+ + PRH L+ E C +R + H
Sbjct: 339 DGEEEIDIDAMIRKLNLVPDDSDSCFNREEWNMS-KHPRHALIGLEQCTRTSMQRAIMFH 397
Query: 80 ELLFLLRYPRSAYADLMHEV 21
+ +L P S + EV
Sbjct: 398 GAIAVLHCPDSKHFVRKREV 417
Database: arabidopsis
Posted date: Oct 4, 2007 10:56 AM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 9,331,564
Number of Sequences: 28952
Number of extensions: 172249
Number of successful extensions: 598
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 584
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 597
length of database: 12,070,560
effective HSP length: 75
effective length of database: 9,899,160
effective search space used: 772134480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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