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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= I10A02NGRL0001_L02
         (576 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g49310.1 68418.m06102 importin alpha-1 subunit, putative simi...    32   0.24 
At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2) sim...    31   0.55 
At4g28600.1 68417.m04090 calmodulin-binding protein similar to p...    30   0.96 
At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMP...    30   0.96 
At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMP...    30   0.96 
At4g26250.1 68417.m03778 galactinol synthase, putative similar t...    29   2.2  
At3g05720.1 68416.m00640 importin alpha-1 subunit, putative simi...    28   3.9  
At5g52000.1 68418.m06453 importin alpha-1 subunit, putative simi...    28   5.1  
At5g23790.1 68418.m02793 galactinol synthase, putative similar t...    28   5.1  
At3g08810.1 68416.m01023 kelch repeat-containing F-box family pr...    28   5.1  
At1g09270.2 68414.m01036 importin alpha-1 subunit, putative (IMP...    28   5.1  
At1g09270.1 68414.m01035 importin alpha-1 subunit, putative (IMP...    28   5.1  
At4g02150.1 68417.m00287 importin alpha-2 subunit identical to i...    27   9.0  
At1g01950.1 68414.m00113 armadillo/beta-catenin repeat family pr...    27   9.0  

>At5g49310.1 68418.m06102 importin alpha-1 subunit, putative similar
           to importin alpha-1 subunit (Karyopherin alpha-1
           subunit, KAP alpha) [Arabidopsis thaliana]
           SWISS-PROT:Q96321
          Length = 519

 Score = 32.3 bits (70), Expect = 0.24
 Identities = 22/54 (40%), Positives = 30/54 (55%)
 Frame = +3

Query: 399 AVWALANLTTVYPEKYCSLVEAEGGLKLLNDLLVHPAPYHLIKKLAWTVIGNCV 560
           A WAL+NL+    E   S++EA G +  L +LL H +P  L+  L    IGN V
Sbjct: 260 ACWALSNLSDASNENIQSVIEA-GVVPRLVELLQHASPVVLVPAL--RCIGNIV 310



 Score = 27.9 bits (59), Expect = 5.1
 Identities = 18/67 (26%), Positives = 32/67 (47%), Gaps = 1/67 (1%)
 Frame = +3

Query: 348 PILG-LLRAYHTPQCQHWAVWALANLTTVYPEKYCSLVEAEGGLKLLNDLLVHPAPYHLI 524
           P+L  LL   H    +  A W ++N+T    E+  S+++A     L+N  L   A + + 
Sbjct: 327 PVLADLLTQNHMRGIRREACWTISNITAGLEEQIQSVIDANLIPSLVN--LAQHAEFDIK 384

Query: 525 KKLAWTV 545
           K+  W +
Sbjct: 385 KEAIWAI 391


>At4g16143.1 68417.m02447 importin alpha-2, putative (IMPA-2)
           similar to importin alpha 2 [Capsicum annuum]
           GI:13752562; contains Pfam profiles PF01749: Importin
           beta binding domain, PF00514:
           Armadillo/beta-catenin-like repeat; non-consensus GG
           donor splice site at exon 1 and 6; CT acceptor splice
           site at exon 2
          Length = 444

 Score = 31.1 bits (67), Expect = 0.55
 Identities = 20/80 (25%), Positives = 39/80 (48%)
 Frame = +3

Query: 306 DLHAERNINYRSFEPILGLLRAYHTPQCQHWAVWALANLTTVYPEKYCSLVEAEGGLKLL 485
           DL  +  I++ +   +L LL   H    +  A W ++N+T    ++  ++ EA     L+
Sbjct: 321 DLQTQCVISHGALLSLLSLLTHNHKKSIKKEACWTISNITAGNRDQIQAVCEAGLICPLV 380

Query: 486 NDLLVHPAPYHLIKKLAWTV 545
           N  L+  A + + K+ AW +
Sbjct: 381 N--LLQNAEFDIKKEAAWAI 398


>At4g28600.1 68417.m04090 calmodulin-binding protein similar to
           pollen-specific calmodulin-binding protein MPCBP
           GI:10086260 from [Zea mays]
          Length = 739

 Score = 30.3 bits (65), Expect = 0.96
 Identities = 16/61 (26%), Positives = 25/61 (40%)
 Frame = +3

Query: 138 LVFYELLESSSDGIEVSYNAAGVLVHMASDGPDAWCIDDPPRELVLNRVAAAVERWDLHA 317
           +V   L E +SD +         L       P+ W + D PR+ +L+   A +  W L  
Sbjct: 191 IVETSLAEGASDNVTGDIKLQETLTKAVELLPELWKLADSPRDAILSYRRALLNHWKLDP 250

Query: 318 E 320
           E
Sbjct: 251 E 251


>At3g06720.2 68416.m00797 importin alpha-1 subunit, putative (IMPA1)
           similar to importin alpha-1 subunit (Karyopherin alpha-1
           subunit, KAP alpha) [Arabidopsis thaliana]
           SWISS-PROT:Q96321
          Length = 532

