BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_K23 (593 letters) Database: spombe 5004 sequences; 2,362,478 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 133 2e-32 SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 73 3e-14 SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 66 5e-12 SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 45 1e-05 SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 41 1e-04 SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 41 1e-04 SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 39 6e-04 SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 36 0.006 SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 28 0.89 SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Sch... 28 1.2 SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptid... 27 1.6 SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogam... 27 2.1 SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces ... 26 3.6 SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomy... 26 4.8 SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 25 6.3 SPBC215.08c |arg4||carbamoyl-phosphate synthase Arg4|Schizosacch... 25 6.3 SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyce... 25 8.3 >SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 492 Score = 133 bits (322), Expect = 2e-32 Identities = 66/153 (43%), Positives = 97/153 (63%), Gaps = 1/153 (0%) Frame = +1 Query: 55 LVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTLK 234 +V FYAPWCGHCK L PEY AA L+KD +SL +VDCTE G C ++S+ GYPTL Sbjct: 43 MVKFYAPWCGHCKALAPEYESAADELEKDG--ISLVEVDCTEEG-DLCSEYSIRGYPTLN 99 Query: 235 IFRKGELSSDYNGPRESNGIVKYMRAQVGPSSRDLLTLADFEAFTAK-DDVVVIGFFEKE 411 +F+ G+ S Y+GPR+ + +VKYMR Q+ P+ + ++ E F K DD+ V+ FF K+ Sbjct: 100 VFKNGKQISQYSGPRKHDALVKYMRKQLLPTVKP-ISKDTLENFVEKADDLAVVAFF-KD 157 Query: 412 SDLKGDFLKTADKMREEVTFGHSSAKEVLEKVG 510 L + + A+ M+++ F S KE+ + +G Sbjct: 158 QKLNDTYTEVAEVMKDDFVFAASDDKELAKSLG 190 Score = 65.3 bits (152), Expect = 6e-12 Identities = 33/74 (44%), Positives = 42/74 (56%) Frame = +1 Query: 31 VLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFS 210 V+D+ LV FYAPWCGHCK L P Y A DD V +AK+D TE S S Sbjct: 369 VMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEY-SDDSNVVVAKIDATENDISV----S 423 Query: 211 VSGYPTLKIFRKGE 252 +SG+PT+ F+ + Sbjct: 424 ISGFPTIMFFKAND 437 >SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 363 Score = 72.9 bits (171), Expect = 3e-14 Identities = 41/121 (33%), Positives = 64/121 (52%), Gaps = 6/121 (4%) Frame = +1 Query: 1 LDLTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDC-T 177 ++L +F + +LV+FYAPWCG+CK+L P Y A L P + VDC Sbjct: 34 IELNSKNFRKFVKAKGPSLVVFYAPWCGYCKKLVPTYQKLASNLHSLLP---VTAVDCDA 90 Query: 178 EGGKSTCEKFSVSGYPTLKIF---RKGE--LSSDYNGPRESNGIVKYMRAQVGPSSRDLL 342 + ++ C ++ V G+PT+K+ KG S+DYNG R + K++ + PS +L Sbjct: 91 DQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSI-PSKVKIL 149 Query: 343 T 345 T Sbjct: 150 T 150 >SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces pombe|chr 1|||Manual Length = 359 Score = 65.7 bits (153), Expect = 5e-12 Identities = 49/184 (26%), Positives = 77/184 (41%), Gaps = 3/184 (1%) Frame = +1 Query: 31 VLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFS 210 V+D LV FYA WCG+CKRL P Y G + K++P V + K++ + Sbjct: 154 VMDDKKDVLVEFYADWCGYCKRLAPTYE-TLGKVFKNEPNVEIVKIN-ADVFADIGRLHE 211 Query: 211 VSGYPTLKIFRKGELSSD--YNGPRESNGIVKYMRAQVGPSSRDLLTLADFEAFTAKDDV 384 V+ +PT+K F K + Y G R +++Y+ + G TL D Sbjct: 212 VASFPTIKFFPKDDKDKPELYEGDRSLESLIEYINKKSGTQRSPDGTLLSTAGRIPTFDE 271 Query: 385 VVIGFFEKESDLKGDFLKTADKMR-EEVTFGHSSAKEVLEKVGYKNNVVLYRPKRLQNKF 561 F + + K L+ ++ E+ + K+V EK+ N V KRL Sbjct: 272 FAAEFLDMSNAAKEVVLEKVKQLALEDSSRWTKYYKKVFEKILNDENWVHKEAKRLSKLL 331 Query: 562 EESS 573 + S Sbjct: 332 RQKS 335 Score = 60.5 bits (140), Expect = 2e-10 Identities = 31/98 (31%), Positives = 51/98 (52%), Gaps = 1/98 (1%) Frame = +1 Query: 52 ALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTL 231 AL+ FYA WCGHCK L P Y G L +D V + K+D + +K+ ++G+PTL Sbjct: 42 ALIEFYATWCGHCKSLAPVYE-ELGALFEDHNDVLIGKID-ADTHSDVADKYHITGFPTL 99 Query: 232 KIF-RKGELSSDYNGPRESNGIVKYMRAQVGPSSRDLL 342 F G Y+ R+ + + +++ + G R ++ Sbjct: 100 IWFPPDGSEPVQYSNARDVDSLTQFVSEKTGIKKRKIV 137 >SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 1|||Manual Length = 632 Score = 44.8 bits (101), Expect = 1e-05 Identities = 28/94 (29%), Positives = 46/94 (48%) Frame = +1 Query: 22 FSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCE 201 F + + A VMF + HC+ AV + I + D + +A+V+C E K C Sbjct: 189 FGNEIMSKTRAFVMFVS--LKHCEDCFHWEAVWSSITRNTDERLKMAQVNCDE-EKEMCN 245 Query: 202 KFSVSGYPTLKIFRKGELSSDYNGPRESNGIVKY 303 F + +PT ++F +G S YNGP + ++ Y Sbjct: 246 HFHIKKFPTFRVF-QGFDSIQYNGPLKYQQLLSY 278 >SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces pombe|chr 2|||Manual Length = 726 Score = 41.1 bits (92), Expect = 1e-04 Identities = 27/112 (24%), Positives = 53/112 (47%), Gaps = 4/112 (3%) Frame = +1 Query: 7 LTDSDFSSVLDQHDTALVMFYAPWCGHCKRLKP--EYAVAAGILKKDDPPVSLAKVDCTE 180 LTD+D S + + T + +Y P CG CKRL P + V + + +VDC++ Sbjct: 31 LTDNDLESEVSK-GTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVDCSK 89 Query: 181 GGKSTCEKFSVSGYPTLKIFRKGELSSD--YNGPRESNGIVKYMRAQVGPSS 330 S+C ++ PTL +++ GE+ + + ++ ++ + P + Sbjct: 90 -ELSSCA--NIRAVPTLYLYQNGEIVEEVPFGASTSEASLLDFVETHLNPDT 138 Score = 39.1 bits (87), Expect = 5e-04 Identities = 28/134 (20%), Positives = 57/134 (42%) Frame = +1 Query: 13 DSDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKS 192 D+D + L + + FY+ C C + + A ++ +++A ++C K Sbjct: 288 DADIDAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGK---LNVAHINCAVS-KR 343 Query: 193 