BLASTX 2.2.12 [Aug-07-2005] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= I10A02NGRL0001_I19 (624 letters) Database: arabidopsis 28,952 sequences; 12,070,560 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value At5g51250.1 68418.m06354 kelch repeat-containing F-box family pr... 29 3.3 At3g55220.1 68416.m06133 splicing factor, putative contains CPSF... 28 5.8 At3g55200.1 68416.m06131 splicing factor, putative contains CPSF... 28 5.8 At1g10300.1 68414.m01160 GTP-binding protein-related contains si... 28 5.8 >At5g51250.1 68418.m06354 kelch repeat-containing F-box family protein similar to SKP1 interacting partner 6 [Arabidopsis thaliana] GI:10716957; contains Pfam profiles PF01344: Kelch motif, PF00646: F-box domain Length = 368 Score = 28.7 bits (61), Expect = 3.3 Identities = 15/41 (36%), Positives = 22/41 (53%), Gaps = 4/41 (9%) Frame = -3 Query: 592 NTHHIAKCVRKE*NISNNVLKKKQS----VAGSKQPHDPPA 482 NTH C + + ++N+ KKK+S +A PH PPA Sbjct: 65 NTHWFTLCRKPDGTLTNDTSKKKKSNGYVLATVPIPHSPPA 105 >At3g55220.1 68416.m06133 splicing factor, putative contains CPSF A subunit region (PF03178); contains weak WD-40 repeat (PF00400); similar to Splicing factor 3B subunit 3 (SF3b130)/spliceosomal protein/Splicing factor 3B subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens, EMBL:HSAJ1443_1 Length = 1214 Score = 27.9 bits (59), Expect = 5.8 Identities = 15/35 (42%), Positives = 19/35 (54%), Gaps = 2/35 (5%) Frame = +1 Query: 400 YYEGDWGGELISSSGTSARGAVACL--FRVPEGRE 498 Y+E D G+L+ G VACL VPEGR+ Sbjct: 563 YFEADMTGQLMEVEKHEMSGDVACLDIAPVPEGRK 597 >At3g55200.1 68416.m06131 splicing factor, putative contains CPSF A subunit region (PF03178); contains weak WD-40 repeat (PF00400); similar to Splicing factor 3B subunit 3 (SF3b130)/spliceosomal protein/Splicing factor 3B subunit 3 (SAP 130)(KIAA0017)(SP:Q15393) Homo sapiens, EMBL:HSAJ1443_1 Length = 1214 Score = 27.9 bits (59), Expect = 5.8 Identities = 15/35 (42%), Positives = 19/35 (54%), Gaps = 2/35 (5%) Frame = +1 Query: 400 YYEGDWGGELISSSGTSARGAVACL--FRVPEGRE 498 Y+E D G+L+ G VACL VPEGR+ Sbjct: 563 YFEADMTGQLMEVEKHEMSGDVACLDIAPVPEGRK 597 >At1g10300.1 68414.m01160 GTP-binding protein-related contains similarity to nucleolar GTP-binding protein 1 SP: Q9BZE4 from [Homo sapiens]; Length = 687 Score = 27.9 bits (59), Expect = 5.8 Identities = 12/40 (30%), Positives = 20/40 (50%) Frame = +2 Query: 350 MFRIVCLPLCLCFAVCRITRGIGEGS*LVRQERRRAERLP 469 ++R CL + +C + +GIG + Q R+ RLP Sbjct: 152 LYRCKCLKVAALGRMCTVMKGIGPSLAYLEQVRQHIARLP 191 Database: arabidopsis Posted date: Oct 4, 2007 10:56 AM Number of letters in database: 12,070,560 Number of sequences in database: 28,952 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.279 0.0580 0.190 Matrix: BLOSUM62 Gap Penalties: Existence: 9, Extension: 2 Number of Hits to DB: 10,050,213 Number of Sequences: 28952 Number of extensions: 167506 Number of successful extensions: 416 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 408 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 416 length of database: 12,070,560 effective HSP length: 78 effective length of database: 9,812,304 effective search space used: 1265787216 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 37 (14.9 bits) X3: 62 (25.0 bits) S1: 41 (21.7 bits)
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