 Score = 30.3 bits (65), Expect = 0.96
 Identities = 22/91 (24%), Positives = 41/91 (45%)
 Frame = +3

Query: 273 LNRVAAAVERWDLHAERNINYRSFEPILGLLRAYHTPQCQHWAVWALANLTTVYPEKYCS 452
           L  V   V   D+  +  IN  +   +  LL   H    +  A W ++N+T    ++  +
Sbjct: 305 LRTVGNIVTGDDIQTQCVINSGALPCLANLLTQNHKKSIKKEACWTISNITAGNKDQIQT 364

Query: 453 LVEAEGGLKLLNDLLVHPAPYHLIKKLAWTV 545
           +VEA     L++  L+  A + + K+ AW +
Sbjct: 365 VVEANLISPLVS--LLQNAEFDIKKEAAWAI 393



 Score = 27.1 bits (57), Expect = 9.0
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +3

Query: 399 AVWALANLTTVYPEKYCSLVEAEGGLKLLNDLLVHPAP 512
           A WA++N T+         +  +G +K L DLLV P P
Sbjct: 389 AAWAISNATSGGSHDQIKYLVEQGCIKPLCDLLVCPDP 426


>At3g06720.1 68416.m00796 importin alpha-1 subunit, putative (IMPA1)
           similar to importin alpha-1 subunit (Karyopherin alpha-1
           subunit, KAP alpha) [Arabidopsis thaliana]
           SWISS-PROT:Q96321
          Length = 532

 Score = 30.3 bits (65), Expect = 0.96
 Identities = 22/91 (24%), Positives = 41/91 (45%)
 Frame = +3

Query: 273 LNRVAAAVERWDLHAERNINYRSFEPILGLLRAYHTPQCQHWAVWALANLTTVYPEKYCS 452
           L  V   V   D+  +  IN  +   +  LL   H    +  A W ++N+T    ++  +
Sbjct: 305 LRTVGNIVTGDDIQTQCVINSGALPCLANLLTQNHKKSIKKEACWTISNITAGNKDQIQT 364

Query: 453 LVEAEGGLKLLNDLLVHPAPYHLIKKLAWTV 545
           +VEA     L++  L+  A + + K+ AW +
Sbjct: 365 VVEANLISPLVS--LLQNAEFDIKKEAAWAI 393



 Score = 27.1 bits (57), Expect = 9.0
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +3

Query: 399 AVWALANLTTVYPEKYCSLVEAEGGLKLLNDLLVHPAP 512
           A WA++N T+         +  +G +K L DLLV P P
Sbjct: 389 AAWAISNATSGGSHDQIKYLVEQGCIKPLCDLLVCPDP 426


>At4g26250.1 68417.m03778 galactinol synthase, putative similar to
           galactinol synthase, isoform GolS-1 [Ajuga reptans]
           GI:5608497; contains Pfam profile: PF01501 glycosyl
           transferase family 8
          Length = 336

 Score = 29.1 bits (62), Expect = 2.2
 Identities = 9/47 (19%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
 Frame = +3

Query: 207 LVHMASDGPDAWCIDDPPRELVLNRVAAAVER-WDLHAERNINYRSF 344
           ++H  ++G   W  D+    +    +   V++ WD++ + +++Y++F
Sbjct: 258 VIHYCANGSKPWRFDETEEHMDREDIKMLVKKWWDIYEDSSLDYKNF 304


>At3g05720.1 68416.m00640 importin alpha-1 subunit, putative similar
           to importin alpha subunit (Karyopherin alpha subunit)
           (KAP alpha) SP:O22478 from [Lycopersicon esculentum]
          Length = 528

 Score = 28.3 bits (60), Expect = 3.9
 Identities = 18/59 (30%), Positives = 30/59 (50%)
 Frame = +3

Query: 378 TPQCQHWAVWALANLTTVYPEKYCSLVEAEGGLKLLNDLLVHPAPYHLIKKLAWTVIGN 554
           +PQ Q  A WAL N+ +   E    +V   G + +L  LL   +PY ++++     +GN
Sbjct: 117 SPQLQFEAAWALTNIASGTSEN-TEVVIDHGAVAILVRLL--NSPYDVVREQVVWALGN 172


>At5g52000.1 68418.m06453 importin alpha-1 subunit, putative similar
           to importin alpha-1 subunit (Karyopherin alpha-1
           subunit, KAP alpha) [Arabidopsis thaliana]
           SWISS-PROT:Q96321
          Length = 441

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 17/73 (23%), Positives = 34/73 (46%)
 Frame = +3

Query: 327 INYRSFEPILGLLRAYHTPQCQHWAVWALANLTTVYPEKYCSLVEAEGGLKLLNDLLVHP 506
           IN  +   I  +L   H  + +  A W ++N+T    E+  S+++A     L+N  L   
Sbjct: 251 INSGALPIISNMLTRNHENKIKKCACWVISNITAGTKEQIQSVIDANLIPILVN--LAQD 308

Query: 507 APYHLIKKLAWTV 545
             +++ K+  W +
Sbjct: 309 TDFYMKKEAVWAI 321