TCEKFSVSGYPTLKIFRKGELSSDYNGPRESNGIVKYMRAQVGPSSRDLLTLADFEAFTA 372 C+++S+ +PT +F K E +Y G +V + R+ + L D Sbjct: 344 ACKQYSIQYFPTF-LFFKEEAFVEYVGLPNEGDLVSFAEEAANFEIRE-VELLDTVNAEK 401 Query: 373 KDDVVVIGFFEKES 414 DV + F++ +S Sbjct: 402 NGDVFFLYFYDDDS 415 >SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccharomyces pombe|chr 2|||Manual Length = 121 Score = 41.1 bits (92), Expect = 1e-04 Identities = 24/78 (30%), Positives = 37/78 (47%) Frame = +1 Query: 19 DFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTC 198 D+++ + +V FYA WCG CK LKP L + + S V+ + Sbjct: 27 DYNTRISADKVTVVDFYADWCGPCKYLKPFLEK----LSEQNQKASFIAVN-ADKFSDIA 81 Query: 199 EKFSVSGYPTLKIFRKGE 252 +K V PT+ +FRKG+ Sbjct: 82 QKNGVYALPTMVLFRKGQ 99 >SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces pombe|chr 1|||Manual Length = 103 Score = 38.7 bits (86), Expect = 6e-04 Identities = 21/79 (26%), Positives = 37/79 (46%) Frame = +1 Query: 16 SDFSSVLDQHDTALVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKST 195 S+F S++ Q +V F+A WCG CK + P++ + + KVD + + Sbjct: 9 SEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYS----DATFIKVDVDQLSEIA 64 Query: 196 CEKFSVSGYPTLKIFRKGE 252 E V P+ +++ GE Sbjct: 65 AEA-GVHAMPSFFLYKNGE 82 >SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosaccharomyces pombe|chr 2|||Manual Length = 290 Score = 35.5 bits (78), Expect = 0.006 Identities = 20/62 (32%), Positives = 26/62 (41%) Frame = +1 Query: 67 YAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTLKIFRK 246 YA WCG CK + P ++ A K P AKV+ E + V PT F Sbjct: 27 YADWCGPCKAISPLFSQLAS--KYASPKFVFAKVNVDE-QRQIASGLGVKAMPTFVFFEN 83 Query: 247 GE 252 G+ Sbjct: 84 GK 85 >SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr 2|||Manual Length = 244 Score = 28.3 bits (60), Expect = 0.89 Identities = 18/73 (24%), Positives = 30/73 (41%) Frame = +1 Query: 55 LVMFYAPWCGHCKRLKPEYAVAAGILKKDDPPVSLAKVDCTEGGKSTCEKFSVSGYPTLK 234 L+ FYAPW CK++ + A KD K++ E E F V+ P Sbjct: 24 LLNFYAPWAAPCKQMNQVFDQFA----KDTKNAVFLKIE-AEKFSDIAESFDVNAVPLFV 78 Query: 235 IFRKGELSSDYNG 273 + ++ + +G Sbjct: 79 LIHGAKVLARISG 91 >SPAPB18E9.02c |ppk18||serine/threonine protein kinase Ppk18 |Schizosaccharomyces pombe|chr 1|||Manual Length = 1316 Score = 27.9 bits (59), Expect = 1.2 Identities = 13/42 (30%), Positives = 22/42 (52%) Frame = -3 Query: 477 VSKCNLFSHFISSLEEVSFKIRLLFKETNDNNIILSGECFKV 352 VSK NLFS I L+ F++ ++ E +++ E F + Sbjct: 1203 VSKLNLFSELIKLLKSYKFQVSIVTDEDKMLRTLMADEKFSI 1244 >SPCC553.04 |cyp9||WD repeat containing cyclophilin family peptidyl-prolyl cis-trans isomerase Cyp9|Schizosaccharomyces pombe|chr 3|||Manual Length = 610 Score = 27.5 bits (58), Expect = 1.6 Identities = 12/37 (32%), Positives = 20/37 (54%) Frame = -1 Query: 503 FSRTSLAEECPNVTSSLILSAVLRKSPLRSDSFSKKP 393 F+R SL ++ P ++ L + +PL +SF K P Sbjct: 366 FTRLSLYQQAPKKSNLPSLDVIASNNPLVEESFQKDP 402 >SPAC13C5.03 |tht1||nuclear membrane protein involved in karyogamy |Schizosaccharomyces pombe|chr 1|||Manual Length = 543 Score = 27.1 bits (57), Expect = 2.1 Identities = 14/64 (21%), Positives = 33/64 (51%) Frame = -3 Query: 510 SNLLKDLLSRGVSKCNLFSHFISSLEEVSFKIRLLFKETNDNNIILSGECFKVSQSK*IS 331 S +++ + +SKC+L + ++ + I++ + + I+ EC + SQS+ +S Sbjct: 29 STCYQEVAATLISKCSLLNTELTIDNRIHSAIQMTLCDFERSQILAPSECVRGSQSECVS 88 Query: 330 RAGS 319 + S Sbjct: 89 KLES 92 >SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces pombe|chr 1|||Manual Length = 279 Score = 26.2 bits (55), Expect = 3.6 Identities = 13/34 (38%), Positives = 17/34 (50%) Frame = +1 Query: 187 KSTCEKFSVSGYPTLKIFRKGELSSDYNGPRESN 288 KS EK + Y LK+ + E + DYN P N Sbjct: 37 KSPLEKEIANEYEALKVTERKEDTQDYNEPELHN 70 >SPBC1683.01 |||inorganic phosphate transporter |Schizosaccharomyces pombe|chr 2|||Manual Length = 573 Score = 25.8 bits (54), Expect = 4.8 Identities = 13/38 (34%), Positives = 19/38 (50%) Frame = +1 Query: 463 VTFGHSSAKEVLEKVGYKNNVVLYRPKRLQNKFEESSA 576 VT GH E E ++N V P+ ++ +FE SA Sbjct: 292 VTKGHPEIHESSENYLSRSNTVENEPENIEKQFESVSA 329 >SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||Manual Length = 534 Score = 25.4 bits (53), Expect = 6.3 Identities = 18/56 (32%), Positives = 26/56 (46%) Frame = -1 Query: 500 SRTSLAEECPNVTSSLILSAVLRKSPLRSDSFSKKPMTTTSSLAVNASKSAKVSRS 333 S ++ + P TSS LS+ S S S S T+SSL+ ++ S S S Sbjct: 193 SSSTFSSAAPTSTSSSYLSSSSVVSSSSSPSSSSSSTLTSSSLSTSSIPSTSSSSS 248 >SPBC215.08c |arg4||carbamoyl-phosphate synthase Arg4|Schizosaccharomyces pombe|chr 2|||Manual Length = 1160 Score = 25.4 bits (53), Expect = 6.3 Identities = 10/34 (29%), Positives = 17/34 (50%) Frame = +1 Query: 481 SAKEVLEKVGYKNNVVLYRPKRLQNKFEESSAVY 582 S L K+G+ +V Y P+ + F+E +Y Sbjct: 653 SCARTLRKLGHSTIMVNYNPETVSTDFDECERLY 686 >SPAPB15E9.01c ||SPAPB18E9.06c|sequence orphan|Schizosaccharomyces pombe|chr 1|||Manual Length = 1036 Score = 25.0 bits (52), Expect = 8.3 Identities = 17/47 (36%), Positives = 23/47 (48%) Frame = -1 Query: 464 TSSLILSAVLRKSPLRSDSFSKKPMTTTSSLAVNASKSAKVSRSLEL 324 TSS + S S S S +TTS+ A ++S S+ VS S L Sbjct: 209 TSSSLSSTAASNSATSSSLASSSLNSTTSATATSSSISSTVSSSTPL 255 Database: spombe Posted date: Oct 4, 2007 10:57 AM Number of letters in database: 2,362,478 Number of sequences in database: 5004 Lambda K H 0.316 0.134 0.390 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 2,376,062 Number of Sequences: 5004 Number of extensions: 47117 Number of successful extensions: 151 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 136 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 146 length of database: 2,362,478 effective HSP length: 69 effective length of database: 2,017,202 effective search space used: 258201856 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.6 bits)
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