>At5g23790.1 68418.m02793 galactinol synthase, putative similar to
           galactinol synthase, isoform GolS-1 GI:5608497 from
           [Ajuga reptans]; contains Pfam profile: PF01501 glycosyl
           transferase family 8
          Length = 333

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 9/47 (19%), Positives = 26/47 (55%), Gaps = 1/47 (2%)
 Frame = +3

Query: 207 LVHMASDGPDAWCIDDPPRELVLNRVAAAVER-WDLHAERNINYRSF 344
           +VH  ++G   W  D+    +    +   V++ W+++ + +++Y++F
Sbjct: 255 VVHYCANGSKPWKFDEAEEHMDREDIKMLVKKWWEIYEDSSLDYKNF 301


>At3g08810.1 68416.m01023 kelch repeat-containing F-box family
           protein contains F-box domain Pfam:PF00646 and Kelch
           motif Pfam:PF01344
          Length = 343

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 22/59 (37%), Positives = 32/59 (54%), Gaps = 1/59 (1%)
 Frame = +3

Query: 372 YHTPQCQHWAVWALANLTT-VYPEKYCSLVEAEGGLKLLNDLLVHPAPYHLIKKLAWTV 545
           + T QC   AV AL   T+ V+ EK C +    G ++ ++DLL  P  Y L+K L  T+
Sbjct: 291 HKTIQC---AVIALERRTSFVFREKSCQIW---GKVEWVDDLLTVPTSYDLVKVLVATL 343


>At1g09270.2 68414.m01036 importin alpha-1 subunit, putative (IMPA4)
           similar to importin alpha-1 subunit (Karyopherin alpha-1
           subunit, KAP alpha) [Arabidopsis thaliana]
           SWISS-PROT:Q96321
          Length = 538

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = +3

Query: 399 AVWALANLTTVYPEKYCSLVEAEGGLKLLNDLLVHPAP 512
           A WA++N T+    +    +  +G +K L DLL+ P P
Sbjct: 396 AAWAISNATSGGSHEQIQYLVTQGCIKPLCDLLICPDP 433


>At1g09270.1 68414.m01035 importin alpha-1 subunit, putative (IMPA4)
           similar to importin alpha-1 subunit (Karyopherin alpha-1
           subunit, KAP alpha) [Arabidopsis thaliana]
           SWISS-PROT:Q96321
          Length = 538

 Score = 27.9 bits (59), Expect = 5.1
 Identities = 13/38 (34%), Positives = 21/38 (55%)
 Frame = +3

Query: 399 AVWALANLTTVYPEKYCSLVEAEGGLKLLNDLLVHPAP 512
           A WA++N T+    +    +  +G +K L DLL+ P P
Sbjct: 396 AAWAISNATSGGSHEQIQYLVTQGCIKPLCDLLICPDP 433


>At4g02150.1 68417.m00287 importin alpha-2 subunit identical to
           importin alpha-2 subunit (Karyopherin alpha-2 subunit)
           (KAP alpha) SP:O04294 from [Arabidopsis thaliana]
          Length = 531

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 17/80 (21%), Positives = 42/80 (52%)
 Frame = +3

Query: 306 DLHAERNINYRSFEPILGLLRAYHTPQCQHWAVWALANLTTVYPEKYCSLVEAEGGLKLL 485
           DL  +  ++ ++   +L LL+  +    +  A W ++N+T    ++  ++++A G ++ L
Sbjct: 319 DLQTQMVLDQQALPCLLNLLKNNYKKSIKKEACWTISNITAGNADQIQAVIDA-GIIQSL 377

Query: 486 NDLLVHPAPYHLIKKLAWTV 545
             +L   A + + K+ AW +
Sbjct: 378 VWVL-QSAEFEVKKEAAWGI 396


>At1g01950.1 68414.m00113 armadillo/beta-catenin repeat family
           protein / kinesin motor family protein similar to
           kinesin-like protein GB:CAB41097 GI:5541717 from
           [Arabidopsis thaliana]; contains Pfam profiles PF00225:
           Kinesin motor domain, PF00514:
           Armadillo/beta-catenin-like repeat
          Length = 894

 Score = 27.1 bits (57), Expect = 9.0
 Identities = 21/80 (26%), Positives = 36/80 (45%)
 Frame = +3

Query: 249 DDPPRELVLNRVAAAVERWDLHAERNINYRSFEPILGLLRAYHTPQCQHWAVWALANLTT 428
           DD    +   +V A +   + + E+ +       +L LLR+Y     +  A  A+ANL  
Sbjct: 648 DDANIRIHAVKVVANLAAEEANQEKIVEAGGLTSLLMLLRSYEDETVRRVAAGAIANL-- 705

Query: 429 VYPEKYCSLVEAEGGLKLLN 488
              E    L+  +GG+ LL+
Sbjct: 706 AMNEVSQQLIVDQGGISLLS 725


  Database: arabidopsis
    Posted date:  Oct 4, 2007 10:56 AM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,274,882
Number of Sequences: 28952
Number of extensions: 323634
Number of successful extensions: 820
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 772
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 820
length of database: 12,070,560
effective HSP length: 77
effective length of database: 9,841,256
effective search space used: 1121903184
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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